104 results match your criteria: "Research Institute of General Reanimatology[Affiliation]"
Mutat Res
May 2017
Research Centre for Medical Genetics, 115478 Moscow, 1 Moskvorechye str., Russia; V. A. Negovsky Research Institute of General Reanimatology, Moscow, 107031, Russia.
Oxid Med Cell Longev
March 2017
Research Centre for Medical Genetics (RCMG), Moscow 115478, Russia.
The influence of a water-soluble [60] fullerene derivative containing five residues of 3-phenylpropionic acid and a chlorine addend appended to the carbon cage (F-828) on serum-starving human embryo lung diploid fibroblasts (HELFs) was studied. Serum deprivation evokes oxidative stress in HELFs. Cultivation of serum-starving HELFs in the presence of 0.
View Article and Find Full Text PDFMutat Res Genet Toxicol Environ Mutagen
July 2016
Research Centre for Medical Genetics (RCMG), Moscow, 115478, Russia.
Water-soluble fullerenes have been studied as potential nanovectors and therapeutic agents, but their possible toxicity is of concern. We have studied the effects of F-828, a soluble fullerene [C60] derivative, on diploid human embryonic lung fibroblasts (HELFs) in vitro. F-828 causes complex time-dependent changes in ROS levels.
View Article and Find Full Text PDFCrit Care
March 2016
Centre for Heart Lung Innovation, St. Paul’s Hospital, University of British Columbia, 1081 Burrard Street, Vancouver, BC V6Z 1Y6 Canada
Ter Arkh
March 2016
S.N. Vinogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia.
Healthcare-associated infections (HCAI) remain one of the most challenges of modern health care and assume increasing social and medical significance. The specific features of HCAI are frequent recurrences and inefficiency of antibiotic therapy, a reason for which is antibiotic resistance in microorganisms. The review discusses antibiotic resistance, a form of antibiotic tolerance (AT), and its role in the development of HCAI.
View Article and Find Full Text PDFInt J Pharm
January 2016
V.A. Negovsky Scientific Research Institute of General Reanimatology, Moscow, Russian Federation.
Here we show that methemoglobin is converted to oxyhemoglobin in the presence of perfluorocarbon (PFС) emulsion. Methemoglobin in blood at the level of above 30% can cause severe complications and lethal outcome. Some pharm chemicals in blood in vivo and in vitro can lead to oxidation of iron, Fe(2+)→Fe(3+), and to increased level of methemoglobin.
View Article and Find Full Text PDFAm J Emerg Med
December 2015
Hellenic Society of Cardiopulmonary Resuscitation, Athens, Greece; European University of Cyprus, School of Medicine, Nicosia, Cyprus.
Cytochrome c, an electron carrier that normally resides in the mitochondrial intermembrane space, may translocate to the cytosol under ischemic and hypoxic conditions and contribute to mitochondrial permeability transition pore opening. In addition, reperfusion of brain tissue following ischemia initiates a cell death cascade that includes cytochrome c-mediated induction of apoptosis. Further studies are needed to determine the contribution of cytochrome c in the regulation of cell death, as well as its value as an in vivo prognostic marker after cardiac arrest and resuscitation.
View Article and Find Full Text PDFDisorders in the erythrocyte morphology and structure of their membranes during long-term storage of erythrocyte suspension (30 days at 4°C) were studied by atomic force microscopy. The morphology and nanostructure of erythrocyte membranes, biochemical parameters, ion exchange parameters, and hemoglobin spectra were recorded. The transformation of erythrocyte morphology and destruction of their membranes were observed throughout the storage period.
View Article and Find Full Text PDFExp Cell Res
October 2015
V.A. Negovsky Scientific Research Institute of General Reanimatology, Moscow, Russian Federation.
Packed red blood cells (PRBC) are used for blood transfusion. PRBC were stored for 30 days under 4 °С in hermetic blood bags with CPD anticoagulant-preservative solution. Hematocrit was 50-55%.
