A Pilot Case-Control Study of Muscle and Bone Health Status in Patients Who Have Recovered From COVID-19 Infection.

Background: Coronavirus disease 2019 (COVID-19), resulting from the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), affects various bodily systems, including the heart, central nervous system, muscles, and bones, all of which harbor angiotensin-converting enzyme 2 (ACE-2) receptors similar to those in the respiratory system. However, research on the inflammatory response and its impact on systems such as the musculoskeletal one is relatively scarce. Our study aimed to investigate bone and muscle metrics as well as handgrip strength in individuals who recuperated from COVID-19 infection.

The neuroprotective role of volatile oils: insights into chemical profiles, characteristics, neurochemical mechanisms, and preclinical studies in Alzheimer's disease.

Volatile oils (VOs), synonymously termed essential oils (EOs), are highly hydrophobic liquids obtained from aromatic plants, containing diverse organic compounds for example terpenes and terpenoids. These oils exhibit significant neuroprotective properties, containing antioxidant, anti-inflammatory, anti-apoptotic, glutamate activation, cholinesterase inhibitory action, and anti-protein aggregatory action, making them potential therapeutic agents in managing neurodegenerative diseases (NDs). VOs regulate glutamate activation, enhance synaptic plasticity, and inhibit oxidative stress through the stimulation of antioxidant enzymes.

Assessment of heat-killed expressing Chikungunya virus E2 protein as a candidate vaccine for dual protection against Chikungunya virus and .

The Chikungunya virus (CHIKV) is a mosquito-borne virus with a long history of recurring epidemics transmitted through mosquitoes. The rapid spread of CHIKV has intensified the need for potent vaccines. Escherichia coli (), a vital part of human gut microbiota, is utilized in recombinant DNA technology for cloning.

Dengue dynamics, predictions, and future increase under changing monsoon climate in India.

The global burden of dengue disease is escalating under the influence of climate change, with India contributing a third of the total. The non-linearity and regional heterogeneity inherent in the climate-dengue relationship and the lack of consistent data makes it difficult to make useful predictions for effective disease prevention. The current study investigates these non-linear climate-dengue links in Pune, a dengue hotspot region in India with a monsoonal climate and presents a model framework for predicting both the near-term and future dengue mortalities.

Cell-free biocatalysis for co-production of nicotinamide mononucleotide and ethanol from Saccharomyces cerevisiae and recombinant Escherichia coli.

Cell-free enzyme systems have emerged as a promising approach for producing various biometabolites, offering several advantages over traditional whole-cell systems. This study presents an approach to producing nicotinamide mononucleotide (NMN) by combining a Saccharomyces cerevisiae cell-free enzyme with a recombinant Escherichia coli cell-free enzyme. The system leverages the ATP generated by yeast during ethanol fermentation to produce NMN in the presence of nicotinamide (NAM) as a substrate.

Microwave assisted synthesis of TiC-MXene for supercapacitor application.

Two-dimensional TiC MXene has been successfully synthesized using an ultrafast microwave-assisted method. Material characterization studies have confirmed the formation of a layered MXene structure. Additionally, the electrochemical performance observed for the synthesized material indicates its promising potential for use in supercapacitor applications.

Dietary supplementation and the role of phytochemicals against the Alzheimer's disease: Focus on polyphenolic compounds.

Alzheimer's disease is a complicated, multifaceted, neurodegenerative illness that places an increasing strain on healthcare systems. Due to increasing malfunction and death of nerve cells, the person suffering from Alzheimer's disease (AD) slowly and steadily loses their memories, cognitive functions and even their personality. Although medications may temporarily enhance memory, there are currently no permanent therapies that can halt or cure this irreversible neurodegenerative process.

Ritonavir enhances the efficacy of amprenavir: A promising combination therapy by targeting Leishmania DNA topoisomerase I for treatment of visceral leishmaniasis.

Visceral leishmaniasis (VL) is an opportunistic infection in HIV patients with higher relapse and mortality rate. The number of HIV-VL patients is comparatively higher in areas where both infections are endemic. However, the conventional chemotherapeutic agents have limited success due to drug toxicity, efficacy variance and overall cost of treatment.

Laccase Mediated One-Pot, Green Synthesis of Thiazoles, β-Keto Sulfones and Imidazopyridines.

We report the first in situ reaction of the β-haloketones obtained from laccase catalysed oxidation of secondary alcohol 2-halo phenylethanol's in present study. To the best of our knowledge, this is the first ever fusion of laccase catalysed oxidation reaction with green organic synthetic reaction. The methodology employs molecular oxygen to oxidize secondary alcohol in biphasic medium by laccase from T.

Integrating machine learning and structural dynamics to explore B-cell lymphoma-2 inhibitors for chronic lymphocytic leukemia therapy.

Chronic lymphocytic leukemia (CLL) is a malignancy caused by the overexpression of the anti-apoptotic protein B-cell lymphoma-2 (BCL-2), making it a critical therapeutic target. This study integrates computational screening, molecular docking, and molecular dynamics to identify and validate novel BCL-2 inhibitors from the ChEMBL database. Starting with 836 BCL-2 inhibitors, we performed ADME and Lipinski's Rule of Five (RO5) filtering, clustering, maximum common substructure (MCS) analysis, and machine learning models (Random Forest, SVM, and ANN), yielding a refined set of 124 compounds.

Comparative analysis of medical device adverse event reporting forms for patients and industries across various countries, and the introduction of a unified generic form for harmonization.

