Case report: Relapse of intrathyroidal parathyroid carcinoma in a patient with novel variants in and genes.

Parathyroid carcinoma (PC) is one of the rarest malignant neoplasms of the human endocrine system, with a prevalence of approximately 0.005% of all oncological diseases. Despite its indolent course, PC generally relapses in about 40%-60% of cases.

Predicting patient outcomes with gene-expression biomarkers from colorectal cancer organoids and cell lines.

Introduction: Colorectal cancer (CRC) is characterized by an extremely high mortality rate, mainly caused by the high metastatic potential of this type of cancer. To date, chemotherapy remains the backbone of the treatment of metastatic colorectal cancer. Three main chemotherapeutic drugs used for the treatment of metastatic colorectal cancer are 5-fluorouracil, oxaliplatin and irinotecan which is metabolized to an active compound SN-38.

First record of Serban, 2000 (Bathynellacea, Bathynellidae) from subterranean waters of South Korea, with the description of a new species.

This study describes , the first new species of Bathynellidae family reported in East Asia since 2000, and it presents the first molecular analysis using CO1 and 18S gene sequences. Morphological analysis reveals that the new species and previously known species uniquely share key characteristics in the male and female thoracopods VIII. However, the presence or absence of the median seta on the antenna exopod, along with the length differences between the four spines in the furca, distinguish the new species from , 2000.

Effect of Gadolinium Doping on the Optical and Magnetic Properties of Red-Emitting Dual-Mode Carbon Dot-Based Probes for Magnetic Resonance Imaging.

Bioimaging probes based on carbon dots (CDs) can become a useful replacement for existing commercial probes, benefiting clinical diagnostics. While the development of dual-mode CD-based probes for magnetic resonance imaging (MRI), which provides the ability for photoluminescence (PL) detection at the same time, is ongoing, several challenges have to be addressed. First, most of the CD-based probes still emit at shorter wavelengths (blue/green spectral range), which is harmful to biological objects or have very low PL intensity in the biological window of tissue transparency (red/near-infrared spectral range).

-mutated AML: Immune Evasion Through Exosome-Mediated Mechanisms and innovative combination therapies targeting Immune escape.

Introduction: Acute Myeloid Leukemia is a heterogeneous hematological malignancy characterized by the uncontrolled proliferation of abnormal myeloid cells. Besides several other genetic abnormalities developed in AML, FLT3 mutations are significant due to their worse prognostic impacts and therapeutic resistance. As a result, these mutations enable AML cells to develop mechanisms for evading immune surveillance.

The crosstalk between SND1 and PDCD4 is associated with chemoresistance of non-small cell lung carcinoma cells.

Lung cancer is the leading cause of cancer-related deaths worldwide. Non-small cell lung cancer (NSCLC) is highly resistant to chemo- or radiation therapy, which poses a huge challenge for treatment of advanced NSCLC. Previously, we demonstrated the oncogenic role of Tudor Staphylococcal nuclease (TSN, also known as Staphylococcal nuclease domain-containing protein 1, SND1), in regulating chemoresistance in NSCLC cells.

Assessing sub-Saharan Africa's GHG emissions from croplands: environmental impacts and sustainable mitigation strategies.

Globally, agricultural lands are among the top emitters of greenhouse gases (GHGs), responsible for over 20% of total greenhouse gas (GHG) emissions. Climatic conditions, an acute challenge in sub-Saharan Africa (SSA), where access to mitigation technologies remains limited, have heavily influenced these lands. This study explores GHG contributions from crop production and their devastating and deteriorating impacts on the economy and environment and proposes a sustainable solution.

Trends and challenges in bispecific antibody production.

Bispecific antibodies (bsAbs) represent a rapidly growing field of therapeutic agents. More bsAbs are being approved worldwide and are in various stages of clinical trials. However, the discovery and production of novel bsAbs presents significant challenges due to their complex structure.

Prospective trial of the Oncobox platform RNA sequencing bioinformatic analysis for personalized prescription of targeted drugs.

