247 results match your criteria: "Sanya institute of China Agricultural University[Affiliation]"

A Susceptible Cell-Selective Delivery (SCSD) of mRNA-Encoded Cas13d Against Influenza Infection.

Adv Sci (Weinh)

January 2025

National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.

To bolster the capacity for managing potential infectious diseases in the future, it is critical to develop specific antiviral drugs that can be rapidly designed and delivered precisely. Herein, a CRISPR/Cas13d system for broad-spectrum targeting of influenza A virus (IAV) from human, avian, and swine sources is designed, incorporating Cas13d mRNA and a tandem CRISPR RNA (crRNA) specific for the highly conserved regions of viral polymerase acidic (PA), nucleoprotein (NP), and matrix (M) gene segments, respectively. Given that the virus targets cells with specific receptors but is not limited to a single organ, a Susceptible Cell Selective Delivery (SCSD) system is developed by modifying a lipid nanoparticle with a peptide mimicking the function of the hemagglutinin of influenza virus to target sialic acid receptors.

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Role of mutation G255A in modulating pyrethroid sensitivity in insect sodium channels.

Int J Biol Macromol

January 2025

School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572024, China; School of Tropical Agriculture and Forestry (School of Agricultural and Rural Affairs, School of Rural Revitalization), Hainan University, Danzhou 571700, China. Electronic address:

A voltage-gated sodium channel (VGSC) plays a crucial role in insect electrical signals, and it is a target for various naturally occurring and synthesized neurotoxins, including pyrethroids and dichlorodiphenyltrichloroethane. The type of agent is typically widely used to prevent and control sanitary and agricultural pests. The perennial use of insecticides has caused mutations in VGSCs that have given rise to resistance in most insects.

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Comparative Analysis of Myofiber Characteristics, Shear Force, and Amino Acid Contents in Slow- and Fast-Growing Broilers.

Foods

December 2024

State Key Laboratory of Animal Biotech Breeding and Frontier Science Center for Molecular Design Breeding, China Agricultural University, Beijing 100193, China.

Skeletal muscle fiber characteristics are pivotal in assessing meat quality. However, there is currently a lack of research precisely quantifying the total number of myofibers (TNM) of skeletal muscles. This study used Arbor Acres (AA) broilers and Wenchang (WC) chickens to determine the TNM of several skeletal muscles and the meat quality of the pectoralis major muscle (PM).

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Background/objectives: Climate change-induced temperature elevations pose significant challenges to livestock reproduction, particularly affecting testicular function in small ruminants. This study investigates the acute heat-stress response in goat Sertoli cells (SCs), aiming to elucidate the molecular mechanisms underlying heat-induced damage to male reproductive tissues.

Methods: SCs were isolated from testes of 4-month-old black goats and exposed to heat stress (44 °C for 2.

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The Detoxification Effects of Melatonin on Aflatoxin-Caused Toxic Effects and Underlying Molecular Mechanisms.

Antioxidants (Basel)

December 2024

National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.

Aflatoxins (AFTs) are a form of mycotoxins mainly produced by and , which are common contaminants in various agricultural sources such as feed, milk, food, and grain crops. Aflatoxin B1 (AFB1) is the most toxic one among all AFTs. AFB1 undergoes bioactivation into AFB1-8,9-epoxide, then leads to diverse harmful effects such as neurotoxicity, carcinogenicity, hepatotoxicity, reproductive toxicity, nephrotoxicity, and immunotoxicity, with specific molecular mechanisms varying in different pathologies.

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The MYB61-STRONG2 module regulates culm diameter and lodging resistance in rice.

J Integr Plant Biol

January 2025

Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.

Lodging reduces grain yield and quality in cereal crops. Lodging resistance is affected by the strength of the culm, which is influenced by the culm diameter, culm wall thickness, and cell wall composition. To explore the genetic architecture of culm diameter in rice (Oryza sativa), we conducted a genome-wide association study (GWAS).

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High temperature stress seriously affects the quality and yield of vegetable crops, especially cucumber (Cucumis sativus L.). However, the metabolic dynamics and gene regulatory network of cucumber in response to high temperature stress remain poorly studied.

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Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae) is an important pest of cowpeas, Vigna unguiculata (L.) Walp., and can cause severe damage to the crop.

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Generation of transgenic chicken through ovarian injection.

Animal Model Exp Med

December 2024

Frontiers Science Center for Molecular Design Breeding (MOE), State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural University, Beijing, China.

