Eupalinolide B inhibits periodontitis development by targeting ubiquitin conjugating enzyme UBE2D3.

Periodontitis is a chronic periodontal inflammatory disease caused by periodontal pathogens commonly seen in adults. Eupalinolide B (EB) is a sesquiterpenoid natural product extracted from Eupatorium lindleyanum and has been reported as a potential drug for cancers and immune disorders. Here, we explored the ameliorative effects and underlying molecular mechanism of EB on periodontitis for the first time.

Layer-by-layer coated probiotics with tannic acid-Ca and casein phosphopeptide complexes for caries prevention and enamel remineralization.

Dental caries is a common disease resulting from tooth demineralization caused by bacterial plaque. Probiotics have shown great potential against caries by regulating the balance of oral flora. However, obstacles such as poor colonization and lysozyme sensitivity in oral cavity hinder their further application.

Efficacy of Toothpaste Containing Polylysine and Funme Peptide on Oral Microbiome and Oral Health.

Objective: To evaluate the effect of the toothpaste containing ε-poly-L-lysine (ε-PL) and funme peptide (FP) as key components on oral microbial composition and oral health.

Methods: An oral microbiome study was initially carried out to analyze the variation in the oral microbiota before and after use of antimicrobial peptide (AMP) toothpaste. Subsequently, a clinical trial was independently performed to assess the efficacy of AMP toothpaste by measuring the dental plaque index (PLI), volatile sulfur compounds (VSCs) levels, modified bleeding index (mBI), and bleeding on probing rate (BOP%).

Effect of free gingival graft dimension on postsurgical shrinkage in posterior implant placement: A prospective parallel cohort study.

Statement Of Problem: The impact of free gingival graft (FGG) dimensions on the postsurgical shrinkage of keratinized tissue width (KTW) and surface area in posterior implant sites is unclear. Standardized assessments of how graft dimensions influence KTW and surface area shrinkage rates over a 6-month period after FGG are lacking.

Purpose: The purpose of this prospective parallel cohort study was to examine the impact of the graft dimensions on the postsurgical shrinkage of KTW and surface area over a 6-month follow-up period after FGG in the posterior regions.

Photothermal Coating on Zinc Alloy for Controlled Biodegradation and Improved Osseointegration.

Zinc (Zn) and its alloys are promising biomaterials for orthopedic applications due to their degradability and mechanical properties. Zn plays a crucial role in bone formation, but excessive early release may cause cytotoxicity and inhibit osseointegration. To solve this, we developed a near-infrared (NIR) light-controlled polycaprolactone/copper-sulfur (PCL/CuS) coating that slows degradation and enhances osseointegration of Zn alloys.

Electrically Active Biomaterials for Stimulation and Regeneration in Tissue Engineering.

In the human body, bioelectric cues are crucial for tissue stimulation and regeneration. Electrical stimulation (ES) significantly enhances the regeneration of nerves, bones, cardiovascular tissues, and wounds. However, the use of conventional devices with stimulating metal electrodes is invasive and requires external batteries.

3-MA attenuates collagen-induced arthritis in vivo via anti-inflammatory effect and autophagy inhibition.

Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease which afflicts about nearly 1% of global population. RA results in synovitis and cartilage/bone damage, even disability which aggravates the health burden. Many drugs are used to relieve RA, such as glucocorticoids (GCs), non-steroidal anti-inflammatory drugs (NSAIDs), and disease-modifying anti-rheumatic drugs (DMARDs) in the clinical treatment.

Analysis of Dynamic Mandibular Movement of Patients With Condylar Hyperplasia Treated With Orthognathic Surgery and Condylectomy.

Objective: To assess the dynamic mandibular movement of patients with condylar hyperplasia before and after simultaneous orthognathic surgery and condylectomy through an intraoral approach.

Methods: Two groups of patients diagnosed with unilateral condylar hyperplasia were studied: the preoperative group consisted of 23 patients and the postoperative group consisted of 13 patients who had undergone simultaneous orthognathic surgery and condylectomy through an intraoral approach with follow-up for more than 1 year. The normal reference value was obtained from 11 individuals without oral and maxillofacial diseases.

