Sustainable production and preparative purification of thermostable alkaline α-amylase by Bacillus simplex (ON754233) employing natural deep eutectic solvent-based extractive fermentation.

Using PEG-based deep eutectic solvents (PDES), the current study proposes extractive fermentation as a sustainable process integration for the production and purification of α-amylase from Bacillus simplex (ON754233). Glucose: PEG 400 outperformed five PDES in terms of tie lie length (58) and slope value (1.23) against sodium sulphatt.

Solving polymicrobial puzzles: evolutionary dynamics and future directions.

Polymicrobial infections include various microorganisms, often necessitating different treatment methods than a monomicrobial infection. Scientists have been puzzled by the complex interactions within these communities for generations. The presence of specific microorganisms warrants a chronic infection and impacts crucial factors such as virulence and antibiotic susceptibility.

Unveiling the therapeutic potential of a mutated paraoxonase 2 in diabetic retinopathy: Defying glycation, mitigating oxidative stress, ER stress and inflammation.

Paraoxonase 2 (PON2) is an intracellular anti-oxidant protein ubiquitously expressed in all cells and reduces reactive oxygen species, endoplasmic reticulum (ER) stress, further improves mitochondrial function and thereby shows anti-apoptotic function. In diabetes and its complications this PON gets glycated and becomes in effective. The PON activity is reported to be reduced in diabetic retinopathy and we have earlier showed Carboxy methyl lysine (AGE) decreased PON2 expression and activity in Human retinal endothelial cells (HREC) .

Cysteine desulfurase (IscS)-mediated fine-tuning of bioenergetics and SUF expression prevents hypervirulence.

Iron-sulfur (Fe-S) biogenesis requires multiprotein assembly systems, SUF and ISC, in most prokaryotes. () encodes a complete SUF system, the depletion of which was bactericidal. The ISC operon is truncated to a single gene (cysteine desulfurase), whose function remains uncertain.

A comprehensive review on therapeutic potentials of photobiomodulation for neurodegenerative disorders.

A series of experimental trials over the past two centuries has put forth Photobiomodulation (PBM) as a treatment modality that utilizes colored lights for various conditions. While in its cradle, PBM was used for treating simple conditions such as burns and wounds, advancements in recent years have extended the use of PBM for treating complex neurodegenerative diseases (NDDs). PBM has exhibited the potential to curb several symptoms and signs associated with NDDs.

Manganese doped two-dimensional zinc ferrite thin films as chemiresistive trimethylamine gas sensors.

Trimethylamine (TMA) is highly toxic and can have lethal effects on living organisms. Detecting the presence of TMA in air is very important because, if the TMA level exceeds the OSHA (Occupational Safety and Health Administration) limit, it may harm the environment and endanger human life. Doping is an appropriate flexible way to change the electrical structures of metal oxide semiconductors (MOSs) and improve their ability to detect toxic gases.

and analysis proving DPP4 inhibition and diabetes-associated gene network modulation by a polyherbal formulation: .

Dipeptidyl-peptidase IV (DPP4) inhibitors are an important class of anti-diabetic drugs recognised for their systemic biological actions. Polyherbal preparations like formulations are considered to be ideal sources for discovering novel DPP4 inhibitors owing to their rich phytochemical composition. The current study reports the DPP4 inhibitory potential of a clinically established Ayurvedic anti-diabetic formulation (NK) using assay and substantiates it by identifying potential bioactives responsible for DPP4 inhibition using computational biology tools.

Interactome analysis implicates class II transactivator (CIITA) in depression and other neuroinflammatory disorders.

Purpose: Inappropriate inflammatory responses within the nervous system (neuroinflammation) have been implicated in several neurological conditions. Class II transactivator (CIITA), a principal regulator of the major histocompatibility complex II (MHCII), is known to play essential roles in inflammation. Hence, CIITA and its interactors could be potentially involved in multiple neurological disorders.

Surface exposed and charged residues drive thermostability in fungi.

Fungi, though mesophilic, include thermophilic and thermostable species, as well. The thermostability of proteins observed in these fungi is most likely to be attributed to several molecular factors, such as the presence of salt bridges and hydrogen bond interactions between side chains. These factors cannot be generalized for all fungi.

Molecular association of and vulvovaginal candidiasis: focusing on a solution.

-mediated vulvovaginal candidiasis (VVC) is a significant challenge in clinical settings, owing to the inefficacy of current antifungals in modulating virulence, development of resistance, and poor penetration into the biofilm matrix. Various predisposition factors are molecular drivers that lead to the dysbiosis of normal microflora of the vagina, upregulation of central metabolic pathways, morphogenesis, hyphal extension, adhesion, invasion, and biofilm formation leading to chronic infection and recurrence. Hence, it is crucial to understand the molecular mechanism behind the virulence pathways driven by those drivers to decode the drug targets.

Mercuric-sulphide based metallopharmaceutical formulation as an alternative therapeutic to combat viral and multidrug-resistant (MDR) bacterial infections.

According to the Global Antimicrobial Resistance and Use Surveillance System (GLASS) data, antibiotic resistance escalates more challenges in treatment against communicable diseases worldwide. Henceforth, the use of combinational antimicrobial therapy and metal-conjugated phytoconstituents composites are considered as alternatives. The present study explored the efficacy of mercuric-sulfide-based metallopharmaceutical, Sivanar Amirtham for anti-bacterial, anti-tuberculosis, anti-HIV therapeutics and toxicity profile by haemolytic assay, first of its kind.

Enhancement of biosorption capability of imidazolium-based ionic liquid-treated for the removal of malachite green from wastewater.

