Inhibition, Gastritis Attenuation, and Gut Microbiota Protection in C57BL/6 Mice by NCUH062003.

(), one of the most prevalent pathogenic bacteria worldwide, is the leading cause of gastritis, gastric intestinal metaplasia, and gastric cancer. Antibiotics, the conventional treatment for eliminating , often lead to severe bacterial resistance, gut dysbiosis, and hepatic insufficiency and fail to address the inflammatory response or gastric mucosal damage caused by infection. In this study, based on 10-week animal experiments, two models of NCUH062003 for the prophylaxis and therapy of infection in C57BL/6 mice were established; a comprehensive comparative analysis was performed to investigate the anti- effect of probiotics, the reduction in inflammation, and repair of gastric mucosal damage.

The Catalytic Mechanism of Key Enzymes Involved in the Synthesis of Ergothioneine in .

C-S lyase is a crucial enzyme responsible for the formation of sulfur-containing flavor compounds in . We investigated the involvement of C-S lyase in the synthesis of ergothioneine (EGT) in , a high-producing edible mushroom. Through experimental and computational approaches, we identified 2, a C-S lyase, as a key enzyme involved in EGT synthesis in .

Different Treatments on the Physicochemical Properties and Volatile Components of Tea Wine During Storage Period.

Tea wine has garnered significant attention due to its unique fusion of tea and wine flavors, as well as its alleged health benefits. This study aimed to investigate the effects of various treatments on the physicochemical properties of tea wine, including viable cell counts, pH, acidity, total ester content, tea polyphenol content, and volatile flavoring substances during the storage period. The findings indicated that tea wine subjected to low-temperature plasma (LTPS) treatment exhibited superior quality maintenance and an enhanced tea polyphenol content compared to untreated, UV-treated, and HTHP-treated tea wine.

Adaptive Laboratory Evolution of Flavin Functionality Identifies Dihydrolipoyl Dehydrogenase as One of the Critical Points for the Activity of 7,8-Didemethyl-Riboflavin as a Surrogate for Riboflavin in .

Riboflavin analogs lacking one methyl group (7α or 8α) can still serve as a surrogate for riboflavin in riboflavin-deficient microorganisms or animals. The absence of both methyl groups at once completely abolishes this substitution capability. To elucidate the molecular mechanisms behind this phenomenon, we performed an adaptive laboratory evolution experiment (in triplicate) on an strain auxotrophic for riboflavin.

Scanning Electron Microscopy of the Proboscis and Associated Sensilla in (Esper, 1805) (Lepidoptera: Pieridae).

Morphological studies of the proboscis can provide valuable insights into the evolutionary adaptations of Lepidoptera. However, such research is relatively limited for Pieridae, a family that is significant both ecologically and economically. In this study, scanning electron microscopy (SEM) was employed to investigate the proboscis and associated sensilla of adult , with an emphasis on ultrastructural details and potential sex-specific differences.

Recent Progress of Molecularly Imprinted Technique for the Detection of Mycotoxins in Food.

Mycotoxins are a group of toxic metabolites produced by fungi that infect agricultural products. Consuming mycotoxin-contaminated foods and feeds can cause various adverse health effects in humans and animals. Therefore, developing rapid and sensitive analytical methods for detecting mycotoxins is an urgent task.

Effects of L9 on Oral Microbiota and Cariogenic Factors in -Infected Mice.

In the pathogenesis of dental caries, () plays a central role. can produce extracellular polysaccharides, which can help the bacteria form biofilms on the tooth surface, create a stable living environment, and hinder the removal of bacteria by natural defense substances in the oral cavity such as saliva. Meanwhile, the oral microbiota and dietary habits exert long-term influences on its development.

Effects of Lactic Acid Bacteria Fermentation and In Vitro Simulated Digestion on the Bioactivities of Purple Sweet Potato Juice.

The effects of lactic acid bacteria fermentation and in vitro simulated digestion on phenolic bioavailability, phenolic bioavailability, and antioxidant activity of purple sweet potato juice (PSPJ) were investigated. The PSPJ was fermented by and . The viable bacterial count, phenolic components, antioxidant activity, phenolic bioaccessibility, and phenolic bioavailability of PSPJ were analyzed during the simulated digestion process in vitro.

Quality Characteristics and Flavor Analysis of Five Mulberry Varieties.

