The polycomb protein complex interacts with GATA-6/PPARα to inhibit α-MHC expression.

Transcription factors collaborate with epigenetic regulatory factors to orchestrate cardiac differentiation for heart development, but the underlying mechanism is not fully understood. Here, we report that GATA-6 induces cardiac differentiation but peroxisome proliferator-activated receptor α (PPARα) reverses GATA-6-induced cardiac differentiation, possibly because GATA-6/PPARα recruits the polycomb protein complex containing EZH2/Ring1b/BMI1 to the promoter of the cardiac-specific α-myosin heavy chain (α-MHC) gene and suppresses α-MHC expression, which ultimately inhibits cardiac differentiation. Furthermore, Ring1b ubiquitylates PPARα and GATA-6.

Reference Materials for Improving Reliability of Multiomics Profiling.

High-throughput technologies for multiomics or molecular phenomics profiling have been extensively adopted in biomedical research and clinical applications, offering a more comprehensive understanding of biological processes and diseases. Omics reference materials play a pivotal role in ensuring the accuracy, reliability, and comparability of laboratory measurements and analyses. However, the current application of omics reference materials has revealed several issues, including inappropriate selection and underutilization, leading to inconsistencies across laboratories.

Improved prediction of swimming talent through random forest analysis of anthropometric and physiological phenotypes.

Unlabelled: The field of competitive swimming lacks broadly applicable predictive models for talent identification across various age groups of adolescent swimmers. This study aimed to construct a predictive model for athletic talent using machine learning methods based on anthropometric and physiological data. Baseline data were collected from 5444 participants aged 10-18 in Shanghai, China, between 2015 and 2018, with 4969 completing a 3-year follow-up.

Prognostic Significance of Polymorphism rs140693 and Clinical Characteristics in Chinese Lung Cancer Patients.

Unlabelled: Lung cancer remains the leading cause of death among cancer patients, and the five-year survival rate is less than 25%. However, () polymorphism rs140693 predicts the prognosis of lung cancer patients still needs further verification. Primary lung cancer patients ( = 839) were collected from two hospitals, genomic DNA was extracted from blood, and genotyping was performed using SNPcan technology.

Ring finger protein 5 mediates STING degradation through ubiquitinating K135 and K155 in a teleost fish.

Stimulator of interferon genes (STING) is a key connector protein in interferon (IFN) signaling, crucial for IFN induction during the activation of antiviral innate immunity. In mammals, ring finger protein 5 (RNF5) functions as an E3 ubiquitin ligase, mediating STING regulation through K150 ubiquitylation to prevent excessive IFN production. However, the mechanisms underlying RNF5's regulation of STING in teleost fish remain unknown.

Oral Intake of Collagen and Collagen Hydrolysate From Attenuates Ultraviolet-Induced Skin Photoaging in Mice.

With the rapid emergence of pufferfish aquaculture and processing industries, fish skin is underutilized as a byproduct of processing, leading to resource waste. In this study, skin collagen (TBSC) was extracted by acetic acid solubilization and its physicochemical properties were analyzed. The effects of TBSC and the TBSC hydrolysate (TBSCH) on ultraviolet (UV) irradiation-induced photoaging were investigated using a mouse model.

The movement and distribution of pregnant spotted ragged-tooth sharks, , in the iSimangaliso Wetland Park, South Africa.

The spotted ragged-tooth shark, , is widely distributed in subtropical continental coastal seas. In South Africa, it is commonly found along the entire south and east coasts, including the iSimangaliso Wetland Park (IWP) in the far north, which is the largest Marine Protected Area on the South African coast. Pregnant females occur there for much of the year, with the largest aggregations in summer.

Deciphering metabolic pathways: A treasure map to therapeutic targets.

Indian tick typhus is an infectious disease caused by intracellular gram-negative bacteria (). The bacterium is transmitted to humans through bite of infected ticks and sometimes by lice, fleas or mites. The disease is restricted to some areas with few cases but in last decade it is re-emerging with large number of cases from different areas of India.

Detailed cytogenetic analysis of three duck species (the northern pintail, mallard, and common goldeneye) and karyotype evolution in the family Anatidae (Anseriformes, Aves).

Galliformes and Anseriformes are two branches of the Galloanserae group, basal to other Neognathae. In contrast to Galliformes, Anseriformes have not been thoroughly researched by cytogenetic methods. This report is focused on representatives of Anseriformes and the evolution of their chromosome sets.

