The combination of RL-QN15 and OH-CATH30 to promotes the repair of acne via the TLR2/NF-κB pathway.

Acne is a prevalent and chronic inflammatory skin disease, and its treatment remains a huge clinical challenge. In the present study, we evaluated the therapeutic potential of combining the peptides RL-QN15 and OH-CATH30 for the treatment of acne in mice. Results indicated that the topical application of RL-QN15 and OH-CATH30 significantly inhibited the proliferation of Propionibacterium acnes (P.

Exploring the Role of Exosomal lncRNA in Cancer Immunopathogenesis: Unraveling the Immune Response and EMT Pathways.

Exosomes are membrane-bound vesicles secreted by diverse cell types, serving as crucial mediators in intercellular communication and significantly influencing cancer development. Exosomes facilitate complex signaling processes in the tumor microenvironment for immunomodulation, metastasis, angiogenesis, and treatment resistance. Notably, long non-coding RNAs (lncRNAs), a class of non-coding RNAs, engage with mRNA, DNA, proteins, and miRNAs to modulate gene expression through multiple mechanisms, including transcriptional, post-transcriptional, translational, and epigenetic pathways.

Lignin-based nano-mimetic enzymes: A promising approach for wastewater remediation.

Lignin-based nano-mimetic enzymes have emerged as a promising approach for wastewater remediation, addressing the limitations of conventional treatment methods. This review article explores the potential of lignin, a renewable biomaterial, in developing these novel enzyme-inspired systems. The introduction highlights the rising pollution levels, stricter environmental regulations, and the need for innovative wastewater treatment technologies.

Anti-angiogenic activity and mechanism of fucoidan from brown algae, Sargassum Naozhouense mediated by intracellular antioxidation.

Fucoidan has various physiological activities, and its structure is also different according to different brown algae. In this study SNF (Sargassum Naozhouense fucoidan) was extracted by acid extraction method, and its relative molecular weight was determined to be 631.40 kDa.

Clickable liposomes for on-demand reversal of antiplatelet drugs: Towards a safe management of bleeding risks associated with antithrombotic therapy.

Antithrombotic drugs are widely used to prevent thrombotic events in patients with cardiovascular diseases. However, they all carry varying degrees of bleeding risk. Currently, there are no approved reversal agents for antiplatelet medications, which limits their further clinical application and poses challenges in managing bleeding complications.

Structural insights into the biochemical mechanism of the E2/E3 hybrid enzyme UBE2O.

The E2/E3 hybrid enzyme UBE2O plays important roles in key biological events, but its autoubiquitination mechanism remains largely unclear. In this study, we determined the crystal structures of full-length (FL) UBE2O from Trametes pubescens (tp) and its ubiquitin-conjugating (UBC) domain. The dimeric FL-tpUBE2O structure revealed interdomain interactions between the conserved regions (CR1-CR2) and UBC.

Calcineurin: An essential regulator of sleep revealed by biochemical, chemical biological, and genetic approaches.

Research into mechanisms underlying sleep traditionally relies on electrophysiology and genetics. Because sleep can only be measured on whole animals by behavioral observations and physical means, no sleep research was initiated by biochemical and chemical biological approaches. We used phosphorylation sites of kinases important for sleep as targets for biochemical and chemical biological approaches.

Mechanistic modeling of anti-Langmuirian to Langmuirian behavior of Fc-fusion proteins in cation exchange chromatography.

Development of a next-generation chromatographic model, capable of simultaneously meeting academic demands for thermodynamic consistency and industrial requirements in everyday project work, has become a focal point of research. In this study, anti-Langmuirian to Langmuirian (AL-L) elution behavior was observed in cation-exchange chromatographic separation of charge variants of industrial Fc-fusion proteins. To characterize this behavior, the multi-protein Mollerup activity model was integrated into the steric mass action (SMA) model, resulting in a new model named the generalized ion-exchange (nGIEX) isotherm for multi-protein systems.

Ligation-recognition triggered RPA-Cas12a cis-cleavage fluorogenic RNA aptamer for one-pot and label-free detection of MicroRNA in breast cancer.

"One-pot" assays which combine amplification with CRISPR/Cas12a system are in constant attracted for biosensors development. Herein, we present a one-pot isothermal assay that Ligation-recognition triggered Recombinase Polymerase Amplification (RPA)-CRISPR/Cas12a cis-cleavage (LRPA-CRISPR) fluorescent biosensor for sensitive, specific, and label-free miRNA detection. Firstly, we reveal the programmed double-stranded DNA amplicons, which utilized the ligation-recognition and polymerization to form and amplified by the RPA system.

