[Peptides prevent the forming of secretory phenotype of chondrocytes associated with the aging.].

Secretory phenotype associated with the aging (SASP) of chondrocytes forms the conditions for the musculoskeletal system diseases development, in particular, osteoarthritis (OA). The search for effective methods for OA treating is an urgent task of molecular gerontology. The purpose of this work is to characterize the SASP of chondrocytes and to conduct a comparative assessment of the effect of AED peptide and the cartilage polypeptide complex (CPC).

[Effect of renal artery stenting on the clinical and geriatric status of elderly patients with vasorenal hypertension.].

The study consists in determining the effect of renal artery stenting in patients with arterial hypertension in combination with renal artery stenosis on the dynamics of clinical and geriatric status in terms of such indicators as the number of drugs taken, cognitive status, determined by the MMSE scale, prevalence of the frequency of falls, psycho-emotional status by the Beck scale, the quality of sleep on the VAS scale, the index of age discrimination according to the E.Palmore ageism questionnaire, the quality of life according to the SF 36 questionnaire. The study involved 72 elderly patients with arterial hypertension in combination with renal artery stenosis.

[Repair stimulator alpha-glutamyl-tryptophan in the complex therapy of chronic atrophic gastritis: results of histological examination].

Objective: To study the effect of the repair stimulator alpha-glutamyl-tryptophan on the morphological characteristics of the gastric mucosa and the expression of CXCL-12 and CDX-2 in chronic atrophic gastritis associated with .

Material And Methods: Biopsy samples of 116 patients with a verified diagnosis of chronic atrophic gastritis associated with were analyzed in a multicenter double-blind randomized placebo-controlled study. During the morphological study, the parameters characterizing the process of atrophy were evaluated: the number of glands per 1 mm of the gastric mucosa, the depth of the gastric mucosa glands, the number of parietal cells per 100 epithelial cells of the gastric mucosa, and the presence of signs of intestinal metaplasia.

[Physical rehabilitation for ankle joint injuries in elderly and elderly people (Stage 2).].

The main part of the injuries of elderly and senile patients as a result of physical activity falls on the lower limbs, due to the increased load during movement. Ankle injuries have a multifactorial etiology involving the interaction of compensatory spinal and leg biomechanics, environmental factors, and shoe selection. In this regard, it is necessary to prepare a complex of rehabilitation measures, including physical therapy, with the possibility of implementing the recovery process not only on an outpatient basis, but also at home, thereby improving the quality of life and preventing disability.

[Physical rehabilitation for ankle joint injuries in elderly and elderly people (Stage 1).].

A multidisciplinary approach to recovery after injuries of the ankle joint in elderly and senile people by specialists of various profiles using drug and non-drug methods is especially relevant today. It is required to develop and put into practice various options for the combined use of physical rehabilitation means at different stages of the rehabilitation treatment of elderly and senile patients with this pathology. The study revealed no significant complications and side effects when using this technique, which allows us to recommend it as a means of secondary prevention and injuries of the ankle joint, especially with contraindications to the use of non-steroidal anti-inflammatory drugs and glucocorticosteroids.

Peptide Regulation of Chondrogenic Stem Cell Differentiation.

The search for innovative ways to treat osteoarthritis (OA) is an urgent task for molecular medicine and biogerontology. OA leads to disability in persons of middle and older age, while safe and effective methods of treating OA have not yet been discovered. The directed differentiation of mesenchymal stem cells (MSCs) into chondrocytes is considered one of the possible methods to treat OA.

Feasibility of Transport of 26 Biologically Active Ultrashort Peptides via LAT and PEPT Family Transporters.

The aim of this work is to verify the possibility of transport of 26 biologically active ultrashort peptides (USPs) into cells via LAT and PEPT family transporters. Molecular modeling and computer-assisted docking of peptide ligands revealed that the size and structure of ligand-binding sites of the amino acid transporters LAT1, LAT2, and of the peptide transporter PEPT1 are sufficient for the transport of the 26 biologically active di-, tri-, and tetra-peptides. Comparative analysis of the binding of all possible di- and tri-peptides (8400 compounds) at the binding sites of the LAT and PEPT family transporters has been carried out.

A Transgenic 5xFAD-M Line of Mice for Dendritic Spine Morphology Analysis in Alzheimer's Disease.

