Revisiting ESKAPE Pathogens: virulence, resistance, and combating strategies focusing on quorum sensing.

The human-bacterial association is long-known and well-established in terms of both augmentations of human health and attenuation. However, the growing incidents of nosocomial infections caused by the ESKAPE pathogens (, , , , , and sp.) call for a much deeper understanding of these organisms.

Deep Learning-Based Skin Lesion Multi-class Classification with Global Average Pooling Improvement.

Cancerous skin lesions are one of the deadliest diseases that have the ability in spreading across other body parts and organs. Conventionally, visual inspection and biopsy methods are widely used to detect skin cancers. However, these methods have some drawbacks, and the prediction is not highly accurate.

Method for highly selective, ultrasensitive fluorimetric detection of Cu and Al by Schiff bases containing o-phenylenediamine and o-aminophenol.

Schiff base probes (1 and 2) made from o-phenylenediamine and o-aminophenol were appeared as highly selective fluorimetric chemosensor of Cu and Al ions respectively. Strong fluorescence emission of probe 1 at 415 nm (excitation at 350 nm) was instantly turned off on addition of Cu. Very weak fluorescence of probe 2 at 506 nm (excitation at 400 nm) was immediately turned on specifically by Al.

Design, synthesis and anti-Tb evaluation of chalcone derivatives as novel inhibitors of InhA.

A series of halogenated chalcone derivatives were designed and developed for anti-tubercular activity. Novel molecules were designed and screening were performed using admetSAR, SwissADME, and Osiris Property Explorer. From the initial filter the top 10 compounds were docked using the Autodock tool 1.

Multi-functional approach in the design of smart surfaces to mitigate bacterial infections: a review.

Advancements in biomedical devices are ingenious and indispensable in health care to save millions of lives. However, microbial contamination paves the way for biofilm colonisation on medical devices leading to device-associated infections with high morbidity and mortality. The biofilms elude antibiotics facilitating antimicrobial resistance (AMR) and the persistence of infections.

Design, synthesis, molecular dynamics simulation, MM/GBSA studies and kinesin spindle protein inhibitory evaluation of some 4-aminoquinoline hybrids.

The discovery of novel chemotherapeutic agents is always challenging for researchers in industry and academia. Among the recent promising anticancer therapeutic targets, an important modulatory factor in mitosis is the expression of the kinesin family motor protein (Eg5). In terms of chemotherapy treatment, mitosis has gained significant attention due to its role as one of the biological processes that can be intervened in it.

Copper, Lysyl Oxidase Activity, and Collagen in Aqueous Humour of Primary Glaucoma: An Association with Clinical Parameters.

Introduction: To measure copper (Cu), lysyl oxidase (LOX) activity, and collagen levels in aqueous humour (AH) of primary glaucoma patients and correlate with clinical parameters.

Methods: 120 patients with 40 each of primary open angle glaucoma (POAG), primary angle closure glaucoma (PACG), and cataract controls were recruited in this case-control study. AH samples were collected during the trabeculectomy and cataract surgeries.

Viable remediation techniques to cleansing wastewaters comprising endocrine-disrupting compounds.

Endocrine-disrupting chemicals (EDCs) have recently gained prominence as emerging pollutants due to their significant negative impacts on diverse living forms in ecosystems, including humans, by altering their endocrine systems. EDCs are a prominent category of emerging contaminants in various aquatic settings. Given the growing population and limited access to freshwater resources, their expulsion from aquatic systems is also a severe issue.

Amalgamation of nanotechnology with chicken IgY to enrich therapeutic and diagnostic applications: a systematic review.

The article explores the possibility of using nanoparticles and IgY technology together for biosensing and antibody delivery to fight mammalian infections. The use of IgG in passive immunotherapy has drawbacks; however, nanoparticles and IgY technology offer new opportunities for diagnostic and therapeutic applications. The primary selection of reports was based on the title and abstract, and potential studies were selected based on predefined inclusion criteria such as nanoparticle/nanomaterials and IgY, studies that have employed nanoparticles-IgY for diagnostic and therapeutic applications and animal experiments.

Citizen science facilitates first ever genetic detection of wolf-dog hybridization in Indian savannahs.

Human demographic expansion has confined wildlife to fragmented habitats, often in proximity to human-modified landscapes. Such interfaces facilitate increased interactions between feral or domesticated animals and wildlife, posing a high risk to wild species. This is especially relevant for free-ranging dogs () and wild canids like gray wolves () and golden jackals ().

Effect of osmolytes on the EcoRI endonuclease: Insights into hydration and protein dynamics from molecular dynamics simulations.

Osmolytes play an important role in cellular physiology by modulating the properties of proteins, including their molecular specificity. EcoRI is a model restriction enzyme whose specificity to DNA is altered in the presence of osmolytes. Here, we investigate the effect of two different osmolytes, glycerol and DMSO, on the dynamics and hydration of the EcoRI enzyme using molecular dynamics simulations.

Development of an integrated telerehabilitation model in children with cerebral visual impairment in South India during the COVID-19 pandemic - A pilot study.

Purpose: Coronavirus disease 2019 (COVID-19) pandemic affected the in-person rehabilitation/habilitation services in families with children with cerebral visual impairment (CVI) in India. This study aimed to develop a structured and family-centered telerehabilitation model alongside conventional in-person intervention in children with CVI to observe its feasibility in the Indian population.

Methods: This pilot study included 22 participants with a median age of 2.

