[Inguinal lymphadenitis due to invasion of Dirofilaria repens].

The paper presents the case of the right-sided inguinal inflammation of a lymph node as a result of invasion of Dirofilaria repens, the parasitic pathogen of subcutaneous dirofilariasis in animals of the canine family. The diagnosis was verified on the basis of the parallel application of morphological studies of cross sections of the nematode in histological samples and the molecular biological method polymerase chain reaction of scrapings of histological material. The localization of this helminth inside the cavities of the human body is extremely rare.

[Whooping cough in different periods of pregnancy].

Aim: To study the clinical course, diagnosis of pertussis in pregnant women.

Materials And Methods: Under observation were 25 pregnant women with whooping cough. The age structure was dominated by patients from 6 to 35 years old, amounting to 64%.

Description of the rare localization of Dirofilaria repens in human in the right inguinal lymph node.

D. repens is the causative agent of subcutaneous dirofilariasis in canine animals. In recent years, human invasion by this vector-borne helminthiasis has been recorded in many countries with temperate climate, but the localization of this helminth in the cavities of the human body is extremely rare.

Evaluating the frequency of carbapenem and aminoglycoside resistance genes among clinical isolates of from Ahvaz, south-west Iran.

is one of the most important opportunistic challenging pathogens as a result of its ability to acquire resistance to broad range of antibiotics and cause a variety of severe nosocomial infections. We investigated the frequency of the aminoglycoside-modifying enzymes (AMEs) and oxacillinase genes among clinical isolates of collected from hospitalized patients in Imam Khomeini Hospital, Ahvaz city, Iran. This prospective cross-sectional study was performed on 80 clinical isolates of collected from patients referred to Imam Khomeini Hospital in Ahvaz, Iran.

Aza-crown compounds synthesised by the self-condensation of 2-amino-benzyl alcohol over a pincer ruthenium catalyst and applied in the transfer hydrogenation of ketones.

A well-defined PNN-Ru catalyst was revisited to self-condense 2-aminobenzyl alcohol in forming a series of novel aza-crown compounds [aza-12-crown-3 (1), aza-16-crown-4 (2) and aza-20-crown-5 (3)]. All aza-crown compounds are separated and determined by NMR, IR, and ESI-MS spectroscopy as well as X-ray crystallography, indicating the saddle structure of 1 and the twisted 1,3-alternate conformation structure of 3. These aza-crown compounds have been explored to study ferric initiation of transfer hydrogenation (TH) of ketones into their corresponding secondary alcohols in the presence of 2-propanol with a basic t-BuOK solution, achieving a high conversion (up to 95%) by a ferric complex with 2 in a low loading (0.

Recent autochthonous cases of leishmaniasis in residents of the Republic of Dagestan, Russian Federation.

Eighty years after the last published record of human leishmaniasis from Dagestan, Russian Federation, we report two recent cases which were most probably acquired locally: one case of visceral leishmaniasis in a 2-year old child, and one cutaneous leishmaniasis case in a 39-year-old man co-infected with HIV, both resident in Dagestan.

Molecular Epidemiology of HIV-1 Subtype G in the Russian Federation.

Although HIV-1 subtype A has predominated in Russia since the end of the 20th century, other viral variants also circulate in this country. The dramatic outbreak of HIV-1 subtype G in 1988-1990 represents the origin of this variant spreading in Russia. However, full genome sequencing of the nosocomial viral variant and an analysis of the current circulating variants have not been conducted.

The application of proteomic methods (MALDI-toff MS) for studying protein profiles of some nematodes (dirofilaria and ascaris) for differentiating species.

Introduction: Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-toff MS) is a reliable method for diagnosing a number of bacterial and fungal infections. It is also effective as a method of rapid diagnosis of several parasitic agents. We used MALDI-toff MS to study the protein profiles of four nematodes: Dirofilaria repens, Dirofilaria.

Clinical and laboratory features of human dirofilariasis in Russia.

The article presents the results of the prospective study of 266 patients with dirofilariasis who received medical and diagnostic assistance in Rostov Scientific Research Institute of Microbiology and Parasitology in Rostov-on-Don, Russia from 2000 to 2016. We have assessed the features of the dynamics of epidemiology of this infection in several territories of the Russian Federation, depending on the social structure of patients. Immature females of dirofilaria were found most commonly in humans (82.

ADAPTATION OF HUMAN ROTAVIRUS STRAINS OF GROUP A TO THE REPRODUCTION IN PASSAGED CELL CULTURES.

The incidence of rotavirus gastroenteritis in the world still has no tendency to reduction. The development of an effective vaccine would reduce or, in the future, even defeat this highly contagious dangerous disease. However, both in Russia and abroad there is still no developed technique for adapting and cultivating strains of the human rotavirus A that would stably produce a high "yield" of virus progeny in transplanted culture cells.

[The prevalence and possibilities of serological diagnostic of reoviral infection.].

The information about manifestations and possible associations of reoviral infection is covered. The original results of studying prevalence, clinical significance and possibilities of serodiagnostic of reoviral infection under various pathological conditions. It is demonstrated that reoviral infection is widespread among adult population (60.

Periorbital Dirofilaria repens imported to Denmark: A human case report.

Dirofilaria repens, a filarial nematode of dogs and other carnivores, can accidentally infect humans. The infection occurs widely throughout Europe. We report a case of D.

