The pursuit of linear dosage in pharmacy: reservoir-based drug delivery systems from macro to micro scale.

Introduction: The pursuit of linear dosage in pharmacy is essential for achieving consistent therapeutic release and enhancing patient compliance. This review provides a comprehensive summary of zero-order drug delivery systems, with a particular focus on reservoir-based systems emanated from different microfabrication technologies.

Areas Covered: The consideration of recent advances in drug delivery systems is given to encompass the key areas including the importance of achieving a constant drug release rate for therapeutic applications.

Blood plasma proteomic markers of Alzheimer's disease, current status and application prospects.

Introduction: Identifying early risks of developing Alzheimer's disease (AD) is a major challenge as the number of patients with AD steadily increases and requires innovative solutions. Current molecular diagnostic modalities, such as cerebrospinal fluid (CSF) testing and positron emission tomography (PET) imaging, exhibit limitations in their applicability for large-scale screening. In recent years, there has been a marked shift toward the development of blood plasma-based diagnostic tests, which offer a more accessible and clinically viable alternative for widespread use.

WhiA transcription factor provides feedback loop between translation and energy production in a genome-reduced bacterium.

Introduction: WhiA is a conserved protein found in numerous bacteria. It consists of an HTH DNA-binding domain linked with a homing endonuclease (HEN) domain. WhiA is one of the most conserved transcription factors in reduced bacteria of the class Mollicutes.

Isolation and characterization of cellulose-mineralizing haloalkaliphilic bacteria from Siberian soda lakes.

Soda lakes are unique double-extreme habitats characterized by high salinity and soluble carbonate alkalinity, yet harboring rich prokaryotic life. Despite intensive microbiology studies, little is known about the identity of the soda lake hydrolytic bacteria responsible for the primary degradation of the biomass organic matter, in particular cellulose. In this study, aerobic and anaerobic enrichment cultures with three forms of native insoluble cellulose inoculated with sediments from five soda lakes in south-western Siberia resulted in the isolation of four cellulotrophic haloalkaliphilic bacteria and their four saccharolytic satellites.

Attempts to preserve and visualize protein corona on the surface of biological nanoparticles in blood serum using photomodification.

A protein corona is present on any nanoparticle (NP) entering biological fluids; however, the existence of a natural protein corona on natural NPs has not been experimentally confirmed. We used our previously developed photomodification method to fix the natural corona on "biological nanoparticles" (bio-NPs) in fetal bovine serum and newborn bovine serum; native sera served as a control. To isolate photomodified bio-NPs, we used ultracentrifugation (UC), sucrose gradient (12%, 30%, and 50%), and sucrose cushion (30%) methods.

Polyamine Adducts with AP Sites: Interaction with DNA Polymerases and AP Endonucleases.

Biological polyamines, such as spermine, spermidine, and putrescine, are abundant intracellular compounds mostly bound to nucleic acids. Due to their nucleophilic nature, polyamines easily react with apurinic/apyrimidinic (AP) sites, DNA lesions that are constantly formed in DNA by spontaneous base loss and as intermediates of base excision repair. A covalent intermediate is formed, promoting DNA strand cleavage at the AP site, and is later hydrolyzed regenerating the polyamine.

Continuum elasticity and molecular dynamics of a pore in archaeal bolalipid membranes.

are famous for their ability to survive in extremely harsh environments, probably due to the unprecedented stability of their lipid membranes. Key features of archaeal lipids (bolalipids) that confer their stability are methyl side groups and cyclopentanes in the alkyl chains, as well as the specific shape of the molecule, which has two headgroups connected by two tails. However, the contribution of each structural parameter to membrane stability and the underlying physical mechanism remain unknown.

Evaluation of the efficacy of natural orifice specimen extraction surgery versus conventional laparoscopic surgery for colorectal cancers: A systematic review and meta-analysis.

Aim: Natural orifice specimen extraction surgery (NOSES) has gained significant importance in treating cancers. The current study is a meta-analysis that aimed to assess the short-term efficacy and long-term prognostic impact of NOSES and conventional laparoscopic (CL) surgery in the treatment of colorectal cancer (CRC).

Method: Published reports in several medical databases up to February 2024 were searched and information pertinent to outcomes of NOSES and CL in retrospective and randomized studies to treat CRC was collected.

Applications of Indocyanine Green in Breast Cancer for Sentinel Lymph Node Mapping: Protocol for a Scoping Review.

