Common positioning errors in panoramic radiography: A review.

Professionals performing radiographic examinations are responsible for maintaining optimal image quality for accurate diagnoses. These professionals must competently execute techniques such as film manipulation and processing to minimize patient exposure to radiation. Improper performance by the professional and/or patient may result in a radiographic image of unsatisfactory quality that can also lead to a misdiagnosis and the development of an inadequate treatment plan.

Analysis of surgical margins in oral cancer using in situ fluorescence spectroscopy.

Unlabelled: Oral cancer is a public health problem with high prevalence in the population. Local tumor control is best achieved by complete surgical resection with adequate margins. A disease-free surgical margin correlates with a lower rate of local recurrence and a higher rate of disease-free survival.

The fatty acid synthase inhibitor orlistat reduces the growth and metastasis of orthotopic tongue oral squamous cell carcinomas.

Fatty acid synthase (FASN) is the biosynthetic enzyme responsible for the endogenous synthesis of fatty acids. It is downregulated in most normal cells, except in lipogenic tissues such as liver, lactating breast, fetal lung, and adipose tissue. Conversely, several human cancers, including head and neck squamous cell carcinomas (HNSCC), overexpress FASN, which has been associated with poor prognosis and recently suggested as a metabolic oncoprotein.

Evaluation of condyle defects using different reconstruction protocols of cone-beam computed tomography.

This study was conducted to investigate how well cone-beam computed tomography (CBCT) can detect simulated cavitary defects in condyles, and to test the influence of the reconstruction protocols. Defects were created with spherical diamond burs (numbers 1013, 1016, 3017) in superior and/or posterior surfaces of twenty condyles. The condyles were scanned, and cross-sectional reconstructions were performed with nine different protocols, based on slice thickness (0.

Evaluation of the radiopacity of esthetic root canal posts.

Unlabelled: The radiopacity of esthetic root canal posts may impair the assessment of their fit to the root canal when using radiographic images. This study determined in vitro the radiographic density of esthetic root canal posts using digital images. Thirty-six roots of human maxillary canines were assigned to six groups (N=6 per group): Reforpost (RP); Aestheti-Plus (AP); Reforpost MIX (RPM); D.

Biomechanical strength analysis of mini anchors for the temporomandibular joint.

Introduction: The usage of mini anchors for the treatment of internal derangements of the temporomandibular joint (TMJ) is considered an innovative treatment, and it has presented excellent clinical results in the stabilization of the articular disc. This study aimed to evaluate, through mechanical tensile testing, the resistance of mini anchors for TMJ articular disc repositioning.

Materials And Methods: Ten thermoplastic polymer poly-ether-ether-ketone (PEEK) mini anchors were tested in artificial polyurethane blocks with a pullout test in a mechanical Instron™ machine, model 4411.

Bioactive Fraction of Geopropolis from Melipona scutellaris Decreases Neutrophils Migration in the Inflammatory Process: Involvement of Nitric Oxide Pathway.

The aim of this study was to evaluate the activity of the ethanolic extract of geopropolis (EEGP) from Melipona scutellaris and its fractions on the modulation of neutrophil migration in the inflammatory process, and the participation of nitric oxide (NO) pathway, as well as to check the chemical profile of the bioactive fraction. EEGP and its aqueous fraction decreased neutrophil migration in the peritoneal cavity and also the interaction of leukocytes (rolling and adhesion) with endothelial cells. The levels of chemokines CXCL1/KC and CXCL2/MIP-2 were not altered after treatment with EEGP and the aqueous fraction.

Salivary carriage of periodontal pathogens in generalized aggressive periodontitis families.

Background: Generalized aggressive periodontitis (GAP) is a multifactorial disease that shows a specific microbial profile and a familial aggregation.

Aim: This study evaluated the salivary microbial profile of families with a history of GAP and compared them with healthy families.

Design: Fifteen families with parents presenting periodontal health and 15 with parents with a history of GAP were selected.

The fatty acid synthase inhibitor orlistat reduces experimental metastases and angiogenesis in B16-F10 melanomas.

