Phage vB_KlebPS_265 Active Against Resistant/MDR and Hypermucoid K2 Strains of .

is an important opportunistic pathogen often resistant to antibiotics. Specific phages can be useful in eliminating infection caused by . phage vB_KlebPS_265 (KlebP_265) and its host strain were isolated from the sputum of a patient with infection.

Bacteriophage and Phage-Encoded Depolymerase Exhibit Antibacterial Activity Against K9-Type in Mouse Sepsis and Burn Skin Infection Models.

is a widely distributed nosocomial pathogen that causes various acute and chronic infections, particularly in immunocompromised patients. In this study, the activities of the K9-specific virulent phage AM24 and phage-encoded depolymerase DepAPK09 were assessed using in vivo mouse sepsis and burn skin infection models. In the mouse sepsis model, in the case of prevention or early treatment, a single K9-specific phage or recombinant depolymerase injection was able to protect 100% of the mice after parenteral infection with a lethal dose of of the K9-type, with complete eradication of the pathogen.

Development of Primer Panels for Whole-Genome Amplification and Sequencing of Human Seasonal Coronaviruses: hCoV-OC43, hCoV-HKU1, hCoV-229E, and hCoV-NL63.

Human seasonal coronaviruses (hCoVs) are a group of viruses that affect the upper respiratory tract. While seasonal patterns and the annual variability of predominant hCoV species are well-documented, their genetic and species diversity in St. Petersburg and across Russia remains largely unexplored.

Exploration of the Role of Cyclophilins in Established Hepatitis B and C Infections.

Cyclophilin (Cyp) inhibitors are of clinical interest in respect to their antiviral activities in the context of many viral infections including chronic hepatitis B and C. Cyps are a group of enzymes with peptidyl-prolyl isomerase activity (PPIase), known to be required for replication of diverse viruses including hepatitis B and C viruses (HBV and HCV). Amongst the Cyp family, the molecular mechanisms underlying the antiviral effects of CypA have been investigated in detail, but potential roles of other Cyps are less well studied in the context of viral hepatitis.

The Presence of Two Distinct Lineages of the Foot-And-Mouth Disease Virus Type A in Russia in 2013-2014 Has Significant Implications for the Epidemiology of the Virus in the Region.

Molecular surveillance of FMD epidemiology is a fundamental tool for advancing our understanding of virus biology, monitoring virus evolution, and guiding vaccine design. The accessibility of genetic data will facilitate a more comprehensive delineation of FMDV phylogeny on a global scale. In this study, we investigated the FMDV strains circulating in Russia during the 2013-2014 period in geographically distant regions utilizing whole genome sequencing followed by maximum-likelihood phylogenetic reconstruction of whole genome and VP1 gene sequences.

Isolation and Phylogenetic Analysis of Atypical Porcine Pestivirus Isolates Identified in Russian Swine Herds.

Atypical porcine pestivirus (APPV) was first identified in 2015 in North America by high-throughput sequencing. APPV is associated with congenital tremor A-II and is widely distributed worldwide. In this study, a total of 2630 samples of domestic pigs obtained from 14 regions of Russia from 2020 to 2024 were screened for APPV presence by qRT-PCR.

Nanotechnology-Based Drug Delivery Systems, 2nd Edition.

Nanotechnology is a promising and rapidly developing area in the 21st century, which affects various fields of science: physics, chemistry, biology, engineering, microelectronics, and medicine [...

Central Asia Cold Case: Siberian Pine Fingers New Suspects in Growth Decline CA 1700 CE.

Tree-ring width chronologies of Du Tour from near the upper treeline in the Western Sayan, Southern Siberia are found to have an exceptional (below mean-3SD) multi-year drop near 1700 CE, highlighted by the seven narrowest-ring years in a 1524-2022 regional chronology occurring in the short span of one decade. Tree rings are sometimes applied to reconstruct seasonal air temperatures; therefore, it is important to identify other factors that may have contributed to the growth suppression. The spatiotemporal scope of the "nosedive" in tree growth is investigated with a large network of (14 sites) and Ledeb.

Genome-Wide Association Study on Imputed Genotypes of 180 Eurasian Soybean Varieties for Oil and Protein Contents in Seeds.

