Dynamics of Hydrogen Peroxide Accumulation During Tip Growth of Infection Thread in Nodules and Cell Differentiation in Pea ( L.) Symbiotic Nodules.

Hydrogen peroxide (HO) in plants is produced in relatively large amounts and plays a universal role in plant defense and physiological responses, including the regulation of growth and development. In the -legume symbiosis, hydrogen peroxide plays an important signaling role throughout the development of this interaction. In the functioning nodule, HO has been shown to be involved in bacterial differentiation into the symbiotic form and in nodule senescence.

Contrast Relative Humidity Response of Diverse Cowpea ( (L.) Walp.) Genotypes: Deep Study Using RNAseq Approach.

Cowpea ( (L.) Walp.) is appreciated for its suitability for cultivation and obtaining good yields in relatively extreme farming conditions.

Accumbal Dopamine Responses Are Distinct between Female Rats with Active and Passive Coping Strategies.

There is a gap in existing knowledge of stress-triggered neurochemical and behavioral adaptations in females. This study was designed to explore the short-term consequences of a single social defeat (SD) on accumbal dopamine (DA) dynamics and related behaviors in female Wistar rats. During the SD procedure, rats demonstrated different stress-handling strategies, which were defined as active and passive coping.

3-Diazopiperidine-2,4-diones as Convenient Precursors to Rare Polysubstituted Spiro Bis-β,γ-lactams and 2-Oxopyrrolidine-3-carboxylic Acid Derivatives via the Thermally Promoted Wolff Rearrangement.

Herein, we describe a diastereoselective and straightforward synthetic approach to polysubstituted spirocyclic bis-lactams bearing both β- and γ-lactam cores with diverse substitution patterns. The method developed is based on a microwave-assisted Wolff rearrangement/Staudinger [2 + 2] cycloaddition sequence involving 3-diazopiperidine-2,4-diones and imines. The corresponding reaction tolerates a wide range of functionalities in both substrates, giving the target bis-lactams in generally high yields.

Whole-genome sequence of strain R243 isolated from rice ( L.) roots.

We isolated an endophytic strain R243 and sequenced its genome using the NextSeq 1000 (Illumina) sequencing platform. The sequenced genome contains 4,478 protein-coding genes with a mean GC content of 45.56%.

1-(o-Tolyl)thiourea-based deep eutectic solvent as a stationary phase in flow injection analysis system for mercury and copper determination in edible oils.

In this work, a novel deep eutectic solvent (DES) composed of thymol and 1-(o Tolyl)thiourea 9/1 (mol) is presented for the first time. This DES has not been described in the literature. This DES was first used as a stationary phase in an extraction column integrated into a flow injection analysis system for the simultaneous determination of mercury and copper in edible oils.

[Case of elongated styloid process detected in forensic medical examination of skeletal remains].

There are cases of skeletal developmental features that have high identification significance for the search of missing person in forensic medical practice of skeletal remains examination. The features of the skull's anatomical structure may indicate the presence of some clinical syndromes, for which a person could have sought medical attention in the lifetime. In the described case, elongated styloid appendages of the temporal bones were identified on the skull of an unknown person found within the territory of the Leningrad region, which could be a sign of the stylohyoid syndrome (Eagle syndrome).

Automated liquid-liquid deep eutectic solvents based microextraction procedure for determination of acrylamide in foodstuffs by high-performance liquid chromatography with ultraviolet detection.

Unlabelled: For the rapid and efficient determination of acrylamide in food products by HPLC-UV, an environmentally friendly analytical approach has been developed, including liquid-phase extraction and subsequent liquid-liquid microextraction using a deep eutectic solvent. To automate the procedure, the flow-through "lab in a syringe" method was used. Acrylamide is considered a potential endocrine disrupting chemical and its main source is fried foods, which are widely consumed by both children and adults.

Ugi's amine based coordination polymers as synergistic catalysts for the electrocatalytic reduction of carbon dioxide.

The escalating concentration of carbon dioxide in the atmosphere is a pressing environmental concern, necessitating the development of efficient technologies for CO reduction and utilization. In this context, metal-organic frameworks (MOFs) emerge as promising catalysts due to their tunable structures and unique chemical properties. This study focuses on the synthesis, characterization, and evaluation of amino-functionalized MOFs with cobalt and nickel nodes for the electrochemical reduction of CO.

Inter-Strain Differences in the Lumbar Spinal Cord Anatomy and Neuromorphology: Wistar Versus Dark Agouti Rats.

Rat strains differ in physiology, behavior, and recovery after central nervous system injury. To assess these differences, we compared the gross and local anatomy and neuromorphology of the lumbar spinal cord of the Wistar and Dark Agouti (DA) strains. The key findings include (i) distinct spatial relationships between vertebrae and spinal segments in the two strains; (ii) Wistar rats have larger volumes of spinal cord gray and white matter; (iii) DA rats have smaller total neuronal populations, thus indicating an expectation of smaller local neuronal populations; (iv) this expectation was confirmed for interneurons expressing calbindin 28 kDa.

