Comparison of the nucleotide sequence and secondary structure of the 5.8S ribosomal RNA gene of Chlamydomonas tetragama with those of green algae.

We have determined the nucleotide sequence of a PCR product corresponding to the 5.8S ribosomal RNA gene in Chlamydomonas tetragama and compared the obtained sequence with those of one red and eleven green algae. A phylogenetic tree based on the 5.

Detection, localization, and sequence analyses of mitochondrial regulatory region RNAs in several mammalian species.

The mitochondrial regulatory region (mrr) located between the tRNAPhe and tRNAPro genes of mitochondrial DNA (mtDNA) is essential for regulation of replication and transcription of the mitochondrial genome. Polyadenylated short RNAs complementary to the L-strand of the mrr in human cells and similar RNAs (polyadenylation status unknown) in rat and mouse cells have been reported. We now report detection of ca.

A Non-radioassay of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase by Anion-Exchange Chromatography.

A non-radioisotopic assay method for ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is described. 3-Phosphoglycerate produced by the RuBisCO reaction was measured using an anion-exchange chromatography. The reaction course of spinach RuBisCO measured by this method was completely the same as that measured with (14)CO2.

Ribulose-1,5-bisphosphate carboxylase/oxygenase from thermophilic red algae with a strong specificity for CO2 fixation.

Strongly carboxylase-specific ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) was found in Galdieria partita and Cyanidium caldarium (Cyanidiophyceae). The relative specificity, VcKo/VoKc, of Galdieria and Cyanidium RuBisCO was 238 and 222, respectively; 2.4 to 2.

Modeling of continuously and directly analyzed biphasic reaction courses of ribulose 1,5-bisphosphate carboxylase/oxygenase.

This paper aims at clarifying the cause of the time-dependent, partial loss of the activity during reaction (so-called fallover) of ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from plant sources. This was done by comparing the reaction courses calculated using the reaction models constructed here based on the present conflicting two ideas on fallover with directly measured courses obtained with RuBisCO purified from spinach leaves. Since the ordinary methods with 14CO2 and indicator enzymes were not adequate for analyzing the progress of fallover, we followed the reaction by measuring the change of the light absorbance of ribulose 1,5-bisphosphate (RuBP) at 280 nm.

Microbial synthesis and properties of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in Comamonas acidovorans.

Comamonas acidovorans DS-17 was isolated from activated sludge and found to produce copolymers of 3-hydroxybutyrate (3HB) and 4-hydroxybutyrate (4HB) at 30 degrees C under growth-limited conditions. When 1,4-butanediol or 4-hydroxybutyric acid was used as the sole carbon source, a P(4HB) homopolymer was produced. Random copolymers of 3HB and 4HB units were produced on the addition of glucose or 3-hydroxybutyric acid to the culture solution of 4-hydroxybutyric acid.

Biosynthesis of poly(3-hydroxy-alkanoates) in Pseudomonas aeruginosa AO-232 from 13C-labelled acetate and propionate.

Pseudomonas aeruginosa AO-232 produced poly(3-hydroxyalkanoates) (P(3HA)) containing monomer units of even carbon numbers C6, C8, C10 and C12, when sodium acetate was fed as the sole carbon source. In contrast, the P(3HA) produced from sodium propionate was composed of seven different 3HA units ranging from C6 to C12. The pathways of P(3HA) synthesis were investigated by using 13C-labelled acetate and propionate as the carbon sources.