Interrogating gene expression in tumor can identify up- and downregulated molecular targets of cancer drugs. Here we report the results of prospective clinical investigation of using RNA sequencing analysis for personalized cancer therapy. Transcriptomic profiles were analyzed using Oncobox platform that identifies altered expression of drug target genes and molecular pathways and builds a personalized rating of targeted therapeutics.

Ancient genomics and the origin, dispersal, and development of domestic sheep.

The origins and prehistory of domestic sheep () are incompletely understood; to address this, we generated data from 118 ancient genomes spanning 12,000 years sampled from across Eurasia. Genomes from Central Türkiye ~8000 BCE are genetically proximal to the domestic origins of sheep but do not fully explain the ancestry of later populations, suggesting a mosaic of wild ancestries. Genomic signatures indicate selection by ancient herders for pigmentation patterns, hornedness, and growth rate.

Prevalence and genetic diversity of tick-borne encephalitis virus in ixodid ticks from specific regions of northwestern Russia.

Russia is a country with a high incidence of tick-borne encephalitis (TBE). In northwestern regions of Russia, 110 TBE cases were registered in 2021. The largest numbers of TBE cases were registered in the Arkhangelsk region and St.

Rejection of the concept of hemiurid genus Pulmovermis (Digenea: Hemiuridae) and other taxonomic propositions: new morphological and molecular data regarding Lecithochirium cyanovitellosum (Coil and Kuntz, 1960) Urabe and Sokolov, comb. nov. (formerly Pulmovermis cyanovitellosus).

Pulmovermis cyanovitellosus Coil and Kuntz, 1960 is a species of hemiurid trematode that localizes in the lung of sea snakes, an unusual trait for this group of parasites. Recent molecular phylogenetic studies based on 28S rRNA gene sequences have shown that this species is closely related to members of the genus Lecithochirium Lühe, 1901. This finding is unexpected given that Pulmovermis Coil and Kuntz, 1960 and Lecithochirium are currently classified in different subfamilies of Hemiuridae (Pulmoverminae Sandars, 1961 vs.

Why has the 'miracidium' of Notocotylidae (Trematoda: Digenea) lost all stage-specific traits?

Digeneans of the family Notocotylidae differ from other digeneans in their peculiar eggs. The eggs feature a pair of long filaments extending from their poles, and their contents differ significantly from what we expect to observe in the eggs of digeneans. Instead of a ciliated miracidium larva, the notocotylid egg contains a tiny few-celled mother sporocyst.

History and prospects of flax genetic markers.

Flax ( L.) is known as a dual-purpose crop, producing both fiber and oil, which have a wide range of uses. Successful flax breeding requires knowledge on the genetic determinants of flax traits.

does not give an advantage to the ectoparasitoid (Say, 1836) when it develops on an infected host.

The effect of on the viability and antimicrobial activity of the ectoparasitoid was evaluated in laboratory experiments. Two lines of the parasitoid, -infected (W+) and -free (W-), were used. Parasitoid larvae were fed with a host orally infected with a sublethal dose of (Bt) and on the host uninfected with Bt.

Evaluation of Lyso-Gb1 as a biomarker for Gaucher disease treatment outcomes using data from the Gaucher Outcome Survey.

Background: Patients with Gaucher disease (GD) require continual monitoring; however, lack of specific disease biomarkers was a significant challenge in the past. Glucosylsphingosine (lyso-Gb1) has been shown to be a reliable, key, specific, and sensitive biomarker for diagnosis, prognosis, and treatment response in clinical studies of patients with GD. We evaluated the change in lyso-Gb1 concentration over time following enzyme replacement therapy in patients with confirmed GD using real-world data from the Gaucher Outcome Survey disease registry.

Polyp and tumor microenvironment reprogramming in colorectal cancer: insights from mucosal bacteriome and metabolite crosstalk.

Background: Highly frequent colorectal cancer (CRC) is predicted to have 3.2 million novel cases by 2040. Tumor microenvironment (TME) bacteriome and metabolites are proposed to be involved in CRC development.

Sanger validation of WGS variants.

