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[An approach to complete the manual for determination of serum pirmenol levels].
Yakugaku Zasshi
November 2003
Department of Pharmacy, Department of Cardiovascular Medicine, Sapporo Medical Center NTT East, South 1, West 15, Chuo-ku, Sapporo 060-0061, Japan.
In order to complete TDM manual for pirmenol in Sapporo Medical Center NTT East, we developed HPLC method and pretreatment procedure for pirmenol samples obtained from patients. Serum (250 microliters) was alkalinized and pirmenol was extracted into n-hexane, and then the drug was again extracted into an acidic solvent, 0.044 M KH2PO4 (pH 2.
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