Development of Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Detecting Feline Coronavirus.

Feline infectious peritonitis (FIP) is a worldwide fatal disease caused by a mutant feline coronavirus (FCoV). Simple and efficient molecular detection methods are needed. Here, sensitive, specific, rapid, and reliable colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) was developed to detect the ORF1a/1b gene of FCoV from cats with suspected FIP using neutral red as an indicator.

Using bioelectrical impedance analysis for modeling regression and predicting body fat accumulation in dogs in East Thailand.

Background And Aim: Obesity in dogs leads to several health problems, such as premature death, and contributes to other diseases. Recently, body fat percentage has been considered to represent the body condition of dogs, and bioelectrical impedance analysis (BIA) is the most effective method for accurately measuring body fat in dogs. In Thailand, information on the body condition of dogs is limited, and there is no standard body fat level for Thai or mongrel dogs.

A bi-objective optimization model for the medical supplies' simultaneous pickup and delivery with drones.

In the COVID-19 pandemic, it is essential to transport medical supplies to specific locations accurately, safely, and promptly on time. The application of drones for medical supplies delivery can break ground traffic restrictions, shorten delivery time, and achieve the goal of contactless delivery to reduce the likelihood of contacting COVID-19 patients. However, the existing optimization model for drone delivery is cannot meet the requirements of medical supplies delivery in public health emergencies.

A novel cross-species differential tumor classification method based on exosome-derived microRNA biomarkers established by human-dog lymphoid and mammary tumor cell lines' transcription profiles.

Background And Aim: Exosome-derived microRNA (miRNA) has been widely studied as a non-invasive candidate biomarker for tumor diagnosis in humans and dogs. Its application, however, was primarily focused on intraspecies usage for individual tumor type diagnosis. This study aimed to gain insight into its application as a cross-species differential tumor diagnostic tool; we demonstrated the process of identifying and using exosome-derived miRNA as biomarkers for the classification of lymphoid and mammary tumor cell lines in humans and dogs.

Predicting nursing students' psychological well-being: network analysis based on a model of thriving through relationships.

Background: Psychological well-being plays a vital role in nursing students' mental health and affects their decisions to stay in the nursing profession, particularly during the COVID-19 outbreak. Close relationships are undeniably linked to psychological well-being, but it is unknown how the specific pathways through which close relationships are related to each other and which are most strongly linked to nursing students' psychological well-being.

Aims: To explore the network structure, central and bridge factors among well-being characteristics, and predictors based on a model of thriving through relationships.

Perceived Social Support Promotes Nursing Students' Psychological Wellbeing: Explained With Self-Compassion and Professional Self-Concept.

Background: The psychological distress of nursing students is ongoing and getting worse during the coronavirus disease 2019 (COVID-19) outbreak. Numerous calls for future research on exploring the effects of perceived social support would be an effective way to improve nursing students' mental health. However, the pathway(s) between perceived social support and psychological wellbeing (PWB) remain unknown.

Z-Bar Shoeing Demonstrates Potential for Long-Term Foot Pain Management during an Exercise Training Regimen in a Show Jumping Pony with Uniaxial Palmar Pain.

Z-bar shoeing has been implemented to relieve uniaxial palmar pain arising from the structures in the affected region. However, there have been no reports on the long-term application of the z-bar shoe during exercise training regimens. A 10-year-old mixed-breed show jumping pony presented with an occasional short stride and abnormal rhythm while turning during routine exercise for three months.

Data Driven for Early Breast Cancer Staging using Integrated Mammography and Biopsy.

Objective: Breast cancer patients who have a rapid diagnosis have been better prognosis than late diagnosis. The popular screening is mammogram or ultrasound. In recent years, researchers try to develop data driven models to predict early cancer staging from the first screening.

Effect of Mineral Salt Blocks Containing Sodium Bicarbonate or Selenium on Ruminal pH, Rumen Fermentation and Milk Production and Composition in Crossbred Dairy Cows.

Ruminal pH is an important physiological parameter that regulates microbe activity; optimizing ruminal pH may improve rumen fermentation and milk production. The purpose of this experiment was to determine the effect of sodium bicarbonate (NaHCO) or selenium (Se) in mineral salt block (MSB) supplementation on ruminal pH, rumen fermentation, milk yield and composition in Holstein Friesian crossbred dairy cows. Four crossbred dairy cows with an initial weight of 456 ± 6 kg in mid-lactation were assigned at random using a 4 × 4 Latin square design.

