Analysis of Cancer Stem Cell Markers in Various Histological Subtypes of Adrenocortical Cancer.

Adrenocortical cancer (ACC) is a rare malignant neoplasm originating from the adrenal cortex, presenting limited therapeutic options. An avenue for improving therapeutic efficacy may involve a deeper understanding of the role of adrenocortical stem/progenitor cells in the pathogenesis of this disease. Although existing data suggest stem/progenitor characteristics in certain cell populations within ACC, the challenge remains to identify adrenocortical stem cell markers directly involved in its carcinogenesis.

Nanopipettes as a Potential Diagnostic Tool for Selective Nanopore Detection of Biomolecules.

Nanopipettes, as a class of solid-state nanopores, have evolved into universal tools in biomedicine for the detection of biomarkers and different biological analytes. Nanopipette-based methods combine high sensitivity, selectivity, single-molecule resolution, and multifunctionality. The features have significantly expanded interest in their applications for the biomolecular detection, imaging, and molecular diagnostics of real samples.

Enhanced Analytical Performance in CYFRA 21-1 Detection Using Lateral Flow Assay with Magnetic Bioconjugates: Integration and Comparison of Magnetic and Optical Registration.

A novel approach to developing lateral flow assays (LFAs) for the detection of CYFRA 21-1 (cytokeratin 19 fragment, a molecular biomarker for epithelial-origin cancers) is proposed. Magnetic bioconjugates (MBCs) were employed in combination with advanced optical and magnetic tools to optimize assay conditions. The approach integrates such techniques as label-free spectral-phase interferometry, colorimetric detection, and ultrasensitive magnetometry using the magnetic particle quantification (MPQ) technique.

Ultrasensitive Lateral Flow Immunoassay of Fluoroquinolone Antibiotic Gatifloxacin Using Au@Ag Nanoparticles as a Signal-Enhancing Label.

Gatifloxacin (GAT), an antibiotic belonging to the fluoroquinolone (FQ) class, is a toxicant that may contaminate food products. In this study, a method of ultrasensitive immunochromatographic detection of GAT was developed for the first time. An indirect format of the lateral flow immunoassay (LFIA) was performed.

SERS Sensors with Bio-Derived Substrates Under the Way to Agricultural Monitoring of Pesticide Residues.

Uncontrolled use of pesticides in agriculture leads to negative consequences for the environment, as well as for human and animal health. Therefore, timely detection of pesticides will allow application of measures to eliminate the excess of maximum residue limits and reduce possible negative consequences in advance. Common methods of pesticide analysis suffer from high costs, and are time consuming, and labor intensive.

Correction: Mallphanov et al. Novel Approach to Increasing the Amplitude of the Mechanical Oscillations of Self-Oscillating Gels: Introduction of Catalysts Both as Pendant Groups and as Crosslinkers. 2024, , 727.

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Thermal Degradation Study of Hydrogel Nanocomposites Based on Polyacrylamide and Nanosilica Used for Conformance Control and Water Shutoff.

The application of nanocomposites based on polyacrylamide hydrogels as well as silica nanoparticles in various tasks related to the petroleum industry has been rapidly developing in the last 10-15 years. Analysis of the literature has shown that the introduction of nanoparticles into hydrogels significantly increases their structural and mechanical characteristics and improves their thermal stability. Nanocomposites based on hydrogels are used in different technological processes of oil production: for conformance control, water shutoff in production wells, and well killing with loss circulation control.

Processes of Obtaining Nanostructured Materialswith a Hierarchical Porous Structure on the Example of Alginate Aerogels.

Currently, materials with specific, strictly defined functional properties are becoming increasingly important. A promising strategy for achieving these properties involves developing methods that facilitate the formation of hierarchical porous materials that combine micro-, meso-, and macropores in their structure. Macropores facilitate effective mass transfer of substances to the meso- and micropores, where further adsorption or reaction processes can occur.

Antimicrobial Zn-Carboxymethyl Chitosan Cryogel for Controlled Loading and Release of Ciprofloxacin via Coordination Bonds.

