Development of Multiplex Assays for the Identification of Zoonotic Species.

More than one-hundred species that affect animals and humans have been described, eight of which have been associated with emerging and underdiagnosed zoonoses. Most diagnostic studies in humans have used serology or molecular assays based on the 18S rRNA gene. Because the 18S rRNA gene is highly conserved, obtaining an accurate diagnosis at the species level is difficult, particularly when the amplified DNA fragment is small.

Coumarins and Hesperetin Inhibit Human Respiratory Syncytial Virus Infection.

Respiratory syncytial virus (RSV) is one of the most prevalent viruses that causes severe acute lower respiratory tract infections (ALRTIs) in the elderly and young children. There is no specific drug to treat RSV, only a broad-spectrum antiviral, ribavirin, which is only used in critical cases. Our research group is investigating antiviral agents of natural origin, such as coumarins and flavonoids, that may help reduce or prevent RSV infection.

A Brief History of Cell Culture: From Harrison to Organs-on-a-Chip.

This comprehensive overview of the historical milestones in cell culture underscores key breakthroughs that have shaped the field over time. It begins with Wilhelm Roux's seminal experiments in the 1880s, followed by the pioneering efforts of Ross Granville Harrison, who initiated groundbreaking experiments that fundamentally shaped the landscape of cell culture in the early 20th century. Carrel's influential contributions, notably the immortalization of chicken heart cells, have marked a significant advancement in cell culture techniques.

Alternative Non-Destructive Approach for Estimating Morphometric Measurements of Chicken Eggs from Tomographic Images with Computer Vision.

The egg has natural barriers that prevent microbiological contamination and promote food safety. The use of non-destructive methods to obtain morphometric measurements of chicken eggs has the potential to replace traditional invasive techniques, offering greater efficiency and accuracy. This paper aims to demonstrate that estimates derived from non-invasive approaches, such as 3D computed tomography (CT) image analysis, can be comparable to conventional destructive methods.

Mucosal-adapted bacteriophages as a preventive strategy for a lethal Pseudomonas aeruginosa challenge in mice.

Pseudomonas aeruginosa is an emergent threat due to the antimicrobial resistance crisis. Bacteriophages (phages) are promising agents for phage therapy approaches against P. aeruginosa.

Retraction notice to "A deep learning approach based on graphs to detect plantation lines" [Heliyon Volume 10, Issue 11, 15 June 2024, e31730].

[This corrects the article DOI: 10.1016/j.heliyon.

Secondary metabolite profiling of seeds extract by GC-EI/MS and NMR analysis.

(Aristolochiaceae), commonly known as the Brazilian Dutchman's pipe, is cultivated globally as an ornamental vine, admired for the unique shape of its large flowers. In our ongoing research into the chemical composition of , the ethanol extract from its seeds was fractionated using chromatographic techniques, resulting in the isolation and identification of allantoin, along with the lignans kobusin, eudesmin, and methylarctigenin. The fractions were further analysed by NMR and GC - EI/MS, employing a molecular networking approach and retention index comparison, which led to the annotation of four sterols, eleven aliphatic alcohols, one saturated fatty acid, two unsaturated fatty acids, ten esters of saturated fatty acids, ten esters of unsaturated fatty acids, and one lactone.

Anthelmintic Potential of Conjugated Long-Chain Fatty Acids Isolated from the Bioluminescent Mushroom .

With praziquantel being the sole available drug for schistosomiasis, identifying novel anthelmintic agents is imperative. A chemical investigation of the fruiting body of the bioluminescent mushroom Berk. resulted in the isolation of new conjugated long-chain fatty acids (8,10,12,13)-12,13-dihydroxy-7-oxo-octadeca-8,10-dienoic acid () and (7,8,9,11)-7,8-dihydroxy-13-oxo-octadeca-9,11-dienoic acid () and three previously described compounds, (7,8,9)-7,8-dihydroxyoctadec-9-enoic acid (), (2)-dec-2-ene-1,10-dioic acid (), and a ketolactone marasmeno-1,15-dione ().

Color Change and Cytotoxic Effects of a Bleaching Gel With 22% Carbamide Peroxide Catalyzed by Manganese Oxide.

