Pan-genome analysis and drug repurposing strategies for extensively drug-resistant Salmonella Typhi: Subtractive genomics and e-pharmacophore approaches.

In the current study, we presented the genome sequence and taxonomic classification of the new extensively drug-resistant (XDR) Salmonella enterica serovar Typhi strain JRCGR-ST-AK02. Its genome size was found to be 4,780,534 bp, containing 4864 genes. Taxonomic classification was performed based on the Average Nucleotide Identity (ANI), Genome-to-Genome Distance Calculator (GGDC) and Average Amino Acid Identity (AAI) analysis.

Drug-loaded FeO/lignin nanoparticles to treat bacterial infections.

Lignin is a biopolymer employed for various biomedical applications. Nevertheless, the drug loading and release mechanism of FeO nanoparticles with surface functionalization by lignin has yet to be described. Hence, this study functionalizes FeO nanoparticles surface with lignin (FeO/Lig) for ciprofloxacin delivery and examines its adsorption-release mechanisms.

Diagnosing gastrointestinal infections based on cycle threshold cut-offs of PCR.

This study compared the performance of molecular vs stool culture assays for gastrointestinal infection (GII) detection, with focus on defining cycle threshold (Ct) cut-off values for positive culture results. A total of 6,000 records of patients with suspected GII between October 2022 and February 2023 and registered at Clalit HealthCare Services in Haifa, Israel, were reviewed. Stool samples were collected from all patients with suspected GII.

Complete genome sequence of lytic bacteriophage BAU.Micro_SLP-22 infecting avian spp.

A lytic bacteriophage, BAU.Micro_SLP-22, was isolated from drain water in search of bio-controlling agents against avian salmonellosis. The phage genome is comprised of 59,738 bp with 56.

Rutin Synergizes with Colistin to Eradicate Salmonellosis in Mice by Enhancing the Efficacy and Reducing the Toxicity.

The wide dissemination of multidrug-resistant (MDR) Gram-negative bacteria poses a significant global health and security concern. As developing new antibiotics is generally costly, fastidious, and time-consuming, there is an urgent need for alternative therapeutic strategies to address the gap in antibiotic discovery void. This study aimed to investigate the activity of colistin (CS) in combination with a natural product, rutin (RT), to combat against Typhimurium ( Tm) in vitro and in vivo.

PHENOTYPIC RESISTANCE PROFILES OF SALMONELLA ENTERICA ISOLATED FROM WILD FELIDS IN COSTA RICA BETWEEN 2021 AND 2022.

spp. are one of the leading causes of illness, and in the last years there is an increasing interest in the role of different wild animals as reservoir of , especially multidrug resistant strains. To establish preventive and action strategies, it is essential to monitor bacterial resistance profiles and systematically collect information.

The ileocecal valve in transabdominal ultrasound. Part 2: Pathological lesions.

The aim of this paper is to present our experience in transabdominal ultrasonography of ileocecal valve lesions. The ileocecal valve, located in the central part of the ileocecal bowel segment, is rarely the primary site of disease processes. It is usually involved by pathologies in adjacent bowel segments.

Triple Co-infection With Salmonella typhi, Leptospira, and Campylobacter coli in a Patient From Guayaquil, Ecuador: Clinical and Diagnostic Challenges.

This case report describes the unusual presentation of a 32-year-old male from Guayaquil, Ecuador, who was diagnosed with a rare triple infection caused by , , and . The patient presented with persistent high fever, severe gastrointestinal symptoms, abdominal pain, and jaundice, following the consumption of street food in a resource-limited area. Important clinical findings included hepatosplenomegaly and elevated liver enzymes, which initially complicated the differential diagnosis.

Characterization and Implications of the Regionally Prevalent ST8333 Strains of Serotype 4,[5],12:i: - China, 2017-2023.

What Is Already Known About This Topic?: is a significant foodborne pathogen responsible for millions of infections and thousands of deaths worldwide each year. The increasing prevalence of the ST34 serotype 4,5,12:i:-(. 4,[5],12:i:-) poses a substantial public health threat in China.

Inactivation kinetics of and on specialty mushroom garnishes based on ramen soup broth temperature.

Recent listeriosis and salmonellosis outbreaks in the U.S. have been associated with consumption of specialty mushrooms, including enoki and wood ear.

The predominant role of FliC contributes to the flagella-related pathogenicity of ST34 S. Typhimurium monophasic variant.

Over the past two decades, the monophasic variant of Salmonella enterica serovar Typhimurium (S. Typhimurium) has rapidly emerged and increased worldwide. This upsurge is especially true for the European clone of the ST34 S.

Leveraging Lactobacillus plantarum probiotics to mitigate diarrhea and Salmonella infections in broiler chickens.

Anaerobic bacteria, such as Lactobacillus plantarum (LP), are known to play a significant role in maintaining gut health and protecting against enteric pathogens in animals. The present study aimed to develop a safe, affordable, and eco-friendly method for producing LP-based probiotics and evaluate their efficacy in mitigating Salmonella-induced diarrhea in broiler chickens. The study employed three different culture media (MRS, TSB, and Baird Parker) to grow LP, which was then dried using a spray-drying technique to produce a stable probiotic formulation.

Coinfection and Clinical Impact of Enterotoxigenic Escherichia coli Harboring Diverse Toxin Variants and Colonization Factors: 2017-2022.

