Population Structure and Regeneration Status of Woody Plant Species in Tulu Korma Dry Afromontane Forest, West Shewa Zone, Oromia, Ethiopia.

A study was conducted on afromontane forest to assess woody plant species' population structure and natural regeneration status. Data were collected from 52 main quadrats of 400 m for mature woody species and 260 subplots of 25 m for seedlings and saplings. All live woody plant species were recorded with their densities, heights, and DBH.

Response to Comments on "Metabolic scaling is the product of life-history optimization".

Froese and Pauly argue that our model is contradicted by the observation that fish reproduce before their growth rate decreases. Kearney and Jusup show that our model incompletely describes growth and reproduction for some species. Here we discuss the costs of reproduction, the relationship between reproduction and growth, and propose tests of models based on optimality and constraint.

Serological Cross-Reaction between Six Thiadiazine by Indirect ELISA Test and Their Antimicrobial Activity.

Malaria is a parasitic infection caused by a protozoon of the genus , transmitted to humans by female biting mosquitoes of the genus . Chloroquine and its derivates have caused the parasite to develop drug resistance in endemic areas. For this reason, new anti-malarial drugs as treatments are crucial.

In Situ Nitric Oxide Gas Nanogenerator Reprograms Glioma Immunosuppressive Microenvironment.

Universal chemotherapy in glioblastoma patients causes chemoresistance and further limits immune cells by creating an immunosuppressive tumor microenvironment that are difficult to solve by single-drug therapeutic approaches. Here, this work designs hybrid drug-loaded nanoliposomes by co-loading the chemotherapeutic drug temozolomide (TMZ) and nitric oxide (NO) prodrug JS-K with sphingosine-1-phosphate molecules (S1P) on the surface. The S1P-S1P receptors axis endows nanoliposomes with rapid targeting and lysosomal escaping capability.

An ethnomedicinal study in district, west hararghe zone, region, Ethiopia.

Traditional medicinal plants used by local people of District in west Hararghe, Ethiopia, were investigated before people's medicinal knowledge vanishes. Data on medicinal plants and demography were obtained between November 2019 and October 2020 through semi-structured interviews, group discussion and direct guided onsite observation to elicit information from 376 non-traditional and 20 traditional medicine practitioners. Ethnobotanical indices including informant consensus factor (ICF), preference ranking (PR), fidelity level (FL), relative frequency of citation (RFG) and cultural importance (CI) were employed for the data analysis.

Mystery solved? Disease detectives identify the cause of a mass die-off in the sea.

A mass sea urchin die-off in the Caribbean Sea in the 1980s resulted from a single-cell protist called a scuticociliate.

Direct Organogenesis of Citrus Cultivars from Shoot Tip Nodal Segments.

Background: Citrus cultivar improvement via conventional breeding strategies is impeded by factors related to its reproductive biology. The orange is a hybrid between pomelo () and mandarin (). Among various orange cultivars, Valencia oranges have a bit of bitter tang mixed in with their sweetness, as Navel oranges are, the most widely cultivated citrus species, quite sweeter, and also don't contain any seeds.

Functional gene-guided enrichment plus in situ microsphere cultivation enables isolation of new crucial ureolytic bacteria from the rumen of cattle.

Background: Ruminants can utilize urea as a dietary nitrogen source owing to their ability to recycle urea-N back to the rumen where numerous ureolytic bacteria hydrolyze urea into ammonia, which is used by numerous bacteria as their nitrogen source. Rumen ureolytic bacteria are the key microbes making ruminants the only type of animals independent of pre-formed amino acids for survival, thus having attracted much research interest. Sequencing-based studies have helped gain new insights into ruminal ureolytic bacterial diversity, but only a limited number of ureolytic bacteria have been isolated into pure cultures or studied, hindering the understanding of ureolytic bacteria with respect to their metabolism, physiology, and ecology, all of which are required to effectively improve urea-N utilization efficiency.

Magnetic nanoparticle loaded biodegradable vascular stents for magnetic resonance imaging and long-term visualization.

Benefiting from their good biosafety and bioabsorbability, polymeric biodegradable stents (BDSs) have promising application prospects in the treatment of cardiovascular diseases. However, due to the low density of the polymer itself, it is difficult to visualize with medical imaging techniques such as CT and MRI, which leads to difficulties in accurate BDS localization and subsequent non-invasive evaluation. Therefore, modification of BDSs to adapt to monitoring techniques for clinical use without affecting their biocompatibility and mechanical properties is a promising strategy to support the clinical translation of BDSs.

Shear stress and plaque microenvironment induce heterogeneity: A multiscale microenvironment evolution model.

Background: Both clinical images and in vivo observations have demonstrated the heterogeneity in atherosclerotic plaque composition. However, the quantitative mechanisms that contribute to the heterogeneity, such as the wall shear stress (WSS) and the interplays among microenvironmental factors are still unclear.

Methods: We develop a multiscale model coupling computational fluid dynamics, interactions of microenvironmental factors and evolutions of cellular behaviors to investigate the formation of plaque heterogeneity in a three-dimensional vessel segment.

Intracellular Construction of Cathepsin B-Guided Gadolinium Nanoparticles for Enhanced T -Weighted MR Tumor Imaging.

