Preparation of mixed keratinocyte and melanocyte cultures from biopsy specimens of pigmented skin sites of patients with vitiligo.

A method for preparation and expansion of mixed keratinocyte and melanocyte culture from human skin biopsy specimens was developed. The culture contains two melanocyte types: derivatives of hair follicle stem cells and epidermal basal layer stem cells. Fibroblasts were completely eliminated after culturing in selective medium.

Study of the dynamics of Blastocystis hominis reproduction in vitro.

The dynamics of Blastocystis hominis reproduction in vitro in Pavlova's and Nelson-Jones media was studied. The time of generation in these media was 21.5 and 16.

Activity of antioxidant enzymes in the wound in patients with deep burns.

Analysis of wound discharge in children with deep burns over 3 weeks after the injury revealed gradual increase in catalase activity. The increase in activities of myeloperoxidase, glutathione-S-transferase, and catalase was maximum in patients with the most severe burns. Local complications were observed during the period of maximum myeloperoxidase activity, while the beginning of epithelialization was associated with its reduction.

Ultrastructural characteristics of peripheral arteriovenous and venous angiodysplasias.

Comparative histological and electron microscopic study of arteriovenous and venous angiodysplasias revealed specific features of their structure, presumably reflecting differences in their morphogenesis. Specific ultrastructural characteristics of angiodysplasias are modified shape of endotheliocytes, impaired structure of the basal membrane, and reduced count of pericytes.

Chronotropic and inotropic components of cardiac reflexes in cats.

The relationship between chronotropic and inotropic components of cardiac reflexes were studied in cats using intravenous blood injections, occlusion of the abdominal aorta, pressing on eyeballs (Aschner maneuver), and occlusion of the carotid arteries. Inotropic reactions were characterized by the contractility index DPxHR/MSAP. Different reflexes were shown to be characterized by different ratio of chronotropic and inotropic components, in addition, heart rate and contractility were often altered in opposite directions.

[Estrogen receptors in the diagnosis and treatment of hormone-dependent tumors].

The paper presents the current data available on the etiology of tumor diseases, among other things, shows the role of hormones, namely: estrogens and their receptors, in the mechanism responsible for the occurrence of these diseases. Each tumor was shown to have its receptor status and to be unresponsive to this or that therapy differently. The efficiency of treatment and its prognosis may be determined if the receptor composition of an involved organ is estimated correctly.

Depressive disorders in stroke patients.

Post-stroke depression is a serious complication of ischemic stroke which has negative influences on patients' quality of life and on recovery from symptoms and the prognosis of the underlying disease. We report here a study of the efficacy of treatment of patients with post-stroke depression with Opra, showing improvements in status in 93.3% of patients.

Tobramycin for inhalation in cystic fibrosis: Beyond respiratory improvements.

European consensus guidelines recommend nebulised antibiotics for maintenance therapy in patients with cystic fibrosis and chronic Pseudomonas aeruginosa infection. Two formulations of tobramycin for inhalation are available in Europe (Tobi; Novartis AG, Switzerland; Bramitob; Chiesi Farmaceutici S.p.

Effect of serotonin on impulse activity of bulbar cardiovascular neurons.

Alteration of impulse activity of afferent and intercalatory neurons of bulbar cardiovascular center upon serotonin microinjection was demonstrated in acute experiments in rabbit. Afferent neurons were more sensitive to serotonin than interneurons.

Comparison of biomechanical properties of light mesh endoprostheses with different structural characteristics.

Three types of light mesh endoprostheses with different jersey structure were implanted into the anterior abdominal walls of 18 rabbits. Changes in the geometrical size and mechanical properties of the prostheses detected 3 months after implantation largely depended on the jersey structure and distribution of mature connective tissue in the structure of the material.

Search for protein adhesin gene in Bifidobacterium longum genome using surface phage display technology.

A fragment of the nucleotide sequence encoding polypeptide binding to HT-29 epithelial cells was cloned from VMKB44 Bifidobacterium longum genome library using surface phage display technology. Sequencing of this polypeptide consisting of 26 amino acid residues showed that it is an extracellular fragment of a large BL0155 transmembrane protein belonging to the ABC transport protein superfamily. The genes encoding homologues of this protein were detected in genomes of not only bifidobacteria of different species, but also in many other enteric commencals and pathogens.

Use of Noben (idebenone) in the treatment of dementia and memory impairments without dementia.

Noben (idebenone) at a dose of 120 mg per day for six months was used in the treatment of 35 patients aged 60-86 years with Alzheimer's-type dementia, mixed dementia, and memory impairments not reaching the stage of dementia. Patients were assessed on the basis of data from somatic, neurological, and psychiatric investigations, as well as neuropsychological testing and a series of psychometric and other scales and tests, before and after treatment. Significant improvements in patients' conditions on the MMSE were seen in patients with mild and moderate dementia.

Sequelae of closed craniocerebral trauma and the efficacy of piracetam in its treatment in adolescents.

The efficacy of piracetam in treating the sequelae of moderate and severe closed craniocerebral trauma (CCT) in adolescents was evaluated in studies of 42 patients aged 12-18 years who had suffered CCT 1.5-5 years prior to the study. Adolescents of the experimental group (20 individuals) received piracetam (Nootropil) at doses of 40-50 mg/kg (daily daily 1600-2400 mg) for one month; patients of group 2 (22 individuals) served as controls.

