Identification of Anti-Tuberculosis Drugs Targeting DNA Gyrase A and Serine/Threonine Protein Kinase PknB: A Machine Learning-Assisted Drug-Repurposing Approach.

Tuberculosis (TB) is a global health challenge associated with considerable levels of illness and mortality worldwide. The development of innovative therapeutic strategies is crucial to combat the rise of drug-resistant TB strains. DNA Gyrase A (GyrA) and serine/threonine protein kinase (PknB) are promising targets for new TB medications.

Detecting Tumor-Associated Intracranial Hemorrhage Using Proton Magnetic Resonance Spectroscopy.

Intracranial hemorrhage associated with primary or metastatic brain tumors is a critical condition that requires urgent intervention, often through open surgery. Nevertheless, surgical interventions may not always be feasible due to two main reasons: (1) extensive hemorrhage can obscure the underlying tumor mass, limiting radiological assessment; and (2) intracranial hemorrhage may occasionally present as the first symptom of a brain tumor without prior knowledge of its existence. The current review of case studies suggests that advanced radiological imaging techniques can improve diagnostic power for tumoral hemorrhage.

Animal Models of Intervertebral Disc Diseases: Advantages, Limitations, and Future Directions.

Animal models are valuable tools for studying the underlying mechanisms of and potential treatments for intervertebral disc diseases. In this review, we discuss the advantages and limitations of animal models of disc diseases, focusing on lumbar spinal stenosis, disc herniation, and degeneration, as well as future research directions. The advantages of animal models are that they enable controlled experiments, long-term monitoring to study the natural history of the disease, and the testing of potential treatments.

Enhanced Performance and Durability of Pore-Filling Membranes for Anion Exchange Membrane Water Electrolysis.

Four distinct pore-filling anion exchange membranes (PFAEMs) were prepared, and their mechanical properties, ion conductivity, and performance in anion exchange membrane water electrolysis (AEMWE) were evaluated. The fabricated PFAEMs demonstrated exceptional tensile strength, which was approximately 14 times higher than that of the commercial membrane, despite being nearly half as thin. Ion conductivity measurements revealed that acrylamide-based membranes outperformed benzyl-based ones, exhibiting 25% and 41% higher conductivity when using crosslinkers with two and three crosslinking sites, respectively.

Effect of Anion-Conducting Electrolytes in Pore-Filling Membranes on Performance and Durability in Water Electrolysis.

This study examines the effect of the structural characteristics of anion-conducting monomers within pore-filling anion exchange membranes on the performance and durability of anion exchange membrane water electrolysis. Analysis reveals that acrylamide- and acrylate-based membranes show optimal performance without methyl groups, with acrylamide-based membranes outperforming their acrylate counterparts in current density, particularly at 1.8 V.

System Dynamics Modeling of Scale Formation in Membrane Distillation Systems for Seawater and RO Brine Treatment.

To overcome the limitations of traditional Reverse Osmosis (RO) desalination, Membrane Distillation (MD) has gained attention as an effective solution for improving the treatment of seawater and RO brine. Despite its potential, the formation of inorganic scales, particularly calcium sulfate (CaSO), continues to pose a major challenge. This research aims to explore the scaling mechanisms in MD systems through a combination of experimental analysis and dynamic modeling.

Evaluating Nanofiltration and Reverse Osmosis Membranes for Pharmaceutically Active Compounds Removal: A Solution Diffusion Model Approach.

Trace organic contaminants (TrOCs), including pharmaceutically active compounds (PhACs), present significant challenges for conventional water treatment processes and pose potential risks to environmental and human health. To address these issues, nanofiltration (NF) and reverse osmosis (RO) membrane technologies have gained attention. This study aims to evaluate the performance of NF and RO membranes in removing TrOCs from wastewater and develop a predictive model using the Solution Diffusion Model.

Detection of miR-133a-5p Using a Molecular Beacon Probe for Investigating Postmortem Intervals.

When a body is discovered at a crime or murder scene, it is crucial to examine the body and estimate its postmortem interval (PMI). Accurate estimation of PMI is vital for identifying suspects and providing clues to resolve the case. MicroRNAs (miRNAs or miRs) are small non-coding RNAs that remain relatively stable in the cell nucleus even after death-related changes occur.

Harnessing Radiation for Nanotechnology: A Comprehensive Review of Techniques, Innovations, and Application.

