Upregulation of Urinary Alpha-2-HS Glycoprotein During Pregnancy of Murrah Buffaloes.

Urine samples were systematically collected from inseminated Murrah buffaloes (Bubalus bubalis) on days 0, 7, 14, 21 and 28 (with day 0 representing the day of artificial insemination). Following confirmation of pregnancy via trans rectal palpation 45 days of insemination, the animals were categorised into pregnant and non-pregnant groups (n = 10 each). The urine samples on 0, 7, 14, 21 and 28 days of pregnant and one sample from non-pregnant preferably collected on 28th day was used for SDS-PAGE after diafiltration.

Kisspeptin stimulates sheep ovarian follicular development through homologous receptors.

The present study was conducted to elucidate (1) the influence of kisspeptin (KP) on the development of preantral follicles (PFs) and (2) evolution of KP receptor gene () expression during ovarian follicular development in sheep. Kisspeptin was supplemented (0-100 µg/ml) in the culture medium of PFs for 6 days. The cumulus-oocyte complexes (COCs) from cultured PFs were subsequently matured to metaphase II (MII) for an additional 24 h.

Digital infrared thermal imaging of udder skin surface temperature: a novel non-invasive technology to monitor calving process in Murrah buffalo (Bubalus bubalis).

Quantifiable decline in the maternal body temperature during the pre-calving offers the possibilities for predicting the calving that can improve the calving management. As infrared thermography (IRT) is a simple non-contact tool for precise measurement of surface temperature, we investigated the use of IRT to establish thermal signatures around calving in the Murrah buffalo. The IRT of eye, right lateral, left lateral and rear side of udder skin surface temperature (USST) were recorded at 6 h interval from 96 h before the expected date of calving, at the time of calving and 24 h post-calving in Murrah buffaloes (n = 28).

Single nucleotide polymorphism in STAT5A could not endorse variation in milk production traits in Indian bovine population.

The Signal Transducer and Activator of Transcription 5A (STAT5A) gene involved in activating the transcription of milk protein genes was predicted to be influencing milk production traits. The present study was undertaken to investigate the suitability of the polymorphism of STAT5A as a marker for milk traits in Ongole, crossbred cattle and Murrah buffaloes from Southern India. Blood samples (n = 502) for DNA isolation and milk samples (n = 222) from different genetic groups were collected from various farms.

Leptin induced in vitro development of ovarian follicles in sheep is related to the expression of P450 aromatase and steroidogenesis.

The objective of this study was to test the hypothesis that Leptin induced in vitro growth in preantral follicles in sheep involves modulation of P450 aromatase expression and steroidogenesis. Accordingly, the expression of P450 aromatase gene was studied in the cumulus cells and oocytes isolated from different stages of preantral follicles (PFs') grown in vivo, cultured in TCM 199B, TCM 199B + Leptin (10 ng/ml) (TCM199BL) or a standard PF culture medium supplemented with Leptin (10 ng/ml) (SML). Ovarian follicles grown in vivo or in SML expressed P450 aromatase both in cumulus cells and oocytes at all the development stages.

Expression of kit ligand and insulin-like growth factor binding protein 3 during in vivo or in vitro development of ovarian follicles in sheep.

Expression of Kit ligand (KL) and insulin-like growth factor binding protein (IGFBP3) genes was studied at different in vivo and corresponding in vitro stages of development of the ovarian follicles in sheep. The expression of both KL and IGFBP3 was significantly higher in the primordial follicles relative to any other stage of development. Compared to the other stages, the KL expression in the cumulus cells from in vivo grown large antral follicles and that of IGFBP3 in COCs' isolated from large antral follicles matured in vitro for 24 hr were significantly higher.

In vitro evaluation of acaricidal activity of novel green silver nanoparticles against deltamethrin resistance Rhipicephalus (Boophilus) microplus.

An investigation was undertaken to study, for the first time, in vitro acaricidal activity of green silver nanoparticles on deltamethrin resistance Rhipicephalus (Boophilus) microplus. The compounds tested were neem coated silver nanoparticles (N-Ag NPs), deltamethrin neem coated silver nanoparticles (DN-Ag NPs), 2, 3 dehydrosalannol (2,3 DHS), 2, 3 DHS coated silver nanoparticles (2, 3-DHS-Ag NPs), Quercetin dihydrate (QDH) and QDH coated silver nanoparticles (QDH-Ag NPs). Also included in this study, for the purpose of comparison, were neem leaf extract (NLE), silver nitrate (AgNO) and deltamethrin (D).

Occurrence of and in backyard poultry in India.

Birds reared in backyard poultry farming system are more prone to parasitic infestation resulting in economic losses to rural community. The present study reports occurrence of and mites in a male Aseel bird. Clinical signs observed in the affected bird include hyperkeratosis with whitish film layer on shank and hock region of legs.

