SARS-CoV-2 vaccine based on ferritin complexes with screened immunogenic sequences from the Fv-antibody library.

In this study, the vaccine against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was developed using ferritin complexes with the immunogenic sequences screened against the SARS-CoV-2 spike protein (SP) from the Fv-antibody library. The Fv-antibody library was prepared on the outer membrane of by the expression of the V region of immunoglobulin G (IgG) with a randomized complementarity-determining region 3 (CDR3). Four Fv-antibodies to the receptor-binding domain (RBD) were screened from the Fv-antibody library, which had a comparable binding constant () between SARS-CoV-2 SP and the angiotensin-converting enzyme 2 (ACE2) receptor.

Chemical constituents, anti-rheumatic potential, and anti-inflammatory properties of the aqueous extract from the aerial parts of Aristolochia esperanzae Kuntze.

Although Aristolochia plants remain controversial due to their toxicity, this group of perianth-bearing plants, which includes the medicinal species Aristolochia esperanzae, is among the most relevant from an ethnobotanical perspective. All parts of A. esperanzae are used in popular medicine in the form of infusion for the treatment of rheumatism.

Amorphous cassava starch/spirulina protein mixtures stabilized Pickering emulsions: Preparation and stability.

This study explored stabilized emulsions using cassava starch (CS) and spirulina protein (SP) mixtures, targeting microbial proteins as potential replacements for animal proteins in food stability applications. The final viscosity and enthalpy change of the CS/SP mixtures decreased from 3.78 to 1.

Human papillomavirus genotype and cycle threshold value from self-samples and risk of high-grade cervical lesions: A post hoc analysis of a modified stepped-wedge implementation feasibility trial.

Background: Human papillomavirus (HPV) testing of self-collected vaginal samples has potential to improve coverage of cervical screening programmes, but current guidelines mostly require those HPV positive on a self-sample to attend for routine screening.

Methods And Findings: A pragmatic modified stepped-wedge implementation feasibility trial was conducted at primary care practices in England. Individuals aged 25 to 64 years who were at least 6 months overdue for cervical screening could provide a self-collected sample.

Mobility Medicine: A call to unify hyper-fragmented specialties by abstracts sent to 2025Pdm3, and typescripts to Ejtm3, and .

Mega scientific conferences increasingly suffer from the need for short and poster presentations without discussion. An alternative is to organize workshops in hotels large enough to accommodate all participants. This significantly increases the opportunities for constructive discussion during breakfasts, lunches, dinners and long evenings that can bring together experts of scientific and clinical sub-specialties and young fellows.

Longitudinal data collection in pediatric and adult patients with 5q spinal muscular atrophy in Latin America: LATAM RegistrAME study - a clinical registry study protocol.

Spinal muscular atrophy is a rare hereditary neurodegenerative disease characterized by progressive motor neuron loss. The most common form of SMA is linked to 5q (5q-SMA) and is classified into subtypes according to the age of onset and maximum motor function achieved. The severity ranges from progressive infantile paralysis and premature death (type 1) to limited motor neuron loss in adults (type 4).

The overlooked burden of persistent physical symptoms: a call for action in European healthcare.

Regardless of their cause, persistent physical symptoms are distressing somatic complaints that occur on most days for at least several months. They are common in patients with somatic diseases, functional somatic disorders, mental disorders, and undiagnosed medical conditions and are often associated with significant impairment and medical costs. Despite their prevalence and impact, persistent physical symptoms are often overlooked in medical care.

Synthesis, characterization, biological activities, and computational studies of pyrazolyl-thiazole derivatives of thiophene.

This study reports the synthesis, characterization, and biological evaluation of a series of pyrazolyl-thiazole derivatives of thiophene. Seven compounds were synthesized and characterized using NMR spectroscopy and mass spectrometry. The antimicrobial activities of these derivatives were evaluated against various bacterial (, , , ) and fungal strains (, , , ), demonstrating significant inhibition zones and low minimum inhibitory concentrations (MIC).

Secretory expression in Bacillus subtilis, purification and characterization of a persistent protein-degrading enzyme from Nocardiopsis sp. TOA-1.

Keratinase from Nocardiopsis sp. TOA-1 (NAPase) holds significant potential for industrial and medical applications. Here, we developed a heterologous secretory expression system for NAPase in Bacillus subtilis.

Australian parents' attitudes, perceptions and supply of alcohol to adolescents: a national cross-sectional survey.

Parental supply of alcohol to adolescents is associated with increased risk of subsequent adolescent alcohol use and harms, so identifying factors associated with parents' decision-making is critical. This study examined how parental supply is associated with attitudes toward adolescent alcohol use, perceived norms of parental supply, perceived behavioural control and perceived acceptable age to drink alcohol. A total of 1197 Australian parents with children aged 12-17 years completed an online cross-sectional survey assessing their parental supply behaviours, attitudes and perceptions in April 2022.

Downregulation of MLF1 safeguards cardiomyocytes against senescence-associated chromatin opening.

Aging-associated cardiac hypertrophy (AACH) increases susceptibility to heart failure in the elderly. Chromatin remodeling contributes to the gene reprogramming in AACH; however, the intrinsic regulations remain elusive. We performed a transcriptome analysis for AACH in comparison with pressure-overload-induced pathological cardiac hypertrophy in mice and identified myeloid leukemia factor 1 (MLF1) as an aging-sensitive factor whose expression was reduced during aging but could be reversed by anti-aging administrations.

Comparing Large Language Model and Human Reader Accuracy with Image Challenge Case Image Inputs.

