Isolation and characterization of cells derived from human epithelial rests of Malassez.

The epithelial rests of Malassez (ERMs) might represent a valuable source of oral epithelial cells with stem cell properties. The purpose of this study was to isolate and characterize cells derived from human ERM, and compare them with cells derived from matched normal oral mucosa (NOM). Matched tissue specimens of the periodontal ligament of extracted tooth and NOM were collected.

Influence of the difference between implant body and screw materials on abutment screw loosening.

The purpose of this study was to investigate how differences in dental implant and screw materials affected screw loosening. Screws (pure titanium; Ti4S, titanium alloy; TiAS), blocks (Y-TZP; ZrB, pure titanium; Ti4B) and plates (Y-TZP; ZrP), representing abutment screws, implant bodies and superstructures, respectably, were used. Plates were fastened to blocks by screws using a torque of 20 N•cm, and the loosening torque was measured after cyclic loading.

Histopathological findings of an exposed human pulp carbonised by CO laser irradiation: A case report.

This report shows the healing process of an exposed pulp carbonised by CO laser irradiation prior to the application of a capping material. Six intact teeth from four volunteers were irradiated by CO laser and randomly capped with either an adhesive resin (SE bond) (n = 3) or calcium hydroxide-based cement (Dycal) (n = 3). The laser was operated in super-pulsed mode (power output, 0.

The effect of differences in the number of fiber bundles of the anterior tibial ligament on ankle braking function: a simulation study.

Purpose: The aim was to clarify the effect of differences in the number of fiber bundles of the anterior tibial ligament (ATFL) on ankle braking function.

Methods: The study sample included 81Japanese cadavers. ATFLs were categorized as: Type I with one fiber bundle; Type II with two fiber bundles that were completely separated; and Type III with three fiber bundles.

Dental pulp cell bank as a possible future source of individual hepatocytes.

Mesenchymal stem cells (MSCs) as a source for regenerative medicine are now the subject of much clinical attention. There are high expectations due to their safety, low tumorigenic risk, and low ethical concerns. MSC therapy has been approved for acute graft-versus host diseases since 2015.

Polytetrafluoroethylene (PTFE): A resin material for possible use in dental prostheses and devices.

Polytetrafluoroethylene (PTFE) is chemically stable, non-toxic to humans, highly resistant to heat and chemicals, and has an extremely low coefficient of friction. Therefore, PTFE is used in medical applications. We focused on the physical properties of PTFE in relation to its application as a material for use in prostheses and dental devices/instruments.

Morphological characteristics of the lateral talocalcaneal ligament: a large-scale anatomical study.

Purpose: The purpose of this study is to clarify the morphological characteristics of the lateral talocalcaneal ligament (LTCL).

Methods: This study examined 100 legs from 54 Japanese cadavers. The LTCL was classified into three types: Type I, the LTCL branches from the calcaneofibular ligament (CFL); Type II, the LTCL is independent of the CFL and runs parallel to the calcaneus; and Type III, the LTCL is absent.

Simultaneous monitoring of Ca responses and salivary secretion in live animals reveals a threshold intracellular Ca concentration for salivation.

New Findings: What is the central question of this study? The effects of Ca responses on salivary fluid secretion have been studied indirectly by monitoring ion channel activities and other indices. Therefore, Ca responses during salivary secretion remain poorly understood. What is the main finding and its importance? Herein, we developed a simultaneous monitoring system for Ca responses and salivary secretion in live animals using a YC-Nano50-expressing submandibular gland and a fibre-optic pressure sensor.

Thermoforming method to effectively maintain mouthguard thickness: Effect of moving the model position just before vacuum formation.

Background/aims: Mouthguards can reduce the risk of sports-related injuries but the sheet material and thickness have a large effect on their efficacy and safety. The aim of this study was to investigate the effect of the thermoforming technique that moves the model position just before vacuum formation.

Materials And Methods: Ethylene vinyl acetate sheets of 4.

Recombinant human BMP9 (RhBMP9) in comparison with rhBMP2 for ridge augmentation following tooth extraction: An experimental study in the Beagle dog.

Objectives: The purpose of this study was to compare the effects of rhBMP2 with rhBMP9 on ridge augmentation following healing of extraction sockets in dogs.

Material And Methods: Five male Beagle dogs, approximately 12 months of age, were used. The mesial roots of the four maxillary premolars were endodontically treated.

Usefulness of shear wave elastography in the diagnosis of oral and maxillofacial diseases.

Purpose: To evaluate the usefulness of shear wave elastography in the diagnosis of oral and maxillofacial diseases.

Materials And Methods: Ten patients with oral and maxillofacial diseases and 28 volunteers drawn from our student doctors were examined by shear wave elastography with a 14-MHz linear transducer using an Aplio 300 apparatus (Canon Medical Systems, Otawara, Japan). A statistical analysis of the shear elastic modulus (kPa) of healthy tissue (the sublingual gland, submandibular gland, anterior belly of the digastric muscle, and geniohyoid muscle) in the 28 volunteers was performed using 1-way repeated measures analysis of variance with the Tukey honest significant difference test.

Five-axis laser milling system that realizes more accurate zirconia CAD/CAM crowns by direct milling from fully sintered blocks.

