P-TarPmiR accurately predicts plant-specific miRNA targets.

microRNAs (miRNAs) are small non-coding ribonucleic acids that post-transcriptionally regulate gene expression through the targeting of messenger RNA (mRNAs). Most miRNA target predictors have focused on animal species and prediction performance drops substantially when applied to plant species. Several rule-based miRNA target predictors have been developed in plant species, but they often fail to discover new miRNA targets with non-canonical miRNA-mRNA binding.

Pyoluteorin and 2,4-diacetylphloroglucinol are major contributors to Pf-5 biocontrol against in cannabis.

Pf-5 is an effective biocontrol agent that protects many crops against pathogens, including the fungal pathogen causing gray mold disease in crops. Previous studies have demonstrated the important role of antibiotics pyoluteorin (PLT) and 2,4-diacetylphloroglucinol (DAPG) in Pf-5-mediated biocontrol. To assess the potential involvement of PLT and DAPG in the biocontrol exerted by Pf-5 against in the phyllosphere of , two knockout Pf-5 mutants were generated by in-frame deletion of genes or , required for the synthesis of PLT or DAPG respectively, using a two-step allelic exchange method.

Biodiversity in a Cool-Climate Vineyard: A Case Study from Quebec.

In Quebec (Canada), viticulture has experienced steady growth in the last 35 years in terms of surfaces cultivated and value, although it is practiced in climatic conditions at the edge of what is considered a cool-climate area. This case study documents biodiversity studies conducted at the l'Orpailleur vineyard (Dunham, QC, Canada) from 1997 to 2021. In a first phase starting in 1997, the biodiversity of insecticide-free and insecticide-treated plots was determined for the taxa Scarabaeidae, Curculionidae, Chrysomelidae, Cicadellidae, Acari and Aranae.

Biocontrol Activity of spp. and spp. Against and Other Cannabis Fungal Pathogens.

Gray mold caused by is one of the most widespread and damaging diseases in cannabis crops worldwide. With challenging restrictions on pesticide use and few effective control measures, biocontrol agents are needed to manage this disease. The aim of this study was to identify bacterial biocontrol agents with wide-spectrum activity against .

Phloroglucinol Derivatives in Plant-Beneficial spp.: Biosynthesis, Regulation, and Functions.

Plant-beneficial spp. aggressively colonize the rhizosphere and produce numerous secondary metabolites, such as 2,4-diacetylphloroglucinol (DAPG). DAPG is a phloroglucinol derivative that contributes to disease suppression, thanks to its broad-spectrum antimicrobial activity.

Pest Activity and Protection Practices: Four Decades of Transformation in Quebec Apple Orchards.

A group of commercial orchards from Quebec (Canada) was followed from 1977 to 2019 as part of a project to implement Integrated Pest Management (IPM) practices. Collected data comprised activity of major fruit pests (from monitoring traps), fruit damage at harvest and pesticide applications, from which the annual costs and impacts of protection programs over 42 years were calculated. Activity and fruit damage in commercial orchards were compared to patterns observed in a reference insecticide-free orchard.

Inoculation With the Plant-Growth-Promoting Rhizobacterium LBUM677 Impacts the Rhizosphere Microbiome of Three Oilseed Crops.

The bacterial communities inhabiting the rhizosphere play an important role in plant development and health. Here we studied the effect of inoculation with LBUM677, a plant growth promoting rhizobacterium that promotes seed oil accumulation, on the rhizosphere microbiome of three oilseed crops (, , and ) over time. Next-Generation high-throughput sequencing targeting the V4 region of 16S rDNA was used to characterize the microbial communities associated with the three different crops, inoculated or not with LBUM677, over a time period of up to 90 days post-inoculation.

Diversity and Virulence of spp. Causing Potato Common Scab in Prince Edward Island, Canada.

Common scab (CS) is a potato disease that significantly decreases the market value of potato tubers after the development of necrotic lesions on their surface. is the main causal agent of CS; however, other closely related species, including and , have also been shown to cause the disease. In this study, we characterized the genetic and phenotypic diversity of spp.

Transcriptome alteration in Phytophthora infestans in response to phenazine-1-carboxylic acid production by Pseudomonas fluorescens strain LBUM223.

Background: Phytophthora infestans is responsible for late blight, one of the most important potato diseases. Phenazine-1-carboxylic acid (PCA)-producing Pseudomonas fluorescens strain LBUM223 isolated in our laboratory shows biocontrol potential against various plant pathogens. To characterize the effect of LBUM223 on the transcriptome of P.