Emerging perspectives on 3D printed bioreactors for clinical translation of engineered and bioprinted tissue constructs.

3D printed/bioprinted tissue constructs are utilized for the regeneration of damaged tissues and as models. Most of the fabricated 3D constructs fail to undergo functional maturation in conventional settings. There is a challenge to provide a suitable niche for the fabricated tissue constructs to undergo functional maturation.

Cardiac damage following renal ischemia reperfusion injury increased with excessive consumption of high fat diet but enhanced the cardiac resistance to reperfusion stress in rat.

Renal ischemia-reperfusion (IR) injury inflicts remote cardiac dysfunction. Studies on rats fed with a high-fat diet (HD) showed contradictory results: some demonstrated increased sensitivity of the heart and kidney to IR injury, while others reported resistance. In this study, we examined cardiac dysfunction and compromised cardiac tolerance associated with renal IR in HD and standard diet (SD) fed rats.

Molecular docking analysis of quercetin with known CoVid-19 targets.

Combat and care during CoVid-19 was non-trivial. Therefore, it is of interest to use the pharmacologically active plant component quercetin for the treatment of CoVid-19. Quercetin exhibits favourable ADMET values and abides by Lipinski's rule of five.

A combined crystallographic and theoretical investigation of noncovalent interactions in 1,3,4-oxadiazole-2-thione--Mannich derivatives: bioactivity and molecular docking.

Two 1,3,4-oxadiazole-2-thione--Mannich derivatives, specifically 5-(4-chlorophenyl)-3-[(2-trifluoromethylphenylamino)methyl]-1,3,4-oxadiazole-2(3)-thione (1) and 5-(4-chlorophenyl)-3-[(2,5-difluorophenylamino)methyl]-1,3,4-oxadiazole-2(3)-thione (2), were synthesized and then characterized by elemental analysis and NMR (H and C) spectroscopy and the single crystal X-ray diffraction method. The formed weak intermolecular interactions in the solid-state structures of these derivatives were thoroughly investigated utilizing a variety of theoretical tools such as Hirshfeld surface analysis and quantum theory of atoms in molecules (QTAIM). Furthermore, the CLP-PIXEL and density functional theory calculations were used to study the energetics of molecular dimers.

PM Exposure-Linked Mitochondrial Dysfunction Negates SB216763-Mediated Cardio-Protection against Myocardial Ischemia-Reperfusion Injury.

GSK3β is a promising target for treating various disease conditions, including myocardial ischemia-reperfusion injury (IR). This study investigated the potential of GSK3β as a novel drug for managing IR in rats exposed to PM for 1 day and up to 21 days. Female Wistar rats were exposed to PM at a concentration of 250 µg/m for 3 h daily for either a single day or 21 days.

Phase transitions in an adaptive network with the global order parameter adaptation.

We consider an adaptive network of Kuramoto oscillators with purely dyadic coupling, where the adaption is proportional to the degree of the global order parameter. We find only the continuous transition to synchronization via the pitchfork bifurcation, an abrupt synchronization (desynchronization) transition via the pitchfork (saddle-node) bifurcation resulting in the bistable region R_{1}. This is a smooth continuous transition to a weakly synchronized state via the pitchfork bifurcation followed by a subsequent abrupt transition to a strongly synchronized state via a second saddle-node bifurcation along with an abrupt desynchronization transition via the first saddle-node bifurcation resulting in the bistable region R_{2} between the weak and strong synchronization.

Clinical validation of electrochemical biosensor for the detection of methylglyoxal in subjects with type-2 diabetes mellitus.

Methylglyoxal (MG), a highly reactive by-product of glycolysis, is involved in the formation of advanced glycation end-products (AGEs). Elevated levels of MG have been correlated with micro-and macro-angiopathic complications in diabetes, including neuropathy, kidney disease, retinopathy, and cardiovascular disease. Therefore, point-of-care devices for detecting MG may be of great use in the screening of diabetes complications.

Evaluation of Antibiofilm and Antiquorum Sensing Activities of Fucoidan Characterized from Padina boryana against Nosocomial Pathogens.

Modern functional chemicals that can be employed in biotechnology, pharmaceutics, and food science are a sustainable source to be found in seaweeds. The bioactivity of the majority of these marine compounds has received scant research. Fucoidan is a highly sulfated polysaccharide with a range of bioactivities, including an antipathogenic effect.

Embedded 3D bioprinting - An emerging strategy to fabricate biomimetic & large vascularized tissue constructs.

Three-dimensional bioprinting is an advanced tissue fabrication technique that allows printing complex structures with precise positioning of multiple cell types layer-by-layer. Compared to other bioprinting methods, extrusion bioprinting has several advantages to print large-sized tissue constructs and complex organ models due to large build volume. Extrusion bioprinting using sacrificial, support and embedded strategies have been successfully employed to facilitate printing of complex and hollow structures.

DNA hypomethylation by fisetin preserves mitochondria functional genes and contributes to the protection of I/R rat heart.

