An Efficient and Cost-Effective Novel Strategy for Identifying CRISPR-Cas-Mediated Mutants in Plant Offspring.

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 system has revolutionized targeted mutagenesis, but screening for mutations in large sample pools can be time-consuming and costly. We present an efficient and cost-effective polymerase chain reaction (PCR)-based strategy for identifying edited mutants in the T generation. Unlike previous methods, our approach addresses the challenges of large progeny populations by using T generation sequencing results for genotype prediction.

Five-Year Results With Patisiran for Hereditary Transthyretin Amyloidosis With Polyneuropathy: A Randomized Clinical Trial With Open-Label Extension.

Importance: There is a lack of long-term efficacy and safety data on hereditary transthyretin amyloidosis with polyneuropathy (hATTR-PN) and on RNA interference (RNAi) therapeutics in general. This study presents the longest-term data to date on patisiran for hATTR-PN.

Objective: To present the long-term efficacy and safety of patisiran in adults with hATTR-PN.

Electrodeposited Nitrate-Intercalated NiFeCe-Based (Oxy)hydroxide Heterostructure as a Competent Electrocatalyst for Overall Water Splitting.

Electrochemical water splitting is a promising method for the generation of "green hydrogen", a renewable and sustainable energy source. However, the complex, multistep synthesis processes, often involving hazardous or expensive chemicals, limit its broader adoption. Herein, a nitrate (NO) anion-intercalated nickel-iron-cerium mixed-metal (oxy)hydroxide heterostructure electrocatalyst is fabricated on nickel foam (NiFeCeOH@NF) via a simple electrodeposition method followed by cyclic voltammetry activation to enhance its surface properties.

A Novel High-Conjugated Probe of 4-(Acridone-10-yl)-Phenylethyl Chloroformate (APE-Cl) for Rapid Detection of Amino Compounds Using HPLC with Fluorescence Detection.

The fluorescence detection of amino compounds and the evaluation of their content in environmental samples are vital, not only for assessing food quality but also for studying soil organic matter. Here, we present the synthesis and application of a novel fluorescent probe, 4-(9-acridone)benzylmethyl carbonochloride (APE-Cl), for detecting amino compounds via a chloroformate reaction with fluorescence detection. The complete derivatization reaction of APE-Cl with amino compounds can be accomplished in aqueous acetonitrile within 5 min at room temperature, using 0.

Mercury exposure in an endangered songbird: influence of marsh hydrology and evidence for early breeding impairment.

Songbird reproductive success can decline from consuming mercury-contaminated aquatic insects, but assessments of hydrologic conditions influencing songbird mercury exposure are lacking. We monitored breast feather total mercury (THg) concentrations and reproductive success in the U.S.

Patterns and predictors of statin therapy after ischemic stroke and TIA: insights from the LIPYDS multicenter study.

Background: Patients with ischemic stroke (IS) or TIA face an elevated cardiovascular risk, warranting intensive lipid-lowering therapy. Despite recommendations, adherence to guidelines is suboptimal, leading to frequent undertreatment. This study aims to evaluate the statin use after IS and TIA.

Automatic segmentation model and machine learning model grounded in ultrasound radiomics for distinguishing between low malignant risk and intermediate-high malignant risk of adnexal masses.

Objective: To develop an automatic segmentation model to delineate the adnexal masses and construct a machine learning model to differentiate between low malignant risk and intermediate-high malignant risk of adnexal masses based on ovarian-adnexal reporting and data system (O-RADS).

Methods: A total of 663 ultrasound images of adnexal mass were collected and divided into two sets according to experienced radiologists: a low malignant risk set (n = 446) and an intermediate-high malignant risk set (n = 217). Deep learning segmentation models were trained and selected to automatically segment adnexal masses.

Emerging Trends in Neuroblastoma Diagnosis, Therapeutics, and Research.

