Black Rice Anthocyanins as An Effective Antioxidant of Inhibition of Oil Oxidative Based on Molecular Modification.

This present work investigated the influence of black rice anthocyanins as antioxidants on the oxidation stability of oil. Malonic acid, succinic acid and succinic anhydride were grafted on black rice anthocyanins through acylation method to improve their antioxidant activity in oil. The results from fourier transform infrared spectroscopy (FTIR) showed new absorption peaks near 1744 cm and 1514 cm , which implied that malonic acid, succinic acid and succinic anhydride grafted on the -OH of glucoside and rutinoside through esterification reaction and resulted that the polarity of these were reduced.

Effects of elevated levels of intracellular nitric oxide on Pseudomonas aeruginosa biofilm in chronic skin wound and slow-killing infection models.

Nitric oxide (NO), produced through the denitrification pathway, regulates biofilm dynamics through the quorum sensing system in Pseudomonas aeruginosa. NO stimulates P. aeruginosa biofilm dispersal by enhancing phosphodiesterase activity to decrease cyclic di-GMP levels.

Pan-cancer analysis of aldolase B gene as a novel prognostic biomarker for human cancers.

Aldolase B (ALDOB) gene is essential for the process of glycolysis and differentially expressed in cancers. The aims of this study were to explore the potential role of ALDOB in pan-cancer, in order to deepen the research on the pathological mechanism of cancer. Hence, we used several online tools (TIMER2, GEPIA2, UALCAN, cBioPortal, and MXPRESS) and R language to identify the correlation between the ALDOB expression and survival analysis, genetic alteration, DNA methylation, and immune cell infiltration based on The Cancer Genome Atlas project.

Structural Engineering of Red Luminogens to Realize High Emission Efficiency through ACQ-to-AIE Transformation.

Deep red/near-infrared (NIR, >650 nm) emissive organic luminophores with aggregation-induced emission (AIE) behaviours have emerged as promising candidates for applications in optoelectronic devices and biological fields. However, the molecular design philosophy for AIE luminogens (AIEgens) with narrow band gaps are rarely explored. Herein, we rationally designed two red organic luminophores, FITPA and FIMPA, by considering the enlargement of transition dipole moment in the charge-transfer state and the transformation from aggregation-caused quenching (ACQ) to AIE.

Betaine supplementation alleviates dextran sulfate sodium-induced colitis regulating the inflammatory response, enhancing the intestinal barrier, and altering gut microbiota.

Inflammatory bowel disease (IBD) is a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract. Betaine is a natural compound that exerts beneficial anti-inflammatory effects. However, the role of betaine in protecting IBD is still unclear.

A Genetic Toxicology Study of the Rapid Detection of Nitrosamine Compounds by the Gene Mutation Assay.

In a gene mutation experiment, the mutagenicity of the nitrosamine compounds -diethylnitrosamine (NDEA) and -dipropylnitrosamine (NDPA) was investigated at the cellular level, as well as with PCR (polymerase chain reaction) and RCA (rolling-circle amplification) amplification systems. The experiments were set up with 10 ppm, 100 ppm, and 1000 ppm concentration gradients of NDEA and NDPA, and ethidium bromide (EB) was used as a positive control group. The results demonstrated that the mutagenic frequency of NDEA and NDPA was significantly higher than the spontaneous mutation frequency of the gene under the same conditions, but lower than the mutagenic rate of EB in the positive control, and there was a dose-effect relationship, indicating that NDEA and NDPA could induce gene mutation.

Phosphate Starvation by Energy Metabolism Disturbance in Δ/Δ Induces Lipid Droplet Accumulation and Cell Membrane Damage.

Phosphorus in the form of phosphate (Pi) is an essential element for metabolic processes, including lipid metabolism. In yeast, the inositol polyphosphate kinase mediated synthesis of inositol heptakisphosphate (IP) regulates the phosphate-responsive (PHO) signaling pathway, which plays an important role in response to Pi stress. The role of in Pi stress and lipid metabolism of has not yet been studied.

Uptake, translocation, and subcellular distribution of three triazole pesticides in rice.