Background: Fatty acid synthase (FASN) is overexpressed and associated with poor prognosis in several human cancers. Here, we investigate the effect of FASN inhibitors on the metastatic spread and angiogenesis in experimental melanomas and cultured melanoma cells.

Methods: The lung colonisation assay and cutaneous melanomas were performed by the inoculation of mouse melanoma B16-F10 cells in C57BL6 mice.

Geopropolis from Melipona scutellaris decreases the mechanical inflammatory hypernociception by inhibiting the production of IL-1β and TNF-α.

Ethnopharmacological Relevance: The pharmacological activity of geopropolis collected by stingless bees (important and threatened pollinators), a product widely used in folk medicine by several communities in Brazil, especially in the Northeast Region, needs to be studied.

Objective: The aim of this study was to evaluate the antinociceptive activity of Melipona scutellaris geopropolis (stingless bee) using different models of nociception.

Material And Methods: The antinociceptive activity of the ethanolic extract of geopropolis (EEGP) and fractions was evaluated using writhing induced by acetic acid, formalin test, carrageenan-induced hypernociception, and quantification of IL-1β and TNF-α.

Factors associated with the prevalence of periodontal disease in low-risk pregnant women.

Objective: To evaluate the prevalence of periodontal disease (PD) among Brazilian low-risk pregnant women and its association with sociodemographic factors, habits and oral hygiene.

Method: This cross-sectional study included 334 low-risk pregnant women divided in groups with or without PD. Indexes of plaque and gingival bleeding on probing, probing pocket depth, clinical attachment level and gingival recession were evaluated at one periodontal examination below 32 weeks of gestation.

Influence of endodontic irrigants on bond strength of a self-etching adhesive.

The aim of this in vitro study was to evaluate the bond strength of a self-etching adhesive system to dentine irrigated with sodium hypochlorite (NaOCl), chlorhexidine solution (CX) and ethylene diamine tetra acetic acid (EDTA) by microtensile testing. Sixty human third molars were sectioned 3 mm below the occlusal level and then randomly divided into six groups: G1(control), without irrigant solution; G2, 1% NaOCl; G3, 1% NaOCl followed by the application of 17% EDTA; G4, 2% CX; G5, 2% CX followed by the application of 17% EDTA and G6, 17% EDTA. The specimens received the self-etching adhesive system were restored with composite resin, then sectioned and trimmed to obtain four hourglass-shaped slabs from each tooth.

Clinicopathological factors are predictors of distant metastasis from major salivary gland carcinomas.

The risk of distant metastasis of salivary gland cancers has usually been associated with histological type, tumour size, and site. The aim of this study was to evaluate a series of patients with major salivary gland carcinomas in order to identify potential risk factors associated with distant metastasis. 255 patients treated for major salivary gland carcinoma in Brazil from 1953 to 2004 were reviewed.

Assessment of the tip surface of gutta-percha cones after different cutting methods.

The surface of gutta-percha cones was evaluated after using five different cutting methods, including a new TipSnip device. The gutta-percha cones were cut off using: 1) TipSnip, 2) a single cut with a scalpel blade using a gauge, 3) two cuts with a scalpel blade using a gauge, 4) a razor blade against a glass slab or 5) scissors. Samples were examined under stereomicroscopy and observed by three highly qualified evaluators.

PLUNC protein expression in major salivary glands of HIV-infected patients.

Objective: To analyse and compare the expression of Palate, Lung, and Nasal Epithelium Clone (PLUNC) proteins in salivary glands from patients with and without AIDS (control group) using autopsy material.

Methods: We analysed the expression of PLUNCs using immunohistochemistry in parotid (n = 45), submandibular (n = 47) and sublingual gland (n = 37) samples of AIDS patients [30 with normal histology, 21 with mycobacteriosis, 14 with cytomegalovirus (CMV) infection, 30 with chronic non-specific sialadenitis, and 30 HIV-negative controls. In situ hybridization (ISH) for SPLUNC 2 in the HIV-negative group was performed.

DNA methylation status of the IL8 gene promoter in oral cells of smokers and non-smokers with chronic periodontitis.

Aim: This study analysed the status of DNA methylation in the promoter region of the IL8 gene in oral mucosa cells from healthy, smoker and non-smoker subjects with chronic periodontitis and compared these findings among groups with mRNA levels.

