Enhancing of Rabbit Sperm Cryopreservation with Antioxidants Mito-Tempo and Berberine.

Cryopreservation plays a critical role in animal breeding and the conservation of endangered species, but it often compromises sperm characteristics such as morphology, motility, and viability due to oxidative stress. This study explores the antioxidative effect of Mito-Tempo (MT) and Berberine (BER) to enhance post-thaw sperm quality in rabbits. Pooled rabbit sperm samples were supplemented with different concentrations (0.

Toxic and essential elements in honeybee venom from Slovakia: Potential health risk to humans.

Honeybee venom is one of the natural substances produced by bees (). Their venom gland produces venom which plays a defensive role. In this study a concentration of macro and trace elements (Ag, Al, As, Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, Sb, Se, Sr, Pb and Zn) in foragers' and honeybees' venom was analysed by axial inductively coupled plasma optical emission spectrometry (ICP OES) with good validation parameters to differentiate the element accumulation ability in honeybee venom.

Interplay Between the MicroRNA miR-152 and Quercetin in the Control of Ovarian Granulosa Cell Functions.

In the present study, we examined the functional interrelationships between microRNAs and plant polyphenols in the regulation of ovarian cell functions. For this purpose, we compared the basic functions of porcine ovarian granulosa cells with or without transfection with miR-152 mimics that were cultured with or without quercetin. The expression levels of miR-152, cell viability, cell proliferation (accumulation of proliferating cell nuclear antigen, PCNA), apoptosis (accumulation of Bax) and the release of progesterone, estradiol, and insulin-like growth factor I (IGF-I) were analyzed by real-time quantitative polymerase chain reaction (RT‒qPCR), the Trypan blue exclusion test, quantitative immunocytochemistry, and enzyme-linked immunosorbent assays (ELISAs).

The Effects of Adding Hempseed Cake on Sperm Traits, Body Weight, Haematological and Biochemical Parameters in Rabbit Males.

Incorporating of agro-industrial co-products into animal nutrition could represent an opportunity to lessen the environmental impact of the food production chain. One such co-product is a hempseed cake originating from cold pressing hemp seeds to extract oil for human consumption. The aim of the present study was to evaluate the action of hempseed cake in the diet on male rabbit reproductive and some non-reproductive indexes.

Honey bees for pesticide monitoring in the landscape: Which bee matrices should be used?

Among bee species, the western honey bee (Apis mellifera) is preferred in monitoring studies performed in the agricultural landscape, while bee matrices, pollen, and honey are mostly a subject of these studies due to their unique composition. A justified question about the relevance of other bee matrices, like larvae, foragers, beebread, and/or wax, has been raised. The ability of different bee matrices (wax, pollen grains, bee bread, foragers, larvae, nectar, and honey) to absorb pesticide residues is subjected in this study.

Factors affecting cryotolerance of mammalian oocytes.

Cryopreservation of oocytes is an important tool for preserving genetic resources and for farm animals breeding. Processes taking place during vitrification affect oocytes and result in their reduced developmental capacity and lower fertilisation rates of cryopreserved oocytes. Further improvement in cryopreservation techniques is still required.

Nanoparticle-plant interactions: Physico-chemical characteristics, application strategies, and transmission electron microscopy-based ultrastructural insights, with a focus on stereological research.

Ensuring global food security is pressing among challenges like population growth, climate change, soil degradation, and diminishing resources. Meeting the rising food demand while reducing agriculture's environmental impact requires innovative solutions. Nanotechnology, with its potential to revolutionize agriculture, offers novel approaches to these challenges.

The microRNA miR-152 can mitigate and prevent the toxic effect of benzene on porcine ovarian cells.

Epigenetic methods to prevent the reproductive toxicity of oil-related environmental contaminants are currently unavailable. The present study aimed to examine the ability of the microRNA miR-152 to mitigate the effects of benzene on ovarian cells. Porcine ovarian granulosa cells transfected or not transfected with miR-152 mimics were cultured with or without benzene (0, 10 and 100 ng/ml).

Body weight prediction of Belgian Blue crossbred using random forest.

Objective: The aim of this study was to predict the body weight (BW) of a Belgian Blue X Friesian Holstein (BB X FH) crossbred in Indonesia based on morphometrics using random forest.

Materials And Methods: A total of 26 BB X FH crossbreds were observed for BW, chest weight (CW), body length (BL), hip height (HH), wither height (WH), and chest girth (CG) from 0, 30, 60, 90, 120, 150, 180, 210, 240, 270, and 300 days of age. Stepwise regression and random forest were performed using R 3.

Genetic diversity of Cryptosporidium spp., Encephalitozoon spp. and Enterocytozoon bieneusi in feral and captive pigeons in Central Europe.

Cryptosporidium spp., Enterocytozoon bieneusi and Encephalitozoon spp. are the most common protistan parasites of vertebrates.

Association between blood hormones and fecundity in rabbit does.

The aim of the present study was to examine the association between blood hormone level and fecundity in rabbit does. The ovulation of nulliparous New Zealand White rabbits was induced by administration of PMSG and LH-RH analogue. Next day, the does were inseminated, blood was collected and the plasma levels of IGFI, leptin, progesterone and estradiol were measured by radioimmunoassay.

Modulatory effect of pomegranate peel extract on key regulators of ovarian cellular processes .

