SERS as a tool for determination of structurally related compounds: The case of sulfanilamide class antibiotics.

Analysis of real objects based on surface-enhanced Raman spectroscopy (SERS) often utilizes new SERS substrates and/or complex analysis procedures, and they are optimized for only the determination of a single analyte. Moreover, analysis simplicity and selectivity are often sacrificed for maximum (sometimes unnecessary) sensitivity. Consequently, this trend limits the versatility of SERS analysis and complicates its practical implementation.

Complete genome of the Listeria monocytogenes strain AUF, used as a live listeriosis veterinary vaccine.

Listeria monocytogenes (Lm) is a highly pathogenic bacterium that can cause listeriosis, a relatively rare food-borne infectious disease that affects farm, domestic, wild animals and humans as well. The infected livestock is the frequent sources of Lm. Vaccination is one of the methods of controlling listeriosis in target farm animals to prevent Lm-associated food contamination.

Classification of musical intervals by spiking neural networks: Perfect student in solfége classes.

We investigate a spike activity of a network of excitable FitzHugh-Nagumo neurons, which is under constant two-frequency auditory signals. The neurons are supplemented with linear frequency filters and nonlinear input signal converters. We show that it is possible to configure the network to recognize a specific frequency ratio (musical interval) by selecting the parameters of the neurons, input filters, and coupling between neurons.

Multiple dyes applications for fluorescent convertible polymer capsules as macrophages tracking labels.

Tracing individual cell pathways among the whole population is crucial for understanding their behavior, cell communication, migration dynamics, and fate. Optical labeling is one approach for tracing individual cells, but it typically requires genetic modification to induce the generation of photoconvertible proteins. Nevertheless, this approach has limitations and is not applicable to certain cell types.

Fossil marine vertebrates (Chondrichthyes, Actinopterygii, Reptilia) from the Upper Cretaceous of Akkermanovka (Orenburg Oblast, Southern Urals, Russia).

Upper Cretaceous coastal marine deposits are widespread in the Southern Urals with a number of marine vertebrates previously reported from this region. However, previous studies on the vertebrate faunas in this region often lack detailed taxonomic descriptions and illustrations, rendering comparisons to other faunal assemblages difficult. A new diverse vertebrate assemblage comprising cartilaginous and bony fishes, as well as marine reptiles, is described here from the Orenburg region near Akkermanovka (Southern Urals, Russia).

Impact of white noise in artificial neural networks trained for classification: Performance and noise mitigation strategies.

In recent years, the hardware implementation of neural networks, leveraging physical coupling and analog neurons has substantially increased in relevance. Such nonlinear and complex physical networks provide significant advantages in speed and energy efficiency, but are potentially susceptible to internal noise when compared to digital emulations of such networks. In this work, we consider how additive and multiplicative Gaussian white noise on the neuronal level can affect the accuracy of the network when applied for specific tasks and including a softmax function in the readout layer.

Analysis of environmental factors influencing lumpy skin disease outbreak seasonality and assessment of its spread risk in the Saratovskaya oblast of Russia.

Background And Aim: Lumpy skin disease (LSD) is a transboundary viral disease of cattle that causes serious economic losses due to a significant decrease in meat and milk productivity. This study analyzed the influence of natural and anthropogenic environmental factors on LSD spread seasonality and assessed the risk of LSD outbreaks in the Saratovskaya oblast of the Russian Federation.

Materials And Methods: Data on LSD outbreaks and environmental factors during different seasons were collected for the period 2011-2020 in the Balkan Peninsula, Middle East, and Russia.

Biodegradable calcium carbonate carriers for the topical delivery of clobetasol propionate.

Inflammatory dermatoses represent a global problem with increasing prevalence and recurrence among the world population. Topical glucocorticoids (GCs) are the most commonly used anti-inflammatory drugs in dermatology due to a wide range of their therapeutic actions, which, however, have numerous local and systemic side effects. Hence, there is a growing need to create new delivery systems for GCs, ensuring the drug localization in the pathological site, thus increasing the effectiveness of therapy and lowering the risk of side effects.

Controlling spatiotemporal dynamics of neural networks by Lévy noise.

We explore numerically how additive Lévy noise influences the spatiotemporal dynamics of a neural network of nonlocally coupled FitzHugh-Nagumo oscillators. Without noise, the network can exhibit various partial or cluster synchronization patterns, such as chimera and solitary states, which can also coexist in the network for certain values of the control parameters. Our studies show that these structures demonstrate different responses to additive Lévy noise and, thus, the dynamics of the neural network can be effectively controlled by varying the scale parameter and the stability index of Lévy noise.

Effect of photoconversion conditions on the spectral and cytotoxic properties of photoconvertible fluorescent polymer markers.

Fluorescence labeling of cells is a versatile tool used to study cell behavior, which is of significant importance in biomedical sciences. Fluorescent photoconvertible markers based on polymer microcapsules have been recently considered as efficient and perspective ones for long-term tracking of individual cells. However, the dependence of photoconversion conditions on the polymeric capsule structure is still not sufficiently clear.

The use of machine learning for the determination of a type/model of firearms by the characteristics on cartridge cases.

Cartridge cases are commonly collected at crime scenes involving firearms. One of the stages in forensic examination is the determination of the type and model of firearms based on the class characteristics of these cartridge cases. A firearm examiner evaluates the class characteristics on the basis of their knowledge and experience, and by referring to collections of cartridge cases representing class characteristics of different firearms, special databases and reference books.