Nanomaterial properties such as size, structure, and composition can be controlled by manipulating radiation, such as gamma rays, X-rays, and electron beams. This control allows scientists to create materials with desired properties that can be used in a wide range of applications, from electronics to medicine. This use of radiation for nanotechnology is revolutionizing the way we design and manufacture materials.

Electron Holography for Advanced Characterization of Permanent Magnets: Demagnetization Field Mapping and Enhanced Precision in Phase Analysis.

This review explores a method of visualizing a demagnetization field () within a thin-foiled NdFeB specimen using electron holography observation. Mapping the is critical in electron holography as it provides the only information on magnetic flux density. The map within a NdFeB thin foil, derived from this method, showed good agreement with the micromagnetic simulation result, providing valuable insights related to coercivity.

Numerical Study on the Static Bending Response of Cracked Wind Turbine Blades Reinforced with Graphene Platelets.

With the growing demand for wind energy, the development of advanced materials for wind turbine support structures and blades has garnered significant attention in both industry and academia. In previous research, the authors investigated the incorporation of graphene platelets (GPLs) into wind turbine blades, focusing on the structural performance and cost-effectiveness relative to conventional fiberglass composites. These studies successfully demonstrated the potential advantages of GPL reinforcement in improving blade performance and reducing the blade's weight and costs.

Valence State and Catalytic Activity of Ni-Fe Oxide Embedded in Carbon Nanotube Catalysts.

The catalytic activity of Ni-Fe oxide embedded in CNTs was investigated in terms of valence states and active oxygen species. Ni-Fe oxides were prepared by the sol-gel combustion process, and Ni-Fe oxides embedded in CNT catalysts were synthesized by the catalytic chemical vapor deposition (CCVD) method. The lattice structure of the Ni-Fe oxide catalysts was analyzed, and the lattice distortion was increased with the addition of Fe.

Extract and Its Active Metabolite α-Cyperone Alleviates Paclitaxel-Induced Neuropathic Pain via the Modulation of the Norepinephrine Pathway.

Background: Paclitaxel is a widely used anticancer drug for ovarian, lung, breast, and stomach cancers; however, its clinical use is often limited by the side effects of peripheral neuropathy. This study evaluated the effects of () extract and its active metabolite, α-cyperone, on paclitaxel-induced neuropathic pain.

Methods: The oral administration of extract at doses of 500 mg/kg and intraperitoneal administration of α-cyperone at doses of 480 and 800 μg/kg prevented both the development of cold and mechanical pain.

The Role of Diet, Additives, and Antibiotics in Metabolic Endotoxemia and Chronic Diseases.

: Dietary patterns, including high-fat and high-carbohydrate diets (HFDs and HCDs), as well as non-dietary factors such as food additives and antibiotics, are strongly linked to metabolic endotoxemia, a critical driver of low-grade chronic inflammation. This review explores the mechanisms through which these factors impair intestinal permeability, disrupt gut microbial balance, and facilitate lipopolysaccharide (LPS) translocation into the bloodstream, contributing to metabolic disorders such as obesity, type 2 diabetes mellitus, and inflammatory bowel disease. : The analysis integrates findings from recent studies on the effects of dietary components and gut microbiota interactions on intestinal barrier function and systemic inflammation.

Effects of Acute Stress on Metabolic Interactions Related to the Tricarboxylic Acid (TCA) Cycle in the Left Hippocampus of Mice.

Background/objectives: The acute stress response affects brain metabolites closely linked to the tricarboxylic acid (TCA) cycle. This response involves time-dependent changes in hormones and neurotransmitters, which contribute to resilience and the ability to adapt to acute stress while maintaining homeostasis. This physiological mechanism of metabolic dynamics, combined with time-series analysis, has prompted the development of new methods to observe the relationship between TCA cycle-related brain metabolites.

Sex and Age Differences in the Impact of Metabolic Syndrome on Heart Failure Development.

Metabolic syndrome (MetS), a cluster of metabolic dysregulations, is recognized as a significant risk factor for the development of heart failure (HF). The pathophysiological mechanisms linking MetS to HF are complex and multifaceted, with the components of MetS contributing to cardiac deterioration through impaired myocardial energy metabolism, increased inflammation, and endothelial dysfunction. Numerous clinical studies have confirmed the relationship between MetS and HF.

Effect of a Mating Type Gene Editing in Using RNP/Nanoparticle Complex.