The local application of broad-spectrum antibiotics via polymeric drug delivery systems is a promising alternative to their systemic administration in wound healing, prevention and treatment of infections associated with surgical implants. However, low and poorly controlled loading efficiency and 100% burst release are common problems for the materials with weak physical interaction between antibiotics and polymeric matrices. Here, we report a new multifunctional carboxymethyl chitosan (CMC) cryogel, which efficiently prevents bacterial adhesion to the surface, kills bacteria in the solution via controlled release of ciprofloxacin (CIP), and promotes fibroblast proliferation.

L-Cysteine/Silver Nitrate/Iodate Anions System: Peculiarities of Supramolecular Gel Formation with and Without Visible-Light Exposure.

In this study, novel anion photo-responsive supramolecular hydrogels based on cysteine-silver sol (CSS) and iodate anions (IO) were prepared. The peculiarities of the self-assembly process of gel formation in the dark and under visible-light exposure were studied using a complex of modern physico-chemical methods of analysis, including viscosimetry, UV spectroscopy, dynamic light scattering, electrophoretic light scattering, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray photoelectron spectroscopy. In the dark phase, the formation of weak snot-like gels takes place in a quite narrow IO ion concentration range.

Composite Contrast Enhancement of Hydrogel-Based Implants for Photon-Counting Computed Tomography Studies.

Hydrogels have a wide range of medical applications, including use within implantable systems. However, when used in implants, their visibility under conventional medical imaging techniques is limited, creating safety risks for patients. In the current work, we assessed the possibility of enhancing hydrogels using Ln-based contrasting agents to facilitate their visualization in photon-counting computed tomography (PCCT).

The Use of AgNP-Containing Nanocomposites Based on Galactomannan and κ-Carrageenan for the Creation of Hydrogels with Antiradical Activity.

Series of composites containing 2.5-17.0% Ag and consisting of spherical silver nanoparticles with sizes ranging from 5.

Enhancement of Water Uptake in Composite Superabsorbents Based on Carboxymethyl Cellulose Through Porogen Incorporation and Lyophilization.

Carboxymethyl cellulose sodium salt (CMC)-based superabsorbents are promising materials for the development of agricultural matrices aimed at water management and slow-release fertilizer production. However, an increase in the CMC content tends to reduce their water-absorbing capacity. This study aims to develop a cost-effective method for producing eco-friendly superabsorbents with enhanced water-absorbing capacity by incorporating a porogen and employing lyophilization.

Hemostatic Antimicrobial Hydrogels Based on Silicon, Iron, Zinc, and Boron Glycerolates for Wound Healing Applications.

The use of glycerolates of biogenic elements as biocompatible precursors in sol-gel synthesis is an innovative direction and opens up new scientific and practical prospects in chemistry and technology of producing practically important biomedical materials, including hemostatic, antimicrobial, and wound healing materials. Using biocompatible precursors, silicon, zinc, boron, and iron glycerolates, new bioactive nanocomposite hydrogels were obtained by the sol-gel method. The composition and structural features of the hydrogels were studied using a complex of modern analytical techniques, including TEM, XRD, AES, and ESI MS.

Synthesis and Application of SAPO-11 Molecular Sieves Prepared from Reaction Gels with Various Templates in the Hydroisomerization of Hexadecane.

Among the most selective catalytic systems for the hydroisomerization of C-paraffins, catalytic systems based on SAPO-11 are quite promising. In order to increase the activity and selectivity of these bifunctional catalysts, it is necessary to reduce the diffusion restrictions for the reacting molecules and their products in the microporous structure of SAPO-11 by reducing the crystal size. To solve this problem, we have studied the influence of different templates (diethylamine, dipropylamine, diisopropylamine, and dibutylamine) on the physicochemical properties of reaction gels and SAPO-11 silicoaluminophosphates during their crystallization.

Self-Assembled Hydrogel Based on (Bio)polyelectrolyte Complex of Chitosan-Gelatin: Effect of Composition on Physicochemical Properties.

Taking into account the trends in the field of green chemistry and the desire to use natural materials in biomedical applications, (bio)polyelectrolyte complexes ((bio)PECs) based on a mixture of chitosan and gelatin seem to be relevant systems. Using the approach of self-assembly from the dispersion of the coacervate phase of a (bio)PEC at different ratios of ionized functional groups of chitosan and gelatin (), hydrogels with increased resistance to mechanical deformations and resorption in liquid media were obtained in this work in comparison to a hydrogel from gelatin. It was found that at ≥ 1 a four-fold increase in the elastic modulus of the hydrogel occurred in comparison to a hydrogel based on gelatin.