This review explores the current understanding and recent advancements in neuroblastoma, one of the most common extracranial solid pediatric cancers, accounting for ~ 15% of childhood cancer-related mortality. The hallmarks of NBL, including angiogenesis, metastasis, apoptosis resistance, cell cycle dysregulation, drug resistance, and responses to hypoxia and ROS, underscore its complex biology. The tumor microenvironment's significance in disease progression is acknowledged in this study, along with the pivotal role of cancer stem cells in sustaining tumor growth and heterogeneity.

Association Between Diagnostic Delay and Economic and Clinical Burden in Axial Spondyloarthritis: A Multicentre Retrospective Observational Study.

Introduction: Axial spondyloarthritis (axSpA) is a chronic inflammatory condition associated with considerable pain and impaired health-related quality of life (HRQoL) for affected patients. Despite the documented increase in healthcare resource utilization (HRU) related to axSpA, few studies have explored the impact of diagnostic delays on these outcomes. This study sought to determine the association between diagnostic delay of axial spondyloarthritis (axSpA) and costs in the 3 years after diagnosis.

Low-cost male urogenital simulator for penile implant surgery training: a 3D printing approach.

Background: Penile implant surgery is the standard surgical treatment for end-stage erectile dysfunction. However, the growing complexity of modern high-tech penile prostheses has increased the demand for more practical training opportunities. The most advanced contemporary training methods involve simulation training using cadavers, with costs exceeding $5,000 per cadaver, inclusive of biohazard fees.

Factors influencing spatiotemporal variability of NO concentration in urban area: a GIS and remote sensing-based approach.

The growing global attention on urban air quality underscores the need to understand the spatiotemporal dynamics of nitrogen dioxide (NO) and its environmental and anthropogenic factors, particularly in cities like Dhaka (Gazipur), Bangladesh, which suffers from some of the world's worst air quality. This study analysed NO concentrations in Gazipur from 2019 to 2022 using Sentinel-5P TROPOMI data on the Google Earth Engine (GEE) platform. Correlations and regression analysis were done between NO levels and various environmental factors, including land surface temperature (LST), normalized difference vegetation index (NDVI), land use and land cover (LULC), population density, road density, settlement density, and industry density.

A Theoretical Study on Crossings Among Electronically Excited States and Laser Cooling of Group VIA (S, Se, and Te) Hydrides.

Various electronically excited states and the feasibility of direct laser cooling of SH, SeH, and TeH are investigated using the highly accurate ab initio and dynamical methods. For the detailed calculations of the seven low-lying Λ-S states of SH, we utilized the internally contracted multireference configuration interaction approach, considering the spin-orbit coupling (SOC) effects. Our calculated spectroscopic constants are in very good agreement with the available experimental results.

Pulmonary Dysfunction in Children with Dystrophinopathy: A Cross-Sectional Study.

Objectives: To observe the prevalence of impaired pulmonary function during various phases of the disease course in children aged 5-18 y with dystrophinopathy. The correlation between different parameters of pulmonary dysfunction and motor function was also studied.

Methods: One hundred and thirty-three confirmed cases of Duchenne muscular dystrophy (DMD), fulfilling predefined inclusion and exclusion criteria were evaluated.

Integration of silver nanostructures in wireless sensor networks for enhanced biochemical sensing.

Integrating noble metal nanostructures, specifically silver nanoparticles, into sensor designs has proven to enhance sensor performance across key metrics, including response time, stability, and sensitivity. However, a critical gap remains in understanding the unique contributions of various synthesis parameters on these enhancements. This study addresses this gap by examining how factors such as temperature, growth time, and choice of capping agents influence nanostructure shape and size, optimizing sensor performance for diverse conditions.

A Review of the Prospera Spinal Cord Stimulation System with Multiphase Stimulation and Proactive Care.

Purpose Of Review: The purpose of this review is to describe the development and key features of the Prospera™ Spinal Cord Stimulation (SCS) System, as well as the clinical evidence supporting its use. Prospera delivers therapy using a proprietary multiphase stimulation paradigm and is the first SCS system to offer proactive care through automatic, objective, daily, remote device monitoring and remote programming capabilities.

Recent Findings: Results from the recently published BENEFIT-02 trial support the short-term safety and efficacy of multiphase stimulation in patients with chronic pain.

