LncRNA induces p53 degradation by interfering with the interaction between p53 and the SART3-USP15 complex.

Mammalian genomes encode large number of long noncoding RNAs (lncRNAs) that play key roles in various biological processes, including proliferation, differentiation, and stem cell pluripotency. Recent studies have addressed that some lncRNAs are dysregulated in human cancers and may play crucial roles in tumor development and progression. Here, we show that the lncRNA is required for the proliferation and tumorigenicity of colon cancer cells with wild-type p53.

Selection for 3'-end triplets for polymerase chain reaction primers.

Primer extension by thermostable DNA polymerase in PCR starts from the 3'-end of a primer. If the PCR starting process fails, the entire PCR fails. Primer sequences at the 3'-end often interfere with success in PCR experiments.