Blood meal analysis of tsetse flies ( : Glossinidae) reveals higher host fidelity on wild compared with domestic hosts.

Changes in climate and land use can alter risk of transmission of parasites between domestic hosts and wildlife, particularly when mediated by vectors that can travel between populations. Here we focused on tsetse flies (genus ), the cyclical vectors for both Human African Trypanosomiasis (HAT) and Animal African Trypanosomiasis (AAT). The aims of this study were to investigate three issues related to from Kenya: 1) the diversity of vertebrate hosts that flies fed on; 2) whether host feeding patterns varied in relation to type of hosts, tsetse feeding behaviour, site or tsetse age and sex; and 3) if there was a relationship between trypanosome detection and host feeding behaviours or host types.

Potential antibiotic production of sp. nov., isolated from the root of .

Endophytic actinobacterial strain 3R004 was isolated from a root of collected in Thailand. In this report, the taxonomic position of this strain is described using a polyphasic approach. Based on the morphological characteristics and chemical composition of its cells, strain 3R004 was identified as a member of the genus .

Recycling rice husk for removal of phosphate and nitrate from synthetic and swine wastewater: Adsorption study and nutrient analysis of modified rice husk.

The objective of this study was to determine the adsorbent potential of rice husk and its modified form for phosphate and nitrate removal from synthetic and swine-farm wastewater. The mechanism of adsorption as well as the potential of phosphate-/nitrate- adsorbed rice husk as nutrient rich residue was also investigated. Two-step modification of RH (using base-washing (BW) and chemical modification (CM) was conducted to compare the phosphate and nitrate removal.

Scorpion Venom Peptide Effects on Inhibiting Proliferation and Inducing Apoptosis in Canine Mammary Gland Tumor Cell Lines.

The most common neoplasms in intact female dogs are CMGTs. BmKn-2, an antimicrobial peptide, is derived from scorpion venom and has published anticancer effects in oral and colon human cancer cell lines. Thus, it is highly likely that BmKn-2 could inhibit CMGT cell lines which has not been previously reported.

Seasonal effect on semen availability and quality of racing pigeon in Thailand.

Background And Aim: Seasonal variations among geographical regions could influence pigeon semen quality differently. This study aimed to determine the seasonal effect on semen availability and quality of racing pigeons in Thailand to understand and improve breeding management in the country.

Materials And Methods: Semen was collected from six fertile captive pigeons once a week during summer (March-June), monsoon (July-October), and winter (November-February) during 2019-2020.

Comparative assessment of immunochromatographic test kits using somatic antigens from adult Opisthorchis viverrini and IgG and IgG4 conjugates for serodiagnosis of human opisthorchiasis.

Chronic infections of humans with Opisthorchis viverrini and Clonorchis sinensis spanning decades may lead to life-threatening pathology prior to cholangiocarcinoma (CCA), which usually has a poor prognosis. Serological tools can support the parasitological examination in clinical diagnosis and support screening for risk of CCA. We developed novel immunochromatographic test kits using a soluble, somatic tissue extract of adult O.

Low-cost alternative biodiesel production apparatus based on household food blender for continuous biodiesel production for small communities.

Fatty acid methyl esters (FAMEs) are sustainable biofuel that can alleviate high oil costs and environmental impacts of petroleum-based fuel. A modified 1200 W high-efficiency food blender was employed for continuous transesterification of various refined vegetable oils and waste cooking oil (WCO) using sodium hydroxide as a homogeneous catalyst. The following factors have been investigated on their effects on FAME yield: baffles, reaction volume, total reactant flow rate, methanol-oil molar ratio, catalyst concentration and reaction temperature.

Support vector regression algorithm modeling to predict the parturition date of small - to medium-sized dogs using maternal weight and fetal biparietal diameter.

Background And Aim: Fetal biparietal diameter (BPD) is a feasible parameter to predict canine parturition date due to its inverted correlation with days before parturition (DBP). Although such a relationship is generally described using a simple linear regression (SLR) model, the imprecision of this model in predicting the parturition date in small- to medium-sized dogs is a common problem among veterinarian practitioners. Support vector regression (SVR) is a useful machine learning model for prediction.