In this study, response of ovarian cells (human granulosa cell line HGL5, and human adenocarcinoma cell line OVCAR-3) to short-term pomegranate peel extract (PPE) treatment (for 24 hours in cell culture) was evaluated . Quantitative and qualitative screening of polyphenols revealed punicalagins α and β as major polyphenolic components. Total phenolic content (TPC) was 93.

Population diversity, admixture, and demographic trend of the Sumba Ongole cattle based on genomic data.

Objective: Sumba Ongole (SO) cattle are valuable breed due to their important role in the development of Indonesian cattle. Despite rapid advances in molecular technology, no genomic studies on SO cattle have been conducted to date. The aim of this study is to provide genomic profile related to the population diversity, admixture, and demographic trends of SO cattle.

Hormonal indexes as predictors of porcine reproductive traits.

The aim of the present study was to examine the applicability of several hormonal indexes for early prediction of puberty and reproductive state in pigs. For this purpose, we have compared the level of hormones leptin, estradiol, progesterone, and IGF-I in the blood of gilts at 150 days of age and their indexes of puberty and ovarian state at the age of 200 days. The association between blood leptin, estradiol, progesterone, and IGF-I and indexes of future reproductive state has been demonstrated.

The Influence of Heat Stress on Chicken Immune System and Mitigation of Negative Impacts by Baicalin and Baicalein.

Heat stress (HS) in poultry husbandry is an important stressor and with increasing global temperatures its importance will increase. The negative effects of stress on the quality and quantity of poultry production are described in a range of research studies. However, a lack of attention is devoted to the impacts of HS on individual chicken immune cells and whole lymphoid tissue in birds.

The morphological and functional ultrastructure of cells in pre-implantation embryos.

Cells of pre-implantation embryos are equipped with many morphological and functional systems through which they can synthesize specific proteins and effectively ensure the protection of early embryonic development. Here we present evidence for the existence of these systems in morphologically normal and abnormal bovine blastocyst stage embryos in vivo at the ultrastructural and actin cytoskeleton levels. The appearance of organelles in the trophectoderm (TE) and inner cell mass (ICM) cells, responsible for their synthetic activities and their role in the development of early bovine embryos are described.

Development and evaluation of a fish feed mixture containing the probiotic prepared using an innovative pellet coating method.

Introduction: Due to the intensification of fish farming and the associated spread of antimicrobial resistance among animals and humans, it is necessary to discover new alternatives in the therapy and prophylaxis of diseases. Probiotics appear to be promising candidates because of their ability to stimulate immune responses and suppress the growth of pathogens.

Methods: The aim of this study was to prepare fish feed mixtures with various compositions and, based on their physical characteristics (sphericity, flow rate, density, hardness, friability, and loss on drying), choose the most suitable one for coating with the selected probiotic strain R2 Biocenol™ CCM 8674 (new nom.

Feeding-Regime-Dependent Intestinal Response of Rainbow Trout after Administration of a Novel Probiotic Feed.

Intensive fish farming is associated with a high level of stress, causing immunosuppression. Immunomodulators of natural origin, such as probiotics or phytoadditives, represent a promising alternative for increasing the immune function of fish. In this study, we tested the autochthonous trout probiotic strain R2 in a newly developed, low-cost application form ensuring the rapid revitalization of bacteria.

MicroRNA miR-152 can support ovarian granulosa cell functions and modify apigenin actions.

The study aimed to evaluate the involvement of apigenin, microRNA (miR)-152, and their interrelationships in the control of basic ovarian granulosa cell functions. The effects of apigenin (0, 10, and 100 µg/mL), miR-152 analogues or miR-152 inhibitor, and their combinations with apigenin on porcine granulosa cells were examined. Expression levels of miR-152, viability, proliferation, apoptosis, steroid hormones, IGF-I, oxytocin, and prostaglandin E2 release were analyzed.

Mesenchymal stem cells of Oravka chicken breed: promising path to biodiversity conservation.

Mesenchymal stem cells (MSCs) are multilineage cells able to differentiate into other cell types. MSCs derived from bone marrow or compact bones are the most accessible stem cells used in tissue engineering. Therefore, the aim of this study was to isolate, characterize and cryopreserve MSCs of endangered Oravka chicken breed.

Pathogens in milk of goats and their relationship with somatic cell count.

We evaluated the presence of bacterial pathogens in the milk of goats and their relationship with somatic cell count (SCC) and milk composition. The study was performed on a dairy farm in northern Slovakia. Half udder milk samples were collected from goats in June and July.

Modifications in Ultrastructural Characteristics and Redox Status of Plants under Environmental Stress: A Review.

The rate of global environmental change is unprecedented, with climate change causing an increase in the oscillation and intensification of various abiotic stress factors that have negative impacts on crop production. This issue has become an alarming global concern, especially for countries already facing the threat of food insecurity. Abiotic stressors, such as drought, salinity, extreme temperatures, and metal (nanoparticle) toxicities, are recognized as major constraints in agriculture, and are closely associated with the crop yield penalty and losses in food supply.

Colored stains: Microbial survey of cellulose-based and lignin rich papers.

Over the centuries, various types of paper have been produced, each characterized by a different ratio of natural macromolecules, mainly lignin and cellulose. Handmade paper has a higher content of cellulose with respect to the early machine-made paper, where lignin is the other important component. Microorganisms are able to colonize and deteriorate both types.