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Lectin-microarray technique for glycomic profiling of fungal cell surfaces.
Methods Mol Biol
April 2015
Laboratory for Inflammatory Regulation, RIKEN Research Center for Integrative Medical Science (IMS-RCAI), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.
Lectin microarrays are rows of lectins with different carbohydrate-binding specificities spotted on surfaces of glass slides. Lectin microarray technique enables glycomic analyses of carbohydrate composition of fungal cell walls. We will describe an application of the technique in analyzing cell surface glycome of yeast-form fungal cells in the living state.
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