SHED-Derived Exosomes Ameliorate Sjögren's Syndrome-Induced Hyposalivation by Suppressing Th1 Cell Response via the miR-29a-3p/T-bet Axis.

: Sjögren's syndrome (SS), an autoimmune disease, was characterized by sicca syndrome and systemic manifestations, presenting significant treatment challenges. Exosomes, naturally derived nanoparticles containing bioactive molecules, have garnered interest in regenerative medicine. The present study aimed to elucidate the immunoregulatory properties and mechanism of exosomes obtained from the stem cells derived from human exfoliated deciduous teeth (SHED-exos) in SS-induced sialadenitis.

Association Between Chewing Capacity and Mortality Risk: The Role of Diet and Ageing.

Aim: Masticatory dysfunction due to tooth loss is a potentially modifiable risk for mortality, but the pathway behind that remains to be investigated. This prospective study aimed to examine the role of diet and ageing in the associations between chewing capacity and long-term mortality.

Methods: Data were obtained from participants (aged ≥ 20) in the National Health Nutritional and Health Survey (NHANES 1999-2010, n = 22,900).

Identification of Novel and Rare GNAS Mutations in Craniofacial Fibrous Dysplasia.

Background: Fibrous dysplasia (FD), caused by activating mutations of GNAS, is a skeletal disorder with considerable clinicopathological heterogeneity. Although prevalent mutations such as R201C and R201H dominate in FD, a limited number of rare mutations, including R201S, R201G, and Q227L, have been documented. The scarcity of information concerning these uncommon mutations motivates our investigation, seeking to enhance comprehension of this less-explored subgroup within FD.

Condylar Changes in Adult Patients with Unilateral Posterior Crossbite Following Microimplant-Assisted Rapid Palatal Expansion: A Retrospective CBCT Study.

Objectives: This study aims to assess the alterations in condylar positioning in adult skeletal Class I patients with unilateral posterior crossbite after microimplant-assisted rapid palatal expansion (MARPE) treatment.

Methods: This retrospective study involving 30 participants (10 males, 20 females) average age 22.9 ± 4.

Study on the repair function of radiation-induced salivary gland injury using human amniotic mesenchymal stem cells pre treated with hypoxia.

Objective: To investigate the reparative effect of hypoxia pretreated hAMSCs on radiation-induced damage to salivary gland function in mice.

Methods: hAMSCs were separated from human amniotic tissues by mechanical and enzymatic digestion methods and a 15 Gy electron beam was used to locally irradiate the neck of mouse to create a salivary gland injury model. The mouse models were randomly divided into four groups: control group, IR+PBS group, IR+Nor group and IR+HP group.

Optimal submicron roughness for balancing degradation behavior, immune modulation, and microbial adhesion on zinc-based barrier membranes.

Metallic zinc (Zn) has been demonstrated to be a promising alternative to barrier membrane materials for guided bone regeneration. Surface roughness significantly affects the properties of degradable Zn-based metals, especially within the Janus micro-environments of tissue regeneration. However, the effects of optimal surface roughness on Zn remain unknown.

Albumin-seeking NIR dyes for high-sensitive imaging of glomerular filtration barrier breakdown.

The kidney, vital for metabolic balance, faces risks of severe diseases if dysfunctional. The glomerular filtration barrier (GFB), crucial for blood filtration, disrupts in conditions like diabetic nephropathy or nephritides, resulting in proteinuria or even renal failure. Monitoring GFB integrity is essential for early diagnosis or prognostic monitoring.

Crosslinked Versus Non-Crosslinked Resorbable Collagen Membranes for Periodontal Regeneration: A Multicenter, Randomized, Double-Blind, Non-Inferiority Clinical Trial.

Aim: This study aimed to evaluate and compare the results of combination therapy involving bone grafting and two different resorbable collagen membranes in 1-, 2- and 3-wall infrabony defects.