PARADIGM-PV: a randomized, multicenter phase 4 study to assess the efficacy and safety of ropeginterferon alfa-2b in patients with low- or high-risk polycythemia vera.

Polycythemia vera (PV) is characterized by clonal hematopoietic stem or progenitor cells with constitutively active somatic mutation(s) in the Janus kinase 2 gene. Phlebotomy (Phl) and aspirin are often used alone for low-risk PV patients. However, data from the Low-PV study demonstrated that Phl and aspirin may not be adequate for patients.

Associations of personality trait level and change with mortality risk in 11 longitudinal studies.

People who are higher in conscientiousness, extraversion, and agreeableness and lower in neuroticism tend to live longer. The present research tested the hypothesis that personality trait change in middle and older adulthood would also be associated with mortality risk, above and beyond personality trait level. Personality trait change may causally influence mortality risk through corresponding changes in health behaviors, social processes, and stress experience.

Correction to "A grain of truth in the grain size effect: Retrieval practice is more effective when interspersed during learning" by Don et al. (2024).

Reports an error in "A grain of truth in the grain size effect: Retrieval practice is more effective when interspersed during learning" by Hilary J. Don, Shaun Boustani, Chunliang Yang and David R. Shanks (, 2024[Nov], Vol 50[11], 1791-1810).

Electrochemical Cyclizative Carboxylation of Alkene-Tethered Aryl Isocyanides with Carbon Dioxide.

Herein, we present an unprecedented electrochemical reductive cyclizative carboxylation of -vinylphenyl isocyanides with carbon dioxide achieved without the use of metal catalysts. This protocol demonstrates a broad substrate scope and good functional group tolerance, facilitating the rapid assembly of 2-oxoindolin-3-acetic acids in good to high yields with excellent regioselectivity. Furthermore, these structural motifs may have potential applications in formal synthesis of bioactive natural products.

A Case-Control Study of the Association Between Gene Polymorphisms (rs1695 and rs1138272) and the Susceptibility to Male Infertility in the Moroccan Population.

Infertility affects 10-15% of couples worldwide, with male factors accounting for half of cases. Environmental, behavioral, and genetic problems contribute to spermatogenic failure in 30% of idiopathic male infertility cases. Other factors, such as oxidative stress (OS), cause impaired spermatogenesis, abnormal sperm morphology, and reduced motility, eventually triggering male infertility.

Highly polarized single-crystal organic light-emitting devices with low turn-on voltage and high brightness.

Linearly-polarized organic electroluminescent devices have gained significant attention due to their potential applications across various fields. However, traditional thin-film organic light-emitting diodes (OLEDs) face significant challenges, primarily due to the necessity of incorporating complex optical elements. In this study, we present linearly-polarized OLEDs (LP-OLEDs) based on organic single crystals that we have designed and prepared.

Intermetallic RNiSi (R = Ca, La, and Y) Catalysts with Electron-Rich Ni Sites for Continuous Flow Selective Hydrogenation of Maleic Anhydride.

The industrial advancement of downstream products resulting from the directed hydrogenation of maleic anhydride is hindered by the limitations related to the activity and stability of catalysts. The development of nonprecious metal intermetallic compounds, in which active sites are adjustable in the local structures and electronic properties embedded within a distinct framework, holds immense potential in enhancing catalytic efficacy and stability. Herein, we report that nickel-based silicides catalysts, RNiSi (R = Ca, La, and Y), afford high efficiency in the selective hydrogenation of maleic anhydride.

A Hexavalent Tellurium-Based Chalcogen Bonding Catalysis Platform: High Catalytic Activity and Controlling of Selectivity.

Herein, we describe a hexavalent tellurium-based chalcogen bonding catalysis platform capable of addressing reactivity and selectivity issues. This research demonstrates that hexavalent tellurium salts can serve as a class of highly active chalcogen bonding catalysts for the first time. The tellurium centers in these hexavalent catalysts have only one exposed interaction site, thus providing a favorable condition for the controlling of reaction selectivity.