Cognitive impairments are closely related to synaptic loss in Alzheimer's disease (AD). Functional changes in synaptic contacts are reflected in dendritic spine morphology. Visualization of neurons for morphological studies in vivo is complicated by the fixed brain slice staining or expensive adeno-associated virus injections.

[Senescence-associated secretory phenotype and inflammaging: the role in cardiovascular diseases.].

Senescent cells take part into development the age-related diseases by formation the SASP: senescence-associated secretory phenotype. SASP is characterized by synthesis of signal molecules include proinflammatory cytokines. SASP promotes the inflammaging - chronic, low-grade, subclinical inflammatory process, that is the one of cardio-vascular diseases risk factor in older age-groups.

Senescence-Associated Secretory Phenotype of Cardiovascular System Cells and Inflammaging: Perspectives of Peptide Regulation.

A senescence-associated secretory phenotype (SASP) and a mild inflammatory response characteristic of senescent cells (inflammaging) form the conditions for the development of cardiovascular diseases: atherosclerosis, coronary heart disease, and myocardial infarction. The purpose of the review is to analyze the pool of signaling molecules that form SASP and inflammaging in cells of the cardiovascular system and to search for targets for the action of vasoprotective peptides. The SASP of cells of the cardiovascular system is characterized by a change in the synthesis of anti-proliferative proteins (p16, p19, p21, p38, p53), cytokines characteristic of inflammaging (IL-1α,β, IL-4, IL-6, IL-8, IL-18, TNFα, TGFβ1, NF-κB, MCP), matrix metalloproteinases, adhesion molecules, and sirtuins.

[Assessment of cognitive and motor functions in older age groups: clinical significance, diagnostic tools, promising directions.].

Assessment of cognitive and motor function in older age groups is carried out to identify neurological deficits, clinical and functional prognosis, determination of rehabilitation potential, organization of accessible environment, prevention of progression of geriatric syndromes. The development of an applied methodology aimed at assessing both cognitive and motor functions, as well as cognitive-motor interaction in aging in normal and pathological conditions, is at the initial stage of formation. This article presents an overview of the methods used to assess cognitive and motor functions in clinical practice, analyzes the possibilities of their use for older age groups, and considers promising areas.

[Slowdown of replicative aging of fibroblasts by hyaluronan complexes with gold nanoparticles.].

Determination the activity of the genes of sirtuin-1, hyaluronidase, TGF-β cytokine, calreticulin in the process of replicative aging of human fibroblasts in vitro and the effect of hyaluronan preparations with gold nanoparticles on the activity of replicative cell aging. Compared the expression of proteins of the studied genes using specific markers at 7 and 14 passages of cultivation of fibroblasts isolated from human skin, without drugs and in the presence of drugs in the growth medium. This work shows a decrease in the activity of the sirtuin 1 gene and an increase in the expression of hyaluronidase in the process of replicative aging of human fibroblasts.

[Modern determinants of intergenerational relationships.].

The article deals with intergenerational relationships and mutual assessments, gives an idea of the values of young people and the elderly, obtained on the basis of interviews. The authors come to the conclusion about the natural variability of intergenerational relationships, which due to adaptive capabilities, do not lead to real conflict between the studied age groups.

[Possibilities of estimation postural function in normal and pathological conditions: focus on age-related aspects.].

Age-associated disorders of the function of maintaining balance lead to an increase in the frequency of falls and related complications (injuries, limited mobility, decreased independence and autonomy, etc.). In addition, motor disorders of various genesis in most cases are accompanied by changes in postural function.

[Current problems of polypharmacy in geriatric patients when taking drugs with a risk of photosensitivity.].

The article presents an overview of the current problems of polypharmacy in geriatric patients when taking drugs with a risk of photosensitivity. The article contains information about emerging adverse drug reactions, as well as methods for diagnosing, correcting and preventing phototoxic and photoallergic reactions in patients of older age groups. The main aspects of dermatological support in the system of long-term care for geriatric patients when taking drugs with a risk of photosensitivity are outlined.

[Secretory phenotype of the human endothelial cells associated with replicative and induced aging.].

The aging-associated secretory phenotype (SASP) is a heterogeneous phenotype of cells secreting pro-inflammatory cytokines, growth factors, apoptosis' regulatory molecules, and proteases. SASP is one of the three main hallmarks of senescent cells. Dysregulation of the synthesis of SASP-forming molecules leads to the development of age-associated diseases, including cardiovascular pathology.