Directly milling zirconia computer-aided design (CAD)/computer-aided manufacturing (CAM) crowns from fully sintered zirconia blocks using a five-axis laser milling system, compared with three-axis milling and full sintering by heating milled semi-sintered crowns, was investigated. The mechanical characteristics of zirconia specimens were similar across groups. The order of the marginal gap was three-axis>conventional (lingual thickness of 1.

Morphological features of the bifurcated ligament.

Introduction: Damage to the bifurcate ligament is one of the most difficult injuries to diagnose from imaging techniques. A probable reason for this is that the morphological characteristics of this structure have yet to be sufficiently elucidated. We, therefore, endeavored to elucidate the morphological characteristics of the bifurcate ligament through a large-scale study involving numerous specimens.

Effects of the percentage of air/water in spray on the efficiency of tooth ablation with erbium, chromium: yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser irradiation.

We aimed at examining the effects of a percentage of air/water in spray on the cutting efficiency of Er,Cr:YSGG laser for enamel and dentin. The intensity and frequency of irradiation were 3.0 W and 20 Hz for the enamel surface and 2.

White-to-opaque switching is involved in the phospholipase B production of Candida dubliniensis on Price's egg yolk agar.

Measuring the production of Candida dubliniensis (C. dubliniensis) phospholipase B (PLase B) by the Price's method has long been considered to be unattainable because the levels of PLase produced are undetectable. In this study, C.

Immunolocalization of enamel matrix protein-like proteins in the tooth enameloid of spotted gar, Lepisosteus oculatus, an actinopterygian bony fish.

Enameloid is a well-mineralized tissue covering the tooth surface in fish and it corresponds to the outer-most layer of dentin. It was reported that both dental epithelial cells and odontoblasts are involved in the formation of enameloid. Nevertheless, the localization and timing of secretion of ectodermal enamel matrix proteins in enameloid are unclear.

[Advances in methods for analyzing IP signaling and understanding of coupled Ca and IP oscillations].

Inositol 1,4,5-trisphosphate (IP) is an important intracellular messenger produced by phospholipase C via the activation of G-protein-coupled receptor- or receptor-tyrosine-kinase-mediated pathways, and is involved in numerous responses to hormones, neurotransmitters, and growth factors through the releases of Ca from intracellular stores via IP receptors. IP-mediated Ca signals often exhibit complex spatial and temporal organizations, such as Ca oscillations. Recently, new methods have become available to measure IP concentration ([IP]) using AlphaScreen technology, fluorescence polarization, and competitive ligand binding assay (CFLA).

Hard and soft tissue responses to implant made of three different materials with microgrooved collar in a dog model.

The objective of the present study was to assess hard and soft tissue around dental implants made of three different materials with microgrooves on the collar surface. Microgrooved implants were inserted in the mandibles of five male beagles. Implants were made of three kinds of material; titanium (Ti), yttria-stabilized tetragonal zirconia polycrystals (Y-TZP) and ceria partially stabilized zirconia/alumina nanocomposite (Ce-TZP/AlO).

Prolactin selectively transported to cerebrospinal fluid from blood under hypoxic/ischemic conditions.

Aim: The aim of this study was to determine and to verify the correlation between the amount of prolactin (PRL) levels in the blood and in the cerebrospinal fluid (CSF) by various causes of death as an indicator for acute hypoxia in autopsy cases. It is to confirm the cause of the change in prolactin level in CSF by in vitro system.

Materials And Methods: In autopsy materials, the PRL levels in blood from the right heart ventricle and in the CSF were measured by chemiluminescent enzyme immunoassay, and changes in the percentage of PRL-positive cells in the pituitary gland were examined using an immunohistochemical method.

The lingual nerve: overview and new insights into anatomical variability based on fine dissection using human cadavers.

This article, for both researchers and clinicians, presents an overview of the lingual nerve and highlights how new insights into human anatomical variability can be gained by integrating fine dissection of cadavers with neuroanatomical approaches, microscopic studies, and morphometric techniques. Textbooks mainly provide descriptions of the typical or common gross anatomical appearance of structures in the human body with little reference to the nature and extent of variation that may be encountered within and between populations. Furthermore, few texts attempt to integrate descriptions of the regional distribution and branching of neural structures with their central connections or their microscopic anatomy.

Effect of the anteroposterior position of the model on fabricated mouthguard thickness: Part 2 Influence of sheet thickness and material.

Aim: Mouthguards can reduce the risk of sports-related injuries, but the sheet material and thickness have a large effect on their efficacy and safety. The aim of this study was to investigate the thickness of molded mouthguards when the model position on the forming table was changed stepwise in the anteroposterior direction for different sheet thicknesses and materials.

Materials And Methods: Ethylene vinyl acetate sheets and olefin copolymer sheets with 4.

Mouthguard sheet temperature after heating.

Background/aim: Mouthguard sheet materials such as ethylene vinyl acetate and polyolefin have been used commonly. However, the change of the sheet temperature during heating of the polyolefin has not been determined. The aim of this study was to examine the change of the sheet temperature during heating and to examine the vacuum-formed mouthguard characteristics for the sheet materials.

A Case of Right-Sided Ulcerative Colitis with Mesalamine-Induced Hypersensitivity Reactions.

BACKGROUND Ulcerative colitis (UC) is a chronic inflammatory bowel disease, affecting the colon continuously from the rectum proximally. However, a clinical type with right-sided colitis sparing the anal side of the colon is also known. Mesalamine, which is generally used to treat UC, can rarely aggravate the disease.