Background: Traditional DNA microinjection methods used in mammals are difficult to apply to avian species due to their unique reproductive characteristics. Genetic manipulation in chickens, particularly involving immature follicles within living ovaries, has not been extensively explored. This study seeks to establish an efficient method for generating transgenic chickens through ovarian injection, potentially bypassing the challenges associated with primordial germ cell (PGC) manipulation and fertilized egg microinjection.

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Background: Thrips are among the most damaging pests to cowpeas in Hainan, China. Conventional pesticide application methods often fail to achieve satisfactory control due to the small size and concealed habitats of thrips. This study aimed to enhance the efficacy of pesticides by improving their application techniques.

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Comparative efficacy of anaerobic digestion systems in removing antimicrobial resistance genes from swine wastewater.

J Hazard Mater

December 2024

Technology Innovation Center for Food Safety Surveillance and Detection (Hainan), Sanya Institute of China Agricultural University, Sanya 572025, China; National Key Laboratory of Veterinary Public Health Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address:

Swine farm wastewater is a major reservoir of antimicrobial resistance genes (ARGs). Anaerobic digestion (AD), widely implemented in farms, has been extensively studied for ARG removal. However, a comparative study on ARG removal efficiency across the four principal AD systems - up-flow anaerobic sludge blanket (UASB), continuous stirred tank reactor (CSTR), buried biogas digester (BBD), and septic tank (SPT) - is lacking.

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The Efficacy of Cecropin Against Multidrug-Resistant Bacteria Is Linked to the Destabilization of Outer Membrane Structure LPS of Gram-Negative Bacteria.

Probiotics Antimicrob Proteins

December 2024

National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.

The escalating prevalence of antibiotic-resistant bacteria has emerged as a formidable threat to global health, and the quest for alternative antimicrobial agents is imperative. Cecropins, a class of antimicrobial peptides (AMPs), have garnered attention due to their potent bactericidal properties. This investigation delves into the antibacterial prowess of Cecropin A (CA) and Cecropin AD (CAD), showcasing their robust activity against Gram-negative bacteria, inclusive of multidrug-resistant bacteria.

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Metal-Organic Framework-Based Cascade Catalysis-Enabled Fluorescent ELISA with Higher Enzymatic Stability for Zearalenone Detection in Maize.

J Agric Food Chem

January 2025

National Key Laboratory of Veterinary Public Health and Safety, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.

The enzyme-linked immunosorbent assay (ELISA) stands as one of the most frequently employed rapid detection techniques for both chemical and biological contaminants. Horseradish peroxidase (HRP) and hydrogen peroxide (HO), serving as signal generators, play indispensable roles throughout the entire ELISA process. However, HRP and HO exhibit high sensitivity to elevated temperatures, hindering the broader utilization and transportation of ELISA.

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High-performance delivery capsules co-assembled from lignin and chitosan with avermectin for sustainable pest management.

Int J Biol Macromol

December 2024

Center of Biomass Engineering/College of Agronomy and Biotechnology, China Agricultural University, Beijing, China; Sanya Institute of China Agricultural University, Sanya 572025, China. Electronic address:

Inexpensive biomass materials hold great potential for the development of green delivery systems aimed at improving the extremely low utilization efficiency of pesticides. However, current systems face challenges in achieving both high encapsulation rates and drug loading capacities. This study introduces a novel method using chitosan (CS) and sodium lignosulfonate (SL) to co-assemble with avermectin (AVM), a widely used hydrophobic pesticide, forming AVM-CS-SL micro-nano capsules.

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Characterization and Implications of the Regionally Prevalent ST8333 Strains of Serotype 4,[5],12:i: - China, 2017-2023.

China CDC Wkly

November 2024

National Health Commission Key Laboratory of Food Safety Risk Assessment, Chinese Academy of Medical Science Research Unit (No.2019RU014), China National Center for Food Safety Risk Assessment, Beijing, China.

What Is Already Known About This Topic?: is a significant foodborne pathogen responsible for millions of infections and thousands of deaths worldwide each year. The increasing prevalence of the ST34 serotype 4,5,12:i:-(. 4,[5],12:i:-) poses a substantial public health threat in China.

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Discovery of Potential Candidate Genes for Coat Colour in Wuzhishan Pigs by Integrating SNPs and mRNA Expression Analysis.

Animals (Basel)

December 2024

National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Beijing Key Laboratory for Animal Genetic Improvement, State Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding, Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

Despite identifying genes regulating the coat colour in Western pig breeds, the genetic basis of the coat colour in Chinese indigenous pigs is still not understood due to the diversity of indigenous breeds and their genetic differences from exotic pigs. In this study, 215 Wuzhishan pigs with three coat colour patterns (white, black, and black-back/white-belly) were used to conduct a genome-wide association analysis. We found that genes responsible for the coat colour in the Wuzhishan breed are located on chromosome 8.