A bifunctional coumarin-based CD probe for chiral analysis of amino acids in aqueous solution.

Amino acids play important roles in human pathology and physiology and the qualitative and quantitative determination of chiral amino acids in humans and mammals also has important impacts on the life sciences. Therefore, the introduction of artificial probes to assess the concentrations and enantiomeric compositions [ee = ([D] - [L])/([D] + [L])] of amino acids in aqueous solution is necessary in understanding certain biological processes and diagnosing and treating diseases. Herein, a bifunctional achiral coumarin probe (Br-Coumarin) is reported to determine the absolute configuration, ee value, and concentration of 16 amino acids in THF/HO = 1/4 solution at micromolar concentrations.

Near-infrared fluorescence imaging platform with ultra large Stokes shift for monitoring and bioimaging of hydrogen peroxide in the process of ferroptosis.

Hydrogen peroxide (HO), as a strong oxidant, is crucial for the aerobic metabolism of organisms and is intricately linked to the onset of numerous diseases. Real-time monitor HO levels in the environment and biological microenvironment is of paramount importance for environment protection and elucidating HO-related physiological and pathological processes. In this study, a novel near-infrared fluorescence imaging platform was developed and a near-infrared fluorescent probe FBMH was constructed based on the platform with photoinduced electron transfer mechanism.

Mapping the local ambidextrous chirality in thin films of N phase by circular dichroism spectra.

Circular dichroism mapping (CDM) method was introduced by utilizing the highly collimated light beam of synchrotron radiation (SR) available at Diamond Light Source B23 beamline for scanning the thin films of the N phase. We apply SR-CDM to two achiral dimeric materials exhibiting the N phase: symmetric DTC5C9 and dissymmetric DTC5C9CB. The SR-CDM measurements directly capture the chiral information in the local N domains, providing the ultimate complement to the theoretical predictions of the helical structures: the spontaneous symmetry breaking in N phase is ambidextrous.

Unsymmetric hydroxylamine and hydrazine BAM15 derivatives as potent mitochondrial uncouplers.

Chemical mitochondrial uncouplers are protonophoric, lipophilic small molecules that transport protons from the mitochondrial intermembrane space into the matrix independent of ATP synthase, thus uncoupling nutrient oxidation from ATP production. Our previous work identified BAM15 (IC 0.27 μM) as a potent and efficacious mitochondrial uncoupler with potential for obesity treatment.

Bimetal-organic framework-templated Zn-Fe-based transition metal oxide composites through heterostructure optimization to boost lithium storage.

Transition metal oxides (TMOs), especially zinc- and iron-based materials, are known to be one of the most innovative anode materials based on their high theoretical capacity, low price and abundant natural reserves. However, the application of these materials is limited by poor electronic conductivity, slow ion mobility and large structural transformations during charging/discharging processes. To overcome these drawbacks, sacrificial template technology has been proposed as a promising strategy to optimize the electrochemical performance and structure stability of TMOs, showing its potential especially in the storage design of lithium-ion batteries (LIBs).

In situ HO production from self-sufficient heterogeneous Fenton reaction over Fe/MoS for potential environmental remediation applications.

Fenton reaction technology has worked well in water and wastewater treatment; however it is often limited by such problems as continuous external supply of HO, slow Fe/Fe cycle rate, high energy requirements, and maintenance of low pH during operation. Herein, a novel self-sufficient heterogeneous Fenton system based on Fe/MoS was designed, fabricated, and optimized to effectively address these problems. The combined presence of Fe and sulfur vacancies sites in MoS played a pivotal role in the generation of HOvia two-step single-electron reduction process without any energy consumption.

Mesoporous polydopamine composite nanoparticles for multimodal therapy based on disrupting the redox homeostasis within tumor cells.

Developing multimodal combination therapy strategies to disrupt the redox homeostasis within tumor cells is currently an important approach in cancer treatment. In this study, we designed and prepared multifunctional composite nanoparticles MPDA-PEG@MnO@2-DG (MPPMD NPs) utilizing mesoporous polydopamine nanoparticles (MPDA NPs) as carriers. These carriers were coated with polyethylene glycol (PEG), and manganese dioxide (MnO) and loaded with 2-deoxy-d-glucose (2-DG).

