Efficient and easy-to-use capturing three-dimensional metagenome interactions with GutHi-C.

Hi-C can obtain three-dimensional chromatin structure information and is widely used for genome assembly. We constructed the GutHi-C technology. As shown in the graphical abstract, it is a highly efficient and quick-to-operate method and can be widely used for human, livestock, and poultry gut microorganisms.

TurboID-mediated proximity labeling for screening interacting proteins of FIP37 in .

Proximity labeling was recently developed to detect protein-protein interactions and members of subcellular multiprotein structures in living cells. Proximity labeling is conducted by fusing an engineered enzyme with catalytic activity, such as biotin ligase, to a protein of interest (bait protein) to biotinylate adjacent proteins. The biotinylated protein can be purified by streptavidin beads, and identified by mass spectrometry (MS).

FLS2-RBOHD module regulates changes in the metabolome of in response to abiotic stress.

Through crosstalk, FLAGELLIN SENSITIVE 2 (FLS2) and RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) are involved in regulating the homeostasis of cellular reactive oxygen species (ROS) and are linked to the metabolic response of plants toward both biotic and abiotic stress. In the present study, we examined the metabolome of seedlings under drought and salt conditions to better understand the potential role of FLS2 and RBOHD-dependent signaling in the regulation of abiotic stress response. We identified common metabolites and genes that are regulated by FLS2 and RBOHD, and are involved in the response to drought and salt stress.

Prescribed fire alters nematode communities in an old-field grassland.

Fire is a common disturbance in many biomes, with both beneficial and detrimental effects on soil biology, which largely depend on fire intensity. However, little is known about the impact of fire on soil nematode communities in terrestrial ecosystem. In the present study, we investigated the effects of short-term prescribed fire on soil nematode communities and soil properties in an old-field grassland in Northern China.

: A method for quantifying putative biotic associations of microbes at the community level.

A workflow has been compiled as "qcmi" R package-the uantifying ommunity-level icrobial nteractions-to identify and quantify the putative biotic associations of microbes at the community level from ecological networks.

Protective effect of Oliver male flowers on ethanol-induced DNA damage in mouse cerebellum and cerebral cortex.

Ethanol is a principal ingredient of alcoholic beverages with potential neurotoxicity and genotoxicity, and the ethanol-associated oxidative DNA damage in the central nervous system is well documented. Natural product may offer new options to protect the brain against ethanol-induced neurotoxicity. The male flower of (EUF) Oliver has been extensively utilized as the tea, the healthy hot drink on the market.

Dynamics, gene transfer, and ecological function of intracellular and extracellular DNA in environmental microbiome.

Extracellular DNA (eDNA) and intracellular DNA (iDNA) extensively exist in both terrestrial and aquatic environment systems and have been found to play a significant role in the nutrient cycling and genetic information transmission between the environment and microorganisms. As inert DNA sequences, eDNA is able to present stability in the environment from the ribosome enzyme lysis, therein acting as the historical genetic information archive of the microbiome. As a consequence, both eDNA and iDNA can shed light on the functional gene variety and the corresponding microbial activity.

Brain co-delivery of first-line chemotherapy drug and epigenetic bromodomain inhibitor for multidimensional enhanced synergistic glioblastoma therapy.

Glioblastoma (GBM) is a central nervous system tumor with poor prognosis due to the rapid development of resistance to mono chemotherapy and poor brain targeted delivery. Chemoimmunotherapy (CIT) combines chemotherapy drugs with activators of innate immunity that hold great promise for GBM synergistic therapy. Herein, we chose temozolomide, TMZ, and the epigenetic bromodomain inhibitor, OTX015, and further co-encapsulated them within our well-established erythrocyte membrane camouflaged nanoparticle to yield ApoE peptide decorated biomimetic nanomedicine (ABNM@TMZ/OTX).

Correction: SERS-based nanostrategy for rapid anemia diagnosis.

Correction for 'SERS-based nanostrategy for rapid anemia diagnosis' by Pir Muhammad , , 2020, , 1948-1957, DOI: 10.1039/C9NR09152A.

Receptor-targeting nanomaterials alleviate binge drinking-induced neurodegeneration as artificial neurotrophins.