Material And Methods: Genomic DNA from epithelial oral cells of 41 healthy subjects, 30 smokers with chronic periodontitis and 40 non-smokers with chronic periodontitis were purified and modified by sodium bisulphite. Genomic DNA from blood leucocytes and gingival cells from biopsies of 13 subjects of each group were also purified and modified by sodium bisulphite.

Oral traumatic neuroma with mature ganglion cells: A case report and review of the literature.

Traumatic neuromas are characterized by the presence of pain, burning, or paresthesia, associated with a history of trauma, normally surgery, in the same site. In the oral cavity, the most commonly affected sites are the lip, tongue, and mental nerve area. Pressure on the suspected area usually provokes pain.

Clinicopathological significance of ubiquitin-specific protease 2a (USP2a), fatty acid synthase (FASN), and ErbB2 expression in oral squamous cell carcinomas.

Overexpression of fatty acid synthase (FASN) and ErbB2 has been described in oral squamous cell carcinomas (OSCC). FASN is the key lipogenic enzyme responsible for the endogenous synthesis of fatty acids and its expression can be regulated by ErbB2. The deubiquitinating enzyme (DUB) ubiquitin-specific protease 2a (USP2a) plays a critical role in prostate cancer cell survival by stabilizing the FASN protein.

Fatty acid synthase inhibition with Orlistat promotes apoptosis and reduces cell growth and lymph node metastasis in a mouse melanoma model.

Fatty acid synthase (FASN) is the enzyme responsible for the endogenous synthesis of the saturated fatty acid palmitate. In contrast to most normal cells, malignant cells depend on FASN activity for growth and survival. In fact, FASN is overexpressed in a variety of human cancers including cutaneous melanoma, in which its levels of expression are associated with a poor prognosis and depth of invasion.

Efficacy of denture cleansers on denture liners contaminated with Candida species.

As poor denture hygiene is related to Candida colonisation, disinfectant solutions have been proposed as an effective method of preventing denture stomatitis. This study assessed the efficacy of denture cleansers on Candida albicans and Candida glabrata adherence on denture liners. Another aim was to correlate materials' surface roughness (Ra) to Candida adherence.

Effect of incisal tooth wear and restoration on interocclusal distance during Brazilian Portuguese language speech.

Aim: The aim of this study was to investigate changes in interocclusal distance (IOD) during pronunciation of /m/ and /s/ sounds in Brazilian Portuguese, in patients presenting dental wear due to bruxism, before and after placement of a stabilization appliance and restorative treatment.

Methods: Subjects were divided into a control group of 19 patients with no dental wear and an experimental group of 18 patients presenting dental wear on anterior teeth due to bruxism. A stabilization appliance was placed in each patient in the experimental group and anterior teeth were restored.

Comparison of transcranial radiograph and magnetic resonance imaging in the evaluation of mandibular condyle position.

Objectives: Several image modalities have been used to assess the condylar position in the glenoid fossa. However, despite the development of more advanced techniques for imaging the temporomandibular joint (TMJ), the transcranial projection remains widely used in dentistry. The purpose of this study was to compare the condylar position in transcranial radiography (TRANS) with MRI.

Differential expression of fatty acid synthase (FAS) and ErbB2 in nonmalignant and malignant oral keratinocytes.

The aim of this study was to investigate fatty acid synthase (FAS) and ErbB2 expression in nonmalignant oral epithelium and oral or head and neck squamous cell carcinomas (OSCC/HNSCC). Morphologically normal, hyperkeratotic, and dysplastic oral epithelium as well as well-differentiated and poorly differentiated OSCC were immunohistochemically evaluated for FAS, ErbB2, and Ki-67. These proteins were also analyzed in a tissue microarray with 55 HNSCC.

The prevalence of bifid mandibular condyle detected in a Brazilian population.

Objectives: To determine the prevalence of bifid mandibular condyle (BMC) detected on panoramic radiographs in a Brazilian population.

Methods: The panoramic radiographs of 50 080 radiographs taken from 1999 to 2006 were retrospectively reviewed in order to identify BMC cases.

Results: 9 (0.