[Signaling molecules as biomarkers for predicting implant survival in people of different ages.].

Most patients over 50 years of age are diagnosed with diseases of the dentoalveolar apparatus, in particular, bone tissue disorders, which leads to a decrease in the survival rate of implants. Identification of the causes, as well as the development of a methodology for predicting survival by minimally invasive methods, is relevant. The aim of the study was to study the markers of tight junctions in the buccal epithelium (BE) in people of different ages.

[Transcheter aortic valve implantation as a method of treatment of chronic heart failure in elderly and old patients: Literature review (part 2).].

The most common heart valve defect among the elderly and senile is aortic valve stenosis. The traditional method of treating severe aortic valve stenosis is open surgery to replace aortic valve. At the same time, a more modern, minimally invasive method of correcting aortic stenosis is transcatheter aortic valve implantation (TAVI).

[Age-related resilience as a manifestation of neurovegetative regulation of organism's functions.].

The concept of human resilience was created to search for new approaches to improving the well-being of middle-aged, elderly and old people. Resilience is based on the physiological mechanisms of maintaining homeostasis and the ability of organs and tissues to regenerate. Identifying the correlation between environmental factors and the level of reactivity of the body is an important aspect in assessing viability.

Transport of Biologically Active Ultrashort Peptides Using POT and LAT Carriers.

Ultrashort peptides (USPs), consisting of 2-7 amino-acid residues, are a group of signaling molecules that regulate gene expression and protein synthesis under normal conditions in various diseases and ageing. USPs serve as a basis for the development of drugs with a targeted mechanism of action. The purpose of this review is to systematize the available data on USP transport involving POT and LAT transporters in various organs and tissues under normal, pathological and ageing conditions.

[Rheumatic polymyalgia is a rarely diagnosed, but often occurring disease in elderly patients.].

Verification of the diagnosis of rheumatic polymyalgia at the reception of a general practitioner, geriatrician presents significant difficulties due to the lack of pathognomonic signs of the disease. The «calling card» of the disease is a combination of severe pain in the proximal muscle groups in combination with a high acute inflammatory response.

[Features of assessing the quality of medical care for patients with chronic heart failure of older age groups (review).].

The review presents a literature analysis on the quality of medical care in general and the criteria for their assessment. The main discussion is conducted in several areas: what indicators are currently available; which of them can be used in elderly and senile age groups; what indicators should be used in this category of patients. Based on the review, it should be concluded, that the existing quality indicators do not reflect the specifics of providing care to elderly and senile people with chronic heart failure.

[Accelerated aging as a risk of the education digitalization: possibilities for prevention.].

The work is devoted to the analysis of the education digitalization negative effects and the possibilities of their prevention consideration. The urgency of this problem in the modern conditions of combating the pandemic of the new coronavirus infection and moving a significant part of education and leisure to the virtual space is noted. Against the background of a deficit in physical activity and an increase in the information consumption duration, the participants in the experiment showed a significant increase in the frequency of mental and physical maladjustment symptoms occurrence, a decrease in performance indicators and a deterioration in the biological age parameters.

Neuroepigenetic Mechanisms of Action of Ultrashort Peptides in Alzheimer's Disease.

Epigenetic regulation of gene expression is necessary for maintaining higher-order cognitive functions (learning and memory). The current understanding of the role of epigenetics in the mechanism of Alzheimer's disease (AD) is focused on DNA methylation, chromatin remodeling, histone modifications, and regulation of non-coding RNAs. The pathogenetic links of this disease are the misfolding and aggregation of tau protein and amyloid peptides, mitochondrial dysfunction, oxidative stress, impaired energy metabolism, destruction of the blood-brain barrier, and neuroinflammation, all of which lead to impaired synaptic plasticity and memory loss.

Peptides Regulating Proliferative Activity and Inflammatory Pathways in the Monocyte/Macrophage THP-1 Cell Line.

This study evaluates the effects of five different peptides, the Epitalon tetrapeptide, the Vilon dipeptide, the Thymogen dipeptide, the Thymalin peptide complex, and the Chonluten tripeptide, as regulators of inflammatory and proliferative processes in the human monocytic THP-1, which is a human leukemia monocytic cell line capable of differentiating into macrophages by PMA in vitro. These peptides (Khavinson Peptides), characterized by Prof. Khavinson from 1973 onwards, were initially isolated from animal tissues and found to be organ specific.