Intro: Breast cancer is the leading cause of morbidity and mortality worldwide. Accurate sentinel lymph node (SLN) mapping is crucial for staging and treatment planning in early-stage breast cancer. Indocyanine green (ICG) has emerged as a promising agent for fluorescence imaging in SLN mapping.

1D CoMoC-Based Heterojunctional Nanowires from Pyrolytically "Squeezing" PMo/ZIF-67 Cubes for Efficient Overall Water Electrolysis.

The bi-transition-metal interstitial compounds (BTMICs) are promising for water electrolysis. The previous BTMICs are usually composed of irregular particles. Here, this work shows the synthesis of novel 1D CoMoC-based heterojunction nanowires (1D Co/CoMoC) with diameters about 50 nm and a length-to-diameter ratio about 20 for efficient water electrolysis.

Genomic typing, antimicrobial resistance gene, virulence factor and plasmid replicon database for the important pathogenic bacteria Klebsiella pneumoniae.

Background: The infections of bacterial origin represent a significant problem to the public healthcare worldwide both in clinical and community settings. Recent decade was marked by limiting treatment options for bacterial infections due to growing antimicrobial resistance (AMR) acquired and transferred by various bacterial species, especially the ones causing healthcare-associated infections, which has become a dangerous issue noticed by the World Health Organization. Numerous reports shown that the spread of AMR is often driven by several species-specific lineages usually called the 'global clones of high risk'.

SequenceCraft: machine learning-based resource for exploratory analysis of RNA-cleaving deoxyribozymes.

Background: Deoxyribozymes or DNAzymes represent artificial short DNA sequences bearing many catalytic properties. In particular, DNAzymes able to cleave RNA sequences have a huge potential in gene therapy and sequence-specific analytic detection of disease markers. This activity is provided by catalytic cores able to perform site-specific hydrolysis of the phosphodiester bond of an RNA substrate.

Activation of Microglia and Astroglia in Unilateral Focal Traumatic Brain Injury in Rats.

The number of microglia cells and astrocytes in layer V of the cerebral cortex was estimated on day 7 after damage caused by a unilateral focal traumatic brain injury of the left hemisphere sensorimotor cortex. Quantitative assessment was performed by counting immunocytochemically stained microglia cells (Iba1 marker) and activated astrocytes (GFAP) at different distances from the lesion site. Activation of microglial and astroglial cells was observed not only in the marginal zone of the lesion of the left hemisphere, but also in the intact hemisphere.

Influence of Urinary Urea Concentration on Hemolytic Activity and Cytotoxicity of Uropathogenic Morganella morganii Strain.

We studied the effect of urinary urea concentration on the hemolysin production and cytotoxicity of the uropathogenic Morganella morganii strain MM 190. The highest hemolytic activity of M. morganii cultivated in urine with low urea concentration (23 and 82 mmol/liter) was observed between 3rd and 4th hours of post-inoculation, while in urine with standard urea level (117 mmol/liter), the activity was observed at 5th hour of post-inoculation.

Micromycetes of the Genus Alternaria Are Producers of Emerging Mycotoxins: Analysis of Profile and Toxinogenic Potential In Vitro.

Micromycetes from the genus Alternaria are commonly found in plant food raw materials, and their produced emerging mycotoxins (EMT) pose a risk to human health. Based on polyphase taxonomy, we studied the species composition of the Alternaria spp. population in samples of Russian grain and berries; non-toxinogenic species of Alternaria of the Infectoriae section and toxinogenic species of the Alternaria section were found.

Changes in Functional Activity of Platelets under the Influence of Submicron Particles Based on Mesoporous Silica and pH-Sensitive Polymers.

In vitro and in vivo effects of mesoporous silica nanoparticles (MSN) on the functional activity of platelets were studied in experiments on white rats. MSN particles, neither uncoated nor coated with calcium alginate, induced spontaneous platelet aggregation when added to platelet-rich plasma, but significantly enhanced ADP-induced platelet aggregation. Subcutaneous administration of uncoated and calcium alginate-coated MSN resulted in increased maximum size and rate of platelet aggregate formation 1 day post-injection.

Microbiota of the Mammary Gland in Wistar Rats with Chemically Induced Breast Cancer after Treatment.

We conducted a comparative study of the mammary gland microbiota in female Wistar rats and the microbiota associated with breast cancer (BC) induced by the administration of N-methyl-N-nitrosourea, after surgical treatment, photodynamic therapy (PDT), and chemotherapy (CT). Selective nutrient media and a smear-fingerprint technique were used to study the microbiota. Staphylococcus, Streptococcus, and Lactobacillus were found in the mammary glands of intact rats.