Objectives: To evaluate the color change and trans-amelodentinal cytotoxicity of a 22% carbamide peroxide (CP) bleaching gel containing different concentration of manganese oxide (MnO).

Material And Methods: Enamel/dentin discs adapted to artificial pulp chambers were distributed according to treatments: CN-No treatment; CP22%-22%CP; CP22 + 2MnO-22%CP + 2 mg/mLMnO; CP22% + 6MnO-22%CP + 6 mg/mLMnO; CP22% + 10MnO-22%CP + 10 mg/mLMnO applied for 2 h for 15 days. Color change-CC (ΔE and ΔWI) (n = 8) was determined at 5, 10, and 15-day periods (ANOVA/Sidak).

Asymptotic convergence for the dynamics of a Duffing-like oscillator under scaling analyses.

The dynamics of the convergence for the stationary state considering a Duffing-like equation are investigated. The driven potential for these dynamics is supplied by a damped forced oscillator that has a piecewise linear function. Fixed points and their basins of attraction were identified and measured.

Association between lifestyle risk factors and mortality in the Mexico City prospective study.

Unhealthy lifestyles risk factors, such as smoking, alcohol consumption, physical inactivity, poor diet, and obesity, have been associated with a higher risk of all-cause and cause-specific mortality. However, composite score of these unhealthy behaviours has not been considered, particularly in Latin American populations. Herein, we examined the association of lifestyle risk factors score with all-cause and cause-specific mortality in Mexican adults.

The binuclear cyclopalladated complex CP2 is targeting ubiquinol-cytochrome c reductase (complex III) of Leishmania amazonensis.

Leishmaniasis is a neglected disease that remains with a limited number of drugs available for chemotherapy and has an increased drug resistance that affects treatment outcomes. Metal-based drugs such as cyclopalladated complex [Pd(dmba)(μ-N)] (CP2), a Leishmania topoisomerase IB inhibitor involved in calcium dysregulation and mitochondrial dysfunction of the parasite, had been an alternative to outline the appearance of chemoresistance. To identify new molecular targets and point out possible resistance mechanisms, a CP2-resistant Leishmania amazonensis (LaR) was selected by stepwise exposure to increasing drug pressure until a line capable of growth in 13.

Jacalin Attenuates Colitis-Associated Colorectal Carcinogenesis by Inhibiting Tumor Cell Proliferation and Intestinal Inflammation.

Background: Colorectal cancer (CRC) remains a significant cause of morbidity and mortality worldwide. In patients with inflammatory bowel disease, who have twice the risk of developing CRC, chronic inflammation has been recognized to contribute to colitis-associated cancer (CAC) development. Jacalin, a lectin extracted from jackfruit seeds, has been shown to recognize altered glycosylation and to exert antiproliferative and cytotoxic effects in CRC.

Chronic stress triggers impairments of the redox status of salivary glands associated with different histological responses in rats.

Stress occurs as a reaction to mental and emotional pressure, anxiety, or scarring. Chronic stress is defined as constant submission to these moments. It can affect several body systems, increase blood pressure, and weaken immunity, thereby interfering with physiological health processes.

Gingivitis- and periodontitis-associated microbiota in bovine deciduous incisor teeth - A preliminary study.

As ruminants are frequently affected by periodontal diseases, understanding their microbial communities is crucial. In this pilot study, we analyzed subgingival biofilm samples of young cattle across different states: clinically healthy (n = 5), gingivitis (n = 5), and periodontitis (n = 5) using 16S rRNA gene sequencing and co-occurrence network analysis. The findings revealed that Proteobacteria was the predominant phylum across all conditions, with Fusobacteriota constituting 27.

Is self-reported symptom duration in individuals with patellofemoral pain an accurate measure? An observational longitudinal study.

Background: Although self-reported symptom duration of individuals with patellofemoral pain (PFP) is usually assessed for clinical and research purposes, its accuracy has never been investigated.

Objectives: We followed up individuals with PFP over 15 months to determine the agreement between self-reported symptom duration and calculated symptom duration.

Methods: Self-reported symptom duration of 39 participants was assessed at baseline and re-assessed at follow-up.

Comparison of principal component analysis algorithms for imputation in agrometeorological data in high dimension and reduced sample size.

