CBX2 suppresses interferon signaling to diminish tumor immunogenicity via a noncanonical corepressor complex.

Chromobox 2 (CBX2), a crucial component of the polycomb repressive complex (PRC), has been implicated in the development of various human cancers. However, its role in the regulation of tumor immunogenicity and immune evasion remains inadequately understood. In this study, we found that ablation of CBX2 led to tumor growth inhibition, activation of the tumor immune microenvironment, and enhanced therapeutic efficacy of anti-PD1 or adoptive T cell therapies by using murine syngeneic tumor models.

Application of attenuated total reflection-Fourier transform infrared spectroscopy in semi-quantification of blood lipids and characterization of the metabolic syndrome.

Background/purpose: Dyslipidemia, a hallmark of metabolic syndrome (MetS), contributes to atherosclerotic and cardiometabolic disorders. Due to days-long analysis, current clinical procedures for cardiotoxic blood lipid monitoring are unmet. This study used AI-assisted attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy to identify MetS and precisely quantify multiple blood lipid levels with a blood sample of 0.

Hypointense Findings on Hepatobiliary Phase MR Images.

Hepatobiliary (HB) contrast agents are increasingly valuable diagnostic tools in MRI, offering a wider range of applications as their clinical use expands. Normal hepatocytes take up HB contrast agents, which are subsequently excreted in bile. This property creates a distinct HB phase providing valuable insights into liver function and biliary anatomy.

ABCA4 Deep Intronic Variants Contributed to Nearly Half of Unsolved Stargardt Cases With a Milder Phenotype.

Purpose: The purpose of this study was to investigate the contribution and natural progression of ABCA4 deep intronic variants (DIVs) among a Chinese Stargardt disease (STGD) cohort.

Methods: For unsolved STGD probands, DIVs in ABCA4 were detected by next-generation sequencing, and splicing effects were evaluated by in silico tools and validated through minigene experiments. Comprehensive ocular examinations, especially fundus changes, were carried out and analyzed.

Defense guard: strategies of plants in the fight against Cadmium stress.

Soil Cadmium (Cd) contamination is a worldwide problem with negative impacts on human health. Cultivating the Cd-Pollution Safety Cultivar (Cd-PSC) with lower Cd accumulation in edible parts of plants is an environmentally friendly approach to ensure food security with wide application prospects. Specialized mechanisms have been addressed for Cd accumulation in crops.

Therapeutic gene correction of HBB frameshift CD41-42 (-TCTT) deletion in human hematopoietic stem cells.

Β-thalassemia is one of the global health burdens. The CD41-42 (-TCTT) mutation at HBB is the most prevalent pathogenic mutation of β-thalassemia in both China and Southeast Asia. Previous studies focused on repairing the HBB CD41-42 (-TCTT) mutation in β-thalassemia patient-specific induced pluripotent stem cells, which were subsequently differentiated into hematopoietic stem and progenitor cells (HSPCs) for transplantation.

Adenine base editor corrected ADPKD point mutations in hiPSCs and kidney organoids.

Autosomal dominant polycystic kidney disease (ADPKD) is a dominant genetic disorder caused primarily by mutations in the PKD1 gene, resulting in the formation of numerous cysts and eventually kidney failure. However, there are currently no gene therapy studies aimed at correcting PKD1 gene mutations. In this study, we identified two mutation sites associated with ADPKD, c.

Comparative study of Mg/Al-LDH and Mg/Fe-LDH on adsorption and loss control of 2,4-dichlorophenoxyacetic acid.

Low efficiency and high surface runoff of 2,4-dichlorophenoxyacetic acid (2,4-D) from agricultural field threaten crop yield severely. Layered double hydroxides (LDH) have shown promising adsorption properties for 2,4-D. However, the comparison of two environmentally friendly LDHs (i.

Increasing the robustness of Escherichia coli for aromatic chemicals production through transcription factor engineering.

Engineering microbial cell factories has been widely used to produce a variety of chemicals, including natural products, biofuels, and bulk chemicals. However, poor robustness limits microbial production on an industrial scale. Microbial robustness is essential to ensure reliable and sustainable production of targeted chemicals.

Targeting oncogene-induced cellular plasticity for tumor therapy.

Cellular plasticity, the remarkable adaptability of cancer cells to survive under various stress conditions, is a fundamental hallmark that significantly contributes to treatment resistance, tumor metastasis, and disease recurrence. Oncogenes, the driver genes that promote uncontrolled cell proliferation, have long been recognized as key drivers of cellular transformation and tumorigenesis. Paradoxically, accumulating evidence demonstrates that targeting certain oncogenes to inhibit tumor cell proliferation can unexpectedly induce processes like epithelial-to-mesenchymal transition (EMT), conferring enhanced invasive and metastatic capabilities.

Abscission in plants: from mechanism to applications.