Background Application of multimodal large language models (LLMs) with both textual and visual capabilities has been steadily increasing, but their ability to interpret radiologic images is still doubted. Purpose To evaluate the accuracy of LLMs and compare it with that of human readers with varying levels of experience and to assess the factors affecting LLM accuracy in answering Image Challenge cases. Materials and Methods Radiologic images of cases from October 13, 2005, to April 18, 2024, were retrospectively reviewed.

Evaluation of the double-zone hemolysis (DZH) test for the detection of livestock-associated methicillin-resistant .

Unlabelled: Livestock-associated methicillin-resistant (LA-MRSA), such as clonal-complex (CC)398, are of clinical relevance due to their multi-drug resistance profiles, adding to the overall burden of MRSA in humans. The objective was to evaluate the double-zone hemolysis (DZH) test as a simple and reliable method for detecting LA-MRSA in the clinical microbiology laboratory. isolates assigned to CC398 ( = 183; 152 MRSA/31 methicillin-susceptible [MSSA]), CC1 ( = 44; MRSA), and other CCs ( = 144; 94 MRSA/50 MSSA) were investigated.

Unraveling a Rare Case of Pediatric Solid Pseudopapillary Neoplasm With a Replaced Common Hepatic Artery Arising From the Superior Mesenteric Artery.

Pancreatic solid pseudopapillary epithelial neoplasm (SPEN) is a rare pancreatic tumor with low-grade malignant potential. They often present in young women in their second and third decade of life, with only a small minority concerning children. It has a good prognosis, with a five-year survival rate of up to 97%.

Biomechanical analysis of limits of stability using computerized posturography: correlations with functional mobility in elderly individuals with hip osteoarthritis - a cross-sectional study.

Background: Hip osteoarthritis (OA) is a degenerative joint disease that predominantly affects the elderly, causing significant morbidity due to joint pain, stiffness, and loss of function. This study aimed to assess the limits of stability (LOS) using computerized posturography and evaluate the correlations with functional mobility in elderly individuals with hip OA.

Methods: This cross-sectional study included elderly individuals aged 65 years and above with a clinical diagnosis of hip OA and age-matched asymptomatic controls.

[Effect of LDL-C/HDL-C ratio on severity of coronary artery disease and 2-year long-term prognosis in elderly patients with coronary heart disease].

To explore the effect of low density lipoprotein cholesterol (LDL-C)/high density lipoprotein cholesterol (HDL-C) ratio on the severity of coronary artery disease and 2-year adverse events in elderly patients with coronary heart disease. This prospective, multicenter, observational cohort study was from the prospective observational multicenter cohort for ischemic and hemorrhage risk in coronary artery disease patients (PROMISE) study, in which 18 701 patients with coronary heart disease (CHD) were included from January 2015 to May 2019. Among them, 6 770 elderly patients with CHD were enrolled in the current study.

Comparison Between Eccentric vs. Concentric Muscle Actions On Hypertrophy: A Systematic Review and Meta-analysis.

da Silva, LSL, Gonçalves, LdS, Alves Campos, PH, Benjamim, CJR, Tasinafo Júnior, MF, de Lima, LCR, Bueno Júnior, CR, and Alves, CPdL. Comparison between eccentric vs. concentric muscle actions on hypertrophy: a systematic review and meta-analysis.

Blinatumomab in Standard-Risk B-Cell Acute Lymphoblastic Leukemia in Children.

Background: B-cell acute lymphoblastic leukemia (B-cell ALL) is the most common childhood cancer. Despite a high overall cure rate, relapsed B-cell ALL remains a leading cause of cancer-related death among children. The addition of the bispecific T-cell engager molecule blinatumomab (an anti-CD19 and anti-CD3 single-chain molecule) to therapy for newly diagnosed standard-risk (as defined by the National Cancer Institute) B-cell ALL in children may improve outcomes.

Linguistic Validation of the Functional Assessment of Chronic Illness Therapy-Spiritual-Well-being-Expanded Version 4 Tool into Malayalam Language and its Feasibility in Advanced Cancer Patients Receiving Palliative Care.

Objective: The objective of this study was to validate the 'Functional Assessment of Chronic Illness Therapy-Spiritual-Well-being-Expanded(FACIT-SpEx) Version 4' tool in Malayalam and assess its feasibility among advanced cancer patients receiving palliative care.

Materials And Methods: The study was carried out at the outpatient Department of Cancer Palliative Medicine of Malabar Cancer Centre between November 2022 and June 2023. Initially, the FACIT-Sp-Ex version 4 tool with 23 items was translated into the Malayalam language with a forward-backward translation procedure.

Treatment Algorithms for Continuous Low-Level Heat Wrap Therapy for the Management of Musculoskeletal Pain: An Italian Position Paper.

Musculoskeletal pain (MSP), which impacts bones, muscles, tendons, and ligaments, is a substantial worldwide pain disorder, characterized by muscle soreness, fatigue, inflammation, muscle spasms, sleep disruptions, and functional limitations. MSP is predominantly managed within the primary care setting. Recent consensus recognizes that heat therapy (HT) may provide potential benefits, especially in treating chronic MSP.

Prolonged antithrombotic treatment after de-escalation of dual antiplatelet therapy in patients after acute coronary syndrome - which strategy should be applied? The ELECTRA-SIRIO 2 investigators standpoint.

De-escalation of dual antiplatelet (DAPT) intensity may be considered in patients with high risk of bleeding after acute coronary syndrome. Some high risk patients after de-escalation may require antithrombotic therapy prolonged over 12 months. With the current guideline recommended strategies, there are some doubts and uncertainties with respect to the transition period.