Due to its toxicity effect, treating toxic pollutants discharged from textile effluent is challenging for living beings. In the present study, the comparative biosorption potential of imidazolium-based ionic liquid-treated (ILPJS) and untreated (PJS) was investigated for the removal of toxic pollutant, malachite green (MG) from aqueous solution. The textural, surface morphology, and functional analysis of ILPJS and PJS were examined using BET (Brunauer-Emmett-Teller) analysis, SEM (Scanning electron microscopy) analysis, and FTIR (Fourier-transform infrared spectroscopy) analysis.

BBD optimized antioxidants of Crotalaria candicans and its nanoconjugates, exert potent in vivo anti-biofilm effects against MRSA.

Crotalaria genus is extensively dispersed in tropical and subtropical provinces, and it is found to harbor antioxidant flavonoids. Response surface methodology-based optimization was carried out for the purpose of efficient extraction involving a suitable solvent which can maximize the yield along with higher total phenolic content and total flavonoid content (TFC). Optimization conditions for extraction of C.

Enhanced Thermoelectric Performance of -Type MgZnSb/Sb Composites: The Role of ZnSb/Sb Composites.

MgSb-based Zintl compounds have garnered recent attention as promising materials for thermoelectric applications due to their low thermal conductivity and high values as n-type materials. However, the values of -type materials are lower compared to their -type counterparts. Through a straightforward process involving cold pressing and evacuating-and-encapsulating sintering, we have successfully synthesized a variety of -type MgZnSb/Sb composites by adding the ZnSb-4%Sb composite into the MgSb host material.

A critical review on sustainable hazardous waste management strategies: a step towards a circular economy.

Globally, industrialisation and urbanisation have led to the generation of hazardous waste (HW). Sustainable hazardous waste management (HWM) is the need of the hour for a safe, clean, and eco-friendly environment and public health. The prominent waste management strategies should be aligned with circular economic models considering the economy, environment, and efficiency.

Incompatibility of Pure SnO Thin Films for Room-Temperature Gas Sensing Application.

Despite the high sensitivity and selectivity, the high operating temperature required for activation energy of tin oxide (SnO) still stands as a drawback for SnO based gas sensors. In this work, the SnO thin films were deposited through spray pyrolysis and were subjected to gas sensing at 27 °C (room temperature) towards different gases. The films exhibited a consistently low response of approximately 1 when tested to various VOCs.

Targeting BRF2: insights from screening and molecular dynamic simulations.

The Transcription factor II B (TFIIB)‑related factor 2 (BRF2) containing TFIIIB complex recruits RNA polymerase III multi-subunit complex to selective gene promoters that altogether are responsible for synthesizing a variety of small non-coding RNAs, including a special type of selenocysteine tRNA (tRNASec), micro-RNA (miRNA), and other regulatory RNAs. BRF2 has been identified as a potential oncogene that promotes cancer cell survival under oxidative stress through its genetic activation. The structure of the BRF2 protein was modeled using the Robetta server, refined, and validated using the Ramachandran plot.

Identification of coastal pesticide pollutants as potent inhibitors of urease mediated calcium carbonate precipitation: a computational approach.

Microbially induced calcite precipitation (MICP) through urease enzyme has attained a lot of recognition in various fields of civil engineering and geotechnology for stabilizing the strength of soil and various concrete materials. The activity of urease has been found to be affected by various factors like temperature, substrate concentrations, pH of the medium, presence of inhibitors, etc. Through this study, the outcome of the interaction of pesticides (commonly found in Indian coastal regions) on urease, a major organism reported for MICP studies has been investigated .

"Sharing the matrix" - a cooperative strategy for survival in Salmonella enterica serovar Typhimurium.

Background: Bacteria in nature live together in communities called biofilms, where they produce a matrix that protects them from hostile environments. The components of this matrix vary among species, with Salmonella enterica serovar Typhimurium (STm- WT) primarily producing curli and cellulose, which are regulated by the master regulator csgD. Interactions between bacteria can be competitive or cooperative, with cooperation more commonly observed among the kin population.

Estimation of biogas generation rate and carbon sequestration potential from two landfill sites in southern India.

Anaerobic digestion of organic solid waste is one of the mechanisms for sustainable development since it permits both the energy-efficient disposal of solid waste and the use of biogas. As a result, this study provides an assessment of the potential energy and emissions saved by using biogas energy generated from the biodegradation of solid waste. For present study two major cities are selected in south India namely Madurai, Tamil Nādu and Hyderabad, Telangana.

Emerging evidence-based innovative approaches to control catheter-associated urinary tract infection: a review.

Healthcare settings have dramatically advanced the latest medical devices, such as urinary catheters (UC) for infection, prevention, and control (IPC). The continuous or intermittent flow of a warm and conducive (urine) medium in the medical device, the urinary catheter, promotes the formation of biofilms and encrustations, thereby leading to the incidence of CAUTI. Additionally, the absence of an innate immune host response in and around the lumen of the catheter reduces microbial phagocytosis and drug action.

Impact of Monocular and Binocular Visual Function Parameters on Vision-Related Quality of Life in Glaucoma.

Prcis: This study describes the impact of monocular (M/O) and binocular (B/O) visual function decline, primarily the contrast sensitivity (CS) and visual field (VF) defect severity and its location, on the subjective perception of quality of life (QoL) among the Indian glaucoma population with spared central acuity.

Aim: To investigate the influence of M/O and B/O visual function parameters on vision-related QoL (VRQoL) in glaucoma with spared central visual acuity.

Materials And Methods: Glaucoma subjects underwent M/O and B/O visual acuity, CS assessment, followed by the M/O perimetry from which the integrated VF was estimated (B/O summation method).