Objectives: The severity of the diarrhea disease is exacerbated by co-infections that involve Enterotoxigenic Escherichia coli (ETEC) and other enteric pathogens, which complicate the diagnosis and treatment. This study explores the prevalence, clinical manifestations, and risk factors of ETEC and its co-infections in Dhaka, Bangladesh.

Methods: The study used data from the Diarrheal Disease Surveillance System at Dhaka Hospital, involving 16,276 patients from 2017-2022.

induced SIRT1 and SIRT3 are crucial for maintaining the metabolic switch in bacteria and host for successful pathogenesis.

Sirtuins are the major players in host immunometabolic regulation. However, the role of sirtuins in the modulation of the immune metabolism pertaining to salmonellosis is largely unknown. Here, our investigation focussed on the role of two important sirtuins, SIRT1 and SIRT3, shedding light on their impact on intracellular 's metabolic switch and pathogenesis establishment.

Serious infections and tuberculosis adversely impact outcomes of juvenile onset systemic lupus erythematosus in India.

Background: Infections are a major cause of morbidity and mortality in juvenile systemic lupus erythematosus (SLE). We assessed the incidence and risk factors for major infections in juvenile SLE.

Methods: A retrospective review of 225 patients of juvenile SLE (ACR 1997 criteria) with age <18 years visiting the rheumatology clinic at a single centre between 2000 to 2020 was done from case records and the hospital electronic health records.

Two multidrug-resistant clones carrying extended spectrum beta-lactamases revealed in a single hospital department by whole genome sequencing.

bacteria is a component of normal intestinal microflora of humans and animals, but can also be found in hospital settings causing urinary tract infections and sepsis. The problem of treating such infections is complicated by multidrug-resistant isolates producing extended spectrum beta-lactamases (ESBL), and the number of ESBL-carrying strains has significantly increased recently. This study presents a detailed analysis of 12 multidrug-resistant isolates obtained from the wounds of different patients in one surgical department of a multidisciplinary hospital in Moscow, Russia, using the short- and long-read whole genome sequencing.

Phenotypic Characterization and Genome Analysis of New Broad-Spectrum Virulent Salmophage, Salmonella Phage KKP_3822, for Biocontrol of Multidrug-Resistant Strains.

is one of the main foodborne pathogens. Irrational antibiotic management has led to an increase in the incidence of multidrug-resistant strains. Bacteriophages may be an alternative method of food biopreservation and contribute to reducing the number of food poisonings requiring pharmacotherapy.

and Salmonellosis in Wild Birds.

is an important bacterial pathogen in humans and warm-blooded animals. Wild bird species represent both a potential reservoir for zoonotic infection and as a susceptible host to infection by host-adapted variants. Historically, wild birds were considered to be a major source of infection in livestock, but in recent years, it has been more apparent that birds are more likely to act as a reservoir for recycling infection on farms rather than as the primary source of infection.

Dynamic Changes in the Intraepithelial Lymphocyte Numbers Following Typhimurium Infection in Broiler Chickens.

At day 21 of age, Ross-308 broilers were orally gavaged with 7.5 × 10 CFU/mL S. Typhimurium (n = 30), and another 30 birds were kept as the control.

Awareness of Poultry Farmers of Interconnected Health Risks: A Cross-Sectional Study on Mycotoxins, Biosecurity, and Salmonellosis in Jimma, Ethiopia.

Poultry farming in Ethiopia is crucial for food security and income, but it faces significant challenges due to gaps in farmer awareness. A cross-sectional study was conducted using the Biocheck.UGent™ biosecurity scoring system and a questionnaire to evaluate poultry farmers' basic and practical knowledge concerning salmonellosis and mycotoxins.

Platelet aggregation responses to Typhimurium are determined by host anti- antibody levels.

Invasive non-typhoidal infections are responsible for >75 000 deaths/year and >500 000 cases/year globally. Seventy-five percent of these cases occur in Sub-Saharan Africa, an increasing number of which are from multi-drug resistant strains. Interactions between bacteria and platelets can lead to thrombus formation, which can be beneficial for control of infection (immunothrombosis), or harmful through uncontrolled inflammation and organ damage (thromboinflammation).

THE EFFECTS OF RAIN AND DROUGHT ON INCIDENCE OF ENTERIC DISEASE IN PENNSYLVANIA (2010 - 2019).

Background: Campylobacter, nontyphoidal Salmonella, Cryptosporidium, and Giardia cause an estimated 1 million cases of domestically acquired waterborne diseases annually in the United States. Acute symptoms can include diarrhea and vomiting; however, these illnesses can result in longer term complications such as reactive arthritis, Guillan Barré syndrome and death, particularly in immunocompromised individuals. Precipitation and drought can plausibly increase the risk of enteric infections, but consensus in the literature is lacking.

Prophylactic phage administration reduces Salmonella Enteritidis infection in newly hatched chicks.

Salmonellosis outbreaks are global issues primarily associated with the consumption of poultry products, which may be infected with Salmonella. The use of lytic bacteriophages could be a safe and effective approach to reduce Salmonella prevalence in poultry and subsequently the incidence in humans. This study examined the value of prophylactic phage treatment on Salmonella levels in chickens and the effect of such treatment on their overall gut microbiome.

Biofilm Production and Antibiogram Profiles in and Salmonella.

and are important enteric pathogens associated with a variety of infections. Biofilm formation and antimicrobial resistance are important characteristics making these pathogens a concern in terms of strong attachment to substrates, expression of virulence markers and difficult removal. The present study investigates the biofilm-forming ability and antibiogram patterns among and .