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Salmonella enterica serovar typhimurium effectors spiA and spiC promote replication by modulating iron metabolism and oxidative stress.

Vet Microbiol

January 2025

College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China; College of Veterinary Medicine, Sanya Institute of China Agricultural University, Sanya 572000, China. Electronic address:

Salmonella enterica serovar Typhimurium (S. Typhimurium) poses a major threat to the health and safety of animal-derived foods worldwide. Recently, we have reported that S.

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Mechanisms underlying the effects of cyanogenesis on development and reproduction of Tetranychus urticae: Insights from enzyme activity and gene expression aspects.

Ecotoxicol Environ Saf

December 2024

Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; Sanya Research Academy, Chinese Academy of Tropical Agriculture Science, Sanya 572000, China. Electronic address:

Cyanogenic plants can release toxic hydrogen cyanide (HCN) to defend against herbivory by hydrolyzing the cyanogenic glycosides (CNGs) with its β-glucosidases (β-GLUs). Numerous studies have speculated this CNG-mediated toxicity by a plant-pest interaction manner. However, the specific toxic effect of HCN was not well-demonstrated because of the interference of other ingested metabolites.

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An efficient method for visualizing the plaques of Bdellovibrio bacteriovorus.

Arch Microbiol

December 2024

National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.

Bdellovibrio bacteriovorus, an obligate predator of Gram-negative bacteria, has emerged as a promising natural antibiotic to combat the escalating threat of antibiotic resistance. Plaque forming units (PFU) counting is commonly used to determine the viable numbers of B. bacteriovorus.

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Delivery and weaning are major stressful events in sows and piglets, adversely affecting production and growth performance and causing economic losses to swine farms. Probiotics as safe antibiotic alternatives have great potential for use across all stages of livestock farming. Here, 18 pregnant sows from clinical farms randomly were divided into two groups: one fed a basal diet (CON group) and the other fed a basal diet plus a probiotic mixture CBB-mix (containing 1×10 CFU/g of [CJ21], 1×10 CFU/g of [BS15], and 1×10 CFU/g of [BL21]), for 20 days before delivery.

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Combinatorial Targeting of Common Docking and ATP Binding Sites on Mps1 MAPK for Management of Pathogenic Fungi.

J Agric Food Chem

December 2024

State Key Laboratory of Maize Bio-breeding, Joint International Research Laboratory of Crop Molecular Breeding, China Agricultural University, Beijing 100193, China.

Resistance in pathogenic fungi necessitates the development of fungicides with new mechanisms of action. The Mps1 MAPK of , the pathogen of rice blast disease, has been shown to be a molecular target for fungicide research. Here, we present compound TAK-733 that interacts with the common docking (CD) site of Mps1 and can be used in combination with ATP-competitive inhibitors.

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Genetic advancements and future directions in ruminant livestock breeding: from reference genomes to multiomics innovations.

Sci China Life Sci

November 2024

Frontiers Science Center for Molecular Design Breeding (MOE); State Key Laboratory of Animal Biotech Breeding; College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.

Article Synopsis
  • * Recent advancements include better genome assemblies and the identification of genes linked to traits like meat quality, milk production, and disease resistance, enhancing our understanding of domestication and trait genetics.
  • * The emergence of new genomic tools, like graphical pangenomics, promises to revolutionize research, promoting more effective molecular breeding and accurate prediction models for improving livestock genetics.
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ZmICE1a regulates the defence-storage trade-off in maize endosperm.

Nat Plants

December 2024

State Key Laboratory of Maize Bio-breeding, Frontiers Science Center for Molecular Design Breeding, Joint International Research Laboratory of Crop Molecular Breeding, National Maize Improvement Center, College of Agronomy and Biotechnology, China Agricultural University, Beijing, China.

The endosperm of cereal grains feeds the entire world as a major food supply; however, little is known about its defence response during endosperm development. The Inducer of CBF Expression 1 (ICE1) is a well-known regulator of cold tolerance in plants. ICE1 has a monocot-specific homologue that is preferentially expressed in cereal endosperms but with an unclear regulatory function.

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Exposure to cadmium sulfate (CdSO) can lead to neurotoxicity. Nevertheless, the precise molecular mechanisms underlying this phenomenon remain unclear, and effective treatment strategies are scarce. This study explored the protective effects of ellagic acid (EA), a natural polyphenolic compound, against CdSO exposure-induced neurotoxicity in HT22 cells and the underlying molecular mechanisms.

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Publisher Correction: Tagging large CNV blocks in wheat boosts digitalization of germplasm resources by ultra-low-coverage sequencing.

Genome Biol

November 2024

Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China.

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