[Interleukin-5-induced generation of superoxide by eosinophils in asthma].

Superoxide generation was assessed in highly purified eosinophils (> 99.0% pure) with a flow cytometer. Eosinophils from patients with asthma were collected by centrifugation of leukocytes on Percoll density gradients, followed by negative immunoselection with anti-CD16 monoclonal antibodies.

Enzymatic dispersion of mast cells from human sinus mucosa: characterization of histamine release and its comparison with chopped fragments of the tissue.

Functional characteristics of mast cells in chopped fragments from sinus mucosa, which was dissected from patients with chronic sinusitis, were compared with those from dispersed cells prepared by enzymatic treatment. The results obtained in this study were the following. (1) Both chopped fragments and dispersed cells released histamine in a dose-dependent manner when incubated with anti-IgE.