Human subcutaneous/ocular dirofilariasis in the Russian Federation and Belarus, 1997-2013.

Objectives: This study was performed to determine the yearly frequency, trends in spread, and clinical-epidemiological characteristics of human subcutaneous/ocular dirofilariasis (HSOD) in the Russian Federation and Belarus.

Methods: The surveillance data of HSOD cases occurring between January 1997 and June 2013, obtained from the regional health administrations of the Russian Federation and Belarus, were analyzed. The spatial distribution of cases was assessed using a GIS map, and the clinical-epidemiological characteristics were defined using 719 cases for which information on anatomical location, age, sex, and area of residence was available.

Dirofilaria repens in the Russian Federation: current epidemiology, diagnosis, and treatment from a federal reference center perspective.

Background: Dirofilariasis is an emerging zoonotic infection in which the human serves as an accidental host. Russia has been one of the most important endemic regions worldwide for Dirofilaria repens, the most prevalent Dirofilaria species in Europe.

Methods: Based on a cohort of patients at the Rostov Medical University, we provide an epidemiological description of 236 human cases, reporting common clinical symptoms and treatment options for D.

[A novel laboratory method of isolation and purification of tobramycin].

A new laboratory method for isolation and purification of tobramycin by using extraction of a tobramycin derivative with benzaldehyde by methylene chloride, subsequent hydrolysis of azomethine and recrystallization of the formed tobramycin sulfate from solution of sulfuric acid in methanol was developed. The method allows to exclude the stage of chromatographic purification of tobramycin, to reduce the time of the process realization from 120-125 h to 15-20 h, to increase the yield of the target product from 37-40% to 60-65% without decreasing the product quality, to exclude a number of large-size and expensive equipment and to ensure high reproducibility of the technology.

[Listeria pathogenicity factors and their role in pathogenesis and laboratory diagnosis of listeriosis].

The mechanisms of relationships between Listeria and eukaryotic cells on the level of the main biomolecules are analyzed. These mechanisms determine the penetration and multiplication of Listeria and their importance for the pathogenesis of listeriosis. The theoretical and practical aspects of the regulation of the expression of pathogenicity factors in Listeria, connected with the activation of the regulatory protein PrfA and autoregulatory mechanisms, are considered.

[Persistence of pneumotrophic infective agents in acute bronchopulmonary diseases in children].

175 children with acute bronchopulmonary pathology were examined for the presence of the pneumotropic infective agents by serological and bacteriological methods. In most children microbial associations with the prevalence of mycoplasmas, pneumocycts and, to a lesser extent, chlamydiae were detected. The considerable activation of cytomegalovirus (CMV) infection in children with pneumonia and bronchitis was noted.

Quantitative assessment of the infection rate of the entomophthoraceous fungus, Zoophthora anhuiensis against the green peach aphid Myzus persicae.

A two-step method was developed to quantitatively assess the infection rate of the entomophthoraceous fungus, Zoophthora anhuiensis (Li) Humber, on the green peach aphid, Myzus persicae (Sulzer). Firstly, a standard time-dose-mortality relationship, established by modeling data from bioassay 1 at varying conidial dosages (0.4 - 10.

[Role of contagious infection of Pandora delphacis in suppression of Myzus persicae colonies].

The colonies of green peach aphid, Myzus persicae, on detached cabbage leaves (8 replicates) were initiated with each including 3 apterae exposed to 'spore shower' of the entomophthoraceous fungus, Pandora delphacis, to evaluate its potential for aphid control. The colonies were then allowed to freely propagate and infect from one to another for mycosis development at different regimes of temperature (10-30 degrees C) and relative humidity (74-100% RH). During a period of 30-day observation, aphid mycosis developed much more rapidly at the regimes of higher temperature (20-30 degrees C) and humidity (> or = 95% RH), at which, nymphs were effectively infected by contacting the conidia discharged from apterous cadavers.

[Effect of drying and temperature on the sporulation of Pandora delphacis mycelia gelatinized].

The entomophthoraceous fungus, Pandora delphacis, is a microbial agent highly potential for control of sucking-type insects. In this study, effort was made of gelatinizing the mycelia of the isolate F95129 from submerged culture using polyacrylamide-starch gel pwder and sodium alginate. The resulting film-like gel of the mycelia sporulated very well, indicating that the materials used for gelatinization of the mycelia was biologically compatible with P.

[Biomass, sporulation and aphid-infecting virulence of Pandora neoaphidis mycelia produced in repeated liquid culture].

Repeated culture of entomophthoraceous isolates is a suspectable factor leading to their virulence decline of biological variation. In the present study, an isolate of Pandora neoaphidis, F98028, was repeatedly cultured six times at the regime of 20 degrees C and 80 r/min in Sabouraud dextrose broth(SDB) at three inoculation ratio of 1/20, 2/20, and 4/20 (seed culture over fresh SDB). Mycelial biomass(MB), sporulation capacity(SC), and virulence to the green peach aphid, Myzus persicae, were assessed for each of the subcultures.

[Variation in the viability and the metabolism of internally reserved nutrients of Beauveria bassiana conidia during storage at different regimes of water content and temperature].

While the fresh powder (with water content of 58.9 +/- 1.6%) and vacuum-freeze dried (VFD) one (with water content of 7.