Gene editing using CRISPR/Cas9 is an innovative tool for developing new mushroom strains, offering a promising alternative to traditional breeding methods that are time-consuming and labor-intensive. However, plasmid-based gene editing presents several challenges, including the need for selecting appropriate promoters for Cas9 expression, optimizing codons for the Cas9 gene, the unintended insertion of fragmented plasmid DNA into genomic DNA (gDNA), and regulatory concerns related to genetically modified organisms (GMOs). To address these issues, we utilized a Ribonucleoprotein (RNP) complex consisting of Cas9 and gRNA for gene editing to modify the A mating-type gene of .

Targeting Reactive Oxygen Species for Diagnosis of Various Diseases.

Reactive oxygen species (ROS) are generated predominantly during cellular respiration and play a significant role in signaling within the cell and between cells. However, excessive accumulation of ROS can lead to cellular dysfunction, disease progression, and apoptosis that can lead to organ dysfunction. To overcome the short half-life of ROS and the relatively small amount produced, various imaging methods have been developed, using both endogenous and exogenous means to monitor ROS in disease settings.

Microstructural and Surface Texture Evaluation of Orthodontic Microimplants Covered with Bioactive Layers Enriched with Silver Nanoparticles.

Bacterial infections are a common cause of clinical complications associated with the use of orthodontic microimplants. Biofilm formation on their surfaces and subsequent infection of peri-implant tissues can result in either exfoliation or surgical removal of these medical devices. In order to improve the properties of microimplants, hybrid coatings enriched with silver nanoparticles, calcium, and phosphorus were investigated.

Characterisation of High Alkaline-Tolerant Novel Ulvan Lyase from : Potential Applications of Enzyme Derived Oligo-Ulvan as Anti-Diabetic Agent.

Green algae, particularly species, are rich in complex polysaccharides, such as ulvan, which have significant potential for biotechnological applications. However, the biochemical properties of ulvan depolymerised products remain underexplored. The enzymatic depolymerisation of ulvan has garnered attention owing to its cost advantages over alternative methods.

Comparative Analysis of the Biochemical and Molecular Responses of to Nitrogen and Phosphorus Limitation: Phosphorus Limitation Enhances Carotenogenesis.

is well known for its potential for biofuel production due to its high lipid content. Numerous omics and biochemical studies have explored the overall molecular mechanisms underlying the responses of sp. to nutrient availability, primarily focusing on lipid metabolism.

The Beneficial Roles of Seaweed in Atopic Dermatitis.

Atopic dermatitis (AD) is a chronic, inflammatory skin condition characterized by severe pruritus and recurrent flare-ups, significantly impacting patients' quality of life. Current treatments, such as corticosteroids and immunomodulators, often provide symptomatic relief but can lead to adverse effects with prolonged use. Seaweed, a sustainable and nutrient-dense resource, has emerged as a promising alternative due to its rich bioactive compounds-polysaccharides, phlorotannins, polyphenols, and chlorophyll-that offer anti-inflammatory, antioxidant, and immunomodulatory properties.

Lichenase and Cellobiohydrolase Activities of a Novel Bi-Functional β-Glucanase from the Marine Bacterium sp. J103.

In this study, we report the molecular and enzymatic characterisation of Spg103, a novel bifunctional β-glucanase from the marine bacterium sp. J103. Recombinant Spg103 (rSpg103) functioned optimally at 60 °C and pH 6.

Fucosterol, a Phytosterol of Marine Algae, Attenuates Immobilization-Induced Skeletal Muscle Atrophy in C57BL/6J Mice.

The objective of this study was to examine whether fucosterol, a phytosterol of marine algae, could ameliorate skeletal muscle atrophy in tumor necrosis factor-alpha (TNF-α)-treated C2C12 myotubes and in immobilization-induced C57BL/6J mice. Male C57BL6J mice were immobilized for 1 week to induce skeletal muscle atrophy. Following immobilization, the mice were administrated orally with saline or fucosterol (10 or 30 mg/kg/day) for 1 week.

Optimized Extraction of Sargahydroquinoic Acid, Major Bioactive Substance, from Using Response Surface Methodology.

Sargahydroquinoic acid (SHQA), a bioactive compound found in certain species, exhibits significant health benefits. This study optimized the extraction of SHQA from using response surface methodology (RSM) and evaluated its antioxidant effects through in vitro and in vivo assays. A Box-Behnken design (BBD) was effectively employed to investigate the effects of incubation temperature, time, and ethanol concentration on SHQA yield, achieving a high coefficient of determination (R = 0.