View Article and Find Full Text PDFWorld J Gastroenterol
July 2015
Vasiliy Ivanovich Reshetnyak, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia.
Primary biliary cirrhosis (PBC) is a chronic progressive cholestatic granulomatous, and destructive inflammatory lesion of small intralobular and septal bile ducts, which is likely to be caused by an autoimmune mechanism with a the presence of serum antimitochondrial antibodies and a potential tendency to progress to cirrhosis. Despite the fact that the etiology of this disease has been unknown so far, there has been a considerable body of scientific evidence that can reveal the clinical and laboratory signs of PBC and the individual components of its pathogenesis and elaborate diagnostic criteria for the disease and its symptomatic therapy. Deficiencies in autoimmune tolerance are critical factors for the initiation and perpetuation of the disease.
View Article and Find Full Text PDFBiochemistry (Mosc)
March 2015
Negovsky Research Institute of General Reanimatology, Moscow, 107031, Russia.
Previous studies showed that large amounts of phenylcarboxylic acids (PhCAs) are accumulated in a septic patient's blood due to increased endogenous and microbial phenylalanine and tyrosine biotransformation. Frequently, biochemical aromatic amino acid transformation into PhCAs is considered functionally insignificant for people without monogenetic hereditary diseases. The blood of healthy people contains the same PhCAs that are typical for septic patients as shown in this paper.
View Article and Find Full Text PDFClin Exp Immunol
June 2015
N. I. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.
Host genetic variations may influence a changing profile of biochemical markers and outcome in patients with trauma/injury. The objective of this study was to assess clinical associations of single nucleotide polymorphisms (SNPs) in the genes of cytokines in critically ill patients. A total of 430 patients were genotyped for SNPs in the genes of pro- (IL1B, IL6, IL8) and anti-inflammatory (IL4, IL10, IL13) cytokines.
View Article and Find Full Text PDFHemin is the product of hemoglobin oxidation. Some diseases may lead to a formation of hemin. The accumulation of hemin causes destruction of red blood cells (RBC) membranes.
View Article and Find Full Text PDFWorld J Gastroenterol
November 2013
Vasiliy Ivanovich Reshetnyak, V.A. Negovsky Scientific Research Institute of General Reanimatology, Russian Academy of Medical Sciences, 107031 Moscow, Russia.
This review considers the physiological and molecular biochemical mechanisms of bile formation. The composition of bile and structure of a bile canaliculus, biosynthesis and conjugation of bile acids, bile phospholipids, formation of bile micellar structures, and enterohepatic circulation of bile acids are described. In general, the review focuses on the molecular physiology of the transporting systems of the hepatocyte sinusoidal and apical membranes.
View Article and Find Full Text PDFJ Biomed Sci
October 2012
Negovsky Research Institute of General Reanimatology Russian Academy of Medical Sciences, Laboratory of Metabolism of Critical State, Moscow, Russia.
Background: Several low-molecular-weight phenolic acids are present in the blood of septic patients at high levels. The microbial origin of the most of phenolic acids in the human body was shown previously, but pathophysiological role of the phenolic acids is not clear. Sepsis is associated with the excessive production of reactive oxygen species (ROS) in both the circulation and the affected organs.
View Article and Find Full Text PDFAtomic force microscopy (AFM) allows a researcher to obtain images of red blood cells (RBC) and their membranes. Various effects on blood lead to surface alterations of cell membranes. Such alterations are estimated by a corrugation of membrane surface.
View Article and Find Full Text PDFActa Bioeng Biomech
November 2012
V. A. Negovsky Scientific Research Institute of General Reanimatology, Russian Academy of Medical Sciences, Moscow, Russia.
The goal of the study was to investigate the effects of zinc ions of various concentrations on the nanostructure of membrane of red blood cells in in vitro experiment. The suspension of red blood cells extracted from whole human blood was used. The calibrated electroporation and the atomic force microscopy (AFM) were used to analyse damage to membrane nanostructure.