Soybean () is a leguminous plant with a broad range of applications, particularly in agriculture and food production, where its seed composition-especially oil and protein content-is highly valued. Improving these traits is a primary focus of soybean breeding programs. In this study, we conducted a genome-wide association study (GWAS) to identify genetic loci linked to oil and protein content in seeds, using imputed genotype data for 180 Eurasian soybean varieties and the novel "genotypic twins" approach.

Brassinosteroids Render Cell Walls Softer but Less Extensible in Growing Arabidopsis Hypocotyls.

Cell wall extensibility is a key biophysical characteristic that defines the rate of plant cell growth. It depends on the wall structure and is controlled by numerous proteins that cut and/or (re)form links between the wall constituents. Cell wall extensibility is currently estimated by different in vitro biomechanical tests.

Fracture Toughness of Winding Carbon Plastics Based on Epoxy Matrices and Reinforced by Polysulfone Film.

In this work, the fracture mechanism of winding carbon-fiber-reinforced plastics (CFRPs) based on epoxy matrices reinforced by polysulfone film was investigated. Two types of polymer matrices were used: epoxy oligomer (EO) cured by iso-methyltetrahydrophthalic anhydride (iso-MTHPA), and EO-modified polysulfone (PSU) with active diluent furfuryl glycidyl ether (FGE) cured by iso-MTHPA. At the winding stage, the reinforcing film was placed in the middle layer of the CFRP.

Biomimetic Silicone Surfaces for Antibacterial Applications.

Biomimetic patterning emerges as a promising antibiotic-free approach to protect medical devices from bacterial adhesion and biofilm formation. The main advantage of this approach lies in its simplicity and scalability for industrial applications. In this study, we employ it to produce antibacterial coatings based on silicone materials, widely used in the healthcare industry.

Electrochemical Switching of Laser-Induced Graphene/Polymer Composites for Tunable Electronics.

Laser reduction of graphene oxide (GO) is a promising approach for achieving flexible, robust, and electrically conductive graphene/polymer composites. Resulting composite materials show significant technological potential for energy storage, sensing, and bioelectronics. However, in the case of insulating polymers, the properties of electrodes show severely limited performance.

Cost-Effective Production of Bacterial Cellulose and Tubular Materials by Cultivating B-11267 on a Molasses Medium.

An original design of a simple bioreactor was used to fabricate two tubular, 200 cm long BC structures by culturing B-11267 on a molasses medium. In addition, a tubular BC-based biocomposite with improved mechanical properties was obtained by combining cultivation on the molasses medium with in situ chemical modification by polyvinyl alcohol (PVA). Moreover, the present study investigated the BC production by the B-11267 strain on the media with different molasses concentrations under agitated culture conditions.

Sterically Induced Enhancement in the Electrochemical Stability of Salen-Type Cathode Materials.

This study investigates the electrochemical degradation mechanisms of nickel-salen (NiSalen) polymers, with a focus on improving the material's stability in supercapacitor applications. We analyzed the effects of steric hindrance near the nickel center by incorporating different bulky substituents into NiSalen complexes, aiming to mitigate water-induced degradation. Electrochemical performance was assessed using cyclic voltammetry, operando conductance, and impedance measurements, while X-ray photoelectron spectroscopy (XPS) provided insights into molecular degradation pathways.

Correction: Khavinson et al. Neuroprotective Effects of Tripeptides-Epigenetic Regulators in Mouse Model of Alzheimer's Disease. 2021, , 515.

In the original publication [...

Development of Ketoprofen Impurity A (1-(3-Benzoylphenyl)ethanone) as a Certified Reference Material for Pharmaceutical Quality Control.

: Reference materials are essential for ensuring the accuracy and traceability of measurements in the quality control of medicinal products. This study explores new principles for the preparation of impure materials of active pharmaceutical substances, focusing on 1-(3-benzoylphenyl)ethanone ketoprofen impurity A () as the reference material. : The reference material was synthesised from commercially available acetanilide and benzoyl chloride.

The Proviral Reservoirs of Human Immunodeficiency Virus (HIV) Infection.

Human Immunodeficiency Virus (HIV) proviral reservoirs are cells that harbor integrated HIV proviral DNA within their nuclear genomes. These cells form a heterogeneous group, represented by peripheral blood mononuclear cells (PBMCs), tissue-resident lymphoid and monocytic cells, and glial cells of the central nervous system. The importance of studying the properties of proviral reservoirs is connected with the inaccessibility of integrated HIV proviral DNA for modern anti-retroviral therapies (ARTs) that block virus reproduction.