Magnetic resonance imaging (MRI) is a superior and noninvasive imaging technique with unlimited tissue penetration depth and superb spatiotemporal resolution, however, using intracellular self-assembly of Gd-containing nanoparticles to enhance the T -weighted MR contrast of cancer cells in vivo for precise tumor MRI is rarely reported. The lysosomal cysteine protease cathepsin B (CTSB) is regarded as an attractive biomarker for the early diagnosis of cancers and metastasis. Herein, taking advantage of a biocompatible condensation reaction, a "smart" Gd-based CTSB-responsive small molecular contrast agent VC-Gd-CBT is developed, which can self-assemble into large intracellular Gd-containing nanoparticles by glutathione reduction and CTSB cleavage to enhance the T -weighted MR contrast of CTSB-overexpressing MDA-MB-231 cells at 9.

How larvae and life cycles evolve.

Marine larvae have factored heavily in pursuits to understand the origin and evolution of animal life cycles. Recent comparisons of gene expression and chromatin state in different species of sea urchin and annelid show how evolutionary changes in embryonic gene regulation can lead to markedly different larval forms.

Emigration patterns of motile cryptofauna and their implications for trophic functioning in coral reefs.

Patterns of movement of marine species can reflect strategies of reproduction and dispersal, species' interactions, trophodynamics, and susceptibility to change, and thus critically inform how we manage populations and ecosystems. On coral reefs, the density and diversity of metazoan taxa are greatest in dead coral and rubble, which are suggested to fuel food webs from the bottom up. Yet, biomass and secondary productivity in rubble is predominantly available in some of the smallest individuals, limiting how accessible this energy is to higher trophic levels.

Axl alleviates DSS-induced colitis by preventing dysbiosis of gut microbiota.

Axl is a tyrosine kinase receptor, a negative regulator for innate immune responses and inflammatory bowel disease (IBD). The gut microbiota regulates intestinal immune homeostasis, but the role of Axl in the pathogenesis of IBD through the regulation of gut microbiota composition remains unresolved. In this study, mice with DSS-induced colitis showed increased Axl expression, which was almost entirely suppressed by depleting the gut microbiota with antibiotics.

HPr prevents FruR-mediated facilitation of RNA polymerase binding to the fru promoter in Vibrio cholerae.

Phosphorylation state-dependent interactions of the phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS) components with transcription factors play a key role in carbon catabolite repression (CCR) by glucose in bacteria. Glucose inhibits the PTS-dependent transport of fructose and is preferred over fructose in Vibrio cholerae, but the mechanism is unknown. We have recently shown that, contrary to Escherichia coli, the fructose-dependent transcriptional regulator FruR acts as an activator of the fru operon in V.

Association of IS with Multidrug Resistance in Isolates from Cattle ().

is, globally, an important cause of human illness with beef being a significant attributable source. In the human patient, systemic infection requires antibiotic therapy, and when strains are multidrug resistant (MDR), no effective treatment may be available. MDR in bacteria is often associated with the presence of mobile genetic elements (MGE) that mediate horizontal spread of antimicrobial resistance (AMR) genes.

Heterologous Expression of the Hot Pepper ABA 8'-Hydroxylase in for Phaseic Acid Production.

The CYP707A family genes encoding ABA 8'-hydroxylase catabolize abscisic acid (ABA), a plant stress hormone that plays an important role in stress condition, such as drought, heat, cold and salinity. Phaseic acid (PA) is a catabolic product of ABA. Recent studies have shown that PA is important for the physiological functions in plants.

Effects of and glycolic extracts on planktonic cells and biofilms of multidrug-resistant strains of and .

Empirical knowledge of natural plant extracts is increasingly proving to be a promising field. The effect of L. (CO) and (CA) glycolic extracts (GlExt) have potential that should be further developed in microbial tests.

A plant-produced SARS-CoV-2 spike protein elicits heterologous immunity in hamsters.

Molecular farming of vaccines has been heralded as a cheap, safe and scalable production platform. In reality, however, differences in the plant biosynthetic machinery, compared to mammalian cells, can complicate the production of viral glycoproteins. Remodelling the secretory pathway presents an opportunity to support key post-translational modifications, and to tailor aspects of glycosylation and glycosylation-directed folding.

Exploring genome gene content and morphological analysis to test recalcitrant nodes in the animal phylogeny.

An accurate phylogeny of animals is needed to clarify their evolution, ecology, and impact on shaping the biosphere. Although datasets of several hundred thousand amino acids are nowadays routinely used to test phylogenetic hypotheses, key deep nodes in the metazoan tree remain unresolved: the root of animals, the root of Bilateria, and the monophyly of Deuterostomia. Instead of using the standard approach of amino acid datasets, we performed analyses of newly assembled genome gene content and morphological datasets to investigate these recalcitrant nodes in the phylogeny of animals.

Satellites reveal hotspots of global river extent change.

Rivers are among the most diverse, dynamic, and productive ecosystems on Earth. River flow regimes are constantly changing, but characterizing and understanding such changes have been challenging from a long-term and global perspective. By analyzing water extent variations observed from four-decade Landsat imagery, we here provide a global attribution of the recent changes in river regime to morphological dynamics (e.

Estrogen-related receptor γ (ERRγ) is a key regulator of lysyl oxidase gene expression in mouse hepatocytes.

Lysyl oxidase (LOX), the copper-dependent extracellular enzyme, plays a critical role in the regulation of protein cross-linking in the extracellular matrix (ECM). It is also involved in liver regeneration and liver fibrosis. However, the mechanism of LOX regulation in mouse hepatocytes is still unclear.