The distinguished properties of nanomaterials promote us to explore whether their intrinsic activities would be beneficial to disease treatment. Furthermore, understanding the molecular mechanism is thereby crucial for biomedical applications. Here, we investigate the therapeutic effects of single-walled carbon nanotubes (SWNTs) in a rat model of binge alcohol-induced neurodegeneration.

From mouse to mouse-ear cress: Nanomaterials as vehicles in plant biotechnology.

Biological applications of nanomaterials as delivery carriers have been embedded in traditional biomedical research for decades. Despite lagging behind, recent significant breakthroughs in the use of nanocarriers as tools for plant biotechnology have created great interest. In this Perspective, we review the outstanding recent works in nanocarrier-mediated plant transformation and its agricultural applications.

Direct effects of nitrogen addition on seed germination of eight semi-arid grassland species.

Seed germination plays an important role in mediating plant species composition of grassland communities under nitrogen (N) enrichment. Shifts of plant community structure with N-enhanced deposition in terrestrial ecosystems have occurred globally. Despite numerous studies about the effects of enhanced N deposition on mature plant communities, few studies have focused on seed germination.

Mass loss and nutrient release during the decomposition of sixteen types of plant litter with contrasting quality under three precipitation regimes.

Mass loss and nutrient release during litter decomposition drive biogeochemical cycling in terrestrial ecosystems. However, the relationship between the litter decomposition process and the decomposition stage, precipitation, and litter quality has rarely been addressed, precluding our understanding of how litter decomposition regulates nutrient cycling in various ecosystems and their responses to climate change. In this study, we measured mass loss as well as carbon and nutrient releases during the decomposition of 16 types of leaf litter under three precipitation treatments over 12 months in a common garden experiment (i.

SERS-based nanostrategy for rapid anemia diagnosis.

Iron detection is one of the critical markers to diagnose multiple blood-related disorders that correspond to various biological dysfunctions. The currently available anemia detection approach can be used only for pre-treated blood samples that interfere with the actual iron level in blood. Real-time detection approaches with higher sensitivity and specificity are certainly needed to cope with the commercial level clinical analyses.

Litter addition decreases plant diversity by suppressing seeding in a semiarid grassland, Northern China.

Unlabelled: Plant community diversity is conducive to maintain the regional ecosystems stability and ecosystem services. Seed germination is one of the main ways to regulate plant diversity, owing to seedling recruitment as a basis for plant community renewal. However, the exact mechanism of how plant litter affects seedling recruitment, and species richness is not yet fully understood.

Nitrogen addition decreases seed germination in a temperate steppe.

Seed germination and seedling establishment play an important role in driving the responses of plant community structure and function to global change. Nitrogen (N) deposition is one of the driving factors of global change, which often leads to a loss in species richness in grassland ecosystems. However, how seed germination responds to N addition remains unclear.

Fire rather than nitrogen addition affects understory plant communities in the short term in a coniferous-broadleaf mixed forest.

Increasing fire risk and atmospheric nitrogen (N) deposition have the potential to alter plant community structure and composition, with consequent impacts on biodiversity and ecosystem functioning. This study was conducted to examine short-term responses of understory plant community to burning and N addition in a coniferous-broadleaved mixed forest of the subtropical-temperate transition zone in Central China. The experiment used a pair-nested design, with four treatments (control, burning, N addition, and burning plus N addition) and five replicates.

Effects of nitrogen addition and mowing on rodent damage in an Inner Mongolian steppe.

Rodent damage is a serious threat to sustainable management of grassland. The effects of nitrogen (N) deposition and grassland management on rodent damage have been scarcely studied. Here, we reported the effects of 2 years of N addition and mowing on burrow density and damage area of in a semiarid steppe in Inner Mongolia.

Long-term antagonistic effect of increased precipitation and nitrogen addition on soil respiration in a semiarid steppe.

Changes in water and nitrogen (N) availability due to climate change and atmospheric N deposition could have significant effects on soil respiration, a major pathway of carbon (C) loss from terrestrial ecosystems. A manipulative experiment simulating increased precipitation and atmospheric N deposition has been conducted for 9 years (2005-2013) in a semiarid grassland in Mongolian Plateau, China. Increased precipitation and N addition interactively affect soil respiration through the 9 years.