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To evaluate the contribution of local pulsed heating of light-absorbing microregions to biochemical activity, irradiation of Escherichia coli was carried out using femtosecond laser pulses (lambda = 620 nm, tau p = 3 x 10(-13) S, fp = 0.5 Hz, Ep = 1.1 x 10(-3) J cm-2, Iav = 5.

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