For a deeper understanding of the characteristics exhibited by several novel mulberry varieties, the quality attributes and flavor components of five mulberry varieties (Zhongsang 5801, 2000-3, Jialing 40, Yuesang 10, and White Shahtoot Mulberry) were analyzed and compared. Zhongsang 5801 displayed the highest total phenol and flavone levels. Fructose and glucose were the primary sugars identified in the mulberries, with 2000-3 exhibiting the highest fructose content (39.

A Comprehensive Review of Food Safety Culture in the Food Industry: Leadership, Organizational Commitment, and Multicultural Dynamics.

Food safety culture (FSC) has transitioned from a narrow compliance-based concept to a comprehensive organisational value that is essential for ensuring food safety. This review explores the pivotal roles of leadership, organisational commitment, and cultural diversity in shaping an effective FSC. It highlights how leadership style, particularly transformational leadership, can enhance employee engagement and foster a proactive safety culture.

Effects of Pretreatment Methods on Gamma-Aminobutyric Acid Enrichment and Quality Improvement in Highland Barley Beverages.

Gamma-aminobutyric acid (GABA) is an important neurotransmitter that promotes sleep and reduces anxiety, but its natural synthesis in the body is insufficient, necessitating dietary intake. This study utilized a combination of germination, the addition of active barley powder, and fermentation to enhance GABA content in an enzymatically hydrolyzed highland barley beverage. The samples were divided into five groups: highland barley (HB), germinated highland barley (GB), highland barley supplemented with another high-glutamic-acid decarboxylase-active highland barley powder TB13 (BT), germinated barley supplemented with TB13 (GBT), and germinated barley supplemented with TB13 followed by fermentation (GBTF).

Strategies and Methodologies for Improving Toughness of Starch Films.

Starch films have attracted increasing attention due to their biodegradability, edibility, and potential use as animal feed from post-products. Applications of starch-based films include food packaging, coating, and medicine capsules. However, a major drawback of starch-based films is their brittleness, particularly under dry conditions, caused by starch retrogradation and the instability of plasticizers.

Ultrasound-Assisted Extraction of Squalene and 2-Acetyl-1-Pyrroline from Pandan Leaf: The Effects of Drying Methods and Extraction Conditions.

Pandan, a tropical crop, is rich in squalene (SQ), known for its antioxidant and hypoglycemic properties, and 2-acetyl-1-pyrroline (2-AP), which imparts a characteristic aroma. This study focuses on the extraction of the two bioactive compounds from Pandan leaves and investigates the effects of drying methods, extraction solvents, and conditions on the yield of SQ and 2-AP. Results show that hot air-dried Pandan leaves when extracted using the binary solvent system of ethanol and n-hexane (EH), yield higher SQ content while maintaining an adequate content of 2-AP.

Effect of Heat Treatment on Gelatin Properties and the Construction of High Internal Phase Emulsions for 3D Printing.

The effect of tilapia skin gelatin properties on the characteristics of high internal phase emulsions (HIPEs) and the quality of 3D printing remains unidentified. In this work, HIPEs were constructed by gelatin with various properties that were obtained by heat treatment. The results indicated that the gelatin undergoes degradation gradually with an increase in heating intensity.

Anticoagulant Activity of the Polysaccharide Fromgonad of Abalone Ino: The Role of Conjugate Protein.

Few studies are concerned with the effect of the conjugat protein on the bioactivities of the abalone gonad polysaccharide (AGP). In this study, a series of treatments, including raw material (female and male) defatting, extraction temperature (25-121 °C), proteolysis, ultrafiltration, and ethanol precipitation, was conducted to investigate the role of the conjugate protein on AGP anticoagulant activity. All AGP extracts significantly prolonged activated partial thromboplastin time (APTT) and thrombin time (TT).

Applications of Near-Infrared Spectroscopy for Nondestructive Quality Analysis of Fish and Fishery Products.

Fish has become one of the most popular aquatic products for its beneficial effects. The quality of fish and fishery products may be influenced by their geographical origin, transportation, processing, and storage conditions. The availability of rapid and reliable techniques is important for nondestructive determination of their quality.

Developing a Simple and Feasible Process for the Crude Extraction of Livetins and Phosvitin from Egg Yolk.