[Evaluation of the toxicity of perfluorooctanoic acid toward human colorectal cancer cells using multi-dimensional approaches].

While human exposure to perfluorooctanoic acid (PFOA) can lead to ulcerative colitis, the molecular mechanisms responsible for PFOA-induced intestinal toxicity are unclear. Herein, we examined the toxicity of PFOA toward human colorectal cancer cells (HCT116) from three dimensions: the cytotoxic phenotype, cell respiration, and transcription levels of metabolism-related genes. Formazan was used to assess how PFOA exposure affects HCT116-cell relative viability, after which the mitochondrial respiratory activities of these cells were determined by analyzing extracellular flux.

[Solid-phase extraction coupled with high performance liquid chromatography-triple quadrupole mass spectrometry for simultaneous determination of seven coumarins in water samples from drinking water treatment plants].

Chlorinated coumarins, which are as cytotoxic as highly toxic halobenzoquinones toward CHO-K1 cells, have recently been identified as disinfection byproducts in drinking water disinfection processes. Therefore, detecting coumarins in water samples collected at various stages from drinking water treatment plants helps assess the formation of chlorinated coumarins in drinking water. Hence, a simple, rapid, accurate, and sensitive method for quantifying coumarins in water samples is required.

Marine microalgae - mediated biodegradation of polystyrene microplastics: Insights from enzymatic and molecular docking studies.

Biodegradation of microplastics (MPs) through microalgal strains would be of eco-friendly approach for significant pollution abatement. Polystyrene (PS) is a major contaminant in the marine environment; however studies on marine microalgal degradation of PS MPs have been very limited. In the present study, six marine microalgal strains viz.

Characterization of Arabidopsis thaliana FRATAXIN HOMOLOG in heme catabolism.

Frataxin plays vital roles in various iron related processes. Arabidopsis thaliana FRATAXIN HOMOLOG (AtFH) is the first identified plant frataxin and has been found to regulate the last step of heme biosynthesis. Here, we report of the involvement of AtFH in heme catabolism by regulating the activity of heme oxygenase.

In vivo self-assembled siRNAs within small extracellular vesicles attenuate LRRK2-induced neurodegeneration in Parkinson's disease models.

Rationale: Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene play an important role in Parkinson's disease (PD) pathogenesis, and downregulation of LRRK2 has become a promising therapy for PD. Here, we developed a synthetic biology strategy for the self-assembly and delivery of small interfering RNAs (siRNAs) of LRRK2 into the substantia nigra via small extracellular vesicles (sEVs) using a genetic circuit (in the form of naked DNA plasmid) to attenuate PD-like phenotypes in mouse model.

Methods: We generated the genetic circuit encoding both a neuron-targeting rabies virus glycoprotein (RVG) tag and a LRRK2 siRNA under the control of a cytomegalovirus (CMV) promoter, and assessed its therapeutic effects using LRRK2 mouse models of PD.

Macroevolutionary inference of complex modes of chromosomal speciation in a cosmopolitan plant lineage.

The effects of single chromosome number change-dysploidy - mediating diversification remain poorly understood. Dysploidy modifies recombination rates, linkage, or reproductive isolation, especially for one-fifth of all eukaryote lineages with holocentric chromosomes. Dysploidy effects on diversification have not been estimated because modeling chromosome numbers linked to diversification with heterogeneity along phylogenies is quantitatively challenging.

A comprehensive review on microbial hyaluronan-degrading enzymes: from virulence factors to biotechnological tools.

Hyaluronan (HA), a natural high molecular weight polysaccharide, has extensive applications in cosmetology and medical treatment. Hyaluronan-degrading enzymes (Hyals) act as molecular scissors that cleave HA by breaking the glucosidic linkage. Hyals are present in diverse organisms, including vertebrates, invertebrates and microorganisms, and play momentous roles in biological processes.

The GrassSyn dataset: Soil organic carbon stocks in Brazilian grassy ecosystems.

Although ecosystem management and restoration are known to enhance carbon storage, limited knowledge of ecosystem-specific soil organic carbon (SOC) stocks and processes hinders the development of climate-ready, biodiversity-focused policies. Baseline SOC stocks data for specific ecosystems is essential. This paper aims to: (i) examine SOC stock variability across major grassy ecosystems in Brazil and (ii) discuss data limitations and applications.

Microbiota-dependent and -independent autoimmunity in plants.

