Immunohistochemistry Evaluation of the Effect in Vivo of Tumor Necrosis Factor (TNF)-alpha on Blood Vessel Density in Murine Fibrosarcoma.

Purpose: Angiogenesis is essential for tumor growth and metastases, thus bestowing obvious importance upon methodologies which could enable its inhibition.

Materials: C57BL/6 female mice bearing a subcutaneous (s.c.

Primary liposarcoma of the mediastinum.

Patient. A 62-year-old man presented with effort dyspnea, non-productive cough and weakness of 4 month duration. He had no findings on physical examination.

A memory-shape temperature-dependent nickel-titanium device for colonic anastomosis in laparoscopic surgery.

Minimally invasive surgery is a growing issue in medicine and is also increasingly being used for colonic surgery. With this procedure, the involved colon is dissected laparoscopically, exteriorized through a small incision and the segment containing the tumor is resected. The anastomosis is done extraperitoneally either by hand suture or with a stapler.

Cancer immunotherapy: potential involvement of mediators.

The description of a cell-free soluble anti-tumour factor by Carswell et al. in 1975 (Proc Natl Acad Sci USA, 72: 3666-3670) was followed by a long series of experimental and clinical investigations into the role of cell-free mediators in cancer immunotherapy. These investigations included research on the effects of macrophage-derived eicosanoids (cycloxygenase and lipoxygenase derivates of arachidonic acid) and of monokines such as tumour necrosis factor-alpha, interleukin-1 and granulocyte-monocyte-macrophage-colony stimulating factor) and of lymphocyte products: interleukins and interferons.

Anti- schistosomular activity of human monocytes/macrophages in response to interleukin-3 and granulocyte-macrophage colonystimulating factor stimulation.

Human monocytes, co-incubated for 7 days in culture with GM-CSF or IL-3 but not with IFN-gamma, exerted a variable schistosotnulicidal effect on Schistosoma mansoni parasites when grown in 96-well round-bottomed plates but not in flat-bottomed plates. Addition of LPS or IFN-gamma or both, for the last 48 h did not enhance the cidal effect. Addition of LPS but not IFN-gamma to the pre-incubated cells with GM-CSF or IL-3 markedly stimulated TNF-alpha production by the cells but not their cidal activity.