Introduction: This comparative study examines the complexities of adverse event reporting forms for medical devices across various nations. Despite rigorous clinical trials and surveillance techniques, variations persist in processes and data elements used to report adverse events. The study highlights the importance of standardizing adverse event reporting for medical devices and suggests a comprehensive 'Generic Adverse Event Reporting Form' to accurately determine causality.

Development, Pre-Clinical Safety, and Immune Profile of RENOVAC-A Dimer RBD-Based Anti-Coronavirus Subunit Vaccine.

The development of effective and safe vaccines and their timely delivery to the public play a crucial role in preventing and managing infectious diseases. Many vaccines have been produced and distributed globally to prevent COVID-19 infection. However, establishing effective vaccine development platforms and evaluating their safety and immunogenicity remains critical to increasing health security, especially in developing countries.

Exploring pyrazolines as potential inhibitors of NSP3-macrodomain of SARS-CoV-2: synthesis and in silico analysis.

COVID-19 has proved to be a global health crisis during the pandemic, and the emerging JN.1 variant is a potential threat. Therefore, finding alternative antivirals is of utmost priority.

Synthesis and performance evaluation of ZnO/CdS photoanodes with copper sulfide (CuS) and carbon counter electrodes.

The present study demonstrates the synthesis of compact ZnO layers using CdS sensitized on ZnO as a photoanode with copper sulfide (CuS) and carbon as a counter electrode (CE). In this study, a compact ZnO layer was fabricated using the simple and low-cost successive ionic layer adsorption and reaction (SILAR) method, and CuS CE films were synthesized using the chemical bath deposition method. Various characterizations, such as X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS), confirmed the formation of ZnO and CdS sensitizations on the ZnO .

Gut-brain axis and brain health: modulating neuroinflammation, cognitive decline, and neurodegeneration.

The microbiota-gut-brain axis is a pivotal medium of crosstalk between the central nervous system (CNS) and the gastrointestinal tract. It is an intricate network of synergistic molecular pathways that exert their effects far beyond their local vicinity and even affect the systemic functioning of the body. The current review explores the involvement of the gut-brain axis (GBA) in the functioning of the nervous system, with a special emphasis on the neurodegeneration, cognitive decline, and neuroinflammation that occur in Alzheimer's disease (AD) and Parkinson's disease (PD).

Role of pH in Modulating RNA-Protein Interactions in TRBP2-dsRBD2: An Interplay between Conformational Dynamics and Electrostatic Interactions.

Understanding RNA-protein interactions is crucial for uncovering the mechanisms of cellular processes and can provide insights into the basis of various diseases, paving the way for the development of targeted therapeutic interventions. Exposure to stress conditions, such as hypoxia, leads to a drop in intracellular pH, which, in turn, alters the ionization states of amino acid residues and RNA bases, affecting the charge distribution and electrostatic interactions between RNA and proteins. In addition, pH also perturbs the structure and dynamics of proteins via the disruption of H-bonds and ionic interactions.

Inferring gene-pathway associations from consolidated transcriptome datasets: an interactive gene network explorer for .

Although an established model organism, remains comparatively inaccessible to high throughput screens, and alternative bioinformatic approaches still rely on unconnected datasets and outdated algorithms. Here, we report a new approach to consolidating RNA-seq and microarray data based on a systematic exploration of parameters and computational controls, enabling us to infer functional gene associations from their co-expression patterns. To illustrate the power of this approach, we took advantage of new data regarding a previously studied pathway, the biogenesis of a secretory organelle called the mucocyst.

Exosomal signaling in cancer metastasis: Molecular insights and therapeutic opportunities.

Exosomes are membrane-bound extracellular vesicles that play a role in exchanging biological products across membranes and serve as intermediaries in intercellular communication to maintain normal homeostasis. Numerous molecules, including lipids, proteins, and nucleic acids are enclosed in exosomes. Exosomes are constantly released into the extracellular environment and exhibit distinct characteristics based on the secreted cells that produce them.

Dynamics, mechanistic and energetic evaluation of thiazole-thiadiazole compounds in flavin dependent thymidylate synthase of Mycobacterium tuberculosis.

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), remains a significant global health challenge due to the emergence of drug-resistant strains. This study targets Flavin-dependent thymidylate synthase (ThyX), an essential enzyme in the thymidylate biosynthesis pathway crucial for bacterial DNA replication. We utilized advanced computational techniques, including molecular dynamics (MD) simulations and interaction energy analysis, to examine the binding interactions and stability of various thiazole-thiadiazole compounds with Mtb ThyX.

Extract from endophytic isolates stimulates seed germination of the host and protocorm development of non-host orchids.

We isolated endophytic strains from the healthy roots, stems, and leaves of to investigate their plant growth-promoting activities in vitro. Subsequently, Indole acetic acid (IAA) was quantified and the gene (responsible for IAA synthesis in fungi) was amplified and sequenced. Finally, a germination assay was performed with seeds of and a plant growth assay with protocorms of to test their plant growth-promoting activities.

Conformational changes in 6 MeV electron beam irradiated aqueous bovine serum albumin.

Understanding the folding and unfolding mechanism of the protein is not only crucial in applications like biomedical, pharmaceutical, tissue engineering but also to the food industry. In the present study, an electron beam with 6 MeV energy derived from the Microtron accelerator was utilized to irradiate the aqueous solution of bovine serum albumin (BSA) at fluences of 5 × 10 and 10 × 10 e/cm. The control and irradiated BSA solutions were analyzed using UV-visible and FTIR spectroscopy.