In a recent study to identify arabidopsis (Arabidopsis thaliana) genes involved in maintaining normal leaf microbiota, Cheng et al. discovered TIP GROWTH DEFECTIVE1 (TIP1) encoding an S-acyltransferase. The tip1 mutant exhibits abnormal microbiota levels and phenotypes resembling autoimmune mutants.

New insight of electrogenerated HO into oxychlorides inhibition and decontamination promotion: From radical to nonradical pathway during anodic oxidation of high Cl-laden wastewater process.

Anodic oxidation (AO) has been extensively hailed as a robust and promising technology for pollutant degradation, but the parasitic formation of oxychlorides (ClO) would induce a seriously over-evaluated electrochemical COD removal performance and dramatical biotoxicity increasement of the AO-treated Cl-laden effluents. Herein, we shed new light on the roles of HO high-efficiently electrogenerated in three-dimensional (3D) reactor in inhibiting ClO production and promoting pollutant degradation, which has been overlooked in previous literature. Total yield of ClO in phenol simulated wastewater containing 30 mM Cl was dropped from 25 mM and 24.

SDF-1/CXCR4 axis maintains porcine prospermatogonia undifferentiated state through regulation of transcription suppressor PLZF.

Prospermatogonia (ProSGs), the progenitors of spermatogonial stem cells in neonatal testes, undergo critical migration to the testicular microenvironment-a fundamental process for testicular development and subsequent spermatogenic capacity. The SDF-1/CXCR4 chemokine axis serves as an essential molecular guidance mechanism, directing ProSGs toward the basal membrane of seminiferous tubules. Nevertheless, the precise molecular mechanisms governing this axis remain incompletely understood.

Developing dual-responsive quinolinium prodrugs of 8-hydroxyquinoline by harnessing the dual chelating sites.

The bidentate metal ion chelator 8-hydroxyquinoline (8-HQ) demonstrates significant potential in anticancer therapy but is hindered by adverse effects due to nonspecific chelation in normal tissues. The phenolic hydroxyl oxygen of 8-HQ has been extensively exploited to develop O-masked 8-HQ prodrugs aimed at achieving on-demand chelation. However, the equally crucial quinoline nitrogen for chelation remains underutilized.

Ultrasound-assisted extraction of polysaccharide from Allium chinense G. Don epidermal waste: Evaluation of extraction mechanism, physicochemical properties, and bioactivities.

Allium chinense G. Don waste (ACGD) has an abundance of polysaccharides (ACGDP). Therefore, in this study, a method for extraction of ACGDP from ACGD by ultrasonic-assisted hot water extraction (UAE) based on ultrasonic cleaning equipment was developed.

Tryptanthrins as multi-bioactive agents: discovery, diversity distribution and synthesis.

Tryptanthrin and its derivatives, representing a type of alkaloids with indoloquinazoline structures, were first obtained from blue plants and indigo, and then extracted from fungi, marine bacteria and a number of many other natural sources. Various strategies for their chemical synthesis have been reported while tryptanthrin biosynthesis has been less investigated. Tryptanthrin and its derivative products have a broad range of pharmacological and biological functions.

A sequence-activatable dual-locked fluorescent probe for simultaneous detection of hypochlorous acid and peroxynitrite during drug-induced liver injury.

Drug-induced liver injury (DILI) is a crucial factor that poses a significant threat to human health. DILI process leads to the changes of reactive oxygen species and reactive nitrogen species content in cells, which leads to oxidative and nitrosative stress in cells. However, the high reactivity of hypochlorous acid (HOCl) and peroxynitrite (ONOO⁻), combined with a lack of in situ imaging techniques, has hindered a detailed understanding of their roles in DILI.

Functional Monomers Equipped Microgel System for Managing Parkinson's Disease by Intervening Chemokine Axis-mediated Nerve Cell Communications.

The complex pathology of Parkinson's disease (PD) requires comprehensive understanding and multi-pronged interventions for communication between nerve cells. Despite new developments in nanotechnology in the treatment of PD, in-depth exploration of their biological effects, in particular, the specific mechanisms of inflammation inhibition are lacking. Herein, using the stable cascade catalysis channel formed by polydopamine (PDA), imidazole groups, and Cu ions, a microgel system comprising functional monomers [superoxide dismutase (SOD) with double bonds, PDA, 2-methacryloyloxy ethyl phosphorylcholine (MPC), and Cu ions] is proposed for managing PD.