Identification of a new alphacoronavirus (Coronaviridae: ) associated with the greater horseshoe bat () in the south of European part of Russia.

Introduction: Bats are recognized as primary natural reservoirs for alpha- and betacoronaviruses. The interspecies transmission of bat coronaviruses to other mammalian hosts, including livestock and humans, can lead to epidemics, epizootics, and global pandemics.

Objective: This study aims to describe coronaviruses associated with horseshoe bats ( spp.

[Prevalence and phylogenetic analysis of cytomegalovirus (Orthoherpesviridae: ) genetic variants from children and immunocompromised patients in central Russia].

Introduction: Cytomegalovirus (CMV) is a DNA-containing virus that is widespread worldwide and is of great importance in infectious pathology of children and adults. The aim of this study is to evaluate the prevalence of CMV among children and immunocompromised patients in the Nizhny Novgorod region (central Russia) and to perform a phylogenetic analysis of the identified strains.

Materials And Methods: DNA samples of CMV detected in frequently ill children and adult recipients of solid organs were studied.

The features of Tat protein of human immunodeficiency virus type 1 (Retroviridae: ) non-A6 variants, characteristic for the Russian Federation.

Introduction: Tat protein is a trans-activator of HIV-1 genome transcription, with additional functions including the ability to induce the chronic inflammatory process. Natural amino acid polymorphisms in Tat may affect its functional properties and the course of HIV infection. The aim of this work is to analyze the marks of Tat consensus sequences in non-A6 HIV-1 variants characteristic of the Russian Federation, as well as study natural polymorphisms in Tat CRF63_02A6 and subtype B variants circulating in Russia.

[Analysis of changes in the genome of the Omsk hemorrhagic fever virus (Flaviviridae: ) during laboratory practices for virus preservation].

Introduction: Omsk hemorrhagic fever (OHF) is a severe disease identified in the 1940s in Western Siberia, Russia. Disease is caused by the OHF virus, which belongs to the genus . The purpose of the work.

Retrospective analysis and forecasting of the spread of viruses in real time: the case of COVID-19 in St. Petersburg and Moscow in 2020-2021.

The aim of the study is to apply mathematical methods to generate forecasts of the dynamics of random values of the percentage increase in the total number of infected people and the percentage increase in the total number of recovered and deceased patients. The obtained forecasts are used for retrospective forecasting of COVID-19 epidemic process dynamics in St. Petersburg and in Moscow.

Ravulizumab demonstrates long-term efficacy, safety and favorable patient survival in patients with paroxysmal nocturnal hemoglobinuria.

Ravulizumab is a second-generation complement component 5 (C5) inhibitor (C5i) approved for the treatment of paroxysmal nocturnal hemoglobinuria (PNH) following positive results from two pivotal trials in patients with PNH originally naive to C5i treatment and eculizumab-experienced patients with PNH. In both trials, after the 26week primary evaluation period, all patients received ravulizumab for up to 6 years. To report ravulizumab treatment outcomes in patients with PNH originally naive to C5i treatment and eculizumab-experienced patients with PNH treated for up to 6 years.

Bottlebrush Pastes as a Platform for Solvent-Free, Injectable, and Shape-Persistent Materials with Tissue-Mimetic Viscoelasticity.

Architecturally hindered crystallization of bottlebrush graft copolymers offers a reaction- and solvent-free pathway for creating injectable elastomers with tissue-mimetic softness. Currently, injectable materials involve solvents and chemical reactions, leading to uncontrolled swelling, leaching of unreacted moieties, and side reactions with tissue. To address this issue, bottlebrush copolymers with a poly(ethylene glycol) (PEG) amorphous block and crystallizable poly(lactic acid) (PLA) grafted chains (A--B) were synthesized, with grafted chains of controlled length arranged along the backbone at controlled spacing.

Digestive System Development and Posterior Hox/Parahox Gene Expression During Larval Life and Metamorphosis of the Phoronid Phoronopsis harmeri.

Phoronida is a small group of marine animals, most of which are characterized by a long larval period and complex metamorphosis. As a result of metamorphosis, their body changes so much that their true anterior and posterior ends are very close to each other, and the intestine becomes long and U-shaped. Using histology and electron microscopy, we have shown that the elongation and change in shape of the digestive tract that occurs during metamorphosis in Phoronopsis harmeri larvae is accompanied by the formation of new parts and changes in ultrastructure.

Detection of clinically relevant variants in the gene below 10% allelic frequency: A multicenter study by ERIC, the European Research Initiative on CLL.

In chronic lymphocytic leukemia, the reliability of next-generation sequencing (NGS) to detect variants ≤10% allelic frequency (low-VAF) is debated. We tested the ability to detect 23 such variants in 41 different laboratories using their NGS method of choice. The sensitivity was 85.

The neurosociological paradigm of the metaverse.

Metaverse integrates people into the virtual world, and challenges depend on advances in human, technological, and procedural dimensions. Until now, solutions to these challenges have not involved extensive neurosociological research. The study explores the pioneering neurosociological paradigm in metaverse, emphasizing its potential to revolutionize our understanding of social interactions through advanced methodologies such as hyperscanning and interbrain synchrony.

Detection of cervical precancerous lesions and cancer by small-scale RT-qPCR analysis of oppositely deregulated mRNAs pairs in cytological smears.