Myocardial I/R can alter the expression of different sets of cardiac genes that negatively influence the I/R outcome via epigenetic modifications. Fisetin is known to be cardioprotective against I/R, but its underlying epigenetic mode of action is not known and is addressed in the present study. Male Wistar rats were subjected to I/R by using the Langendorff perfusion system.

Microbial Exudates as Biostimulants: Role in Plant Growth Promotion and Stress Mitigation.

Microbes hold immense potential, based on the fact that they are widely acknowledged for their role in mitigating the detrimental impacts of chemical fertilizers and pesticides, which were extensively employed during the Green Revolution era. The consequence of this extensive use has been the degradation of agricultural land, soil health and fertility deterioration, and a decline in crop quality. Despite the existence of environmentally friendly and sustainable alternatives, microbial bioinoculants encounter numerous challenges in real-world agricultural settings.

Computational analysis of crosstalk between transcriptional regulators and RNA-binding proteins suggests mutual regulation of polycomb proteins and SRSF1 influencing adult hippocampal neurogenesis.

Background: Adult hippocampal neurogenesis (AHN) is a clinically significant neural phenomenon. Understanding its molecular regulation would be important. In this regard, most studies have focused on transcriptional regulators (TRs), epigenetic modifiers, or non-coding RNAs.

An Eco-evolutionary Model on Surviving Lysogeny Through Grounding and Accumulation of Prophages.

Temperate phages integrate into the bacterial genomes propagating along with the bacterial genomes. Multiple phage elements, representing diverse prophages, are present in most bacterial genomes. The evolutionary events and the ecological dynamics underlying the accumulation of prophage elements in bacterial genomes have yet to be understood.

A New Wavelet Collocation Algorithm for Solving a Nonlinear Boundary Value Problem of the Human Corneal Shape.

The Hermite wavelet method (HWM) is introduced in this study to solve a nonlinear differential equation determining the human corneal morphology. The changes in curvature of the human cornea in hypotony, normal intraocular pressure, glaucoma, and other conditions are discussed. The Hermite wavelet operational matrices of derivatives are used to generate wavelet solutions based on this technique.

Ectopic pregnancy: search for biomarker in salivary proteome.

Ectopic pregnancy (EP) is associated with high maternal morbidity and mortality. Ultrasonography is the only dependable diagnostic tool for confirming an ectopic pregnancy. In view of inadequate early detection methods, women suffer from a high-life risk due to the severity of EP.

Novel chair graphene nanotubes as adsorbing medium for alanine and asparagine amino acids - A DFT outlook.

Amino acids are required to make protein. The deficiency of amino acids leads to a lack of sleep and mood. Among various amino acids, we conducted the adsorption studies of alanine and asparagine amino acids on a novel one-dimensional material, chair graphene nanotube.

Mercuric-sulphide based metallopharmaceutical formulation as an alternative therapeutic to combat viral and multidrug-resistant (MDR) bacterial infections.

According to the Global Antimicrobial Resistance and Use Surveillance System (GLASS) data, antibiotic resistance escalates more challenges in treatment against communicable diseases worldwide. Henceforth, the use of combinational antimicrobial therapy and metal-conjugated phytoconstituents composites are considered as alternatives. The present study explored the efficacy of mercuric-sulfide-based metallopharmaceutical, Sivanar Amirtham for anti-bacterial, anti-tuberculosis, anti-HIV therapeutics and toxicity profile by haemolytic assay, first of its kind.

Anti-quorum Sensing and Anti-biofilm Effect of Nocardiopsis synnemataformans RMN 4 (MN061002) Compound 2,6-Di-tert-butyl, 1,4-Benzoquinone Against Biofilm-Producing Bacteria.

In this study, the anti-biofilm compound of 2,6-Di-tert-butyl, 1,4-benzoquinone was purified from Nocardiopsis synnemataformans (N. synnemataformans) RMN 4 (MN061002). To confirm the compound, various spectroscopy analyses were done including ultraviolet (UV) spectrometer, Fourier transform infrared spectroscopy (FTIR), analytical high-performance liquid chromatography (HPLC), preparative HPLC, gas chromatography-mass spectroscopy (GC-MS), liquid chromatography-mass spectroscopy (LC-MS), and 2D nuclear magnetic resonance (NMR).

CESCProg: a compact prognostic model and nomogram for cervical cancer based on miRNA biomarkers.

Cervical squamous cell carcinoma, more commonly cervical cancer, is the fourth common cancer among women worldwide with substantial burden of disease, and less-invasive, reliable and effective methods for its prognosis are necessary today. Micro-RNAs are increasingly recognized as viable alternative biomarkers for direct diagnosis and prognosis of disease conditions, including various cancers. In this work, we addressed the problem of systematically developing an miRNA-based nomogram for the reliable prognosis of cervical cancer.

N-Nitrosamine sensing properties of novel penta-silicane nanosheets-a first-principles outlook.

Context: N-Nitrosamine is one of the highly toxic carcinogenic compounds that are found almost in the entire environment. In the present work, novel penta-silicene (penta-Si) and penta-silicane (penta-HSi) are utilised to sense the N-nitrosamine in the air environment. Initially, structural firmness of penta-Si and penta-HSi is confirmed using cohesive energy.