Effect of the Current-Collecting Carbon Nanotubes Layer on the Properties of the Lead Zirconate Titanate Film for Vibration Sensors.

One of the challenging problems in the research and development of vibration sensors relates to the formation of Ohmic contacts for the removal of an electrical signal. In some cases, it is proposed to use arrays of carbon nanotubes (CNTs), which can serve as highly elastic electrode materials for vibration sensors. The purpose of this work is to study the effect of a current-collecting layer of CNTs grown over silicon on the properties of a lead zirconate titanate (PZT) film, which is frequently employed in mechanical vibration sensors or energy harvesters.

ZnO/MO Nanofiber Heterostructures: MO Receptor's Role in Gas Detection.

ZnO/MO (M = Fe, Co, Ni, Sn, In, Ga; [M]/([Zn] + [M]) = 15 mol%) nanofiber heterostructures were obtained by co-electrospinning and characterized by X-ray diffraction, scanning electron microscopy and X-ray fluorescence spectroscopy. The sensor properties of ZnO and ZnO/MO nanofibers were studied toward reducing gases CO (20 ppm), methanol (20 ppm), acetone (20 ppm), and oxidizing gas NO (1 ppm) in dry air. It was demonstrated that the temperature of the maximum sensor response of ZnO/MO nanofibers toward reducing gases is primarily influenced by the binding energy of chemisorbed oxygen with the surface of the modifier's oxides.

Modeling of Measuring Transducers for Relay Protection Systems of Electrical Installations.

The process of establishing relay protection and automation (RPA) settings for electric power systems (EPSs) entails complex calculations of operating modes. Traditionally, these calculations are based on symmetrical components, which require the building of equivalent circuits of various sequences. This approach can lead to errors both when identifying the operating modes and when modeling the RPA devices.

Assessment of Fluid Responsiveness via Central Venous Ultrasound Measurement: A Network Meta-Analysis.

Ultrasonographic assessment of the diameters of various veins and their indices are among the most applied diagnostic tools for evaluating fluid responsiveness in clinical practice. Despite their widespread use, there is no definitive answer on which is preferable. Our study aimed to investigate the diagnostic accuracy of different venous diameters and their indices to assess fluid responsiveness.

Spatial Distribution Dynamics of Sensory Disturbances in the Treatment of Obesity-Related Meralgia Paresthetica Using Transcutaneous Electrical Nerve Stimulation.

To date, there have been no studies on the dynamics of areas of pain, paraesthesia and hypoesthesia after the use of various transcutaneous electrical nerve stimulation in the treatment of meralgia paresthetica. In this pilot study, we observed 68 patients with obesity-related bilateral meralgia paresthetica. Pain syndrome, paraesthesia symptoms, and hypoesthesia were evaluated using 10-point scores.

Dynamics of Glycemic Status and Glucose Metabolism Markers 12 Months After Coronary Artery Bypass Grafting and Their Relationship with the Annual Prognosis of Patients.

We aim to evaluate the dynamics of glycemic status and markers of carbohydrate metabolism 12 months after coronary artery bypass grafting (CABG) and their relationship with the one-year prognosis. The analysis of outcomes of 653 patients during 1 year after coronary artery bypass grafting is presented. In those patients who visited the study center after 1 year, markers of carbohydrate metabolism (glucose, glycated hemoglobin, fructosamine, 1.

6-Bromoindole- and 6-Bromoindazole-Based Inhibitors of Bacterial Cystathionine γ-Lyase Containing 3-Aminothiophene-2-Carboxylate Moiety.

In recent years, a number of synthetic potentiators of antibiotics have been discovered. Their action can significantly enhance the antibacterial effect and limit the spread of antibiotic resistance through inhibition of bacterial cystathionine-γ-lyase. To expand the known set of potentiators, we developed methods for the synthesis of five new representatives of 6-bromoindole derivatives-potential inhibitors of bacterial cystathionine-γ-lyase-namely potassium 3-amino-5-((6-bromoindolyl)methyl)thiophene-2-carboxylate () and its 6-bromoindazole analogs ( and ), along with two 6-broindazole analogs of the parent compound .