Stoichiometry design in hierarchical CoNiFe phosphide for highly efficient water oxidation.

Unlabelled: Rational composition design of trimetallic phosphide catalysts is of significant importance for enhanced surface reaction and efficient catalytic performance. Herein, hierarchical Co Ni Fe P with precise control of stoichiometric metallic elements ( = (1-10):(1-10):1) has been synthesized, and CoNiFeP, as the most optimal composition, exhibits remarkable catalytic activity ( = 320 mV at 10 mA cm) and long-term stability (ignorable decrease after 10 h continuous test at the current density of 10 mA cm) toward oxygen evolution reaction (OER). It is found that the surface P in CoNiFeP was replaced by O under the OER process.

Enhanced production of camptothecin by immobilized callus of and a bioinformatic insight into its potential antiviral effect against SARS-CoV-2.

Camptothetin (CPT) is a quinoline alkaloid originally isolated from the Chinese tree, . CPT was found to have anticancerous and antiviral properties. Derivatives of natural CPT, including topothecan and irinotecan are used clinically to treat a variety of cancers.

Microscopic analysis of the inhibition of staphylococcal biofilm formation by Escherichia coli and the disruption of preformed staphylococcal biofilm by bacteriophage.

The formation of bacterial biofilms is a severely encountered problem in clinical and industrial settings. Most of the naturally occurring bacterial strains are capable of forming mono or mixed biofilms. In this study, we evaluated the potentiality of three clinically relevant species in forming mono and mixed biofilms over glass surface.

Concerted dispersion of biofilm by bacteriophage and 'green synthesized' silver nanoparticles.

Staphylococcal biofilms predominantly cause persistent nosocomial infections. The widespread antibiotic resistance followed by its ability to form biofilm in biological and inert surfaces often contributes to major complications in patients and veterinary animals. Strategic importance of bacteriophage therapy against critical staphylococcal infections had been predicted ever since the advent of antibiotic resistant staphylococcal strains.

Functional characterization of recombinant bromelain of Ananas comosus expressed in a prokaryotic system.

Bromelain (BRM) is a defense protein present in the fruit and stem of pineapple (Ananas comosus) and it is grouped as a cysteine protease enzyme with diversified medicinal uses. Based on its therapeutic applications, bromelain has got sufficient attention in pharmaceutical industries. In the present study, the full coding gene of bromelain in pineapple stem (1,093 bp) was amplified by RT-PCR.

Determination of B cell epitopes and evaluation of antigen capture ELISA for the earlier diagnosis of CHIK virus using anti-rCHIK E1 rabbit antibodies.

Chikungunya fever caused by an alpha virus has been generally considered as self limiting and non fatal. Recent reports on Chikungunya infection indicate high mortality rates due to the severity of the viral infection. For the early diagnosis of CHIK virus, the incubation period required for the development of antibodies in the serum of patients was a constraint for antigen based ELISA.

Functional and structural variation of uridine diphosphate glycosyltransferase (UGT) gene of Stevia rebaudiana-UGTSr involved in the synthesis of rebaudioside A.

The sweetness of honey leaf plant Stevia rebaudiana is attributed to steviol glycosides or steviosides, accumulated in the leaves. Steviol glycosides are diterpenoids derived from steviol as the final step of glycosylation by the marker enzyme Uridine diphosphate glycosyltransferase (UGT). Out of the eight different steviol glycosides, rebaudioside A was detected as the sweetest glycoside with reduced bitter aftertaste.

Recombinant lactoferrin (Lf) of Vechur cow, the critical breed of Bos indicus and the Lf gene variants.

Vechur cow, categorized as a critically maintained breed by the FAO, is a unique breed of Bos indicus due to its extremely small size, less fodder intake, adaptability, easy domestication and traditional medicinal property of the milk. Lactoferrin (Lf) is an iron-binding glycoprotein that is found predominantly in the milk of mammals. The full coding region of Lf gene of Vechur cow was cloned, sequenced and expressed in a prokaryotic system.

Recombinant CHIK virus E1 coat protein of 11 KDa with antigenic domains for the detection of Chikungunya.

Chikungunya is an acute febrile illness caused by an alpha virus technically called as CHIK virus. A smaller size of CHIK virus E1 coat protein -11 kDa was expressed in prokaryotic expression system. The recombinant protein was purified and confirmed by western blot analysis.

Physicochemical characterization and functional site analysis of lactoferrin gene of Vechur cow.

The Bos indicus Vechur breed cow milk is known for its medicinal value and the breed is listed under the category of critically maintained breeds by the Food and Agriculture Organization. The lactoferrin protein in milk is known for its nutritional value. Gene polymorphisms have been reported for Bovine lactoferrin.

Rapid detection and characterization of Chikungunya virus by RT-PCR in febrile patients from Kerala, India.

There has been a resurgence and prevalence of fever with symptoms of Chikungunya (CHIK) and increased death toll in Kerala, the southern-most state of India. The objective of this study was to develop a rapid detection method to determine the presence of CHIK- virus in the serum samples collected from febrile patients in Kerala, India. Serum specimens were analyzed for CHIK viral RNA by RT-PCR using primers specific for nsP1 and E1 genes.