Background: Cervical screening, aimed at detecting precancerous lesions and preventing cancer, is based on cytology and HPV testing. Both methods have limitations, the main ones being the variable diagnostic sensitivity of cytology and the moderate specificity of HPV testing. Various molecular biomarkers are proposed in recent years to improve cervical cancer management, including a number of mRNAs encoded by human genes involved in carcinogenesis.

Comparative evaluation of ACetic - MEthanol high salt dissociation approach for single-cell transcriptomics of frozen human tissues.

Current dissociation methods for solid tissues in scRNA-seq studies do not guarantee intact single-cell isolation, especially for sensitive and complex human endocrine tissues. Most studies rely on enzymatic dissociation of fresh samples or nuclei isolation from frozen samples. Dissociating whole intact cells from fresh-frozen samples, commonly collected by biobanks, remains a challenge.

Effect of diet low in omega-6 polyunsaturated fatty acids on the global burden of cardiovascular diseases and future trends: evidence from the Global Burden of Disease 2021.

Background: This research analyzes the worldwide impact of cardiovascular diseases (CVD) associated with low consumption of omega-6 polyunsaturated fatty acids, utilizing data from the 2021 Global Burden of Disease Study.

Method: The study explored the influence of diets deficient in omega-6 polyunsaturated fatty acids on CVD across global, regional, and national levels. It examined variations across different age groups and genders and analyzed the relationship between the disease burden and the socio-demographic index (SDI).

Deep learning captures the effect of epistasis in multifactorial diseases.

Background: Polygenic risk score (PRS) prediction is widely used to assess the risk of diagnosis and progression of many diseases. Routinely, the weights of individual SNPs are estimated by the linear regression model that assumes independent and linear contribution of each SNP to the phenotype. However, for complex multifactorial diseases such as Alzheimer's disease, diabetes, cardiovascular disease, cancer, and others, association between individual SNPs and disease could be non-linear due to epistatic interactions.

Evaluation of diesel engine performance and emissions using biodiesel from waste oils synthesized with FeO-SiO heterogeneous nano catalyst.

Purpose: Biodiesel is a non-toxic, renewable, and environmentally friendly fuel used in compression ignition engines. This work aimed to develop FeO/SiO as a cheap, magnetic, and easy separable catalyst for biodiesel production from waste oil by sono-catalytic transesterification.

Methods: Fe₃O₄-SiO₂ was prepared using a modified Stober method and used as a heterogeneous catalyst in an ultrasound-assisted transesterification reaction to produce biodiesel.

Age-dependent phenotypes of cognitive impairment as sequelae of SARS-CoV-2 infection.

Cognitive changes associated with PASC may not be uniform across populations. We conducted individual-level pooled analyses and meta-analyses of cognitive assessments from eight prospective cohorts, comprising 2,105 patients and 1,432 controls from Argentina, Canada, Chile, Greece, India, Italy, Russia, and the UK. The meta-analysis found no differences by country of origin.

Geographical distribution and evolutionary dynamics of H4Nx avian influenza viruses.

H4Nx avian influenza viruses (AIVs) have been isolated from wild birds and poultry and can also cross the species barrier to infect mammals (pigs and muskrats). The widespread presence of these viruses in wild birds and poultry and their ability to be transmitted interspecies make them an undeniable hazard to the poultry farming industry. In the present study, we collected fecal and swab samples from wild birds and poultry in Guangdong Province from January 2019 to March 2024, and various subtypes of AIVs were isolated, including 19 strains of H4 subtype AIVs.

The freeze-thaw cycle effect on blood serum autofluorescence, Raman spectroscopy and SERS: implications for sample classification and disease diagnostics.

The issue of variability introduced into blood plasma and serum analysis by preanalytical procedures is the major obstacle to obtaining accurate and reproducible results. While the question of how to overcome this issue has been discussed in biochemical detection of analytes and omics technologies, its relevance to the field of optical spectroscopy remains mostly unexplored. In this work, we evaluated the freeze-thaw cycle (FTC)-induced alternations in blood serum optical properties by means of autofluorescence and Raman spectroscopy, including surface-enhanced Raman spectroscopy (SERS).

Bacteria Flagella-Mimicking Polymer Multilayer Magnetic Microrobots.

Mass production of biomedical microrobots demands expensive and complex preparation techniques and versatile biocompatible materials. Learning from natural bacteria flagella, the study demonstrates a magnetic polymer multilayer cylindrical microrobot that bestows the controllable propulsion upon an external rotating magnetic field with uniform intensity. The magnetic microrobots are constructed by template-assisted layer-by-layer technique and subsequent functionalization of magnetic particles onto the large opening of the microrobots.

The Expression of the LDLR, LDLRAP1, and PCSK9 Genes has Prognostic Significance in Triple-negative Breast Cancer.

Aims: The purpose of this study was to investigate the prognostic significance of cholesterol uptake genes in predicting the survival of breast cancer patients.

Background: Cholesterol plays a crucial role in the homeostasis of tumor cells. It is known that cholesterol levels can influence important parameters of the disease, such as sensitivity to therapy, progression, and metastasis of cancer.

Nanoparticles as Delivery Vehicles for Vaccines: The Use of Gold Nanoparticles.

Since their inception, therapeutic or prophylactic vaccines have emerged as promising candidates for the prevention or treatment of infections and various diseases, including cancer and autoimmune disorders. In recent times, gold nanoparticles (GNPs) have acquired active roles in the field of vaccine development due to their intrinsic capacity to adjust and enhance the immune response. Due to their characteristics, GNPs can exert optimal effects as both delivery vehicles and adjuvants.