View Article and Find Full Text PDFWorld J Hepatol
February 2012
Vasiliy Ivanovich Reshetnyak, VA Negovsky Scientific Research Institute of General Reanimatology, Russia Academy of Medical Sciences, Moscow 107031, Russia.
Gallstone disease (GD) is a chronic recurrent hepatobiliary disease, the basis for which is the impaired metabolism of cholesterol, bilirubin and bile acids, which is characterized by the formation of gallstones in the hepatic bile duct, common bile duct, or gallbladder. GD is one of the most prevalent gastrointestinal diseases with a substantial burden to health care systems. GD can result in serious outcomes, such as acute gallstone pancreatitis and gallbladder cancer.
View Article and Find Full Text PDFSemin Cardiothorac Vasc Anesth
December 2010
V. A. Negovsky Research Institute of General Reanimatology of the Russian Academy of Medical Sciences, Moscow, Russian Federation.
The authors studied changes in erythrocyte membrane nanostructure using a rodent model of hemorrhagic hypotension and resuscitation. Both macro- and microstructural elements were examined using atomic force microscopy. Membrane "roughness" was characterized using spatial Fourier transformation and was stratified according to the periodicity of the membrane.
View Article and Find Full Text PDFSemin Cardiothorac Vasc Anesth
December 2010
V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russian Federation.
The objective of this study was to measure the efficacy of biphasic positive airway pressure (BIPAP) and synchronized intermittent mandatory ventilation (SIMV) with alveolar recruitment maneuvers (ARMs) in patients with acute lung injury (ALI) and concomitant pneumothorax. Seventy-four patients with ALI and concomitant pneumothorax secondary to blunt thoracic injury were studied. All patients fulfilled criteria for the first stage of acute respiratory distress syndrome, which consisted of acute onset dyspnea, isolated rales, an extravascular lung water index >7 mL/kg, and an oxygenation index <300 mm Hg in the absence of left-ventricular dysfunction.
View Article and Find Full Text PDFSemin Cardiothorac Vasc Anesth
December 2010
V.A. Negovsky Research Institute of General Reanimatology, Moscow, Russian Federation.
Acute respiratory distress syndrome (ARDS) complicates nosocomial pneumonias (NPn) in 12% to 33% of patients with associated increases in mortality of up to 80%. A timely diagnosis of ARDS with NPn is, however, problematic. The aim of this investigation was to improve the diagnosis and treatment of the early stages of ARDS with NPn.
View Article and Find Full Text PDFSemin Cardiothorac Vasc Anesth
December 2010
V. A. Negovsky Research Institute of General Reanimatology, Novokuznetsk, Russian Federation.
The objective of the present study was to examine the relationship among extravascular pulmonary water, intracranial and cerebral perfusion pressure, hemodynamic parameters (eg, cardiac index, system vascular resistance index), and brain stem function during acute ischemic stroke. The subjects were 17 comatose patients with ischemic stroke who were admitted to an intensive care unit. The results revealed an elevation in extravascular lung water in the absence of cardiac dysfunction.
View Article and Find Full Text PDFJ Crit Care
September 2010
V.A. Negovsky Research Institute of General Reanimatology, Russian Academy of Medical Sciences, Moscow, Russia.
Purpose: After the influence of different actions on the blood, the erythrocytes may change their macrostructure. At the same time, the microstructure of cell membrane will be changed as well. This study provides the results of comparison of red blood cell membrane microstructure after they have been affected by different factors.
View Article and Find Full Text PDFWorld J Gastroenterol
August 2008
Scientific Research Institute of General Reanimatology, Russia Academy of Medical Sciences, Moscow 107031, Russia.
A number of new hepatitis viruses (G, TT, SEN) were discovered late in the past century. We review the data available in the literature and our own findings suggesting that the new hepatitis G virus (HGV), disclosed in the late 1990s, has been rather well studied. Analysis of many studies dealing with HGV mainly suggests the lymphotropicity of this virus.
View Article and Find Full Text PDFResuscitation
October 2007
Research Institute of General Reanimatology, Russian Academy of Medical Sciences, Moscow, Russia.