Meteorological data acquired with precision, quality, and reliability are crucial in various agronomy fields, especially in studies related to reference evapotranspiration (ETo). ETo plays a fundamental role in the hydrological cycle, irrigation system planning and management, water demand modeling, water stress monitoring, water balance estimation, as well as in hydrological and environmental studies. However, temporal records often encounter issues such as missing measurements.

Unraveling the organellar genomic landscape of the therapeutic and entheogenic plant Mimosa tenuiflora: insights into genetic, structural, and evolutionary dynamics.

Mimosa tenuiflora, popularly known as "Jurema-Preta", is a perennial tree or shrub native to the tropical regions of the Americas, particularly among Afro-Brazilian and Indigenous Brazilian communities. Known for producing N,N-Dimethyltryptamine, a psychedelic compound with profound psychological effects, Jurema-Preta has been studied for its therapeutic potential in mental health. This study offers a comprehensive analysis of the plastid (ptDNA) and mitochondrion (mtDNA) genomes of M.

The translational potential of inflammation-induced skin blister human models in exploring the pathogenesis of periodontitis and its systemic health implications.

Periodontitis is a highly prevalent chronic disease. Despite decades of extensive research on the topic, a complete understanding of its immunopathogenesis, especially when linked to other inflammatory comorbidities, is lacking. human and animal experiments have shown the host inflammatory response's crucial role in both the disease's onset and its systemic implications.

A retrospective description of blood and urine alterations in 386 male cats with urethral obstruction in Botucatu, São Paulo, Brazil.

Background: Urinary retention in obstructed male cats leads to changes in blood and urine compounds, which, combined with the time of obstruction, are linked to the worsening of the animal's clinical status.

Aim: This study aimed to describe the primary laboratory changes in male cats with urethral obstruction (UO).

Methods: Medical records of 386 male cats diagnosed with UO and treated at the Veterinary Teaching Hospital of the Faculty of Veterinary Medicine and Zootechnics (FMVZ), UNESP-Botucatu, between 2010 and 2020 were reviewed.

Influence of the Chemical Structure of Perylene Derivatives on the Performance of Honey-Gated Organic Field-Effect Transistors (HGOFETs) and Their Application in UV Light Detection.

Electronics based on natural or degradable materials are a key requirement for next-generation devices, where sustainability, biodegradability, and resource efficiency are essential. In this context, optimizing the molecular chemical structure of organic semiconductor compounds (OSCs) used as active layers is crucial for enhancing the efficiency of these devices, making them competitive with conventional electronics. In this work, honey-gated organic field-effect transistors (HGOFETs) were fabricated using four different perylene derivative films as OSCs, and the impact of the chemical structure of these perylene derivatives on the performance of HGOFETs was investigated.

Potentially toxic elements (PTEs) in seabirds foraging across a heterogeneous landscape: Cross-species bioaccumulation patterns.

Seabirds are particularly susceptible to potentially toxic elements (PTEs) due to the tendency of biomagnification of some elements, thus serving as potential bioindicators for assessing environmental health. In this study, we analyzed As, Cd, Cu and Zn concentrations in liver samples from nine seabird species (51 specimens) collected along the Southwestern Atlantic Ocean. Results revealed substantial variations in PTE concentrations among species, with taxonomic orders influencing accumulation patterns.

Total coliforms, E. coli, Enterococcus spp., and Staphylococcus spp. removal in vertical tubular photobioreactor with and without support medium.

Unsafe water has severe implications for human health. Among sanitary wastewater treatment technologies, those that treat effluent in the most natural way possible (avoiding chemicals) need to be employed to minimize environmental damage upon release. Microalgae-based systems are one of the more economical and sustainable methods.

A preliminary study of cell-based bone tissue engineering into 3D-printed β-tricalcium phosphate scaffolds and polydioxanone membranes.

Treatment of complex craniofacial deformities is still a challenge for medicine and dentistry because few approach therapies are available on the market that allow rehabilitation using 3D-printed medical devices. Thus, this study aims to create a scaffold with a morphology that simulates bone tissue, able to create a favorable environment for the development and differentiation of osteogenic cells. Moreover, its association with Plenum Guide, through cell-based tissue engineering (ASCs) for guided bone regeneration in critical rat calvarial defects.