Microbiome profiling suggests novel endosymbiont associations of insect pests of stored grain.

Many arthropods, including economically important pests of stored grains, host intracellular bacterial symbionts. These symbionts can have diverse impacts on host morphology, stress tolerance, and reproductive success. The ability to rapidly determine the infection status of host insects and the identity of intracellular symbionts, if present, is vital to understanding the biology and ecology of these organisms.

An optimized spectral reconstruction method for shift excitation Raman differential spectroscopy.

Raman spectroscopy is a powerful analytical method, but when the composition of the test sample is intricate, the original spectral data may contain noise and fluorescence background interference, making it more difficult to extract Raman spectral information from the original spectra. Especially the fluorescence background signal, which is typically several orders of magnitude stronger than the Raman signal, can even overwhelm or obscure the Raman signals, thereby impeding the qualitative or quantitative analysis of the Raman spectra. One effective method for removing the fluorescence background is shift excitation Raman differential spectroscopy (SERDS), which typically involves measuring two raw Raman spectra using slightly different excitation wavelengths, combined with reconstruction algorithms, to obtain Raman spectra free from fluorescence interference.

, a new leafhopper genus from northern North America, with a review of Oman (Hemiptera, Cicadellidae, Deltocephalinae).

The poorly known leafhopper species described as Deltocephalus (Laevicephalus) concinnus var. incisurus DeLong, 1926 previously had no accepted generic placement. It is here redescribed and placed in in the tribe Paralimnini, as (DeLong) Hamilton, 1987 is a junior of .

Phosphorylation of Zearalenone Retains Its Toxicity.

Microbial biotransformation of Zearalenone (ZEN) is a promising deactivation approach. The residual toxicity and stability of Zearalenone-14-phosphate (ZEN-14-P) and Zearalenone-16-phosphate (ZEN-16-P), two novel microbial phosphorylation products of ZEN, remain unknown. We investigated the cytotoxicity, oxidative stress, proinflammatory, and estrogenic activity of phosphorylated ZENs using porcine intestinal cells, uterine explants, and human endometrial cells and traced their metabolic fate by liquid chromatography-tandem mass spectrometry (LC-MS)/MS analysis.

The Path to Net-Zero in Dairy Production: Are Pronounced Decreases in Enteric Methane Achievable?

Achieving net-zero greenhouse gas (GHG) emissions in dairy production will require >50% reduction in enteric methane (CH) emissions together with elimination of emissions from feed production, additional carbon sequestration, reduction in manure emissions, anaerobic digestion of manure, and decreased reliance on fossil fuel energy. Over past decades, improved production efficiency has reduced GHG intensity of milk production (i.e.

Structural variation in the pangenome of wild and domesticated barley.

Pangenomes are collections of annotated genome sequences of multiple individuals of a species. The structural variants uncovered by these datasets are a major asset to genetic analysis in crop plants. Here we report a pangenome of barley comprising long-read sequence assemblies of 76 wild and domesticated genomes and short-read sequence data of 1,315 genotypes.

Turnip mosaic virus selectively subverts a PR-5 thaumatin-like, plasmodesmal protein to promote viral infection.

Pathogenesis-related (PR) proteins are induced by abiotic and biotic stresses and generally considered as part of the plant defense mechanism. However, it remains yet largely unclear if and how they are involved in virus infection. Our recent quantitative, comparative proteomic study identified three PR-5 family proteins that are significantly differentially accumulated in the plasmodesmata (PD)-enriched fraction isolated from Nicotiana benthamiana leaves infected by turnip mosaic virus (TuMV).

Global mRNA profiling reveals the effect of boron as a crop protection tool against .

, the causal agent of white mould, is a necrotrophic fungal pathogen responsible for extensive crop loss. Current control options rely heavily on the application of chemical fungicides that are becoming less effective and may lead to the development of fungal resistance. In the current study, we used a foliar application of boron to protect (canola) from infection using whole-plant infection assays.

Predictive accuracy of fecal calprotectin for histologic remission in ulcerative colitis.

Background/aims: Accurate assessment of disease activity is crucial for effective management and treatment of ulcerative colitis (UC). This study evaluated the correlation between clinical, endoscopic, and histologic measures of disease activity in UC.

Methods: Clinical, biochemical, endoscopic, and histologic disease activity was studied in 347 patients with UC.

Altered Metabolism in Knockdown Lines of Two HXXXD/BAHD Acyltransferases During Wound Healing in Potato Tubers.

Suberin biosynthesis involves the coordinated regulation of both phenolic and aliphatic metabolisms. HXXXD/BAHD acyltransferases occupy a unique place in suberization, as they function to crosslink phenolic and aliphatic monomers during suberin assembly. To date, only one suberin-associated HXXXD/BAHD acyltransferase, StFHT, has been described in potatoes, whereas, in , at least two are implicated in suberin biosynthesis.

Utilizing Short Interspersed Nuclear Element as a Genetic Marker for Pre-Harvest Sprouting in Wheat.

Pre-harvest sprouting (PHS) is a complex abiotic stress caused by multiple exogenous and endogenous variables that results in random but significant quality and yield loss at the terminal crop stage in more than half of the wheat-producing areas of the world. Systematic research over more than five decades suggests that addressing this challenge requires tools beyond the traditional genetic manipulation approach. Previous molecular studies indicate a possible role of epigenetics in the regulation of seed dormancy and PHS in crops, especially through RNA-directed DNA methylation (RdDM) pathways mediated by Argonaute (AGO) proteins.

Traditional Chinese medicine in treating upper digestive tract cancers.