Development and application of a novel multiplex PCR assay for the differentiation of four haemosporidian parasites in the chicken Gallus gallus domesticus.

Haemosporidian infections in domestic chickens (Gallus gallus domesticus) are not only widely prevalent but also cause economic loss. Diagnosis is usually made by microscopic examination; however, the method has several drawbacks such as requiring an experienced microscopist, being unreliable when parasitemia is low and being unable to accurately differentiate between co-infections from multiple parasite species. Therefore, the current extent of haemosporidian infections might be underestimated and neglected.

High prevalence of opisthorchiasis in rural populations from Khammouane Province, central Lao PDR: serological screening using total IgG- and IgG4-based ELISA.

Background: Human opisthorchiasis, caused by Opisthorchis viverrini, is a public health problem in Southeast Asia and a major risk factor for cholangiocarcinoma. In Lao PDR, seroprevalence and the relationship between the number of O. viverrini eggs in infected people and specific antibody responses are still unknown.

Influence of full-time housing in vector-protected facilities on equine cortisol levels, heart rate, and behavior during the African horse sickness outbreak in Thailand.

Horses were provided full-time housing in unfamiliar vector-protected facilities during the African horse sickness (AHS) outbreak in Thailand. This study aimed to investigate the impact of this housing arrangement on the equine stress response. Nine healthy horses were housed in both a traditional barn and a vector-protected barn.

Fetal mortality associated with backfat thickness at first mating and first farrowing of the primiparous sows raised in a commercial herd in Thailand.

This study aimed to investigate the fetal mortality, including stillborn piglets (SB) and mummified fetuses (MM), in relation to backfat thickness both at first mating (MBF) and at first farrowing (FBF) in 200 primiparous sows accommodated in a commercial breeding herd in Thailand. Backfat thickness of all pigs was measured at P2 position using an A-mode ultrasonography. Based on MBF, the gilts were classified into four groups: MBF1 (≤12.

Comparative karyotype study of three Cyprinids (Cyprinidae, Cyprininae) in Thailand by classical cytogenetic and FISH techniques.

Three species of ornamental fishes in the subfamily Cyprininae (family Cyprinidae) namely, (Fowler, 1934), (Fowler, 1934), Boonyaratpalin et Srirungroj, 1971 were studied by classical cytogenetic and fluorescent in situ hybridization (FISH) techniques. Chromosomes were directly prepared from kidney tissues and stained by using conventional and Ag-NOR banding techniques. Microsatellite d(CA) and d(CGG) probes were hybridized to the chromosomes of three cyprinids.

Chromosomes of Asian cyprinid fishes: Variable karyotype patterns and evolutionary trends in the genus Osteochilus (Cyprinidae, Labeoninae, "Osteochilini").

The Cyprinidae family is a highly diversified but demonstrably monophyletic lineage of cypriniform fishes. Among them, the genus Osteochilus contains 35 recognized valid species distributed from India, throughout Myanmar, Laos, Thailand, Malaysia, Indonesian archipelago to southern China. In this study, karyotypes and other chromosomal characteristics of five Osteochilus species occurring in Thailand, namely O.

Comparison of three filter paperbased devices for safety and stability of viral sample collection in poultry.

General diagnosis of poultry viruses primarily relies on detection of viruses in samples, but many farms are located in remote areas requiring logistic transportation. Filter paper cards are a useful technology that offer an alternative for collecting and preserving samples without hazardous exposure. The goal of this study was to compare three filter papers: the Flinders Technology Associates filter (FTA®) card, dried blood spot (DBS) card and qualitative filter paper (FP) grade 2 to collect poultry samples.

Differential DNA methylation analysis across the promoter regions using methylated DNA immunoprecipitation sequencing profiling of porcine loin muscle.

Background And Aim: Pork leanness and marbling are among the essential traits of consumer preference. To acquire knowledge about universal epigenetic regulations for improving breed selection, a meta-analysis of methylated DNA immunoprecipitation sequencing (MeDIP-seq) profiling data of mixed loin muscle types was performed in this study.

Materials And Methods: MeDIP-seq profiling datasets of longissimus dorsi muscle and psoas major muscles from male and female pigs of Landrace and Tibetan breeds were preprocessed and aligned to the porcine genome.