Microbial oil production from acidified glycerol pretreated sugarcane bagasse by .

An integrated microbial oil production process consisting of acidified glycerol pretreatment of sugarcane bagasse, enzymatic hydrolysis, microbial oil production by NRRL 1757 and oil recovery by hydrothermal liquefaction (HTL) of fungal biomass in fermentation broth was assessed in this study. Following pretreatment, the effect of residual pretreatment hydrolysate (containing glycerol) on enzymatic hydrolysis was firstly studied. The residual pretreatment hydrolysate (corresponding to 2.