Methods: A total of 174 patients with infrabony defects (≥ 7 mm periodontal probing depth) were randomized to receive deproteinized bovine bone mineral (DBBM) with either a native porcine non-crosslinked collagen membrane (N-CM, control, n = 87) or a novel porcine crosslinked collagen membrane (C-CM, test, n = 87). Clinical parameters, including periodontal probing depth (PPD), clinical attachment level (CAL), and gingival recession (GR), were recorded at baseline, 12 weeks, and 24 weeks.

Influence of buccal mucosa width/height ratio, emergence profile and buccal bone width on peri-implant tissues: a prospective one-year study.

Background: The stability of soft and hard tissues surrounding the implant is not only a matter of aesthetics, but also affects the long-term stability of the implant. The present study was to explore the influence of buccal mucosa width/height (W/H) ratio, emergence profile and buccal bone width on peri-implant soft and hard tissue changes in the posterior region.

Methods: Fifty-eight posterior implant restoration cases were recruited in this study.

Bayesian network for predicting mandibular third molar extraction difficulty.

Background: This study aimed to establish a model for predicting the difficulty of mandibular third molar extraction based on a Bayesian network to meet following requirements: (1) analyse the interaction of the primary risk factors; (2) output quantitative difficulty-evaluation results based on the patient's personal situation; and (3) identify key surgical points and propose surgical protocols to decrease complications.

Methods: Relevant articles were searched to identify risk factors. Clinical knowledge and experience were used to analyse the risk factors to establish the Bayesian network.

Effects of Long-Term Fasting on Gut Microbiota, Serum Metabolome, and Their Association in Male Adults.

Background: Long-term fasting demonstrates greater therapeutic potential and broader application prospects in extreme environments than intermittent fasting.

Method: This pilot study of 10-day complete fasting (CF), with a small sample size of 13 volunteers, aimed to investigate the time-series impacts on gut microbiome, serum metabolome, and their interrelationships with biochemical indices.

Results: The results show CF significantly affected gut microbiota diversity, composition, and interspecies interactions, characterized by an expansion of the Proteobacteria phylum (about six-fold) and a decrease in Bacteroidetes (about 50%) and Firmicutes (about 34%) populations.

Systematic identification of pathological mechanisms, prognostic biomarkers and therapeutic targets by integrating lncRNA expression variation in salivary gland mucoepidermoid carcinoma.

Biological processes intricately intertwine with tumorigenesis, significantly influencing treatment outcomes and prognosis. However, the mechanisms fostering mucoepidermoid carcinoma (MEC) remain inadequately elucidated. This research utilizes expression profiles of lncRNAs from clinical MEC tissues and matched normal glandular tissues, integrating public data to explore the biological mechanisms and immune microenvironment characteristics of tumorigenesis.

A Pyrroloquinazoline Analogue Regulated Streptococcus mutans and Streptococcus sanguinis Dual-Species Biofilms.

Objective: Selective inhibition of cariogenic bacteria is regarded as a potential strategy against caries. To assess the potential of SCH-79797, one novel promising antibiotic, in microbial equilibrium using a dual-species biofilms model of Streptococcus mutans (S. mutans) and Streptococcus sanguinis (S.

A photo-thermal dual crosslinked chitosan-based hydrogel membrane for guided bone regeneration.

Alveolar bone defects caused by inflammation or trauma jeopardize patients' oral functions. Guided bone regeneration (GBR) is widely used in repairing periodontal tissue, with barrier membranes play a crucial role in preserving the bone regeneration space. In this study, an injectable dual-crosslinked hydrogel was developed to improve the existing barrier membranes in flexibility and functionality.

Diagnostic and Therapeutic Approach to Rare Tongue Lipoma.

Lipomas are benign soft tissue tumors composed of mature adipocytes, commonly found in subcutaneous tissues. Despite their prevalence in various body regions, they are relatively rare in the oral and maxillofacial regions. This study retrospectively analyzed the clinical and imaging characteristics, as well as the treatment outcomes of 57 patients diagnosed with lipoma.