Construction of crystalline/amorphous NiP/FePO/graphene heterostructure by microwave irradiation for efficient oxygen evolution.

The rational design of highly efficient and cost-effective oxygen evolution reaction (OER) electrocatalysts is crucial for hydrogen production through electrocatalytic water splitting. Although the crystalline/amorphous heterostructure shows great potential in enhancing OER activity, its fabrication presents significantly greater challenges compared to that of crystalline/crystalline heterostructures. Herein, a microwave irradiation strategy is developed to construct reduced graphene oxide supported crystalline NiP/amorphous FePO heterostructure (NiP/FePO/RGO) as an efficient OER electrocatalyst.

Self-assembly of defined core-shell ellipsoidal particles at liquid interfaces.

Hypothesis: Ellipsoidal particles confined at liquid interfaces exhibit complex self-assembly due to quadrupolar capillary interactions, favouring either tip-to-tip or side-to-side configurations. However, predicting and controlling which structure forms remains challenging. We hypothesize that introducing a polymer-based soft shell around the particles will modulate these capillary interactions, providing a means to tune the preferred self-assembly configuration based on particle geometry and shell properties.

Design of cerium dioxide anchored in cobalt-iron layered double hydroxide hollow polyhedra via an ion exchange strategy for the oxygen evolution reaction.

The oxygen evolution reaction (OER) is hindered by slow kinetics due to its four-electron process, limiting overall efficiency. The rational design of metal-organic framework (MOF)-based nanomaterials is crucial for enhancing the oxygen production rate. Using a straightforward strategy, we synthesized cobalt-iron layered double hydroxide (CoFe-LDH) hollow polyhedra loaded with CeO, with zeolite imidazolate framework-67 (ZIF-67) serving as the precursor.

Comprehensive analysis of transplacental transfer of environmental pollutants detected in paired maternal and cord serums.

Prenatal exposure to hazardous environmental pollutants is a critical global concern due to their confirmed presence in umbilical cord blood, indicating the ability of pollutants to cross the placental barrier and expose the fetus to harmful compounds. However, the transplacental transfer efficiencies (TTEs) of many pollutants remain underexplored. Herein, we developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantitatively analyze 91 environmental pollutants, including 13 bisphenols (BPs), 18 organophosphorus flame retardants (OPFRs), 7 brominated and other flame retardants (BFRs), 34 phthalates (PAEs), and 19 per- and polyfluoroalkyl substances (PFASs), in paired maternal and cord serums.

Soil carbon fractionation as a tool to monitor coastal wetland rehabilitation.

Coastal wetland rehabilitation can provide nature-based solutions for climate change mitigation. The high carbon accumulation rate and carbon secured, potentially for several millennia, as soil organic carbon (SOC), is among the reasons. Measuring SOC storage and accrual over time are the main tools to understand rehabilitation success.

Highly sensitive aggregation-induced electrochemiluminescence sensor for cadmium detection in Ganoderma lucidum.

Cadmium (Cd) pollution poses a major threat to food safety. Sensitive detection of Cd is of great significance for the life health. Herein, an aggregation-induced electrochemiluminescence (AIECL) sensor based on polymer dots (Pdots) is designed for Cd detection.

Illudin S inhibits p53-Mdm2 interaction for anticancer efficacy in colorectal cancer.

The impairment of the p53 pathway was once regarded as inadequately druggable due to the specificity of the p53 structure, its flat surface lacking an ideal drug-binding site, and the difficulty in reinstating p53 function. However, renewed interest in p53-based therapies has emerged, with promising approaches targeting p53 and ongoing clinical trials investigating p53-based treatments across various cancers. Despite significant progress in p53-targeted therapies, challenges persist in identifying effective therapeutic targets within the p53 pathway.

Mechanistic insights into the anti-oxidative and anti-inflammatory functions of covalent-reactive cinnamyl compounds within Cinnamomum cassia.

Background: Cinnamomum cassia Presl (Lauraceae) is widely used as a medicinal plant in the folk medicine and pharmaceutic industry, for its promising anti-inflammatory, anti-oxidative, and anti-bacterial function. However, the major bioactive components were still in debate, and their underlying molecular mechanism was not yet fully understood.

Purpose: This study aimed to identify the bioactive ingredients of C.