Development and validation of 2 probe-hybridization quantitative PCR assays for rapid detection of a pathogenic species in captive psittacines.

Avian coxiellosis is an emerging cause of morbidity and mortality among captive psittacines, and the utility of a rapid detection test using easily obtained samples is paramount in a clinical setting. New sequences were obtained from 3 genes: , and . We developed probe-hybridization quantitative PCR (qPCR) assays using and genes.

Physiologic responses and plasma endothelin-1 concentrations associated with abrupt cessation of nitric oxide inhalation in isoflurane-anesthetized horses.

Objective: To assess physiologic responses and plasma endothelin (ET)-1 concentrations associated with abrupt cessation of nitric oxide (NO) inhalation in isoflurane-anesthetized horses.

Animals: 6 healthy adult Standardbreds.

Procedures: Horses were anesthetized with isoflurane in oxygen and placed in dorsal recumbency.

Assessment of serum concentrations and sedative effects of fentanyl after transdermal administration at three dosages in healthy llamas.

Objective: To determine the serum concentrations and sedative effects of fentanyl after transdermal administration at 3 dosages in llamas.

Animals: 9 healthy adult female llamas (mean age, 8 +/- 3 years; mean weight, 150 +/- 18 kg).

Procedure: Llamas were allocated to 1 of 3 groups (3 llamas/group).