Seasonal prevalence of gastrointestinal parasites in desi fowl (Gallus gallus domesticus) in and around Gannavaram, Andhra Pradesh.

A study was carried out to know the prevalence of gastrointestinal parasites in desi fowl in and nearby villages of Gannavaram, Andhra Pradesh for a period of 1 year. Screening of 492 samples comprising faecal samples and gastrointestinal tracts from freshly slaughtered desi birds at local poultry shops and samples from post mortem examinations at NTR College of Veterinary Science, Gannavaram revealed 63.21 % of gastrointestinal parasites.

Effect of selenium supplementation on performance, cost economics, and biochemical profile of Nellore ram lambs.

Aim: Present experiment was conducted to investigate the effect of selenium (Se) supplementation on performance, carcass characteristics, meat composition, shelflife of meat and biochemical profile in Nellore ram lambs.

Materials And Methods: 24 male Nellore ram lambs (15.75±0.

Effect of leptin on in vitro development of ovine preantral ovarian follicles.

The influence of human or ovine leptin on in vitro culture of preantral follicles (PFs) isolated from sheep ovaries was investigated. Among the 12 different concentrations (0-1000 ng/mL) of human leptin tested, proportion of PFs exhibiting growth, mean increase in diameter, antrum formation, and maturation of the oocytes to MII stage were the best in 10 ng/mL. Culture of sheep ovarian PFs in TCM 199 supplemented with 10 ng/mL of human or ovine leptin FSH (2.

Effect of feeding sweet sorghum stover-based complete rations on the growth performance and carcass characteristics of ram lambs.

The present study was carried out to evaluate the effect of sweet sorghum stover (cost $0.05/kg) supplementation as complete balanced diet (at 60 % level) on sheep performance, carcass characteristics and economics in comparison to maize stover (cost $0.08/kg) and sorghum stover (cost $0.

Molecular Typing of Bluetongue Viruses Isolated Over a Decade in South India.

Bluetongue (BT) is an arthropod-borne viral disease mostly of sheep. Bluetongue virus (BTV) is a segmented double-stranded RNA virus belonging to the genus Orbivirus of family Reoviridae and is transmitted by midges belonging to Culicoides spp. The disease is endemic in the tropics and subtropics, and the incidence is high in southern India.

Quantitative expression of antiapoptotic and proapoptotic genes in sheep ovarian follicles grown in vivo or cultured in vitro.

To test the hypothesis that the poor development of the oocytes in cultured ovarian follicles of mammals is due to aberrant expression of developmentally important genes, quantitative expression patterns of Bcl2 (B-cell leukemia/lymphoma 2; antiapoptotic) and Bax (Bcl2-associated X protein; proapoptotic) genes in preantral, early antral, antral, large antral follicles, and cumulus-oocyte complexes (COCs) grown in vivo or cultured in vitro were studied. The level and pattern of expression of Bcl2 in the cumulus cells isolated from different development stages of in vivo- and in vitro-grown ovarian follicles were similar suggesting that in vitro culture did not alter the expression of this antiapoptotic gene in the cumulus cells. However, between the in vivo- and in vitro-grown ovarian follicles (1) Bcl2 expression levels in the oocytes from antral follicles (2.

Growth and Nutrient Utilization in Kids Fed Expander-extruded Complete Feed Pellets Containing Red Gram (Cajanus cajan) Straw.

A growth and digestibility study was conducted using Osmanabadi goat male kids by feeding complete diets in the form of mash or expander extruded pellets containing different levels of red gram (Cajanus cajan) straw (RGS). Two iso-nitrogenous complete diets were prepared by incorporating RGS at 35% and 50% levels. Half the quantity of each complete mash feed was then converted into pellets through expander extruder processing.

Growth performance and carcass characteristics of growing ram lambs fed sweet sorghum bagasse-based complete rations varying in roughage-to-concentrate ratios.

Different roughage-to-concentrate ratios of sweet sorghum bagasse (SSB) (a by-product of the biofuel industry)-based complete diets were assessed. Twenty four growing Nellore × Deccani ram lambs aged about 3 months (average body wt., 10.

Effect of roughage to concentrate ratio of sweet sorghum (Sorghum biclor L. Moench) bagasse-based complete diet on nutrient utilization and microbial N supply in lambs.

An experiment was conducted to study the effect of roughage to the concentrate ratio of complete diets containing sweet sorghum bagasse (SSB), an agro-industrial by product, as sole roughage source on nutrient utilization in ram lambs. Twenty-four Nellore × Deccani cross ram lambs aged about 3 months (average body wt. 10.