Abscission refers to the natural separation of plant structures from their parent plants, regulated by external environmental signals or internal factors such as stress and aging. It is an advantageous process as it enables plants to shed unwanted organs, thereby regulating nutrient allocation and ensuring the dispersal of fruits and seeds from the parent. However, in agriculture and horticulture, abscission can severely reduce crop quality and yield.

Roots to the rescue: how plants harness hydraulic redistribution to survive drought across contrasting soil textures.

Hydraulic redistribution (HR) is a critical ecological process whereby plant roots transfer water from wetter to drier soil layers, significantly impacting soil moisture dynamics and plant water and nutrient uptake. Yet a comprehensive understanding of the mechanism triggering HR and its influencing factors remains elusive. Here, we conducted a systematic meta-analysis to discuss the influence of soil conditions and plant species characteristics on HR occurrence.

Development of a CRISPR-Cas12a based assay for the detection of swine enteric coronaviruses in pig herds in China.

Porcine epidemic diarrhea virus (PEDV), Transmissible gastroenteritis virus (TGEV), Porcine deltacoronavirus (PDCoV) and Swine acute diarrhea syndrome coronavirus (SADS-CoV) rank among the most frequently encountered swine enteric coronaviruses (SECoVs), leading to substantial economic losses to the swine industry. The availability of a rapid and highly sensitive detection method proves beneficial for the monitoring and surveillance of SECoVs. Based on the N genes of four distinct SECoVs, a novel detection method was developed in this study by combining recombinant enzyme polymerase isothermal amplification (RPA) with clustered regularly interspaced short palindromic repeats (CRISPR)-associated proteins (Cas) 12a.

Mechanisms underlying the interactions and adaptability of nitrogen removal microorganisms in freshwater sediments.

Microorganisms in eutrophic water play a vital role in nitrogen (N) removal, which contributes significantly to the nutrient cycling and sustainability of eutrophic ecosystems. However, the mechanisms underlying the interactions and adaptation strategies of the N removal microorganisms in eutrophic ecosystems remain unclear. We thus analyzed field sediments collected from a eutrophic freshwater ecosystem, enriched the N removal microorganisms, examined their function and adaptability through amplicon, metagenome and metatranscriptome sequencing.

Technologies for studying phase-separated biomolecular condensates.

Biomolecular condensates, also referred to as membrane-less organelles, function as fundamental organizational units within cells. These structures primarily form through liquid-liquid phase separation, a process in which proteins and nucleic acids segregate from the surrounding milieu to assemble into micron-scale structures. By concentrating functionally related proteins and nucleic acids, these biomolecular condensates regulate a myriad of essential cellular processes.

Metabolic engineering for single-cell protein production from renewable feedstocks and its applications.

Proteins are indispensable for maintaining a healthy diet and performing crucial functions in a multitude of physiological processes. The growth of the global population and the emergence of environmental concerns have significantly increased the demand for protein-rich foods such as meat and dairy products, exerting considerable pressure on global food supplies. Single-cell proteins (SCP) have emerged as a promising alternative source, characterized by their high protein content and essential amino acids, lipids, carbohydrates, nucleic acids, inorganic salts, vitamins, and trace elements.

Winter forage crops influence soil properties through establishing different arbuscular mycorrhizal fungi communities in paddy field.

Winter planting is promising for improving the utilization rate of fallow paddy fields in southern China by establishing arbuscular mycorrhizal fungi (AMF) communities. However, the effects of different winter forage crops on AMF community construction remain unknown. The AMF community establishment of different winter planting forage crops were conducted in oat, rye, Chinese milk vetch, and ryegrass, with winter fallow as a control.

DSCI: a database of synthetic biology components for innate immunity and cell engineering decision-making processes.

Although significant progress of clinical strategy has been made in gene editing and cell engineering in immunotherapy, it is now apparent that design and modification in terms of complex signaling pathways and motifs on medical synthetic biology are still full of challenges. Innate immunity, the first line of host defense against pathogens, is critical for anti-pathogens immune response as well as regulating durable and protective T cell-mediated anti-tumor responses. Here, we introduce DSCI (Database of Synthetic Biology Components for Innate Immunity, https://dsci.

Decoding the genetic blueprint: regulation of key agricultural traits in sorghum.

Sorghum, the fifth most important crop globally, thrives in challenging environments such as arid, saline-alkaline, and infertile regions. This remarkable crop, one of the earliest crops domesticated by humans, offers high biomass and stress-specific properties that render it suitable for a variety of uses including food, feed, bioenergy, and biomaterials. What's truly exciting is the extensive phenotypic variation in sorghum, particularly in traits related to growth, development, and stress resistance.

From trade-off to synergy: how nutrient status modulates plant resistance to herbivorous insects?

The principle of the "growth-defense trade-off" governs how plants adjust their growth and defensive strategies in response to external factors, impacting interactions among plants, herbivorous insects, and their natural enemies. Mineral nutrients are crucial in modulating plant growth and development through their bottom-up effects. Emerging evidence has revealed complex regulatory networks that link mineral nutrients to plant defense responses, influencing the delicate balance between growth and defense against herbivores.