Methylmalonic acidemia: brain lesions in a case of vitamin B12 non-responsive (mut0) type.

Neuropathological findings were described in a 9-day-old female infant who died of the vitamin B12 non-responsive (mut0) type of methylmalonic acidemia (MMA). Widespread karyorhectic fragments of varying size and shape were noted throughout the brain, in particular densely accumulated in the cerebellar granular layers and the layer IV of the striate cortex. Bilateral or symmetrical necrotic foci were observed in various regions of the grey matter: Sommer's sector of the hippocampus, basal ganglia, thalamus, hypothalamus and brainstem.

Development of the human gigantocellular reticular nucleus: a morphometric study.

The cytoarchitectonic development of the human gigantocellular reticular nucleus (GRN) was studied quantitatively in 15 fetuses (16-39 weeks of gestation: WG) and in 2 adults (16 and 85 years old). With microscopic observation on serial celloidin sections of the brain, we measured them to obtain the following morphometric parameters: numerical density (ND), profile area (PA), and perimeter (PL) of the GRN neurons. GRN appeared as early as early as 16-18 WG, but most neurons were still immature and the cell nucleus was relatively large (nucleocytoplasmic ratio was high), although a few large neurons containing fine Nissl bodies were observed.

[An autopsy case of brain candidiasis in premature infant: morphology and intraparenchymal distribution of Candida foci].

An autopsy case of brain candidiasis occurring in a premature infant is presented, and the morphology and intraparenchymal distribution of Candida foci are described in detail with the aid of serial sections of the affected brain. The patient was a boy, who was born after 25 weeks of gestation and died on day 15. Candida foci were composed of two infectious forms of Candida (yeasts and pseudohyphae) and various inflammatory reactions of the host.

Increased responsiveness of left ventricular apical myocardium to adrenergic stimuli.

Objective: The aim was to determine whether left ventricular apical myocardium has mechanisms to compensate for sparse sympathetic innervation.

Methods: Contractile and metabolic responses to various adrenergic stimuli and beta adrenergic receptor density were compared between left ventricular basal and apical regions in 26 anaesthetised mongrel dogs, weight 12-28 kg.

Results: Regional contractile changes in response to graded cardiac sympathetic nerve stimulation were compared among three basal (anterior, middle, and posterior) regions, and between basal middle and apical regions.

[Flow cytometric analysis of cellular DNA content in epithelial ovarian cancer].

Tumor DNA content (ploidy) was determined by flow-cytometry (FCM) on tissue from 32 epithelial ovarian cancer patients. Staining for DNA analysis was achieved with Propidium Iodide. Peripheral blood lymphocytes were used as reference diploid cell population.

[Development of human cerebellar granular layer: a morphometric study].

Development of the cerebellar granular layer, the external granular layer (EGL) and the internal granular layer (IGL), was studied morphologically to make complete serial sections of the brain from human fetuses ranging 12 to 40 weeks' gestation (WG). To examine the chronological changes and the regional differences, we measured the thickness of the layer microscopically among five different parts of the cerebellum: anterior lobe/hemisphere (AH), anterior lobe/vermis (AV), posterior lobe/hemisphere (PH), posterior lobe/vermis (PV) and flocculus (FL). EGL was the most superficial layer composed of densely packed undifferentiated cells.

Determination of effective and safe dose for intracoronary administration of nicorandil in dogs.

Study Objective: The aim was to evaluate the effective and safe dosage for intracoronary administration of nicorandil (2-nicotinamidoethyl nitrate) in dogs.

Design: Five ml of saline with or without nicorandil (0.025, 0.