Congruence between co-occurrence and trait-based networks is scale-dependent: a case study with flea parasites of small mammalian hosts.

We applied a novel framework based on network theory and a concept of modularity that estimates congruence between trait-based ( = functional) co-occurrence networks, thus allowing the inference of co-occurrence patterns and the determination of the predominant mechanism of community assembly. The aim was to investigate the relationships between species co-occurrence and trait similarity in flea communities at various scales (compound communities: across regions within a biogeographic realm or across sampling sites within a geographic region; component communities: across sampling sites within a geographic region; and infracommunities: within a sampling site). We found that compound communities within biogeographic realms were assembled environmental or host-associated filtering.

Coupling between 2-pyridyl-selenyl chloride and phenyl-seleno-cyanate: synthesis, crystal structure and non-covalent inter-actions.

A new pyridine-fused seleno-diazo-lium salt, 3-(phenyl-selan-yl)[1,2,4]selena-diazolo[4,5-]pyridin-4-ylium chloride di-chloro-methane 0.352-solvate, CHNSe ·Cl·0.352CHCl, was obtained from the reaction between 2-pyridyl-selenenyl chloride and phenyl-seleno-cyanate.

Digital color analysis and machine learning for ballpoint pen ink clustering and aging investigation.

Fraudulent activities often involve document manipulation, which poses a significant challenge to forensic science. To address this issue, a novel method was developed that combines intended artificial UV pre-degradation, digital color analysis (DCA) of stroke images, and various machine learning (ML) models. This method can cluster blue ballpoint pen inks and predict their photodegradation time.

Description of the complete rDNA repeat unit structure of Temminck et Schlegel, 1849 (Aves).

Ribosomal RNA (18S, 5.8S, 28S) gene clusters in genomes form regions that consist of multiple tandem repeats. They are located on a single or several pairs of chromosomes and play an important role in the formation of the nucleolus responsible for the assembly of ribosome subunits.

SpLitteR: diploid genome assembly using TELL-Seq linked-reads and assembly graphs.

Background: Recent advances in long-read sequencing technologies enabled accurate and contiguous assemblies of large genomes and metagenomes. However, even long and accurate high-fidelity (HiFi) reads do not resolve repeats that are longer than the read lengths. This limitation negatively affects the contiguity of diploid genome assemblies since two haplomes share many long identical regions.

Role of Responsive Neurostimulation in Managing Drug-Resistant Epilepsy: A Systematic Review of Clinical Outcomes.

Drug-resistant epilepsy remains a substantial challenge in neurology, affecting patients who do not respond to conventional antiepileptic drugs. Responsive neurostimulation (RNS) has emerged as a promising therapeutic approach, yet comprehensive reviews synthesizing its clinical outcomes are sparse. This systematic review followed Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and involved a comprehensive database search through PubMed, Medline, Embase, the Cochrane Library, and Scopus, covering literature up to April 2024.

5-Diazo Dihydrouracils: Preparation and Some Transformations.

An approach to a new type of diazo reagents─diazo dihydrouracils─has been developed, and various transformations of the obtained diazo heterocycles have been studied, demonstrating their high synthetic potential for obtaining structurally diverse derivatives based on the privileged dihydrouracil scaffold. The X-H insertion reactions provide high yields of a variety of 5-substituted dihydrouracils. Cyclopropanation and 1,3-dipolar cycloaddition reactions involving a carbonyl ylide intermediate have been carried out to give spiro-annulated derivatives.

Handshake of deep eutectic solvent and ionic liquid: Two liquid-liquid microextraction procedures for plant analysis.

Plants are subjects of interest due to the secondary metabolites in their extracts which are promising as new pharmaceuticals. Phytochemistry do not have united system of sample preparation or analysis still due to different structure of plant cells, wide broad range of chemical properties and concentrations of bioactive compounds. Such challenges can be addressed in a green chemistry manner using new approaches through smart materials in routine monitoring and researches.

Dominant Frequency of Ventricular Fibrillation During Ischemia and Reperfusion.

Ventricular fibrillation (VF) in dogs is characterized by a rapid increase in its dominant frequency during the 1st minute of reperfusion followed by its decrease during the 2nd minute of reperfusion. The longer is ischemia in VF, the greater is the increase in dominant VF frequency during reperfusion. The 1st minute of reperfusion is characterized by a 1.

Glioblastoma Multiforme: Sensitivity to Antimicrobial Peptides LL-37 and PG-1, and Their Combination with Chemotherapy for Predicting the Overall Survival of Patients.

Glioblastomas (GBMs) are the most malignant and intractable of all cancers, with an unfavorable clinical prognosis for affected patients. The objective was to analyze the sensitivity of GBM cells to the antimicrobial peptides (AMPs) cathelicidin (LL-37) and protegrin-1 (PG-1), both alone and in combination with chemotherapy, to predict overall survival (OS) in the patients. The study was conducted on 27 GBM patients treated in the neurosurgical department of the Almazov Medical Research Centre (Saint Petersburg, Russia) from 2021 to 2024.