RAD-Seq analysis of typical and minor accessions, including Bhutanese varieties.

We analyzed the reduced-representation genome sequences of species by double-digest restriction site-associated DNA sequencing (ddRAD-Seq) using 44 accessions, including typical and minor accessions, such as Bhutanese varieties. The results of this analysis using typical accessions were consistent with previous reports that citron, papeda, pummelo, and mandarin are ancestral species, and that most species are derivatives or hybrids of these four species. varieties often reproduce asexually and heterozygosity is highly conserved within each variety.

Characterization of limes (Citrus aurantifolia) grown in Bhutan and Indonesia using high-throughput sequencing.

Lime [Citrus aurantifolia (Cristm.) Swingle] is a Citrus species that is a popular ingredient in many cuisines. Some citrus plants are known to originate in the area ranging from northeastern India to southwestern China.

Sheep genetic diversity in Bhutan using microsatellite markers.

Genotype data from eight microsatellite markers were used to assess genetic diversity and relationships among five indigenous Bhutanese sheep populations, Sakten, Jakar, Sarpang, Sipsu and Tsirang. Estimates of mean observed and expected heterozygosities, mean number of alleles per locus/population were obtained. The highest observed heterozygosities were found in Jakar (0.