Upper digestive tract cancers, such as oral cavity, laryngeal, esophageal, and gastric cancers, account for 10% of cancer cases and 14.5% of cancer-related deaths worldwide. Conventional treatments often provide limited survival benefits and are frequently associated with adverse effects and drug resistance.

Difference in Drinking Times as a Function of Liquid Consistency in Adults With Oculopharyngeal Muscular Dystrophy: A Comparative Study Using Bostwick Consistometer and IDDSI Flow Test Methods.

The main objective was to document the differences between drinking times and oral perception between liquids in individuals with dysphagia. A second objective was to assess variations in consistency categorization across instruments. A third objective was to explore the relationship between drinking time and dysphagia severity.

Quantification of cavitating flows with neutron imaging.

The current experimental investigation demonstrates the capability of neutron imaging to quantify cavitation, in terms of vapour content, within an orifice of an abruptly constricting geometry. The morphology of different cavitation regimes setting in was properly visualised owing to the high spatial resolution of 16 μm achieved, given the extensive field of view of 12.9 × 12.

Buffalo worm (Alphitobius diaperinus) proteins: Structural properties, proteomics and nutritional benefits.

Biophysical methods such as circular dichroism (CD) and differential scanning calorimetry (DSC) have been minimally used to characterize insect-derived proteins. This study examines the insect Alphitobius diaperinus as a potential protein source. Techniques such as alkaline solubilization coupled to isoelectric precipitation and Osborne fractionation were used to obtain protein concentrates and fractions (albumins, globulins, prolamins, glutelins).

From inflammation to immune regulation: The dual nature of dietary lectins in health and disease.

Beans, vegetables, fruits, and mushrooms offer a delightful array of fragrances and an abundance of nutrients, including essential vitamins, minerals, protein rich in vital amino acids, and omega-3 fatty acids. However, they may also contain lectins, carbohydrate-binding proteins with potential health risks. While some lectins exhibit stability and resistance to digestion, posing threats to gastrointestinal integrity and immune function, others, such as those from butterfly peas and pink bauhinia, show immunomodulatory properties that could bolster immune responses.

Limit of detection of ser. Enteritidis using culture-based versus culture-independent diagnostic approaches.

Unlabelled: To prevent the spread of foodborne illnesses, the presence of pathogens in the food chain is monitored by government agencies and food producers. The culture-based methods currently employed are sensitive but time- and labor-intensive, leading to increasing interest in exploring culture-independent diagnostic tests (CIDTs) for pathogen detection. However, few studies quantify the relative sensitivity and reliability of these CIDTs compared to current approaches.

Phosphor-in-Ceramic-Converted Laser-Driven Near-Infrared Light Sources for Multiple Intelligent Spectroscopy Applications.

Ultrabright broadband near-infrared (NIR) phosphor-converted laser diode (pc-LD) as a light source is increasingly essential for improving the sensitivity and spatial resolution of intelligent NIR spectroscopy technologies. However, the performance of NIR pc-LD is greatly hindered by the low external quantum efficiency (EQE) and poor thermal resistance of phosphor materials. Herein, a highly stable phosphor-in-ceramic (PiC) film deposited on high thermal conductivity substrate, in which the NIR-emitting CaMgHfGeO:Cr phosphor is incorporated into a glass-crystallized CaGaGeO ceramic matrix, along with the formation of a new type PiC composite material with high efficiency, absorbance, and thermal conductivity, is designed and prepared.

Multicellular tumor spheroids: A convenient in vitro model for translational cancer research.

In the attempts to mitigate uncertainties in the results of monolayer culture for the identification of cancer therapeutic targets and compounds, there has been a growing interest in using 3D cancer spheroid models, which include tumorospheres (TSs), tissue-derived tumor spheres (TDTSs), organotypic multicellular tumor spheroids (OMSs), and multicellular tumor spheroids (MCTSs). The MCTSs, either Mono-MCTSs or Hetero-MCTSs, with or without scaffold, in particular, offer numerous advantages over other spheroid models, including easy cultivation, high reproducibility, accessibility, high throughput, controllable size, well-rounded shape, simplicity of genetic manipulation, economical and availability of various biological methods for their development. In this review, we have attempted to discuss the role of MCTSs concerning various aspects of translational cancer research, such as drug response and penetration, cell-cell interaction, and invasion and metastasis.

Seeking balance between contradictory experiences - therapists treating survivors of sexual violence.

With the prevalence of sexual violence in most countries and its increase in Sweden, it is important to understand the development of secondary traumatic reactions among professionals who work with sexually violated clients. The aim of this study was to describe the meaning of therapists' personal experiences when treating survivors of sexual violence. We conducted a qualitative interview study of therapists in Sweden ( = 11) using thematic analysis and adding a phenomenological openess towards the phenomenon.

Dermacentor species (Acari: Ixodidae) in western Canada, with detection of Dermacentor similis.

Numerous tick species are undergoing significant range expansion in Canada, including several Dermacentor spp Koch (Acari: Ixodidae). With the recent description of Dermacentor similis Lado in the western United States, additional research is required to determine the current range of this species. Five hundred ninety-eight Dermacentor spp.

Enhancement of the formation and stability of low-fat Pickering emulsion gels stabilized with egg yolk granules-chitosan complex: Insights into the development of mayonnaise substitutes.

This study employed egg yolk granules (EYG) in combination with chitosan (CS) to evaluate the feasibility of constructing low-fat Pickering emulsion gels and investigated the properties of emulsion gels with different oil volume fractions (0-50 %) to address the stability issue. The results showed that the self-supporting structure of the emulsion gels gradually increased at 0-40 % oil phase, and the oil droplet sizes were all smaller than 10 μm. However, when the oil phase reached 50 %, the emulsion showed water-oil phase separation.