With the development of next-generation sequencing (NGS) technologies it became possible to simultaneously analyze millions of variants. Despite the quality improvement, it is generally still required to confirm the variants before reporting. However, in recent years the dominant idea is that one could define the quality thresholds for "high quality" variants which do not require orthogonal validation.

Salivary steroids in acute central serous chorioretinopathy.

Purpose: To analyze levels of salivary steroids, including 17-OH-progesterone (17-OHP), androstenedione, dehydroepiandrosterone, cortisol, cortisone, progesterone, testosterone, and estradiol, in patients with acute central serous chorioretinopathy (CSCR) patients.

Methods: Acute CSCR patients and healthy individuals were included in this observational case-control study. Levels of salivary steroids were determined by high-performance liquid chromatography with tandem mass spectrometry detection.

State-of-the-Art Personalized Therapy Approaches for Chronic Non-Specific Low Back Pain: Understanding the Mechanisms and Drivers.

Chronic non-specific low back pain (CNSLBP) is a debilitating condition that affects millions of people worldwide, significantly impacting quality of life and imposing a substantial socioeconomic burden. Traditional treatment approaches often rely on a one-size-fits-all strategy, failing to account for individual variations in pathophysiological mechanisms, drivers, and the principles of personalized medicine. Furthermore, an overemphasis on biomechanical findings from imaging may lead to ineffective interventions and unnecessary surgical procedures, obscuring other important factors that contribute to pain perception.

Cobalt polygalacturonates and the pharmacological composition based on them: Preparation, properties and cytotoxicity.

The aim of the present study was to obtain new metal complexes of citrus pectin with cobalt ions based on potassium polygalacturonate and to prepare a new pharmacological composition (PC) PGKCo: PGNaCo (1:1) with antitumor activity based on potassium cobalt polygalacturonate (PGKCo) and sodium cobalt polygalacturonate (PGNaCo). The study of the effect of PGKCo, PGNaCo and PC on the cell viability of tumor cell lines of different genesis in vitro showed that the obtained compounds are soluble in water and exhibit selective cytotoxic activity against the tumor cell lines of human lung carcinoma A549, breast adenocarcinoma MCF-7 and cervical carcinoma M-HeLa, with no significant toxic effect on normal human cells. The possible mechanism of action of the investigated PC on M-HeLa cancer cells was investigated.

Metabolic profiling of Achillea millefolium from the Chernobyl exclusion zone reveals the adaptive strategies to low-dose chronic radiation exposure.

The radionuclide contamination of the environment is an abiotic stress factor that influences biological systems. Plants growing in contaminated areas for many generations provide a unique opportunity to study adaptive strategies aimed at maintaining homeostasis under elevated radiation levels. Using non-targeted metabolomics approaches, we investigated the metabolomic profiles of Achillea millefolium L.

Prolgolimab with chemotherapy as first-line treatment for advanced non-squamous non-small-cell lung cancer.

Background: Prolgolimab is an IgG1 anti-PD-1 monoclonal antibody with the Fc-silencing 'LALA' mutation. The phase III DOMAJOR study assessed efficacy and safety of prolgolimab in combination with pemetrexed and platinum-based chemotherapy vs placebo in combination with pemetrexed and platinum-based chemotherapy as first-line treatment for advanced non-small cell lung cancer (NSCLC).

Methods: 292 patients with advanced non-squamous NSCLC were randomized 1:1 to receive 4 cycles of pemetrexed, platinum-based drug and either prolgolimab (3 mg/kg Q3W) or placebo followed by prolgolimab/placebo with pemetrexed until disease progression or toxicity (≤36 months).

Chromosome length genome assembly of the stone marten (Martes foina, Mustelidae): a new view on one of the cornerstones in carnivore cytogenetics.

The stone marten (Martes foina) is an important species for cytogenetic studies in the order Carnivora. ZooFISH probes created from its chromosomes provided a strong and clean signal in chromosome painting experiments and were valuable for studying the evolution of carnivoran genome architecture. The research revealed that the stone marten chromosome set is similar to the presumed ancestral karyotype of the Carnivora, which added an additional value for the species.