Complex of Angiotensin II with Modified Low-Density Lipoproteins: Behavioral and Hemodynamic Effects in Rats.

Endogenous multiple modified LDL (mLDL) and the renin-angiotensin system play a significant role in the development of atherosclerosis. It has been found that by behavioral and hemodynamic parameters the physiological activity of angiotensin II (Ang II) in combination with mLDL is considerably modified due to weakening of its diuretic effect and the inversion of hypertensive and tachyarrhythmic effects. Atherosclerosis is a long-term pathological process, so a single administration of artificially synthesized Ang I-mLDL complexes can be considered a model of the first contact of the body with pathogenic factors.

Production of the Key Immunoregulatory Cytokines and the Content of FoxP3 T-Regulatory Lymphocytes in the Epicardial and Thymus Adipose Tissue in Patients with Coronary Heart Disease.

FoxP3 T-regulatory (Treg) lymphocytes and cytokine production by cells from the stromal vascular fraction (SVF) of epicardial (EAT) and thymus (TAT) adipose tissue of 42 patients with chronic coronary heart disease (CHD) were studied. In the SVF of TAT in patients with Gensini Score (GS)≥74 (the most severe atherosclerosis), the production of IL-1β, TNF, IL-4, and IFNγ was higher, while FoxP3 translocation into the nucleus was lower than in patients with GS<74. The GS index directly correlated with the production of IL-4, IL-1β, and TNF by cells of the SVF of TAT, and inversely - with the production of TNF, IL-17, and IL-10 by cells of the SVF of EAT.

The Effect of Rhythmic Optical Stimulation on Temporal Parameters of Human Sensorimotor Response and Their Relation to the Spectral Characteristics of the Initial EEG.

The effect of optical stimulation at a frequency of 10 Hz (OS) on temporal parameters of sensorimotor activity in healthy subjects (n=32) was studied. The expression of the activation response was determined by the ratio of spectral power values (SPα2, μV) of the high frequency (10-13 Hz) subrange of the α-rhythm of the initial EEG with closed and opened eyes and the frequency of the maximum α-peak (IAPF). A test for simple motor reaction time was performed under normal and OS conditions.

Changes in the Levels of Short-Chain Acids and Tryptophan Metabolites in Mouse Intestine during Experimental Colitis.

The optimal balance of the intestinal microbiota is considered to be an essential part of the human body that affects many metabolic processes. However, the exact role of the gut microbiota in metabolism is still not fully understood. To investigate the metabolic role of gut microbiota, the content of short-chain fatty acids and tryptophan metabolites was studied in mice with sodium dextran sulfate-induced colitis.

Expression of Somatostatin Receptors in the Small Intestine during Postnatal Ontogenesis.

The expression of somatostatin receptors (SSTRs) of types 1, 2, and 5 was studied in the small intestine of rats from different age groups (1, 10, 20, 30, 60 days, and 2-year-old) using Western blotting. The expression of SSTR1, SSTR2, and SSTR5 increased in the first 30 days of life, but decreased in older rats compared to 2-month-old animals. These findings suggest that there is differential expression of SSTRs during age-related development of the small intestine.

Consequences of Reprogrammed CD8 T-Cell Therapy for Lewis Lung Carcinoma Cells and Neovasculogenesis in C57BL/6 Mice.

We studied the effect of reprogrammed CD8 T cells (rT cells) from the bone marrow of intact mice on tumor cells and neovasculogenesis in mice with orthotopic Lewis lung carcinoma (LLC). Reprogramming of T cells was carried out using a MEK inhibitor and a PD-1 blocker; the targeting of rT cells to tumor cells was achieved by preincubation with LLC cell lysate. It was shown that the antitumor effect of rT cells was based on apoptosis of tumor cells.

Theoretical and Practical Aspects of the Nonlinear Dynamics' Methods of Heart Rate Variability Analyses in Tachyarrhythmia Patients Underwent Radiofrequency Catheter Ablation.

Purpose: This study explores the use of heart rate variability (HRV) analysis, a noninvasive technique for assessing the autonomic nervous system, by applying nonlinear dynamics and chaos theory to detect chaotic behavior in RR intervals and assess cardiovascular health.

Methods: Employing the "System Analysis of Heart Rate Dynamics" (SADR) program, this research combines chaos analysis with the short-time Fourier transform to assess nonlinear dynamic parameters in HRV. It includes constructing phase portraits in Takens space and calculating measures of chaos to identify deterministic chaos indicators.