Nanostructured Hydrogels of Carboxylated Cellulose Nanocrystals Crosslinked by Calcium Ions.

Bio-based eco-friendly cellulose nanocrystals (CNCs) gain an increasing interest for diverse applications. We report the results of an investigation of hydrogels spontaneously formed by the self-assembly of carboxylated CNCs in the presence of CaCl using several complementary techniques: rheometry, isothermal titration calorimetry, FTIR-spectroscopy, cryo-electron microscopy, cryo-electron tomography, and polarized optical microscopy. Increasing CaCl concentration was shown to induce a strong increase in the storage modulus of CNC hydrogels accompanied by the growth of CNC aggregates included in the network.

Effect of Carrot Callus Cells on the Mechanical, Rheological, and Sensory Properties of Hydrogels Based on Xanthan and Konjac Gums.

The study aims to develop a plant-based food gel with a unique texture using callus cells and a mixture of xanthan (X) and konjac (K) gums. The effect of encapsulation of carrot callus cells (0.1 and 0.

The Effects of the Combined Co-Expression of GroEL/ES and Trigger Factor Chaperones on Orthopoxvirus Phospholipase F13 Production in .

Heterologous protein expression often faces significant challenges, particularly when the target protein has posttranslational modifications, is toxic, or is prone to misfolding. These issues can result in low expression levels, aggregation, or even cell death. Such problems are exemplified by the expression of phospholipase p37, a critical target for chemotherapeutic drugs against pathogenic human orthopoxviruses, including monkeypox and smallpox viruses.

Composition, Seasonal Dynamics and Metabolic Potential of the Rhizosphere Microbiome Associated with Wild White Poplar.

The white poplar () is a dioecious woody plant with significant potential for the phytoremediation of soils. To realize this potential, it is necessary to utilize growth-promoting microorganisms. One potential source of such beneficial microorganisms is the rhizosphere community of wild-growing trees.

Some Human Anti-Glycan Antibodies Lack the Ability to Activate the Complement System.

Naturally occurring human antibodies against glycans recognize and quickly eliminate infectious bacteria, viruses and aberrantly glycosylated neoplastic malignant cells, and they often initiate processes that involve the complement system. Using a printed glycan array (PGA) containing 605 glycoligands (oligo- and polysaccharides, glycopeptides), we examined which of the glycan-binding antibodies are able to activate the complement system. Using this PGA, the specificities of antibodies of the IgM and IgG classes were determined in the blood serum of healthy donors (suggested as mostly natural), and, then, using the same array, it was determined which types of the bound immunoglobulins were also showing C3 deposition.

The Efficacy of Er:YAG Laser in the Extraction of Impacted Third Molars: A Randomized Clinical Trial.

: Impacted third molar extraction with a scalpel and rotary instruments is one of the most traumatic surgeries in dentistry. Therefore, it is necessary to discover less traumatic methods and instruments to reduce the risk of postoperative complications. : This study is reported in accordance with the CONSORT guidelines.

The Maturation of the International Health Crisis Response: The Polish Typhus Epidemic of 1916-1923 Compared to the African Ebola Virus Disease Epidemic of 2013-2016: Part I, the Polish Epidemic.

Poland suffered an epidemic of louse-borne typhus from 1916-1923, with 400,000 cases and more than 130,000 deaths. The causative factors were depressed economic conditions and a refugee crisis that engulfed Poland after World War I. The recognition of the epidemic in 1919 stimulated the creation of the League of Red Cross Societies (LRCS).

Cappable-seq RNA-sequencing data sets of K-12 MG1655 treated with novobiocin, tetracycline, and rifampicin.

Mapping transcription start sites and determining their activity remain a challenging task even for well-studied organisms. Here, we present Cappable-seq RNA sequencing data of K-12 MG1655 after treatment with three antibiotics with various spectra of action that may expand the range of mapped transcription start sites for this model organism.

Draft genome sequences of strain I-540 isolated from grape must.

is a non-Saccharomyces yeast that is widely used in winemaking due to its ability to ferment malic acid, thus improving organoleptic properties of wine. We report the draft genome sequence of strain I-540, isolated from grape must in Russia.