Triazole pesticides are widely used for the control of pathogenic fungi in crops, which were frequently detected in edible parts. Its extensive use has caused many environmental pollution and food safety problems. In this study, the uptake, translocation, and subcellular distribution of three triazole pesticides (triadimefon, tebuconazole, and epoxiconazole) in rice were investigated.

Transcriptional analysis and target genes discovery of Pseudomonas aeruginosa biofilm developed ex vivo chronic wound model.

Bacterial biofilms formation is one of the major reasons for treatment failure in chronic wound infections. Therefore, diagnostic biomarkers remain the best option for prevention and treatment of chronic wound infections by biofilms. Herein, Pseudomonas aeruginosa PAO1 was used to mimic biofilm development in porcine skin explants wells as ex vivo wound model.

Photothermal Responsive Singlet Oxygen Nanocarriers for Hypoxic Cancer Cell Ablation.

Hypoxia in aggressively proliferating tumor cells has been demonstrated to restrict the efficiency of photodynamic therapy owing to its oxygen (O )-dependent generation of singlet oxygen ( O ) from photosensitizers under light irradiation. To address this problem, we propose a small-molecule dye-based O capturing agent, B1. B1 not only bears a near-infrared absorbing azo-boron dipyrromethene backbone, but also has 1,4-dimethylnaphthalene, which facilitates the capture of O to form endoperoxide (B1-SO).

1-(4-Amino-2-Hydroxyphenyl)Ethenone Suppresses Virulence and Metabolism.

The impact of 1-(4-amino-2-hydroxyphenyl)ethanone (AHPE) from the metabolites of endophytic fungus on quorum sensing (QS) of was evaluated for the first time in this study. Exposure to AHPE at concentrations ranging from 12.5 to 50 μg/mL, the β-galactosidase activity, acyl-homoserine lactone level, swimming motility, chemotaxis, and flagella formation were significantly inhibited.

1-(4-Amino-2-hydroxyphenyl)ethanone from Phomopsis liquidambari showed quorum sensing inhibitory activity against Pseudomonas aeruginosa.

Phomopsis liquidambari S47 is an endophytic fungus isolated from the leaves of Punica granatum. Here, we are the first to report a quorum sensing (QS) inhibitor 1-(4-amino-2-hydroxyphenyl)ethanone (AHE) isolated and identified from the metabolites of P. liquidambari S47.

Effect of banana pulp dietary fibers on metabolic syndrome and gut microbiota diversity in high-fat diet mice.

Banana (Musa nana Lour.) have the effect of anti-obesity and lipid modulating properties. However, the influences of banana pulp dietary fibers (BP-DF) on metabolic syndrome (MetS) and gut microbiota (GM) are unknown.

Nanoparticle-Mediated Trapping of Wnt Family Member 5A in Tumor Microenvironments Enhances Immunotherapy for B-Raf Proto-Oncogene Mutant Melanoma.

Development of an effective treatment against advanced tumors remains a major challenge for cancer immunotherapy. Approximately 50% of human melanoma is driven by B-Raf proto-oncogene mutation (BRAF mutant). Tumors with such mutation are desmoplastic, highly immunosuppressive, and often resistant to immune checkpoint therapies.

BRAF peptide vaccine facilitates therapy of murine BRAF-mutant melanoma.

Approximately, 50% of human melanomas are driven by BRAF mutations, which produce tumors that are highly immunosuppressive and often resistant to vaccine therapy. We introduced lipid-coated calcium phosphate nanoparticles (LCP NPs) as a carrier to efficiently deliver a tumor-specific antigen, the BRAF peptide, to drive dendritic cell (DC) maturation and antigen presentation in C57BL6 mice. The BRAF peptide vaccine elicited a robust, antigen-specific cytotoxic T cell response and potent tumor growth inhibition in a murine BRAF-mutant melanoma model.

A new recombinant hybrid polypeptide and its immunologic adjuvant activity for inactivated infectious bursal disease vaccine.

Both bursin (Lys-His-Gly-NH2) and Gagnon's peptides (Lys-Asn-Pro-Tyr) can induce B-cell differentiation. However, it is unclear whether a recombinant hybrid polypeptide consisting of a tandem array of 14 copies of bursin and two copies of Gagnon's peptide can induce the proliferative activity of lymphocytes. Here, this recombinant hybrid polypeptide was expressed in Escherichia coli and purified by SDS-PAGE.