Adjunctive use of tafluprost with timolol provides additive effects for reduction of intraocular pressure in patients with glaucoma.

Purpose: This study investigated the efficacy and safety of tafluprost as an adjunctive therapy to timolol in patients with open-angle glaucoma or ocular hypertension, uncontrolled by timolol monotherapy.

Methods: This was a randomized, double-masked, parallel-group, multinational and multicenter 12-week phase III study. Tafluprost 0.

Rheolytic thrombectomy: possibilities and first results.

The present study was undertaken to investigate the possibilities of using the method of rheolytic thrombectomy (hereinafter referred to as RTE) in surgical practice, and to assess its immediate outcomes in treatment of thromboses of arteries and veins of the lower extremities, as well as transjugular intrahepatic portosystemic shunts (hereinafter referred to as TIPS). We have gained experience in carrying out a total of 33 rheolytic thrombectomies with the help of the system JET 9000 (R) using the Xpeedior catheter in a total of thirty-one 43-to-87-year-old patients. Twenty-four interventions were performed for acute thromboses of arteries and shunts of the lower limbs, five - in patients presenting with floating thrombi in the inferior vena cava, and four operations were carried out in patients diagnosed with thrombosis of the segment after TIPS.

Experimental study of the impact of the textile structure of mesh endoprostheses for the efficiency of reconstruction of the anterior abdominal wall.

Endoprostheses made from knitted fabric of 3 loop types were used for hernioplasty in rats. Biocompatibility of implanted textile constructions was evaluated after 6 months. The intensity of inflammatory reaction and types of complications in animals depended on the loop type and method of finishing of the mesh endoprosthesis tissue.

Recent advances in rehabilitation of stroke survivors.

This report discusses the newest approaches to rehabilitation of post-stroke patients. Recent studies have clinical implications for the treatment of stroke at all stages, and chronic aphasia.

Development and introduction of production standards for cell products of mesenchymal origin.

The results of the development and introduction of universal production standards for cell products of mesenchymal origin are presented: technology for obtaining and culturing of primary cell cultures from human postnatal organs and tissues, cell product quality and safety control procedures, methods for cell product storage and transportation, and the necessary files.

Effect of atropine, propranolol, and atenolol on wave structure of heart rate oscillations in rats.

Were studied the effects of atropine and beta-adrenoblockers on the power of very low (<0.25 Hz), low (0.25-0.

Evaluation of cytochrome c affinity to anionic phospholipids by means of surface plasmon resonance.

We attempted to evaluate the affinity of the anionic phospholipids to cytochrome c by means of surface plasmon resonance (SPR) technique and to correlate it with the cytochrome c active site alterations and peroxidase activity. Our experiments showed a strong interdependence between the phospholipid fatty acid saturation degree, the active site structure alterations and peroxidase activity of the cytochrome c phospholipid complex. Cytochrome c peroxidase activity and Trp59 fluorescence increase in the sequence of phosphatidyl choline (PC)-->phosphatidylserine (PS)-->cardiolipin (CL)-->phosphatidic acid (PA).

Pathomorphology of myocardial circulation: comparative study in increased left or right ventricle afterload.

Comparative study of pathomorphology of myocardial circulation under conditions of increased afterload of the left or right ventricles showed similar changes. All compartments of the coronary bed were plethoric, capillary blood stasis and perivascular edema, more pronounced in arterial vessels, were detected in both cases. These changes equally involved both ventricles and the ventricular septum.

Use of bone marrow mesenchymal (stromal) stem cells in experimental ischemic stroke in rats.

The effects of human mesenchymal stem cells on neurological functions and behavioral reactions of animals and on damaged brain tissue were studied on the model of focal cerebral ischemia in rats. Homing and differentiation of transplanted mesenchymal stem cells were also studied. Significant regression of neurological disorders after cell transplantation was noted, no appreciable shifts were detected by magnetic resonance tomography.

Activity lysosomal enzymes in rat skin fibroblasts after treatment with progesterone and new gestagen ABMP.

The effects of a new synthetic gestagen 17alpha-acetoxy-3beta-butanoyloxy-6-methyl-pregna-4,6-dien-20-on (ABMP) and reference drug progesterone on rat skin fibroblasts were evaluated by variations in lysosomal enzyme activity (cathepsin D and beta-glucosidase). Our results suggest that ABMP exhibits lysosomotropic properties, which depended on its concentration and time of treatment. The direct effect of progesterone on lysosomal enzyme activity in skin fibroblasts was compared to the influence of systemic treatment with gestagens on skin lysosomes.

In vitro comparison of immunological properties of cultured human mesenchymal cells from various sources.

Cell-mediated cytotoxicity was studied in vitro during the interaction of bone marrow mesenchymal stem cells, fibroblast-like cells from newborn umbilical cord, and skin fibroblasts of an adult donor with peripheral blood mononuclear cells. Independently on the origin, mesenchymal cells were not lysed with allogeneic natural killer cells and cytotoxic lymphocytes. Mixed cultures of mesenchymal cells had no cytotoxic effect on peripheral blood mononuclear cells and did not activate proliferation of T and B lymphocytes, natural killer cells, and CD14+ lymphocytes.