Assessing cell lines with inducible depletion of cohesin and condensins components through analysis of metaphase chromosome morphology.

One of the most productive strategies for finding the functions of proteins is to study the consequences of loss of protein function. For this purpose, cells or organisms with a knockout of the gene encoding the protein of interest are obtained. However, many proteins perform important functions and cells or organisms could suddenly lose fitness when the function of a protein is lost.

Two new species of (Copepoda, Harpacticoida, Canthocamptidae) from the Russian Arctic and comparison with (Claus, 1863).

Two new species of Chappuis, 1929 from the Russian Arctic from the species group are described: and A complete morphological comparison of the new species with the type species (Claus, 1863) was carried out. Significant interspecific differences were shown at the level of microcharacters, such as integumental sensillae and pores, ornamentation of segments of mouthparts and swimming legs, and pores on swimming legs. A significant correlation has also been shown in the shape of the caudal rami of the females and the antennules of the males, which is likely caused by an evolutionary sexual arms race.

Males and ephippial females of Oriental Chydoridae (Cladocera: Anomopoda) from Cat Tien National Park, South Vietnam.

Cladocera (Crustacea: Branchiopoda) of South-East Asia have been intensively investigated over the last decades, but males and ephippial females of many taxa remain unknown or poorly studied. Males and ephippial females of ten species of Chydoridae were collected by monthly sampling of eight water bodies during autumn-winter dry season in Сat Tien National Park, South Vietnam. Morphology of studied specimens is described and taxonomic status of studied species is discussed.

Occurrence of the amphibians in the Volga, Don River basins and adjacent territories (Russia): research in 1996-2020.

Background: Knowledge about the distribution of living organisms on Earth is very important for many areas of biological science and understanding of the surrounding world. However, much of the existing distributional data are scattered throughout a multitude of sources, such as taxonomic publications, checklists and natural history collections and often, bringing them together is difficult. A very successful attempt to solve this problem is the GBIF project, which allows a huge number of researchers to publish data in one place in a single standard.

Re-evaluation of the genus Biapertura Smirnov, 1971 (Cladocera: Anomopoda: Chydoridae).

The genus Biapertura Smirnov, 1971, with type species B. affinis (= Lynceus affinis Leydig, 1860) is re-evaluated, removing the affinis-group from polyphyletic Alona s. lato.

"Flora of Russia" on iNaturalist: a dataset.

Background: The "Flora of Russia" project on iNaturalist brought together professional scientists and amateur naturalists from all over the country. Over 10,000 people were involved in the data collection.

New Information: Within 20 months, the participants accumulated 750,143 photo observations of 6,857 species of the Russian flora.

Priority of Carl Linnaeus as the author of the oldest species of Cladocera (Crustacea: Branchiopoda): Daphnia pulex (Linnaeus, 1758) and Polyphemus pediculus (Linnaeus, 1758).

In taxonomy, it is important to maintain chronological order in taxon naming and understanding. Here, I confirm that according to the rules of Zoological Nomenclature, Carl Linnaeus must be regarded as the author of two common cladoceran taxa, Daphnia pulex (Linnaeus, 1758) and Polyphemus pediculus (Linnaeus, 1758). Both were established in the 10th Edition of "Systema Naturae", the starting point of Zoological Nomenclature.

Slow-binding inhibition of acetylcholinesterase by an alkylammonium derivative of 6-methyluracil: mechanism and possible advantages for myasthenia gravis treatment.

Inhibition of human AChE (acetylcholinesterase) and BChE (butyrylcholinesterase) by an alkylammonium derivative of 6-methyluracil, C-547, a potential drug for the treatment of MG (myasthenia gravis) was studied. Kinetic analysis of AChE inhibition showed that C-547 is a slow-binding inhibitor of type B, i.e.

An annotated checklist of the Cladocera (Crustacea: Branchiopoda) of Colombia.

Based on the revision of available literature on the Colombian Cladocera (Crustacea: Branchiopoda), we present an annotated checklist, with taxonomical comments for all taxa recorded since the start of research on this group in the country in 1913. We have listed 101 valid taxa, of which most records belong to the Caribbean region of Colombia. The situation in Colombian Cladocera taxonomy is, at present, unfavorable for any realistic conclusions on biodiversity, ecology and biogeography.

Annotated Checklist of Chinese Cladocera (Crustacea: Branchiopoda). Part II. Order Anomopoda (families Macrotrichidae, Eurycercidae and Chydoridae).

About 114 "species" of Macrothrichidae, Eurycercidae, and Chydoridae (Cladocera, Anomopoda), belonging to 39 genera, have been reported from China, with 14 species in 6 genera in Macrothrichidae, 2 species in one genus in Eurycercidae, and 98 species in 31 genera in Chydoridae. In total, 203 species in 62 genera, 13 families and 4 orders have so far been reported from the country. Of these, 187 are tentatively considered as valid, while 16 are incertae sedis.

Association of polymorphisms near the FOXC2 gene with the risk of varicose veins in ethnic Russians.

Objective: To investigate the association of polymorphisms located near the FOXC2 gene with the risk of varicose veins in ethnic Russians.

Methods: Allele, genotype, and haplotype frequencies were determined in the sample of 474 patients with primary varicose veins and in the control group of 478 individuals without a history of chronic venous disease.

Results: Polymorphisms rs7189489, rs4633732, and rs1035550 showed the association with the increased risk of varicose veins, but none of the observed associations remained significant after correction for multiple testing.

Annotated Checklist of Chinese Cladocera (Crustacea: Branchiopoda). Part I. Haplopoda, Ctenopoda, Onychopoda and Anomopoda (families Daphniidae, Moinidae, Bosminidae, Ilyocryptidae).

Approximately 199 cladoceran species, 5 marine and 194 freshwater and continental saltwater species, live in China. Of these, 89 species are discussed in this paper. They belong to the 4 cladoceran orders, 10 families and 23 genera.

Exploring the limits for reduction of plastid genomes: a case study of the mycoheterotrophic orchids Epipogium aphyllum and Epipogium roseum.

The question on the patterns and limits of reduction of plastid genomes in nonphotosynthetic plants and the reasons of their conservation is one of the intriguing topics in plant genome evolution. Here, we report sequencing and analysis of plastid genome in nonphotosynthetic orchids Epipogium aphyllum and Epipogium roseum, which, with sizes of 31 and 19 kbp, respectively, represent the smallest plastid genomes characterized by now. Besides drastic reduction, which is expected, we found several unusual features of these "minimal" plastomes: Multiple rearrangements, highly biased nucleotide composition, and unprecedentedly high substitution rate.

Variations in O-antigen biosynthesis and O-acetylation associated with altered phage sensitivity in Escherichia coli 4s.

The O polysaccharide of the lipopolysaccharide (O antigen) of Gram-negative bacteria often serves as a receptor for bacteriophages that can make the phage dependent on a given O-antigen type, thus supporting the concept of the adaptive significance of the O-antigen variability in bacteria. The O-antigen layer also modulates interactions of many bacteriophages with their hosts, limiting the access of the viruses to other cell surface receptors. Here we report variations of O-antigen synthesis and structure in an environmental Escherichia coli isolate, 4s, obtained from horse feces, and its mutants selected for resistance to bacteriophage G7C, isolated from the same fecal sample.