Due to imbalanced demand favoring egg whites, the egg industry faces a surplus of egg yolk, limiting overall growth. This study designed a feasible process for the crude extraction of livetins and phosvitin (PV) and revealed the related separation mechanisms. Our method utilized a 1:9 egg yolk dilution at pH 6.

Study on the Improvement of Quality Characteristics of Pickles During Fermentation and Storage.

This study investigated the effect of fermentation-promoting peptides (FPPs) on the improvement of the quality of cowpea pickles during fermentation and storage. FPPs were introduced to evaluate their effects on key parameters such as pH, total acidity, nitrite levels, and salinity. FPP accelerated fermentation by stimulating lactic acid bacteria (LAB) activity, leading to a rapid reduction in pH and a stable increase in total acidity.

Impact of Cooking on Starch Digestibility in Foxtail Millets.

While the digestibility of millet starch has been studied considerably, the effects of cooking on starch digestibility in millet remain insufficiently understood. This study investigated the effects of cooking on in vitro enzymatic starch digestion in eight cooked millet flour cultivars by seeking its correlations with the changes in composition (moisture, total starch, protein, lipids, total dietary fiber, and phenolics), structure, and physicochemical properties. Compared to raw flours, cooked flours had a similar content of total starch and protein, a lower content of moisture, lipids, and total phenolic content, and a higher content of total dietary fiber.

One-Pot Synthesis of β-Alanine from Fumaric Acid via an Efficient Dual-Enzyme Cascade Biotransformation.

As the only naturally occurring β-amino acid, β-alanine has important application prospects in many fields. Driven by the huge demand, biosynthesis is becoming more and more popular as a potential alternative to the chemical synthesis of β-alanine. Although the direct pathway from L-aspartic acid to β-alanine, catalyzed by L-aspartic acid-α-decarboxylase (PanD), is ideal for β-alanine synthesis, it is hindered by the high cost of the substrate and limited economic viability.

Analysis of the Preventive Effect of Pomace and Its Isolated Components on Colitis in Mice Based on Gut Microbiota and Serum Metabolomics.

Inflammatory bowel disease (IBD), including relapsing-remitting ulcerative colitis and Crohn's disease, is a non-specific chronic intestinal inflammatory disease. , which is rich in polyphenolic compounds, has been shown to exert antioxidative and anti-inflammatory effects. The research evaluates the dietary impacts of pomace, its polyphenol-rich extract, and fiber-rich residue on colitis symptoms.

Preparation, structural changes and functional properties of the covalent complexes of almond protein and phloretin.

Proteins and polyphenols exhibit distinct biological activities and functional properties. A comprehensive investigation into the formation mechanisms, structures, and functional properties of protein-polyphenol complexes will deepen our understanding of their interactions and establish a theoretical foundation and technical support for development of novel functional foods and pharmaceutical products. The almond protein-phloretin (AP-PHL) covalent complex was synthesized through the covalent binding of hydroxyl radicals to phloretin (PHL), utilizing almond protein (AP) as the raw material.

Microbial perspective of multidisciplinary collaborative weight management approach: may serve as a key target for weight loss.

Changes in the gut microbiota are associated with obesity and may influence weight loss. We are currently implementing a sustained multidisciplinary collaborative weight management (MCWM) approach to weight loss. We report significant improvements in participant health status after 6 months, along with alterations in the structure, interactions, and metabolic functions of the microbiota.

Physicochemical characterization, bioactive compounds, and in vitro digestion characteristics of Moringa oleifera seed oil: a comprehensive investigation.

Background: Moringa oleifera is a wild plant belonging to the genus Moringa and the family Moringaceae, which possesses valuable nutritional and medicinal properties and is inexpensive. The present study aimed to provide a comprehensive assessment of the potential of M. oleifera seed oil (MoSO) as a food ingredient by investigating its physicochemical properties, bioactivity, and in vitro digestion characteristics.

Exploring the Therapeutic Potentials and Molecular Mechanisms of Alkaloids in Ulcerative Colitis: An Integrative Network Pharmacology and Molecular Docking Approach.

, a medicinal plant traditionally used in Southeast Asia, exerts protective effects against various inflammatory diseases, primarily due to its rich alkaloid content. Despite substantial evidence supporting its anti-inflammatory properties, the biological activities of are unclear. This study aimed to elucidate anticolitis mechanisms of alkaloids (CFAs) using an integrative approach of network pharmacology and molecular docking analyses.