The concept of Big Four: Road map from snakebite epidemiology to antivenom efficacy.

Snake envenomation is a life-threatening disease caused by the injection of venom toxins from the venomous snake bite. Snakebite is often defined as the occupational or domestic hazard mostly affecting the rural population. India experiences a high number of envenoming cases and fatality due to the nation's diversity in inhabiting venomous snakes.

Removal of cationic and anionic toxic pollutants from simulated solutions using pod (SFP): equilibrium isotherm, kinetics, and characterization.

The present work explores the sorption performance of pod (SFP) for the removal of methylene blue (MB) and chromium (Cr) from simulated solutions separately. The material characteristics namely textural analysis (specific surface area: 2.45 m/g), morphological behavior (heterogeneous morphology containing pores and cavities), functional analysis (COO- stretching, C-O-C stretching vibrations, and -OH stretching) and thermal behavior (279.

Utilization of Bioinorganic Nanodrugs and Nanomaterials for the Control of Infectious Diseases Using Deep Learning.

As one of the main causes of morbidity and mortality, viral infections have a major impact on the well-being and economics of every nation in the globe. The ability to predictably diagnose viral infections improves the provision of good healthcare as well as the control and prevention of these conditions. Nanomaterials have gained widespread usage in the medical industry recently due to the rapid advancement of nanotechnology and their exceptional chemical and physical qualities, such as their small size and synthesized surface properties.

Key essential oil components delocalize Candida albicans Kar3p and impact microtubule structure.

Background: Treatment of Candida albicans associated infections is often ineffective in the light of resistance, with an urgent need to discover novel antimicrobials. Fungicides require high specificity and can contribute to antifungal resistance, so inhibition of fungal virulence factors is a good strategy for developing new antifungals.

Objectives: Examine the impact of four plant-derived essential oil components (1,8-cineole, α-pinene, eugenol, and citral) on C.

Unwinding the mechanism of macrophage repolarization potential of sp. -derived protein protease inhibitor through Toll-like receptor 4 against experimental visceral leishmaniasis.

The sp. -derived protein protease inhibitor (PPI) has been proven to shift macrophages towards an inflammatory state and reduce infection and . The current study explored and validated the mechanistic aspects of the PPI and Toll-like receptor (TLR) interaction.

Crustacean polysaccharides for the geotechnical enhancement of organic silt: A clean and green alternative.

Biopolymer-based soil stabilization offers a clean alternative to conventional stabilizers like cement and lime. This study investigates the possibility of using shrimp-based chitin and chitosan for stabilizing low plastic silt with organic content by investigating their effect on pH, compaction, strength, hydraulic conductivity (HC) and consolidation characteristics. X-ray diffraction (XRD) spectrum shows that no new chemical compounds were formed in the soil on additive treatment; however, results of scanning electron microscope (SEM) analysis indicate the formation of biopolymer threads that bridge the voids in the soil matrix leading to a stiffer soil matrix, with increased strength and lower HC.

Feature Extraction Using a Residual Deep Convolutional Neural Network (ResNet-152) and Optimized Feature Dimension Reduction for MRI Brain Tumor Classification.

One of the top causes of mortality in people globally is a brain tumor. Today, biopsy is regarded as the cornerstone of cancer diagnosis. However, it faces difficulties, including low sensitivity, hazards during biopsy treatment, and a protracted waiting period for findings.

Antimicrobial peptides derived from microalgae for combating antibiotic resistance: Current status and prospects.

Microalgae are photosynthetic cell factories that produce a spectrum of bioactive compounds extensively used for various applications. Owing to the increase in antibiotic resistance among microbial pathogens, there is a significant thrust for identifying new treatment strategies, and antimicrobial peptides (AMPs) generation is one such method. These AMPs have multiple roles and are active against bacteria, fungi, and viruses.

In silico identification of a promising inhibitor of Fusarium oxysporum f. sp. Lycopersici, Secreted in Xylem 1 protein.

Fusarium oxysporum f. sp. Lycopersici (FOL) is a soilborne pathogen that infects tomato plants and inflicts severe damage, resulting in heavy yield losses worldwide, causing Fusarium wilt disease.

Parental perception of facilitators and barriers to activity and participation in an integrated tele-rehabilitation model for children with cerebral visual impairment in South India - A virtual focus group discussion study.

Purpose: This study utilized virtual focus group discussions to document the facilitators and barriers reported by the parents as part of the tele-rehabilitation service delivery model in India.

Methods: This study included 17 participants who were enrolled into the Tele-rehabilitation program (16 mothers, 1 father) and the virtual focus group discussion (V-FGD) were conducted through a WhatsApp video call. Three V-FGDs were conducted involving two moderators and a note taker.

Development of a smart pH-responsive nano-polymer drug, 2-methoxy-4-vinylphenol conjugate against the intestinal pathogen, Vibrio cholerae.

Vibrio cholerae causes cholera, an acute diarrhoeal disease. The virulence in V. cholerae is regulated by the quorum-sensing mechanism and response regulator LuxO positively regulates the expression of virulence determinants adhesion, biofilm formation, and cholera toxin production.

Structural and functional mapping of gene cluster in DR1.

DR1 is a novel Gram-negative bacterium, isolated from the Dadri wetlands in Uttar Pradesh, India. In addition to being radiation-resistant, the rod-shaped, red-pigmented organism shows extraordinary resistance to arsenic. The proteins of the corresponding gene cluster involved in arsenic extrusion in DR1 have not yet been characterized.