Complete electromagnetic consideration of plasmon mode excitation in graphene rectangles by incident terahertz wave.

The excitation of terahertz plasmon modes in a graphene rectangle by normally incident linearly polarized electromagnetic wave has been theoretically studied. The complete electromagnetic approach based on formulation of the integral equations for sought-for electromagnetic quantities has been developed. The influence of edge-field effects on excitation of plasmon modes for different polarization of the incident wave and different shapes of graphene rectangle has been studied.

Complete genome sequence of АМК-16, isolated from a small ruminant in the Middle Volga Region, Russia.

We report the complete genome sequence of the АМК-16, recovered from the aborted caprine fetus during a case of chlamydia infection. This 1,152,497-bp genome with 7,552-bp cryptic plasmid provides novel insights into the genetic diversity of chlamydia agent strains particularly those causing the infection in small ruminants.

Laser-Synthesized Germanium Nanoparticles as Biodegradable Material for Near-Infrared Photoacoustic Imaging and Cancer Phototherapy.

Biodegradable nanomaterials can significantly improve the safety profile of nanomedicine. Germanium nanoparticles (Ge NPs) with a safe biodegradation pathway are developed as efficient photothermal converters for biomedical applications. Ge NPs synthesized by femtosecond-laser ablation in liquids rapidly dissolve in physiological-like environment through the oxidation mechanism.

Structural studies of the O polysaccharides from the lipopolysaccharides of Azospirillum thiophilum BV-S and Azospirillum griseum L-25-5w-1.

Diazotrophic bacteria of the genus Azospirillum are known widely, because they are ubiquitous in the rhizosphere and can promote the growth and performance of nonlegume plants. Recently, more Azospirillum species have been isolated from sources other than plants or soil. We report the structures of the O polysaccharides (OPSs) from the lipopolysaccharides of the type strains A.

Combination of Machine Learning and Raman Spectroscopy for Determination of the Complex of Whey Protein Isolate with Hyaluronic Acid.

Macromolecules and their complexes remain interesting topics in various fields, such as targeted drug delivery and tissue regeneration. The complex chemical structure of such substances can be studied with a combination of Raman spectroscopy and machine learning. The complex of whey protein isolate (WPI) and hyaluronic acid (HA) is beneficial in terms of drug delivery.

Influence of optical clearing agents on the scattering properties of human nail bed and blood microrheological properties: In vivo and in vitro study.

Optical clearing agents (OCAs) are substances that temporarily modify tissue's optical properties, enabling better imaging and light penetration. This study aimed to assess the impact of OCAs on the nail bed and blood using in vivo and in vitro optical methods. In the in vivo part, OCAs were applied to the nail bed, and optical coherence tomography and optical digital capillaroscopy were used to evaluate their effects on optical clearing and capillary blood flow, respectively.

Functionalization and magnetonavigation of T-lymphocytes functionalized via nanocomposite capsules targeting with electromagnetic tweezers.

Modification of T-lymphocytes, which are capable of paracellular transmigration is a promising trend in modern personalized medicine. However, the delivery of required concentrations of functionalized T-cells to the target tissues remains a problem. We describe a novel method to functionalize T-cells with magnetic nanocapsules and target them with electromagnetic tweezers.

One-pot hydrothermal synthesis of fluorophore-modified cerium oxide nanoparticles.

Cerium oxide nanoparticles (CeO NPs), which have powerful antioxidant properties, are promising nanomaterials for the treatment of diseases associated with oxidative stress. The well-developed surface of CeO NPs makes them promising for use as a multifunctional system for various biomedical applications. This work demonstrates a simple approach that allows the direct formation of a molecular fluorophore on the surface of CeO NPs using a simple one-pot hydrothermal synthesis.

Analysis of the experimental absorption spectrum of the rabbit lung and identification of its components.

The broadband absorption coefficient spectrum of the rabbit lung presents some particular characteristics that allow the identification of the chromophores in this tissue. By performing a weighted combination of the absorption spectra of water, hemoglobin, DNA, proteins and the pigments melanin and lipofuscin, it was possible to obtain a good match to the experimental absorption spectrum of the lung. Such reconstruction provided reasonable information about the contents of the tissue components in the lung tissue, and allowed to identify a similar accumulation of melanin and lipofuscin.

Time-Delayed Anticancer Effect of an Extremely Low Frequency Alternating Magnetic Field and Multimodal Protein-Tannin-Mitoxantrone Carriers with Brillouin Microspectroscopy Visualization In Vitro.

The effect of an extremely low frequency alternating magnetic field (ELF AMF) at frequencies of 17, 48, and 95 Hz at 100 mT on free and internalized 4T1 breast cancer cell submicron magnetic mineral carriers with an anticancer drug, mitoxantrone, was shown. The alternating magnetic field (100 mT; 17, 48, 95 Hz; time of treatment-10.5 min with a 30 s delay) does not lead to the significant destruction of carrier shells and release of mitoxantrone or bovine serum albumin from them according to the data of spectrophotometry, or the heating of carriers in the process of exposure to magnetic fields.

Different Effects of Phototherapy for Rat Glioma during Sleep and Wakefulness.

There is an association between sleep quality and glioma-specific outcomes, including survival. The critical role of sleep in survival among subjects with glioma may be due to sleep-induced activation of brain drainage (BD), that is dramatically suppressed in subjects with glioma. Emerging evidence demonstrates that photobiomodulation (PBM) is an effective technology for both the stimulation of BD and as an add-on therapy for glioma.