[Different effect of galanin on the proliferation of PC12 and B104 cells].

The effect of galanin (GAL) on neural proliferation was studied in this article using PC12 and B104 cells. RT-PCR was used to determine the expression of galanin and its receptors in both cells. MTT analysis method was employed to detect the effects of galanin and the agonist, antagonist of galanin receptors on the proliferation of both cells.

[Galanin increases the survival rate of hippocampal cells injured by H2O2 in vitro].

The method of primary hippocampal nerve cell culture was used to study the injury effect of H2O2 and the protective effect of galanin (GAL) and GAL receptor agonists. Result demonstrated that H2O2 has obvious dose relative toxicity to hippocampal cells in vitro. GAL and GAL's nonselective agonist GAL1-11, GalR2's selective agonist GAL2-11 can increase the survival rate of hippocampal cells suffered form H2O2.

[The expression of galanin and galanin receptors in olfactory ensheathing cells and effects of galanin on cells proliferation].

Olfactory ensheathing cells (OECs) were isolated from newborn rat olfactory bulb and cultured in vitro. RT-PCR was used to determine the expression of galanin and it's receptors in these cells. MTT analysis was used to detect the effects of galanin and agonist, antagonist of galanin receptors on the proliferation of OECs.